Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,015,912 T→A 6.2% intergenic (+30/+40) rmf → / ← fabA ribosome modulation factor/beta‑hydroxydecanoyl thioester dehydrase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,015,9120TA 6.2% 1270.8 / 53.1 386intergenic (+30/+40)rmf/fabAribosome modulation factor/beta‑hydroxydecanoyl thioester dehydrase
Reads supporting (aligned to +/- strand):  ref base T (175/183);  new base A (13/11);  total (188/198)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.70e-01

Only 100 of 390 total aligned reads displayed.

ATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGAC  >  NC_000913/1015764‑1016061
                                                                                                                                                    |                                                                                                                                                     
aTCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCAtt                                                                                                                                                      >  1:5595536/1‑150 (MQ=255)
aTCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCAtt                                                                                                                                                      >  1:2989459/1‑150 (MQ=255)
aTCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCAtt                                                                                                                                                      >  1:4357485/1‑150 (MQ=255)
    ggCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCgg                                                                                                                                                  >  2:3729032/1‑150 (MQ=255)
     gCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGa                                                                                                                                                 <  2:4010276/150‑1 (MQ=255)
       cGGCATCGCCGGACGCTCAAAACAATTGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCTTGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAgg                                                                                                                                               <  1:5321850/150‑1 (MQ=255)
        ggCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCAATGCGGAGGt                                                                                                                                              <  2:1312815/150‑1 (MQ=255)
        ggCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCAATGCGGAGGt                                                                                                                                              <  2:2332256/150‑1 (MQ=255)
        ggCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCAATGCGGAGGt                                                                                                                                              <  2:377680/150‑1 (MQ=255)
        ggCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCAATGCGGAGGt                                                                                                                                              <  2:5493212/150‑1 (MQ=255)
        ggCATCGCCGGACGCACAAAACAAATGTGTCCCTATCAGACGCTGAAACAAAGCTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCAATGCGGAGGt                                                                                                                                              <  1:4916874/150‑1 (MQ=255)
          cATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGttt                                                                                                                                            >  2:1031077/1‑150 (MQ=255)
           aTCGCCGGACTCTCAAAAGAAATGTGTCCATATCAGACGCTGAATCAATGGTAACATTGGCTGGGTGGCTGGCTAGATGCCATGGCGGACAGGGTAGTAATGGCCTGTTTCTTTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTc                                                                                                                                           <  1:3502085/150‑1 (MQ=255)
            tCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCg                                                                                                                                          <  2:4292383/150‑1 (MQ=255)
             cGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCAATGCGGAGGTTTctt                                                                                                                                         <  1:1431860/150‑3 (MQ=255)
             cGCCGGACGCACACACGACCTGGGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGCTGCCTTGGGGGTGTGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCAGTGCGGAGGTTTCGc                                                                                                                                         <  2:5238785/150‑1 (MQ=255)
               ccGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCt                                                                                                                                       >  2:5296025/1‑150 (MQ=255)
                 ggACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCttt                                                                                                                                     <  1:3181272/150‑1 (MQ=255)
                 ggACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAAGGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCttt                                                                                                                                     <  1:4389918/150‑1 (MQ=255)
                  gACGCTCAACAGAAATGTGTCCCTAACAGACGCTGACTCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCtttt                                                                                                                                    <  2:3332880/150‑1 (MQ=255)
                   aCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTg                                                                                                                                   <  1:3268803/150‑1 (MQ=255)
                   aCGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTg                                                                                                                                   <  2:5102335/150‑1 (MQ=255)
                         aaaaGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATActc                                                                                                                             >  1:3668396/1‑150 (MQ=255)
                           aaGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACtctg                                                                                                                           >  1:5527702/1‑150 (MQ=255)
                           aaGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACtctg                                                                                                                           >  1:1994222/1‑150 (MQ=255)
                            aGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACTATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACtctgt                                                                                                                          <  2:5415054/150‑1 (MQ=255)
                              aaaTGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACtctgtct                                                                                                                        >  2:1572096/1‑150 (MQ=255)
                                aTGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGa                                                                                                                      <  2:4075275/150‑1 (MQ=255)
                                aTGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGa                                                                                                                      <  2:4841512/150‑1 (MQ=255)
                                aTGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGa                                                                                                                      <  1:1678008/150‑1 (MQ=255)
                                 tgtgACCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCGGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGAt                                                                                                                     <  1:2511168/150‑1 (MQ=255)
                                     tCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATtata                                                                                                                 >  2:5690619/1‑150 (MQ=255)
                                      cccTAACAGACGGTGAATCACAGGTCTCATTGGGTGGGTGGGTGGCGTGTAGCCTTGGAGGACTGGGTAGTTATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCAGCTTTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATaa                                                                                                                <  1:3063920/150‑1 (MQ=255)
                                        cTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATc                                                                                                              <  1:1008333/150‑1 (MQ=255)
                                         taacAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGCCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGACTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGAGTATAATCa                                                                                                             >  2:3610284/4‑150 (MQ=255)
                                         tATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCa                                                                                                             >  1:2119462/1‑150 (MQ=255)
                                           tCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGa                                                                                                           >  1:499290/1‑150 (MQ=255)
                                           tCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGa                                                                                                           <  1:612635/150‑1 (MQ=255)
                                           tCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGa                                                                                                           <  1:3715982/150‑1 (MQ=255)
                                           tCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGa                                                                                                           >  2:938473/1‑150 (MQ=255)
                                            cAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGaa                                                                                                          <  2:3858940/150‑1 (MQ=255)
                                             aGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAg                                                                                                         >  1:713728/1‑150 (MQ=255)
                                                cGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGacagag                                                                                                      <  2:2507440/150‑6 (MQ=255)
                                                 gCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAg                                                                                                     <  1:3450688/150‑1 (MQ=255)
                                                   tGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTACTGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTGTGATTCTCTGTCTGATTATAATCGGACGGCAGAc                                                                                                   >  2:5960512/1‑150 (MQ=255)
                                                   tGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGAc                                                                                                   <  2:4997306/150‑1 (MQ=255)
                                                       tCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTAt                                                                                               >  1:2292905/1‑150 (MQ=255)
                                                         aaaGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATcc                                                                                             >  1:259807/1‑150 (MQ=255)
                                                         aaaGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATcc                                                                                             >  2:1364832/1‑150 (MQ=255)
                                                          aaGGTCACACTGGCTGGGAGGCGGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCt                                                                                            <  1:2392838/150‑1 (MQ=255)
                                                           aGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTACTGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTg                                                                                           <  1:1045292/150‑1 (MQ=255)
                                                                acaATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACa                                                                                      >  1:2812782/1‑150 (MQ=255)
                                                                        tGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACt                                                                              <  1:3774154/150‑1 (MQ=255)
                                                                        tGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACt                                                                              >  1:5239926/1‑150 (MQ=255)
                                                                         gggAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACtt                                                                             >  1:4497185/1‑150 (MQ=255)
                                                                         gggAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACtt                                                                             >  1:5488931/1‑150 (MQ=255)
                                                                          ggAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACttt                                                                            >  2:2561529/1‑150 (MQ=255)
                                                                          ggAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACttt                                                                            <  2:4739080/150‑1 (MQ=255)
                                                                                tGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTc                                                                      >  1:4718741/1‑150 (MQ=255)
                                                                                tGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTc                                                                      >  1:5097856/1‑150 (MQ=255)
                                                                                tGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTc                                                                      >  1:4082219/1‑150 (MQ=255)
                                                                                tGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTCGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTc                                                                      <  1:1144375/150‑1 (MQ=255)
                                                                                       aaGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCg                                                               <  2:3215817/150‑1 (MQ=255)
                                                                                           cATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGtat                                                           >  1:846848/1‑150 (MQ=255)
                                                                                           cATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGtat                                                           >  2:5623709/1‑150 (MQ=255)
                                                                                            aTGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGtata                                                          <  1:1880235/150‑1 (MQ=255)
                                                                                             tGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAg                                                         <  1:3804351/150‑1 (MQ=255)
                                                                                              ggCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGa                                                        >  1:2247010/1‑150 (MQ=255)
                                                                                               gCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGAt                                                       <  2:2294141/150‑1 (MQ=255)
                                                                                               gCGGACAGGGTAGTAAGGGCCTGATTCTGTCTCTTTAACAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCGGTCTGATTATACTCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGAt                                                       <  2:1071272/150‑1 (MQ=255)
                                                                                                       ggTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAgacgac                                               >  1:396709/1‑150 (MQ=255)
                                                                                                        gtagtaATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGAcc                                              <  2:1711977/150‑1 (MQ=255)
                                                                                                              aTGGCCTGATTCTGTCTCTTTAAATAGAAACCTCCGCATTGCGGAGGTTTCGGCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACGGAGTTTCAGGTCGCTGGCGGTATAGTTCAGACGACCATcttc                                        >  2:3908117/1‑147 (MQ=255)
                                                                                                                 gCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAg                                     <  2:5882199/150‑1 (MQ=255)
                                                                                                                 gCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAg                                     >  2:1169574/1‑150 (MQ=255)
                                                                                                                  ccTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGc                                    >  2:4527627/1‑150 (MQ=255)
                                                                                                                  ccTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGc                                    >  2:3389822/1‑150 (MQ=255)
                                                                                                                   cTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCa                                   >  2:1934533/1‑150 (MQ=255)
                                                                                                                       ttCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTc                               <  2:2999236/150‑1 (MQ=255)
                                                                                                                       ttCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTc                               <  1:5270937/150‑1 (MQ=255)
                                                                                                                          tGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGcc                            >  2:1843398/1‑150 (MQ=255)
                                                                                                                            tctcTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCAt                          <  1:4563396/150‑1 (MQ=255)
                                                                                                                                 ttAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGcc                     >  2:866107/1‑150 (MQ=255)
                                                                                                                                  tAAAAAGAAACCTCCGCATGGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCa                    <  1:1031077/150‑1 (MQ=255)
                                                                                                                                     ggcgAAACCTCCGCAATGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCGGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGc                 >  2:239665/4‑150 (MQ=255)
                                                                                                                                     aaaGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGc                 >  2:1515423/1‑150 (MQ=255)
                                                                                                                                       aGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCTCCATCCGCCAGGccc               >  1:2345944/1‑150 (MQ=255)
                                                                                                                                         aaaCCTCCGCAATGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCAt             >  2:2744804/1‑150 (MQ=255)
                                                                                                                                         aaaCCTCCGCAATGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCAt             >  2:1637515/1‑150 (MQ=255)
                                                                                                                                           aCCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCCTCCGCCAGGCCCaaaa           >  1:4430768/1‑150 (MQ=255)
                                                                                                                                           aCCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATaa           >  1:5798155/1‑150 (MQ=255)
                                                                                                                                           aCCTCCGCAATGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATaa           >  1:5666794/1‑150 (MQ=255)
                                                                                                                                              tCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCa        >  2:2845562/1‑150 (MQ=255)
                                                                                                                                              tCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCa        >  2:3107550/1‑150 (MQ=255)
                                                                                                                                              tCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCa        >  2:1801444/1‑150 (MQ=255)
                                                                                                                                              tCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCa        >  2:1435748/1‑150 (MQ=255)
                                                                                                                                              tCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCa        >  1:5457029/1‑150 (MQ=255)
                                                                                                                                              tCCGCATTGCGGAGGTTTCGCCTTTTGATAATCTGTCTGATTATATTCAGAAGGCAGACGTATCCTGGAACAGTCCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAGCCAGCACTTCGCCATCCGCCAGGCCCCTAATCa        >  1:1283569/1‑150 (MQ=255)
                                                                                                                                               ccGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAg       >  1:5041783/1‑150 (MQ=255)
                                                                                                                                                cGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAga      <  1:1233925/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATCAGGCCGGCATCGCCGGACGCTCAAAAGAAATGTGTCCCTATCAGACGCTGAATCAAAGGTCACAATGGCTGGGAGGCTGGCGAGAAGCCATGGCGGACAGGGTAGTAATGGCCTGATTCTGTCTCTTTAAAAAGAAACCTCCGCATTGCGGAGGTTTCGCCTTTTGATACTCTGTCTGATTATAATCAGAAGGCAGACGTATCCTGGAACAGACCGACTTTCAGGTCGCTGGCGGTATAGATCAGACGACCATCAACCAGCACTTCGCCATCCGCCAGGCCCATAATCAGACGAC  >  NC_000913/1015764‑1016061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: