Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 708,020 C→A 6.3% intergenic (+263/‑314) glnS → / → chiP glutamyl‑tRNA synthetase/chitoporin, uptake of chitosugars

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913708,0200CA 6.3% 1006.5 / 5.9 319intergenic (+263/‑314)glnS/chiPglutamyl‑tRNA synthetase/chitoporin, uptake of chitosugars
Reads supporting (aligned to +/- strand):  ref base C (115/169);  new base A (31/3);  total (147/172)
Fisher's exact test for biased strand distribution p-value = 1.24e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 330 total aligned reads displayed.

CTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGGTTGCT  >  NC_000913/707871‑708169
                                                                                                                                                     |                                                                                                                                                     
ctctGCAATATATTGCGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGAAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATc                                                                                                                                                       <  1:145952/150‑1 (MQ=255)
 tctGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATcc                                                                                                                                                      >  1:991601/1‑150 (MQ=255)
   tGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCat                                                                                                                                                    >  2:6000617/1‑150 (MQ=255)
    gCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCata                                                                                                                                                   <  2:1368760/150‑1 (MQ=255)
     cAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCataa                                                                                                                                                  >  2:2020396/1‑150 (MQ=255)
            ttGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAaaataaat                                                                                                                                           <  2:3073659/150‑1 (MQ=255)
               aGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTa                                                                                                                                        <  2:3423294/150‑1 (MQ=255)
                gTTTGCGTGCTGTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTaa                                                                                                                                       <  1:5320118/150‑1 (MQ=255)
                  ttGCGTGCTTTTGGAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTaaaa                                                                                                                                     <  1:293886/150‑1 (MQ=255)
                       tGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGTTTTCTGCGTTTCTTCGCGAAATAATTCCGCATCGCAAATTATCCAAAAAATAAAATTAAAAAAACa                                                                                                                                >  1:4302379/1‑150 (MQ=38)
                          ttttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTGATAGCGCATTAAATACCCTTAGCAAATTATACATAAAAAAAATTTAAAATAaaaaaa                                                                                                                             >  1:1017315/1‑150 (MQ=37)
                          ttttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTATTCGCGAATTAATTACGCTTCGCAATTTATCCATAAAAAAAATTTAAAATAACaaaa                                                                                                                             >  1:4854053/1‑150 (MQ=255)
                          ttttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTATTAGCGAATTAATTACGCGTCGCAATTTATCCAAAAAAAAAATTTAAAATAACaaaa                                                                                                                             >  1:3016331/1‑150 (MQ=38)
                            ttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGCATTAATTCCGCTTCGCAATTTATCCATAAAATAAAATTAAAATAACAAAACa                                                                                                                           >  1:3334633/1‑150 (MQ=255)
                            ttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTATTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAaaaaaaaa                                                                                                                           >  1:1323678/1‑148 (MQ=255)
                             tGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGAAAAAAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACAt                                                                                                                          <  1:4498553/150‑1 (MQ=255)
                              gaaGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGAGTTCAGCGGCGAGTTTTAGTCTTTTATTAGCCAAAAAAAAAACCTACACAATTTATACAAAAAAAAAAATTaaaaaaaaaaaaaaaa                                                                                                                         >  1:4708049/3‑146 (MQ=16)
                               tAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATaa                                                                                                                        <  2:2358138/150‑1 (MQ=255)
                               tAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTACGCGTCGCAAATTATACAAAAAAAAAATTTaaaaaaaaaaaaaaaaa                                                                                                                        >  1:25123/1‑145 (MQ=38)
                                 ggCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAAtt                                                                                                                      >  2:4988873/1‑150 (MQ=255)
                                 ggCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAAAATAAtt                                                                                                                      >  1:3518451/1‑150 (MQ=255)
                                  gCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATta                                                                                                                     >  2:4940465/1‑150 (MQ=255)
                                  gCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTATTAGCGAATTAATTACGCTTAGCAATTTATACATAAAATAAATTTAAAATAACAAAACATAATta                                                                                                                     >  2:1701465/1‑150 (MQ=255)
                                     ggATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATtaaat                                                                                                                  <  1:4032031/150‑1 (MQ=255)
                                     ggATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTATTGGCGAAATAAATACGCGTAGCAAATTAACCAAAAAATAAAATTAAAAAAAAAAAACAAAAAtaaaa                                                                                                                  >  1:538708/1‑149 (MQ=255)
                                         aaGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATaaaa                                                                                                              <  2:935868/150‑1 (MQ=255)
                                         aaGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATaaaa                                                                                                              <  1:1515929/150‑1 (MQ=255)
                                          aGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAAt                                                                                                             <  2:770829/150‑1 (MQ=255)
                                          aGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTACGCTTCGCAATTTATCCAAAAAATAAATTTAAAATAACAAAACATAATTAAATAAAAt                                                                                                             >  2:217142/1‑150 (MQ=255)
                                           ggCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGGTTTTATTAGCGAAATAATTACGCTTAGCAATTTATACATAAAAAAAATATAAAATAAAAAAAAAAAAATaaaaaaaaag                                                                                                            >  1:5922160/1‑148 (MQ=255)
                                           ggCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGTGTTCTGCTTTTCGTCGCGCATTAATTCCCCATCGCAAATTAACCAAAAAAAAAAATTAAAAAAACAAAACAAAAGAaaaaaaaaag                                                                                                            >  1:4172824/1‑148 (MQ=255)
                                            gCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTAGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAAATTAAAAAAACAAAACATAAATAAATAAAAttt                                                                                                           >  2:3856476/1‑148 (MQ=255)
                                            gCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGTTTTGTGCGTTTATTCGCGAATTAATTACGCGTCGCAAATTATACAAAAAAAAAATTTAAAAAAACAAAAAAAAAATaaaaaaaaatt                                                                                                           >  1:5624392/1‑147 (MQ=255)
                                            gCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGTTTTCTGCTTTTCTTCGCGAAATAAATCCGCGTCGCAAAGTATCCATAAAATAAAGTTAAAATAAAAAAACaaaaaaaaaaaaaaatg                                                                                                           >  1:4778844/1‑147 (MQ=255)
                                            gCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGTTTTATGCTTTTATTAGCGAATTAATTCCGCTTCGCAATTTATACATAAAATAAATTTAAAATAAAAAAAAAAAATTAAATAAAAttt                                                                                                           >  2:1554641/1‑148 (MQ=255)
                                              gTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAAAAAAATTAAAATAACAAAAAATAAATAAATAAAATTTaa                                                                                                         >  2:1292491/1‑150 (MQ=255)
                                              gTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCGTCGCAATTTATACATAAAATAAATTTAAAATAACAAAACATAAATAAATAAAATGTaa                                                                                                         >  2:1121278/1‑150 (MQ=255)
                                               ttCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAAc                                                                                                        >  2:2530706/1‑150 (MQ=255)
                                               ttCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAAAAAATTTAAAATAACAAAACATAAATAAATAAAATTTaaa                                                                                                        >  2:5805929/1‑149 (MQ=255)
                                                tCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAAcc                                                                                                       >  2:4438436/1‑150 (MQ=255)
                                                tCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTCTTCGCGAATTAAATACGCTTGGCAATTTATACATAAAATAAATTTAAAATAAAAAAAAATAATTAAATAAAATTTaaac                                                                                                       >  1:3523622/1‑148 (MQ=255)
                                                  aCGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAAAAAAAAATAATTAAATAAAATTTAACCGc                                                                                                     >  2:5975262/1‑150 (MQ=255)
                                                   cgccgcATCCGGCAAGAAACAGCAAACAATCCACAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCt                                                                                                    <  2:5089615/150‑1 (MQ=255)
                                                    gccgcATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTATTATTAGCCAAATAATAACGCTTCGCAAAATATACAAAAAAAAAATTTAAAAAAAAAAAAAAAAATTAAATAAAAATTAAACGCtt                                                                                                   >  2:736345/1‑150 (MQ=255)
                                                    gccgcATCCGGCAAGAAACAGCAAACAAACCAACACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCtt                                                                                                   <  2:4546598/150‑1 (MQ=255)
                                                         aTCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATc                                                                                              <  2:268585/150‑1 (MQ=255)
                                                            cGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTATTCGCGAAATAATTACGCTTAGCAATTTATACATAAAATAAATTTAAAATAAAAAAAAAAAATTAAAAAAAAATTAACCCCTTTCaaattt                                                                                           >  2:1172874/1‑145 (MQ=255)
                                                             ggCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTATTCGCGAATTAATTCCGCGTCGCAATTTTTACATAAAATAAAATTAAAATAACAAAACAAAAATAAATAAAATATAACCGCTTTCATATTGc                                                                                          >  2:4070141/1‑150 (MQ=255)
                                                             ggCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGGTTTGTGGTTTTGTTGGCGAATTAATTACGCGTCGCAAATTATGCATAAAATAAATTTAAAATAAAAAAAAAAAAATAAATAAAATTTTAACGCGTTGATATTgg                                                                                          >  1:5813203/1‑149 (MQ=255)
                                                              gCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCt                                                                                         <  2:1447800/150‑1 (MQ=255)
                                                              gCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCt                                                                                         <  2:5274479/150‑1 (MQ=255)
                                                                aaGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTTCTTTTATTAGCGAATTAATTCCCCTTAGCAAATTATACATAAAATAAAATTAAAAAAACAAAAAAAAAAAAAATAAAATTTAAACGCATTAATCTTTCTgg                                                                                       >  2:2164177/1‑150 (MQ=255)
                                                                  gAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGaa                                                                                     <  1:1870581/150‑1 (MQ=255)
                                                                   aaaCAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAACACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAAt                                                                                    <  2:1770241/150‑1 (MQ=255)
                                                                   aaaCAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAAt                                                                                    >  2:3498086/1‑150 (MQ=255)
                                                                    aaCAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAAtt                                                                                   <  2:1913926/150‑1 (MQ=255)
                                                                       aGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAACACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCa                                                                                <  2:5212830/150‑1 (MQ=255)
                                                                                tCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCGTTTCTTCGCCAATTAATTCCGCTTCGCAAATTATCCAAAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCGTTTAt                                                                       >  2:4706015/1‑150 (MQ=255)
                                                                                    ccaCGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAAGTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTAttctt                                                                   <  1:2623275/148‑1 (MQ=255)
                                                                                      aCGCCGCGTTCAGCGGCGTATTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATtcttct                                                                 <  1:2691959/150‑1 (MQ=255)
                                                                                         ccgcGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAACTCCGCTTCGCAATTTATACATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATTTAACCGCGTTCATCTTTCTGGAATTTAACGCTTTTATTCTTCTGCa                                                              >  2:1850434/1‑150 (MQ=255)
                                                                                         ccgcGTTCAGCGGCGTTTTTTCTGCTTTTATTAGCGAAATAATTCCGCATCGCAAATTATAAAAAAAATAAATTTAAAATAACAAAAAATAAATAAATAAAATTTAACCCCTTTCATAATGCTGGAATTTCAAGCGTTTATTCTTCTgaa                                                              >  2:5572710/1‑148 (MQ=255)
                                                                                          cgcgTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCaa                                                             <  2:242224/150‑1 (MQ=255)
                                                                                                cAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCttt                                                       <  1:2356287/150‑1 (MQ=255)
                                                                                                cAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCttt                                                       <  2:5495540/150‑1 (MQ=255)
                                                                                                 aGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTc                                                      <  2:650156/150‑1 (MQ=255)
                                                                                                 aGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTc                                                      <  2:5419006/150‑1 (MQ=255)
                                                                                                   cggcgTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCaa                                                    >  2:5855119/1‑150 (MQ=255)
                                                                                                     gcgTTTTTTCTGCTTTTCGTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAAcc                                                  <  2:3735533/150‑1 (MQ=255)
                                                                                                      cgTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTAATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTAAACCg                                                 >  2:5668693/1‑150 (MQ=255)
                                                                                                        ttttttCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTACATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCa                                               <  1:1840769/150‑1 (MQ=255)
                                                                                                          ttttCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCaaa                                             <  1:2236529/150‑1 (MQ=255)
                                                                                                            ttCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACt                                           >  2:5010955/1‑150 (MQ=255)
                                                                                                              cTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTa                                         <  1:3273339/150‑1 (MQ=255)
                                                                                                                  ttttCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGcc                                     >  2:4900924/1‑150 (MQ=255)
                                                                                                                   tttCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCt                                    >  1:712515/1‑150 (MQ=255)
                                                                                                                    ttcttcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCtt                                   <  1:853409/150‑1 (MQ=255)
                                                                                                                     tcttcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTg                                  <  2:425610/150‑1 (MQ=255)
                                                                                                                      cttcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGt                                 <  2:3362243/150‑1 (MQ=255)
                                                                                                                      cttcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGt                                 <  1:4211953/150‑1 (MQ=255)
                                                                                                                            gAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACaaa                           >  1:5157504/1‑150 (MQ=255)
                                                                                                                             aattaattCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACaaaa                          >  2:3334074/1‑150 (MQ=255)
                                                                                                                              attaattCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACaaaaa                         <  2:4317319/150‑1 (MQ=255)
                                                                                                                               ttaattCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAat                        <  1:5010955/150‑1 (MQ=255)
                                                                                                                                taattCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAatc                       <  1:3092400/150‑1 (MQ=255)
                                                                                                                                 aattCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAatca                      <  2:1627512/150‑1 (MQ=255)
                                                                                                                                 aattCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAatca                      <  2:2069409/150‑1 (MQ=255)
                                                                                                                                  attCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAatcat                     >  1:779015/1‑150 (MQ=255)
                                                                                                                                   ttCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAatcatc                    <  1:5975262/150‑1 (MQ=255)
                                                                                                                                    tCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAAtcatca                   <  2:5157504/150‑1 (MQ=255)
                                                                                                                                     ccGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCaa                  >  1:3741978/1‑150 (MQ=255)
                                                                                                                                      cgcgacGCAATTTATACATAAAATAAATTTAAAATAACAAAACATAATTAACTAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCaaa                 <  1:16586/145‑1 (MQ=255)
                                                                                                                                      cGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCaaa                 >  2:2311973/1‑150 (MQ=255)
                                                                                                                                      cGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCaaa                 >  1:1381130/1‑150 (MQ=255)
                                                                                                                                          tCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAatat             <  2:2831510/150‑1 (MQ=255)
                                                                                                                                             cAATTTATACATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATAtgtg          <  1:33691/150‑1 (MQ=255)
                                                                                                                                                tttATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTCGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCgg       <  1:519337/150‑1 (MQ=255)
                                                                                                                                                  tATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGGtt     <  2:4854053/150‑1 (MQ=255)
                                                                                                                                                    tCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGGTTGc   <  1:1172874/150‑1 (MQ=255)
                                                                                                                                                     ccATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGGTTGCt  <  2:2966848/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGGTTGCT  >  NC_000913/707871‑708169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: