Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 756,150 C→T 45.9% Q82* (CAG→TAG)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,1500CT45.9% 122.1 / inf 418Q82* (CAG→TAG) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base C (116/110);  new base T (90/102);  total (206/212)
Fisher's exact test for biased strand distribution p-value = 3.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 425 total aligned reads displayed.

CTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCG  >  NC_000913/756002‑756299
                                                                                                                                                    |                                                                                                                                                     
 tgtgCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAg                                                                                                                                                     >  2:862804/1‑150 (MQ=255)
 tgtgCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAg                                                                                                                                                     >  1:4915173/1‑150 (MQ=255)
 tgtgCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAg                                                                                                                                                     <  2:235348/150‑1 (MQ=255)
     cgcTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGAcg                                                                                                                                                 <  2:5475383/150‑1 (MQ=255)
        tgctCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGa                                                                                                                                              <  1:1315231/150‑1 (MQ=255)
        tgctCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGa                                                                                                                                              <  1:3339120/150‑1 (MQ=255)
         gctCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGAt                                                                                                                                             <  2:2137859/150‑1 (MQ=255)
               tAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAata                                                                                                                                       >  1:4336014/1‑150 (MQ=255)
                aaaGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAatat                                                                                                                                      <  2:2961701/150‑1 (MQ=255)
                 aaGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAatata                                                                                                                                     <  2:5282999/150‑1 (MQ=255)
                 aaGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAatata                                                                                                                                     <  2:2689075/150‑1 (MQ=255)
                  aGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAatatat                                                                                                                                    <  1:5903974/150‑1 (MQ=255)
                    tCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATGACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATAtgt                                                                                                                                  <  1:3088894/150‑1 (MQ=255)
                        cccGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTaa                                                                                                                              >  1:5729233/1‑150 (MQ=255)
                          cGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTaaaa                                                                                                                            >  1:120391/1‑150 (MQ=255)
                          cGACCCGGTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTaaaa                                                                                                                            >  1:2038230/1‑150 (MQ=255)
                           gACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAAc                                                                                                                           >  1:5352941/1‑150 (MQ=255)
                              ccGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCgg                                                                                                                        <  2:1493221/150‑1 (MQ=255)
                               cGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCggg                                                                                                                       <  1:5761284/150‑1 (MQ=255)
                                gTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGc                                                                                                                      >  1:2884786/1‑150 (MQ=255)
                                gTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGc                                                                                                                      <  2:1062281/150‑1 (MQ=255)
                                   cccATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCgg                                                                                                                   <  1:4084965/150‑1 (MQ=255)
                                     cATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGaa                                                                                                                 <  2:2589917/150‑1 (MQ=255)
                                       tACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGc                                                                                                               >  2:4206800/1‑150 (MQ=255)
                                       tACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGc                                                                                                               >  1:3953119/1‑150 (MQ=255)
                                          cGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGAt                                                                                                            >  2:209867/1‑150 (MQ=255)
                                          cGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGAt                                                                                                            >  1:2605133/1‑150 (MQ=255)
                                           gTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGAtt                                                                                                           <  1:4417866/150‑1 (MQ=255)
                                            tttCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTc                                                                                                          >  1:5998241/1‑150 (MQ=255)
                                             ttCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCt                                                                                                         <  2:2486849/150‑1 (MQ=255)
                                                agcgcAAGGCGGCATTACCGTTGCGCTGGGTAATAACCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGa                                                                                                      >  1:967484/2‑150 (MQ=255)
                                                  cgcAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAAc                                                                                                    >  2:3272323/1‑150 (MQ=255)
                                                  cccAAGGCGGCATTACCGTTGCGCTGGTTAATACCAATTAATATAAATGGGAATGGAATATGTACGACACCGTGAAAGGGTCGGAATATATCGGTGACCAGTACGCTATTTAATATATGTGAAAAACCGGGCCGGAAGCGATTCTGGAAc                                                                                                    <  1:1136708/148‑1 (MQ=255)
                                                   gcAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACt                                                                                                   >  2:5578516/1‑150 (MQ=255)
                                                        gcggcATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAac                                                                                              >  2:5120590/1‑150 (MQ=255)
                                                             aTTACCGTTGCGCTGGTTAATACCAATGAAGATAAATGTTAATGCCATATGTACGACACCTTTAAAGGGACGGACTATATCGGTGACCAGGACGCTATTTAATATATTTGTAAAACCGGGCCGCAAGCGATTCTGGAACTCGAACACATg                                                                                         <  1:2518075/150‑1 (MQ=255)
                                                             aTTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATg                                                                                         >  2:2890320/1‑150 (MQ=255)
                                                                  cGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCt                                                                                    >  2:764803/1‑150 (MQ=255)
                                                                   gTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTTTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTg                                                                                   <  1:702751/150‑1 (MQ=255)
                                                                    ttGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGc                                                                                  >  2:498197/1‑150 (MQ=255)
                                                                        gcTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGtt                                                                              <  1:1600353/150‑1 (MQ=255)
                                                                        gcTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGtt                                                                              <  2:388969/150‑1 (MQ=255)
                                                                         cTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTtc                                                                             <  1:187739/150‑1 (MQ=255)
                                                                          tGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTtct                                                                            >  1:3216393/1‑150 (MQ=255)
                                                                           gggTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTtctc                                                                           <  1:2137400/150‑1 (MQ=255)
                                                                             gTAATACCCATGAAGATACCTGGGAATGGCATATGTACGACACCGTGAACGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTcgc                                                                         <  1:1025363/150‑1 (MQ=255)
                                                                             gTAATACCAATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTcgc                                                                         <  1:5080978/150‑1 (MQ=255)
                                                                              tAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTcgcg                                                                        <  2:5733966/150‑1 (MQ=255)
                                                                               aaTACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGt                                                                       <  2:4852850/150‑1 (MQ=255)
                                                                                    ccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCga                                                                  >  2:3169267/1‑150 (MQ=255)
                                                                                    ccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCga                                                                  >  2:3461834/1‑150 (MQ=255)
                                                                                        gAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCgatgat                                                              <  2:6005531/150‑1 (MQ=255)
                                                                                         aaGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGatgatg                                                             <  1:373651/150‑1 (MQ=255)
                                                                                         aaGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGTGCCTGCCGTTCTCGCGTCTCgatgatt                                                             <  2:1823622/150‑2 (MQ=255)
                                                                                           gATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGt                                                           <  2:4174846/150‑1 (MQ=255)
                                                                                              aaCTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGt                                                        <  2:5646695/150‑1 (MQ=255)
                                                                                               aCTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGta                                                       >  2:5390353/1‑150 (MQ=255)
                                                                                               aCTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGta                                                       >  1:264716/1‑150 (MQ=255)
                                                                                               aCTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACCATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGta                                                       >  2:439680/1‑150 (MQ=255)
                                                                                                cTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtat                                                      <  2:3524315/150‑1 (MQ=255)
                                                                                                 tGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatc                                                     >  1:3787871/1‑150 (MQ=255)
                                                                                                 tGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatc                                                     >  1:3504829/1‑150 (MQ=255)
                                                                                                 tGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatc                                                     >  1:3880641/1‑150 (MQ=255)
                                                                                                    gAATGTCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatctat                                                  <  2:4738135/150‑1 (MQ=255)
                                                                                                     aaTGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatctatc                                                 >  2:1684213/1‑150 (MQ=255)
                                                                                                        ggCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAAc                                              <  1:4035602/150‑1 (MQ=255)
                                                                                                         gCATATGTATGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACg                                             >  2:4539044/1‑150 (MQ=255)
                                                                                                           atatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTc                                           >  2:5453024/1‑150 (MQ=255)
                                                                                                               gTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGtt                                       <  2:1167588/150‑1 (MQ=255)
                                                                                                                tACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGttt                                      >  1:2279269/1‑150 (MQ=255)
                                                                                                                  cGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTgg                                    >  2:4073305/1‑150 (MQ=255)
                                                                                                                   gACACCGTGAATGGGTCGGACTATATGGGTGAGTAGGACGCGATTGAATATATGTGTAAAACGGGGCCGGACGCGATTCTGGAACTCGAACACTTGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGc                                   <  1:554721/150‑1 (MQ=255)
                                                                                                                   gACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGc                                   <  1:3660466/150‑1 (MQ=255)
                                                                                                                    acacCGTGAAAGGGTCGTACTATATCGGTGACTAGGACGCGATTTAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCg                                  <  1:804348/150‑1 (MQ=255)
                                                                                                                     cacCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTTAATATTTGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCgg                                 <  1:1595131/150‑1 (MQ=255)
                                                                                                                         gTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAg                             >  1:4376159/1‑150 (MQ=255)
                                                                                                                         gTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAg                             >  1:296446/1‑150 (MQ=255)
                                                                                                                          tGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGt                            >  2:459564/1‑150 (MQ=255)
                                                                                                                           gAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTc                           >  2:3753877/1‑150 (MQ=255)
                                                                                                                             aaGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGa                         >  2:1122735/1‑150 (MQ=255)
                                                                                                                              aGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGaa                        >  1:2781229/1‑150 (MQ=255)
                                                                                                                               gggTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGaaa                       <  1:5656218/150‑1 (MQ=255)
                                                                                                                               gggTCGGAATATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGaaa                       <  2:2554318/150‑1 (MQ=255)
                                                                                                                                 gTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGaaaaa                     <  1:5412746/150‑1 (MQ=255)
                                                                                                                                  tCGGACTATATCTGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAAc                    >  1:3547857/1‑150 (MQ=255)
                                                                                                                                  tCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAAc                    <  1:4733068/150‑1 (MQ=255)
                                                                                                                                    ggACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACtt                  <  1:209867/150‑1 (MQ=255)
                                                                                                                                     gACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTc                 >  2:5792453/1‑150 (MQ=255)
                                                                                                                                      aCTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcg                <  2:1262699/150‑1 (MQ=255)
                                                                                                                                        tataTCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggc              >  1:4316788/1‑150 (MQ=255)
                                                                                                                                            tCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGgcggcg          >  1:1426764/1‑150 (MQ=255)
                                                                                                                                             cGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGa         <  1:51780/150‑1 (MQ=255)
                                                                                                                                             cGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGa         <  2:5159237/150‑1 (MQ=255)
                                                                                                                                             cGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGa         >  2:5878744/1‑150 (MQ=255)
                                                                                                                                              ggTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAg        <  2:3974437/150‑1 (MQ=255)
                                                                                                                                                tGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCa      >  1:4207457/1‑150 (MQ=255)
                                                                                                                                                  aCCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAgg    <  2:2038230/150‑1 (MQ=255)
                                                                                                                                                    cAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAggcg  >  1:4989940/1‑150 (MQ=255)
                                                                                                                                                    cAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAggcg  <  1:4172562/150‑1 (MQ=255)
                                                                                                                                                    cAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAggcg  >  2:1896671/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCG  >  NC_000913/756002‑756299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: