Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,351,228 G→C 7.8% intergenic (‑189/+179) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,351,2280GC 7.8% 942.1 / 20.1 294intergenic (‑189/+179)fabI/ycjDenoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
Reads supporting (aligned to +/- strand):  ref base G (116/150);  new base C (23/4);  total (140/154)
Fisher's exact test for biased strand distribution p-value = 3.00e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 302 total aligned reads displayed.

ATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGC  >  NC_000913/1351079‑1351377
                                                                                                                                                     |                                                                                                                                                       
         ttCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAg                                                                                                                                               <  1:2899660/150‑1 (MQ=255)
          tcgtcgTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTGCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAgg                                                                                                                                              <  1:494312/150‑1 (MQ=255)
             tcgtTTTAGTAAACAGTACGAACAGCTAAACGGTTATTATACTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGa                                                                                                                                           <  2:2662455/150‑1 (MQ=255)
              cgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGAc                                                                                                                                          <  1:3216660/150‑1 (MQ=255)
                ttttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCg                                                                                                                                        >  2:5691374/1‑150 (MQ=255)
                 tttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTGCCCTCACCCTAACCCTCTCCCCAGTGGGGGGAGGGGTCCGt                                                                                                                                       >  2:4791098/1‑150 (MQ=255)
                  ttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa                                                                                                                                      <  1:3408603/150‑1 (MQ=255)
                    aGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAtt                                                                                                                                    <  1:5291293/150‑1 (MQ=255)
                    aGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCAACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAtt                                                                                                                                    <  2:2043481/150‑1 (MQ=255)
                      tAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgt                                                                                                                                  <  2:1319911/150‑1 (MQ=255)
                      tAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgt                                                                                                                                  >  2:2933217/1‑150 (MQ=255)
                       aaaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgtg                                                                                                                                 <  1:4087589/150‑1 (MQ=255)
                       aaaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgtg                                                                                                                                 <  2:5479725/150‑1 (MQ=255)
                         aCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCa                                                                                                                               <  1:1115778/150‑1 (MQ=255)
                         aCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCa                                                                                                                               <  2:1792462/150‑1 (MQ=255)
                         aCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCa                                                                                                                               <  1:1688544/150‑1 (MQ=255)
                         aCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAAACCAATCCCCAGAGGGGCGAGGGGCCCGTATTGTGCa                                                                                                                               >  2:1885020/1‑150 (MQ=255)
                          cAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCCGAGGGGCGAGGGGACCGTATTGGGCaa                                                                                                                              >  2:3806615/1‑150 (MQ=255)
                          cAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGcca                                                                                                                              >  1:1414161/1‑148 (MQ=255)
                               cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATAtt                                                                                                                        <  1:2550480/150‑1 (MQ=255)
                               cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATAtt                                                                                                                        <  1:5509204/150‑1 (MQ=255)
                               cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCGTTTCCCCGCACCCAAACCCTCTCCCCAGAGGGGCGAGGGGCCCGTATTGTGCAAAAAtt                                                                                                                        >  1:4321651/1‑150 (MQ=255)
                                gAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAACCCTCTCCCCAGAGGGGCGAGGGGACCGGATTGTGCAAAAATTg                                                                                                                       >  2:3230642/1‑150 (MQ=255)
                                gAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCAAACCCACTCCCCACAGGGGCGAGGGGACCCTATTGTGCAAataatt                                                                                                                        >  2:323837/1‑147 (MQ=255)
                                    aGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAc                                                                                                                   <  1:5913371/150‑1 (MQ=255)
                                        aaaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCa                                                                                                               <  1:1241950/150‑1 (MQ=255)
                                        aaaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAAACCACACCCCAGAGGGGCGAGGGGACCGTATTGTGCAAAAAATTTTAACCCa                                                                                                               >  1:1343703/1‑150 (MQ=255)
                                        aaaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAACCCACTACCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTTTTAccccc                                                                                                               >  1:832745/1‑149 (MQ=255)
                                         aaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCCCCACCAAAACCAAAACCCACAGGGGCGCGGGGGCCCGATTTTGGAAAAATATTTTAcccac                                                                                                               >  1:2092643/1‑148 (MQ=255)
                                          aCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCACACCCAAACCCACTACCCAGCGGGGCGAGGGGCCCGTATTGTGGCAAAATTGTTACCCCAGc                                                                                                             >  1:4735800/1‑150 (MQ=255)
                                            ggTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGcaa                                                                                                           >  2:5945193/1‑150 (MQ=255)
                                                attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                                                                                       <  1:2012545/150‑1 (MQ=255)
                                                attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                                                                                       <  2:15809/150‑1 (MQ=255)
                                                 ttatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaac                                                                                                      <  1:657134/150‑1 (MQ=255)
                                                 ttatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAGTGTGCAAATATTGTTACCCCAGCaacaaac                                                                                                      >  2:4475988/1‑150 (MQ=255)
                                                 ttatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAAACCTCACCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATAATTTTAACCCCGCaacaaac                                                                                                      >  2:3586672/1‑150 (MQ=39)
                                                   ataATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAg                                                                                                    <  1:3047582/150‑1 (MQ=255)
                                                    taATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAgg                                                                                                   <  1:2993979/150‑1 (MQ=255)
                                                    taATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAgg                                                                                                   <  1:3739933/150‑1 (MQ=255)
                                                     aaTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGc                                                                                                  >  2:3388701/1‑150 (MQ=255)
                                                       tCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCACCGCACCCGAACCCACGCCCCCGGGGGGGGCGGGGGCCGGATTGTGGCAAAATTGGTTCCCCAGCACCCACCACGCTc                                                                                                >  2:2284686/1‑150 (MQ=255)
                                                       tCAACCTGGCTGTGAGTAGATATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTc                                                                                                <  1:4825111/150‑1 (MQ=255)
                                                         aaCCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCAt                                                                                              <  2:1876177/150‑1 (MQ=255)
                                                         aaCCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCACACCCAAAACCAAACCCCAGAGGGGGGAGGGGGCCCGCATTTTCAAAAATTTTTTACCCCCCAACAAACAAGcacaa                                                                                              >  1:2431063/1‑149 (MQ=255)
                                                          aCCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATa                                                                                             <  2:2465219/150‑1 (MQ=255)
                                                            cTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACa                                                                                           <  1:5177713/150‑1 (MQ=255)
                                                            cTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAAACCAAACCCCAAAGGGGCGAGGGGACCCAAATTTGCAAAAAATTTTAACCCAACAACAAAAAAGCTCATACa                                                                                           >  1:1462525/1‑150 (MQ=255)
                                                             tGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAg                                                                                          >  2:3502453/1‑150 (MQ=255)
                                                             tGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAAACCACAACCCCCACGGGCGAGGGGGCCCTAATGTGCAAAAAATGTTAACCCACCCACAAACACGCCCaaaaac                                                                                          >  1:1303187/1‑145 (MQ=255)
                                                              ggCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGc                                                                                         <  1:935101/150‑1 (MQ=255)
                                                              ggCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGc                                                                                         <  2:4626752/150‑1 (MQ=255)
                                                              ggCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCAGCCGCAGGGGGGGCGCATTTCCCCGCACCCAAACCCGCTCCCCCGCCGGGCGAGCGGACCGTCTTGTGCACATATTGTTACGCCAGCGACACACACGCGCATACAGc                                                                                         >  2:846428/1‑150 (MQ=255)
                                                               gCTGTGAGTAGCTATAGGTGCCAGGTCCGACCGGAGCTGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCCCATACAGcc                                                                                        <  1:1573648/150‑1 (MQ=255)
                                                                cTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGccc                                                                                       <  1:2328479/150‑1 (MQ=255)
                                                                 tgtgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACACAAAACCACTCACCACAGGGGCCACGGGTCCCGAATGTTCAAAAAATTTTAAACCAGAAACAAAAACGCGCAAACAAcccc                                                                                      >  2:2820988/1‑150 (MQ=255)
                                                                   tgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTa                                                                                    <  1:4668421/150‑1 (MQ=255)
                                                                   tgAGTAGCTATAGGTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTa                                                                                    <  2:401518/150‑1 (MQ=255)
                                                                    gAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTaa                                                                                   <  1:2954275/150‑1 (MQ=255)
                                                                    gAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTaa                                                                                   <  1:4401694/150‑1 (MQ=255)
                                                                      gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAAACCACACCCCAGAGGGGCGAGGGGGCCCTATTGTGCCAAAAATGTTACCCCACCAACAAACAGGCTCAAACACCCCCAAAcc                                                                                 >  1:5761171/1‑150 (MQ=255)
                                                                      gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCAAACCCAAAACCAAAACCCCCAGGGGCGCGGGGGCCCGATTTTTCAAATAATTTTAACCCACCAACAAAAAAGCGCAAAAAGCCCCCAAcc                                                                                 >  1:1904488/1‑150 (MQ=255)
                                                                                  gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCGAAACCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCCAATATTGTTACCCCAGCAACAAAAACGCTCATACAGCCCCAAACCCATTCATGGCGa                                                                     >  2:558499/1‑150 (MQ=38)
                                                                                     aGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATgg                                                                  <  2:5212467/150‑1 (MQ=255)
                                                                                       gTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCGTTTCCCCACACCCGAACCCTCTCCCCAGAGGGGCGCGGGGACCGTATTGTGCAAATAATGTTACCCCAGCAACAAACAGGCTCCTACAGCCCCCAACCCGTTCATGGCGATGGcg                                                                >  2:5138569/1‑149 (MQ=255)
                                                                                           gACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa                                                            <  2:534860/150‑1 (MQ=255)
                                                                                            aCCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCAAACCCGCGCCCCAGAGGGGCGCGGGGACCGTAGAGTGCAAATAATGTTACCCCCGCAACAAACACCCAACTACCCCCCCAAACCCATGCAAGGCCATGGCAGGGAc                                                           >  2:3082585/1‑150 (MQ=255)
                                                                                                   cAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAg                                                    <  1:3822986/150‑1 (MQ=255)
                                                                                                    aGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGa                                                   <  1:2695112/150‑1 (MQ=255)
                                                                                                      gCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGAcc                                                 <  1:3571011/150‑1 (MQ=255)
                                                                                                       cTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGCGGGGCGTGGGGACCCTATTGTGCAAATATTGTTAACCCAGCAACAAACAGGCTAATACAGCCCCGAACCCATTCATTGCGATGGCTGGGACGGTTCAGACCt                                                >  2:3287634/1‑150 (MQ=38)
                                                                                                          cGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGc                                             <  2:1938185/150‑1 (MQ=255)
                                                                                                          cGGCAGGGGGGGCGCTTTTCCCCTCACCCGAACCCTCTCCCCCGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATCCAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGc                                             <  2:334598/150‑1 (MQ=255)
                                                                                                           ggCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGcc                                            <  1:2258555/150‑1 (MQ=255)
                                                                                                               gggggggCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAat                                        <  1:2201214/150‑1 (MQ=255)
                                                                                                                  ggggCGCTTTTCCCCTCACCCAAAACCACAACCCAGAGGGGCGAGGGGACCGTATTGTGCCAAAAATGTTACCCCAGCCACAAACAGGCTCAAACAGCCCCGAACCCATTCAAGGCGATGGCTGGGACGGTTCAGACCGTTCCGAATAtt                                     >  2:4211584/1‑150 (MQ=255)
                                                                                                                   gggCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAT‑‑TGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATtctc                                  <  2:797651/150‑1 (MQ=255)
                                                                                                                     gcgcTTTTCCCCGCCCCCTAAACCAATCCCCAGAGGGGCGAGGGGACCCGATTTTGCAAATATTTTTACCCCAGCAACAAAAACGCTCATAAATCCCCAAACCCTTTCATGGCGATGGCTGGGACGGGTCAGACCATTCCGAATATtctc                                  >  2:2542917/1‑150 (MQ=255)
                                                                                                                        cTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAg                               <  2:1636504/150‑1 (MQ=255)
                                                                                                                          tttCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCa                             <  2:1214967/150‑1 (MQ=255)
                                                                                                                               ccTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGtc                        >  2:3222402/1‑150 (MQ=255)
                                                                                                                               ccTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGtc                        <  1:2418860/150‑1 (MQ=255)
                                                                                                                                   aCCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCa                    >  1:5562006/1‑150 (MQ=255)
                                                                                                                                   aCCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCa                    <  1:5377374/150‑1 (MQ=255)
                                                                                                                                     ccTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGGCGAATATTCTCCAGCACCGTCTCCATg                  >  2:5048374/1‑150 (MQ=255)
                                                                                                                                     ccTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATg                  >  1:1024347/1‑150 (MQ=255)
                                                                                                                                      cTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGt                 >  2:1384332/1‑150 (MQ=255)
                                                                                                                                       tAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAATAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGtt                >  2:1279797/1‑150 (MQ=255)
                                                                                                                                       gtccccTCGCCCCTCTGGGGAGAGGGTACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGtt                >  1:3352914/4‑150 (MQ=255)
                                                                                                                                        aaCCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGttt               >  1:770436/1‑150 (MQ=255)
                                                                                                                                           ccTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTca            >  1:4062226/1‑150 (MQ=255)
                                                                                                                                           ccTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTca            <  2:2871086/150‑1 (MQ=255)
                                                                                                                                            ctctCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcac           >  2:5012367/1‑150 (MQ=255)
                                                                                                                                            ctctCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcac           >  2:2220868/1‑150 (MQ=255)
                                                                                                                                             tctcCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcacc          >  2:2749209/1‑150 (MQ=255)
                                                                                                                                             tctcCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcacc          >  1:3500293/1‑150 (MQ=255)
                                                                                                                                               tcCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcaccac        <  2:3011764/150‑1 (MQ=255)
                                                                                                                                                  ccAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacgac     >  2:1032864/1‑150 (MQ=255)
                                                                                                                                                  ccAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaac     >  1:3981143/1‑150 (MQ=255)
                                                                                                                                                  ccAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaac     >  2:37276/1‑150 (MQ=255)
                                                                                                                                                   cAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCGTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaaca    <  2:23320/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
ATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGC  >  NC_000913/1351079‑1351377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: