Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 380,004 A→C 5.2% intergenic (‑123/+65) frmR ← / ← yaiO regulator protein that represses frmRAB operon/outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913380,0040AC 5.2% 580.3 / 2.1 183intergenic (‑123/+65)frmR/yaiOregulator protein that represses frmRAB operon/outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (139/30);  new base C (2/12);  total (141/42)
Fisher's exact test for biased strand distribution p-value = 3.47e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 198 total aligned reads displayed.

TTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTC  >  NC_000913/379856‑380153
                                                                                                                                                    |                                                                                                                                                       
ttcttcTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACaaa                                                                                                                                                        <  2:1604123/150‑1 (MQ=255)
ttcttcTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACaaa                                                                                                                                                        <  1:4395018/150‑1 (MQ=255)
ttcttcTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACaaa                                                                                                                                                        <  2:5796184/150‑1 (MQ=255)
 tcttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAag                                                                                                                                                       <  2:2012303/150‑1 (MQ=255)
 tcttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAag                                                                                                                                                       >  1:100199/1‑150 (MQ=255)
 tcttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAag                                                                                                                                                       >  1:229438/1‑150 (MQ=255)
  cttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAaga                                                                                                                                                      <  2:3452675/150‑1 (MQ=255)
  cttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAaga                                                                                                                                                      <  1:5087866/150‑1 (MQ=255)
  cttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAAGATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAaga                                                                                                                                                      <  2:4495837/150‑1 (MQ=255)
   ttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCGATAGTATATAACACTGGAGAATAAAAGTTATCCGGTGAAGGTGGTCGGACAACAAagag                                                                                                                                                     >  1:5169929/1‑150 (MQ=255)
      tcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGaa                                                                                                                                                  >  1:1518288/1‑150 (MQ=255)
       cttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGaaa                                                                                                                                                 <  1:2764131/150‑1 (MQ=255)
         tcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTAAAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑gg                                                                                                                                             >  2:5973862/1‑150 (MQ=255)
               gTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGATAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑gggggggg                                                                                                                                       <  2:1013457/150‑1 (MQ=255)
               gTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTAGAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑gggggggg                                                                                                                                       <  2:2522388/150‑1 (MQ=255)
                   tGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTCTTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCt                                                                                                                                   >  1:678806/1‑150 (MQ=255)
                   tGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑gggggggggggt                                                                                                                                   >  1:5546051/1‑148 (MQ=255)
                   tGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCt                                                                                                                                   >  2:2606883/1‑150 (MQ=255)
                    gggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACACAGAGGACA‑‑GGGGGGGGGGCTa                                                                                                                                  >  1:5745522/1‑150 (MQ=255)
                     ggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTaa                                                                                                                                 >  1:761706/1‑150 (MQ=255)
                     ggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTaa                                                                                                                                 >  1:1083617/1‑150 (MQ=255)
                      gtttttCGAACCTTATCTTCTTCTTTCTTTATACTTTTGGGGTGTTTTCTATATTTCATTGATTACCCCGGTTTTTTTTATAACCCTGTATATTAAATTTTAGCCGGTGATTGTGGTGGGAAAACAACGCGGAAA‑‑GGGGGGGGGGCTAAt                                                                                                                                <  2:526539/147‑1 (MQ=255)
                      gCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAAt                                                                                                                                >  1:1860393/1‑150 (MQ=255)
                       cATTTCGCACCTCATCATCTGCATGCAATATACTATTGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTTTATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGTAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATc                                                                                                                               <  2:1197088/150‑1 (MQ=255)
                       cATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATc                                                                                                                               >  1:4749721/1‑150 (MQ=255)
                       cATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATc                                                                                                                               >  2:5578424/1‑150 (MQ=255)
                         tttCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCgg                                                                                                                             >  1:3770744/1‑150 (MQ=255)
                          ttCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATACCACTGTAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAACGCGGAAA‑‑GGGGGGGGGGCTAATGGGc                                                                                                                            <  2:739581/150‑1 (MQ=255)
                           tCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCGAATCGGcg                                                                                                                           >  2:1907197/1‑149 (MQ=255)
                              cACCTCATCATCTTAATGCAATATACTATCTGGGGGTTTTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGATAATAAAATTTATCCGGTGTATTTGGTCTGAAAACAAAGAGTAAA‑‑GGGGGGGGGGCTAATCGGCAggg                                                                                                                        <  2:5902520/150‑1 (MQ=255)
                               aCCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGa                                                                                                                       >  2:2352153/1‑150 (MQ=255)
                                 ctactcatcTGCATGCAATATACTATCGGGGGGTATTCAATATCTCAATGCATACCCCCCAATAGTATATAACACTGGAGAATAAACTTGCTCCGGTGCCTGTGGCCGGAAACCAAAGCCCAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAg                                                                                                                     <  2:5946105/146‑1 (MQ=255)
                                  tcatcaTCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAgg                                                                                                                    >  2:2959976/1‑150 (MQ=255)
                                  tcatcaTCTCCATGCAATATACTATAGGGGGGTATTCTATATGTAAATGCATACCCCCCTTTAGTATATAACACTGGAGAATAAAATTCATCCGGTGAATGTGGTCGGAAAACAACGCGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAgg                                                                                                                    <  2:3666021/150‑1 (MQ=255)
                                   catcatCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGgc                                                                                                                   >  1:1463790/1‑150 (MQ=255)
                                     tcatcTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGgccg                                                                                                                 >  1:866498/1‑150 (MQ=255)
                                        gcTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTACCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGccc                                                                                                              <  2:797606/149‑1 (MQ=255)
                                         cTGCATTCCATATACTATCGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACCCTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACCACGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGcccc                                                                                                             <  2:1111578/150‑1 (MQ=255)
                                          tgcatgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGGCTAAGCGGCGGGGAGGGCCGccc                                                                                                              >  1:4460602/1‑150 (MQ=255)
                                           gcatgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCgg                                                                                                           >  2:3310213/1‑150 (MQ=255)
                                               gcaCTATACTCTCGGGGGGTATTCTATATGCCAACCCATACCCCCCTATAGTATCCACCACTGGAGAAAAAACTTTATCCCGCGACTGCGGCCGGCCACCACCCAGGAAC‑‑GGGGGGGGGGCCAATCGGCAGGGAAGGCCGCCCCGGATAg                                                                                                       <  2:5845137/150‑1 (MQ=255)
                                                       cTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGggcggc                                                                                               >  1:4512296/1‑150 (MQ=255)
                                                           aGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAg                                                                                          >  2:5682437/1‑150 (MQ=255)
                                                           aGGGGGGTATTCTATATGTCAATGCACACCCCCCTATAGTATATAACCCTGGAGAATAAACTTTCTCCGGTGACTGTGGTCGGAACACAAAGAGGAAC‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGaa                                                                                           <  2:1855812/150‑1 (MQ=255)
                                                             gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAgg                                                                                         >  1:2679395/1‑150 (MQ=255)
                                                             gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAAAAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAgg                                                                                         >  2:4583269/1‑150 (MQ=255)
                                                               gggTATTCCATATGTCAATGCATACCCCCCCATAGTAGATAACACGGGCGAATAAAATTCACCCGGGGAATGTGGTCGGAAAACACAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGaa                                                                                       <  2:2253905/150‑1 (MQ=255)
                                                                ggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAAGAAAGTGGATT‑‑GGGGGGGGGGGTATTCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAt                                                                                      >  1:3813082/1‑150 (MQ=255)
                                                                ggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAt                                                                                      >  1:3508378/1‑150 (MQ=255)
                                                                    ttCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAg                                                                                   >  2:4434684/1‑150 (MQ=255)
                                                                         tatGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCCGAATTTc                                                                             >  1:1788809/1‑150 (MQ=255)
                                                                           tgccAATGCATACCCCCCTATAGTATAGAACACTGGCGAATAAACTTGCGCCGGTGAAGGTGGTCGGAAAACACCGCGGACA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCa                                                                           <  2:3187320/147‑1 (MQ=255)
                                                                           tGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCa                                                                           >  2:1643889/1‑150 (MQ=255)
                                                                            gtcaccgCATCCCCCCCTCTAGTATAGAACCCTGGAGAATAAAATGTCCCCGGGGACGGTGGCCGGAACACAACGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAg                                                                          <  2:5234025/144‑1 (MQ=255)
                                                                             tCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                                                                         >  2:1654089/1‑150 (MQ=255)
                                                                              cAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGt                                                                        >  1:2922214/1‑150 (MQ=255)
                                                                               aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACACAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCCGGGAAGGCCGCCCCGGCTAGCGGGCGGCCGAAGGACACAGAATTTCCCgggc                                                                       >  2:1411140/1‑148 (MQ=255)
                                                                               aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTc                                                                       >  2:934073/1‑150 (MQ=255)
                                                                               aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCa                                                                      >  2:1441664/1‑150 (MQ=255)
                                                                               aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGGGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCGAAACCGCCGGGAAGGCCCCCCCCGAAAGCCGGCGGCCGCAGGAAGCCGAAATTCCCGGTc                                                                       >  2:4258760/1‑150 (MQ=255)
                                                                                aTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCa                                                                      >  2:4125426/1‑150 (MQ=255)
                                                                                 tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAg                                                                     >  1:629725/1‑150 (MQ=255)
                                                                                        cccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                                              >  2:1786611/1‑150 (MQ=255)
                                                                                        cccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                                              >  2:2052903/1‑150 (MQ=255)
                                                                                        cccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                                              >  1:3021472/1‑150 (MQ=255)
                                                                                        cccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                                              >  1:4277308/1‑150 (MQ=255)
                                                                                        cccccTATAGCATATACCACGGGAGAACACCCCGCCACCGGTGACTGCGGCCGGACCCCACCGCGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                                              <  2:2994403/150‑1 (MQ=255)
                                                                                             tataGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAg                                                         >  1:4525852/1‑150 (MQ=255)
                                                                                                 gTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc                                                     >  1:4876378/1‑150 (MQ=255)
                                                                                                  tatataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                                                    >  2:508564/1‑150 (MQ=255)
                                                                                                  tatataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                                                    >  2:5041237/1‑150 (MQ=255)
                                                                                                   atataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAT‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCTGGTCAGACGGGCTGCTAGTTGCAg                                                   >  2:2951424/1‑150 (MQ=255)
                                                                                                      taACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAc                                                 >  1:1125275/1‑150 (MQ=255)
                                                                                                       aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGACTCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCg                                               >  1:5714265/1‑150 (MQ=255)
                                                                                                              gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAat                                        >  2:452583/1‑150 (MQ=255)
                                                                                                                  aTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatcatc                                    >  1:519679/1‑150 (MQ=255)
                                                                                                                   tAAAATTTATCCGGTGAATGTGGTCGGAATTCAATGTGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGTAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCg                                   >  1:1990524/1‑150 (MQ=255)
                                                                                                                   tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCg                                   >  1:2048229/1‑150 (MQ=255)
                                                                                                                     aaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGt                                 >  2:3802517/1‑150 (MQ=255)
                                                                                                                       aTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTg                               >  1:163621/1‑150 (MQ=255)
                                                                                                                           ctCCGGAGAATGTGGCCGGAAACCCCCGCGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgt                           <  2:4104644/149‑1 (MQ=255)
                                                                                                                              cGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTc                        >  2:4398563/1‑150 (MQ=255)
                                                                                                                              cGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTc                        >  1:2368082/1‑150 (MQ=255)
                                                                                                                               ggTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCg                       >  1:3479578/1‑150 (MQ=255)
                                                                                                                               ggTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCg                       >  2:5513246/1‑150 (MQ=255)
                                                                                                                               ggTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTGGGGGTGTCg                       >  2:4934279/1‑150 (MQ=255)
                                                                                                                                gTGAATGTGGTCGGAAAACAAATATGAAA‑‑GGGGGTGGGTCTAATCGGCAGGGAAGGCCGCACCGGATAGCGGTCGGCATAAGGAATCATAAATTCCAGGTCATACGCGCTGCAATTTGAAGACCGTTAAAATCAACGGTTGGGGTGTctt                      >  1:1374904/1‑148 (MQ=255)
                                                                                                                                gTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGt                      >  1:325016/1‑150 (MQ=255)
                                                                                                                                       tgGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACcacc                >  2:3922853/1‑149 (MQ=255)
                                                                                                                                         gTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACtt             >  1:3006180/1‑150 (MQ=255)
                                                                                                                                         gTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACtt             >  1:3383686/1‑150 (MQ=255)
                                                                                                                                          tCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACttt            >  1:3334390/1‑150 (MQ=255)
                                                                                                                                             gAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTAcc         >  1:4991049/1‑150 (MQ=255)
                                                                                                                                             gAAAACAAAGAGGAAA‑‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCt        >  1:2219532/1‑150 (MQ=255)
                                                                                                                                                  cAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCg    >  2:4871015/1‑150 (MQ=255)
                                                                                                                                                   aaaGAGGAAAGGGGGGGGGGGATAATCGGCAGGGAAGGCCGCCCCGGATAGAGGGCCGCAGAATGAATCAGAATTTCCAGGTCAGACGGGCAGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCg    >  2:3914074/1‑150 (MQ=255)
                                                                                                                                                   aaaGAGGAAA‑‑GGGGTGGGGGCTAATCGGCAGGGAACGACGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAATTCCAGGACAGACGGGCTGCAATTAGCATAACGTTAAAATCATCGGTAGGGGTGTCGTACCACAAATAACCTGccca   >  1:2500273/1‑148 (MQ=255)
                                                                                                                                                   aaaGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGt   >  1:4186546/1‑150 (MQ=255)
                                                                                                                                                   aaaGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGt   >  1:4643770/1‑150 (MQ=255)
                                                                                                                                                    aaGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTGGGGGTGTCGTACCACACTTTACCTGCCGTc  >  2:3084667/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                       
TTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTC  >  NC_000913/379856‑380153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: