Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,351,227 A→C 5.7% intergenic (‑188/+180) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,351,2270AC 5.7% 904.9 / 8.0 294intergenic (‑188/+180)fabI/ycjDenoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
Reads supporting (aligned to +/- strand):  ref base A (118/149);  new base C (21/2);  total (140/154)
Fisher's exact test for biased strand distribution p-value = 1.04e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 302 total aligned reads displayed.

ATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAG  >  NC_000913/1351079‑1351376
                                                                                                                                                    |                                                                                                                                                      
         ttCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAg                                                                                                                                              <  1:2900133/150‑1 (MQ=255)
         ttCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAg                                                                                                                                              <  1:2713298/150‑1 (MQ=255)
          tcgtcgTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTGCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAgg                                                                                                                                             <  1:494312/150‑1 (MQ=255)
             tcgtTTTAGTAAACAGTACGAACAGCTAAACGGTTATTATACTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGa                                                                                                                                          <  2:2662455/150‑1 (MQ=255)
              cgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGAc                                                                                                                                         <  1:3216660/150‑1 (MQ=255)
                ttttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCg                                                                                                                                       >  2:5691374/1‑150 (MQ=255)
                 tttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTGCCCTCACCCTAACCCTCTCCCCAGTGGGGGGAGGGGTCCGt                                                                                                                                      >  2:4791098/1‑150 (MQ=255)
                  ttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa                                                                                                                                     <  1:3408603/150‑1 (MQ=255)
                    aGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAtt                                                                                                                                   <  1:5291293/150‑1 (MQ=255)
                    aGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCAACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAtt                                                                                                                                   <  2:2043481/150‑1 (MQ=255)
                      tAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgt                                                                                                                                 <  2:1319911/150‑1 (MQ=255)
                      tAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgt                                                                                                                                 >  2:2933217/1‑150 (MQ=255)
                      tAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgt                                                                                                                                 <  1:2613215/150‑1 (MQ=255)
                       aaaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgtg                                                                                                                                <  2:5479725/150‑1 (MQ=255)
                       aaaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgtg                                                                                                                                <  1:4087589/150‑1 (MQ=255)
                         aCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCa                                                                                                                              <  2:1792462/150‑1 (MQ=255)
                         aCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCa                                                                                                                              <  1:1688544/150‑1 (MQ=255)
                         aCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCa                                                                                                                              <  1:1115778/150‑1 (MQ=255)
                         aCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAAACCAATCCCCAGAGGGGCGAGGGGCCCGTATTGTGCa                                                                                                                              >  2:1885020/1‑150 (MQ=255)
                          cAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCCGAGGGGCGAGGGGACCGTATTGGGCaa                                                                                                                             >  2:3806615/1‑150 (MQ=255)
                          cAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGcca                                                                                                                             >  1:1414161/1‑148 (MQ=255)
                               cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATAtt                                                                                                                       <  1:5509204/150‑1 (MQ=255)
                               cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCGTTTCCCCGCACCCAAACCCTCTCCCCAGAGGGGCGAGGGGCCCGTATTGTGCAAAAAtt                                                                                                                       >  1:4321651/1‑150 (MQ=255)
                                gAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAACCCTCTCCCCAGAGGGGCGAGGGGACCGGATTGTGCAAAAATTg                                                                                                                      >  2:3230642/1‑150 (MQ=255)
                                gAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCAAACCCACTCCCCACAGGGGCGAGGGGACCCTATTGTGCAAataatt                                                                                                                       >  2:323837/1‑147 (MQ=255)
                                    aGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAc                                                                                                                  <  1:5913371/150‑1 (MQ=255)
                                        aaaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCa                                                                                                              <  1:1241950/150‑1 (MQ=255)
                                        aaaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAAACCACACCCCAGAGGGGCGAGGGGACCGTATTGTGCAAAAAATTTTAACCCa                                                                                                              >  1:1343703/1‑150 (MQ=255)
                                        aaaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAACCCACTACCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTTTTAccccc                                                                                                              >  1:832745/1‑149 (MQ=255)
                                         aaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCCCCACCAAAACCAAAACCCACAGGGGCGCGGGGGCCCGATTTTGGAAAAATATTTTAcccac                                                                                                              >  1:2092643/1‑148 (MQ=255)
                                          aCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCACACCCAAACCCACTACCCAGCGGGGCGAGGGGCCCGTATTGTGGCAAAATTGTTACCCCAGc                                                                                                            >  1:4735800/1‑150 (MQ=255)
                                            ggTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGcaa                                                                                                          >  2:5945193/1‑150 (MQ=255)
                                                attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                                                                                      <  1:2012545/150‑1 (MQ=255)
                                                attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                                                                                      <  2:15809/150‑1 (MQ=255)
                                                 ttatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaac                                                                                                     <  1:657134/150‑1 (MQ=255)
                                                 ttatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAGTGTGCAAATATTGTTACCCCAGCaacaaac                                                                                                     >  2:4475988/1‑150 (MQ=255)
                                                 ttatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAAACCTCACCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATAATTTTAACCCCGCaacaaac                                                                                                     >  2:3586672/1‑150 (MQ=39)
                                                   ataATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAg                                                                                                   <  1:3047594/150‑1 (MQ=255)
                                                    taATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAgg                                                                                                  <  1:3739933/150‑1 (MQ=255)
                                                     aaTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGc                                                                                                 >  2:3388701/1‑150 (MQ=255)
                                                       tCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCACCGCACCCGAACCCACGCCCCCGGGGGGGGCGGGGGCCGGATTGTGGCAAAATTGGTTCCCCAGCACCCACCACGCTc                                                                                               >  2:2284686/1‑150 (MQ=255)
                                                       tCAACCTGGCTGTGAGTAGATATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTc                                                                                               <  1:4825111/150‑1 (MQ=255)
                                                         aaCCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCAt                                                                                             <  2:1876177/150‑1 (MQ=255)
                                                          aCCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATa                                                                                            <  2:2465219/150‑1 (MQ=255)
                                                            cTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACa                                                                                          <  1:5177713/150‑1 (MQ=255)
                                                            cTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAAACCAAACCCCAAAGGGGCGAGGGGACCCAAATTTGCAAAAAATTTTAACCCAACAACAAAAAAGCTCATACa                                                                                          >  1:1462525/1‑150 (MQ=255)
                                                             tGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAg                                                                                         >  2:3502453/1‑150 (MQ=255)
                                                             tGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAAACCACAACCCCCACGGGCGAGGGGGCCCTAATGTGCAAAAAATGTTAACCCACCCACAAACACGCCCaaaaac                                                                                         >  1:1303187/1‑145 (MQ=255)
                                                              ggCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGc                                                                                        <  2:4626752/150‑1 (MQ=255)
                                                              ggCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGc                                                                                        <  1:935101/150‑1 (MQ=255)
                                                              ggCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCAGCCGCAGGGGGGGCGCATTTCCCCGCACCCAAACCCGCTCCCCCGCCGGGCGAGCGGACCGTCTTGTGCACATATTGTTACGCCAGCGACACACACGCGCATACAGc                                                                                        >  2:846428/1‑150 (MQ=255)
                                                               gCTGTGAGTAGCTATAGGTGCCAGGTCCGACCGGAGCTGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCCCATACAGcc                                                                                       <  1:1573648/150‑1 (MQ=255)
                                                                 tgtgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACACAAAACCACTCACCACAGGGGCCACGGGTCCCGAATGTTCAAAAAATTTTAAACCAGAAACAAAAACGCGCAAACAAcccc                                                                                     >  2:2820988/1‑150 (MQ=255)
                                                                 tgtgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCACACCCAAAACCACAACCCACAGGGGCGAGGGGACCCGAATTTGGAAAAATATTTAACCCACCAACAAAAAAGCTCAAAAAAcccc                                                                                     >  1:2449007/1‑150 (MQ=255)
                                                                   tgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTa                                                                                   <  1:4668421/150‑1 (MQ=255)
                                                                   tgAGTAGCTATAGGTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTa                                                                                   <  2:401518/150‑1 (MQ=255)
                                                                    gAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTaa                                                                                  <  1:2954380/150‑1 (MQ=255)
                                                                    gAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTaa                                                                                  <  1:4401694/150‑1 (MQ=255)
                                                                      gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAAACCACACCCCAGAGGGGCGAGGGGGCCCTATTGTGCCAAAAATGTTACCCCACCAACAAACAGGCTCAAACACCCCCAAAcc                                                                                >  1:5761171/1‑150 (MQ=255)
                                                                      gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCAAACCCAAAACCAAAACCCCCAGGGGCGCGGGGGCCCGATTTTTCAAATAATTTTAACCCACCAACAAAAAAGCGCAAAAAGCCCCCAAcc                                                                                >  1:1904488/1‑150 (MQ=255)
                                                                                ttGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGc                                                                      <  1:3023200/150‑1 (MQ=255)
                                                                                  gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCGAAACCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCCAATATTGTTACCCCAGCAACAAAAACGCTCATACAGCCCCAAACCCATTCATGGCGa                                                                    >  2:558499/1‑150 (MQ=38)
                                                                                     aGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATgg                                                                 <  2:5212467/150‑1 (MQ=255)
                                                                                       gTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCGTTTCCCCACACCCGAACCCTCTCCCCAGAGGGGCGCGGGGACCGTATTGTGCAAATAATGTTACCCCAGCAACAAACAGGCTCCTACAGCCCCCAACCCGTTCATGGCGATGGcg                                                               >  2:5138569/1‑149 (MQ=255)
                                                                                           gACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa                                                           <  2:534860/150‑1 (MQ=255)
                                                                                            aCCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCAAACCCGCGCCCCAGAGGGGCGCGGGGACCGTAGAGTGCAAATAATGTTACCCCCGCAACAAACACCCAACTACCCCCCCAAACCCATGCAAGGCCATGGCAGGGAc                                                          >  2:3082585/1‑150 (MQ=255)
                                                                                                   cAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAg                                                   <  1:3822986/150‑1 (MQ=255)
                                                                                                   cAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAg                                                   <  1:2384616/150‑1 (MQ=255)
                                                                                                      gCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGAcc                                                <  1:3571011/150‑1 (MQ=255)
                                                                                                       cTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGCGGGGCGTGGGGACCCTATTGTGCAAATATTGTTAACCCAGCAACAAACAGGCTAATACAGCCCCGAACCCATTCATTGCGATGGCTGGGACGGTTCAGACCt                                               >  2:3287634/1‑150 (MQ=38)
                                                                                                          cGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGc                                            <  2:1938185/150‑1 (MQ=255)
                                                                                                          cGGCAGGGGGGGCGCTTTTCCCCTCACCCGAACCCTCTCCCCCGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATCCAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGc                                            <  2:334598/150‑1 (MQ=255)
                                                                                                           ggCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGcc                                           <  1:2258555/150‑1 (MQ=255)
                                                                                                               gggggggCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAat                                       <  1:2201214/150‑1 (MQ=255)
                                                                                                                  ggggCGCTTTTCCCCTCACCCAAAACCACAACCCAGAGGGGCGAGGGGACCGTATTGTGCCAAAAATGTTACCCCAGCCACAAACAGGCTCAAACAGCCCCGAACCCATTCAAGGCGATGGCTGGGACGGTTCAGACCGTTCCGAATAtt                                    >  2:4211584/1‑150 (MQ=255)
                                                                                                                   gggCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAT‑‑TGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATtctc                                 <  2:797651/150‑1 (MQ=255)
                                                                                                                     gcgcTTTTCCCCGCCCCCTAAACCAATCCCCAGAGGGGCGAGGGGACCCGATTTTGCAAATATTTTTACCCCAGCAACAAAAACGCTCATAAATCCCCAAACCCTTTCATGGCGATGGCTGGGACGGGTCAGACCATTCCGAATATtctc                                 >  2:2542917/1‑150 (MQ=255)
                                                                                                                        cTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAg                              <  2:1636504/150‑1 (MQ=255)
                                                                                                                          tttCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCa                            <  2:1214967/150‑1 (MQ=255)
                                                                                                                               ccTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGtc                       <  1:2418927/150‑1 (MQ=255)
                                                                                                                               ccTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGtc                       >  2:3222402/1‑150 (MQ=255)
                                                                                                                                   aCCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCa                   >  1:5562006/1‑150 (MQ=255)
                                                                                                                                   aCCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCa                   <  1:5377374/150‑1 (MQ=255)
                                                                                                                                     ccTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGGCGAATATTCTCCAGCACCGTCTCCATg                 >  2:5048374/1‑150 (MQ=255)
                                                                                                                                     ccTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATg                 >  1:1024347/1‑150 (MQ=255)
                                                                                                                                      cTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGt                >  2:1384332/1‑150 (MQ=255)
                                                                                                                                       tAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAATAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGtt               >  2:1279797/1‑150 (MQ=255)
                                                                                                                                       gtccccTCGCCCCTCTGGGGAGAGGGTACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGtt               >  1:3352914/4‑150 (MQ=255)
                                                                                                                                        aaCCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGttt              >  1:770436/1‑150 (MQ=255)
                                                                                                                                           ccTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTca           <  2:2871086/150‑1 (MQ=255)
                                                                                                                                           ccTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTca           >  1:4062226/1‑150 (MQ=255)
                                                                                                                                            ctctCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcac          >  2:5012367/1‑150 (MQ=255)
                                                                                                                                            ctctCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcac          >  2:2220868/1‑150 (MQ=255)
                                                                                                                                             tctcCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcacc         >  1:3500293/1‑150 (MQ=255)
                                                                                                                                             tctcCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcacc         >  2:2749209/1‑150 (MQ=255)
                                                                                                                                               tcCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcaccac       <  2:3011764/150‑1 (MQ=255)
                                                                                                                                                  ccAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacgac    >  2:1032864/1‑150 (MQ=255)
                                                                                                                                                  ccAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaac    >  2:37276/1‑150 (MQ=255)
                                                                                                                                                  ccAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaac    >  1:3981143/1‑150 (MQ=255)
                                                                                                                                                   cAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCGTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaaca   <  2:23320/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
ATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAG  >  NC_000913/1351079‑1351376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: