Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,864,763 A→T 5.8% intergenic (+29/+19) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,864,7630AT 5.8% 895.6 / 30.8 280intergenic (+29/+19)yeaD/yeaED‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (153/109);  new base T (6/12);  total (159/121)
Fisher's exact test for biased strand distribution p-value = 4.87e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

Only 100 of 292 total aligned reads displayed.

GCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTT  >  NC_000913/1864614‑1864912
                                                                                                                                                     |                                                                                                                                                     
gCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCaa                                                                                                                                                       <  2:2078997/150‑1 (MQ=255)
 ccGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAAt                                                                                                                                                      >  2:3416039/1‑150 (MQ=255)
 ccGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAAt                                                                                                                                                      <  1:5282251/150‑1 (MQ=255)
 ccGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAAt                                                                                                                                                      >  1:2187558/1‑150 (MQ=255)
         tgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCgg                                                                                                                                              <  1:1618993/150‑1 (MQ=255)
         tgGCTACAAAACATTTGTTCGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCgg                                                                                                                                              <  2:2054814/150‑1 (MQ=255)
          ggCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGc                                                                                                                                             >  1:935935/1‑150 (MQ=255)
           gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGca                                                                                                                                            >  1:3450066/1‑150 (MQ=255)
           gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGca                                                                                                                                            <  2:1108645/150‑1 (MQ=255)
            cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcac                                                                                                                                           >  1:5017681/1‑150 (MQ=255)
                aaaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACacaa                                                                                                                                       >  1:2657003/1‑150 (MQ=255)
                       ttgtttgtGTAGAACCGGCTTAGGCTTCAGAAACGCTATACGTGACCAAAGAGAAACCTGCACTTGTGGCGCAATCCTTTCGGGTTGCGAAAGGTTATTTTACGTTAATGTTGTGTGCGGGGTGAAATGCAGCGGGCACACAACATcaca                                                                                                                                <  2:2102414/150‑1 (MQ=255)
                       ttgtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATcaca                                                                                                                                >  2:201188/1‑150 (MQ=255)
                       ttgtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATcaca                                                                                                                                <  2:5498266/150‑1 (MQ=255)
                          tttgtGTAGAAACGGCTTACGCTTCAGAAACTCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTATTTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGCACACAACAttaacgta                                                                                                                             <  2:62199/150‑8 (MQ=38)
                          tttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCTTTGCGAAACGTTAATTTACGTTATTGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCa                                                                                                                             <  2:4318996/150‑1 (MQ=255)
                                tAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCaa                                                                                                                                                       <  2:405549/118‑1 (MQ=255)
                                tAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCa                                                                                                                       >  1:173279/1‑150 (MQ=255)
                                tAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATAAccc                                                                                                                       >  2:2712136/1‑149 (MQ=255)
                                  gAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgc                                                                                                                     >  2:1826659/1‑150 (MQ=255)
                                   aaaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgcg                                                                                                                    >  1:4402236/1‑150 (MQ=255)
                                     aCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCa                                                                                                                  >  1:1007579/1‑150 (MQ=255)
                                       ggCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGt                                                                                                                <  2:5092559/150‑1 (MQ=255)
                                        gCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGtt                                                                                                               >  1:1878981/1‑150 (MQ=255)
                                         cTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCTTTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGttt                                                                                                              <  2:2553583/150‑1 (MQ=255)
                                          ttACGCTTCAGAAACGCAACAAGTGACCACAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAACCGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACCCCATATCCAGCGCAGtttt                                                                                                             <  2:5319432/150‑1 (MQ=255)
                                          ttACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGtttt                                                                                                             >  2:307367/1‑150 (MQ=255)
                                          ttACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGtttt                                                                                                             >  2:3172147/1‑150 (MQ=255)
                                          ttACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGtttt                                                                                                             >  1:4167664/1‑150 (MQ=255)
                                            aCGCTTCAGAAACGCAAAAAGTGACAAAAGAGAAACCTGCAAATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTc                                                                                                           <  2:5537911/150‑1 (MQ=255)
                                              gCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTATTGTTGTGTGGCGGGTGGAATGCATACGGCACACAAAATCACACCATATACAGCGCAGttttttgt                                                                                                         >  1:2132146/1‑147 (MQ=255)
                                              gCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACCTCACACCATATCCAGCGCAGTTTTTccg                                                                                                         >  1:4281492/1‑149 (MQ=255)
                                                ttCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTGCttt                                                                                                       >  1:2710069/1‑150 (MQ=255)
                                                ttCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtgt                                                                                                       >  2:4234691/1‑148 (MQ=255)
                                                ttCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtgt                                                                                                       >  1:3989662/1‑148 (MQ=255)
                                                 tCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtttt                                                                                                      <  2:173582/150‑1 (MQ=255)
                                                    gAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACCCAACATCACACCATATCCAGCGCCGTTTTTCCTTTTGGt                                                                                                   >  2:4874646/1‑150 (MQ=255)
                                                    gAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGt                                                                                                   >  1:3216503/1‑150 (MQ=255)
                                                     aaaCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCGTTTGGTg                                                                                                  >  2:5209973/1‑150 (MQ=255)
                                                        cGCAAAACGTGACCAACGAGAACCCTGCACATCTGGCGCAAGCCATTCGCGTGGCGAAACGTTAATTTCCGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCg                                                                                               <  2:1528573/150‑1 (MQ=255)
                                                        cGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCg                                                                                               <  2:5133867/150‑1 (MQ=255)
                                                           aaaaaGTGACCAAAGTGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTTCGTTATTGTTGTGTGGCGGGTGGTATGCATGCGGGAGTCAACATCACACCATATCGAGGGGAGTTTTTGGTTTTGGTgggggtt                                                                                            >  2:2816665/1‑144 (MQ=255)
                                                           aaaaaGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGat                                                                                            >  2:5421473/1‑150 (MQ=255)
                                                           aaaaaGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGCACACAACATCAAACCATATACAGCGCAGTTTTTCCTTTTGGTGCCGGat                                                                                            >  2:1847020/1‑150 (MQ=255)
                                                               aGTGACCAACGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGc                                                                                        <  2:5030575/150‑1 (MQ=255)
                                                                    ccAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCGTTTGGTGCCGGATATGCCTTAt                                                                                   >  1:4834602/1‑150 (MQ=255)
                                                                     cAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATc                                                                                  >  2:4633686/1‑150 (MQ=255)
                                                                     cAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATc                                                                                  >  2:3900323/1‑150 (MQ=255)
                                                                     cAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAAAGACTCAGGCACACAACAACAAACAATATACAGCGCAGTTTTTAAATTTGGTGCCGGATATGCCATATc                                                                                  >  1:1259712/1‑150 (MQ=255)
                                                                      aaaGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATcc                                                                                 >  1:3658398/1‑150 (MQ=255)
                                                                       aaGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCa                                                                                <  2:193150/150‑1 (MQ=255)
                                                                       aaGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCGGTTGGTGCCGGATATGCCTTATCCa                                                                                >  2:5808922/1‑150 (MQ=255)
                                                                       aaGAGAAACCTGCACATCTGGCGCAATCCATGCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCa                                                                                <  2:1584933/150‑1 (MQ=255)
                                                                          agaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGTATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCa                                                                             >  1:2845416/1‑150 (MQ=255)
                                                                          agaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGTATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCATTATCCAGCa                                                                             >  1:207712/1‑150 (MQ=255)
                                                                          agaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCGTTTGGTGCCGGATATGCCTTATCCAGCa                                                                             >  2:5923516/1‑150 (MQ=255)
                                                                            aaaCCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGGGCAATGCCACCGGCCCCCAACATCACACCAAATCCCGCCCAGTTTTTCCGTTTGGTGCCGGGTATGCCTTATCCAGCATa                                                                           >  1:2787290/1‑150 (MQ=255)
                                                                                 tGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTaa                                                                      >  2:564280/1‑150 (MQ=255)
                                                                                   cacaTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAAtt                                                                    >  2:3503474/1‑150 (MQ=255)
                                                                                      aTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCg                                                                 <  2:3658398/150‑1 (MQ=255)
                                                                                        cTGGCGCACTCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCGGGGTGCATTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCt                                                               <  2:1672846/150‑1 (MQ=255)
                                                                                        cTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCt                                                               <  2:1885230/150‑1 (MQ=255)
                                                                                         tGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCCGGTTTTCCGTTTGGTGCCGGATCTGCCTTATCCAGCATAGCTAATTCCGCTg                                                              >  2:4188402/1‑150 (MQ=255)
                                                                                           gcgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaa                                                            <  2:2700620/150‑1 (MQ=255)
                                                                                           gcgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTACGCTgaa                                                            >  2:1336920/1‑150 (MQ=255)
                                                                                           gcccAATCCATTCGCGTTGCTAAATTTTAATTACTTTTATTTTTTTGTGCGGGGTGCATGCCAGCGGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaa                                                            <  1:515980/147‑1 (MQ=255)
                                                                                              cAATCCATTCGCTTTGCTAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaagaa                                                         <  1:3800639/150‑1 (MQ=255)
                                                                                               aaTCCATTCGCGTTGCGAAACTTTAATTTACGTTATTTTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGaaa                                                        <  1:629757/150‑1 (MQ=255)
                                                                                                aTCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGACAg                                                       >  2:4981050/1‑150 (MQ=255)
                                                                                                aTCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAg                                                       <  2:2691366/150‑1 (MQ=255)
                                                                                                   cATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCAGCTCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTc                                                    <  2:945987/150‑1 (MQ=255)
                                                                                                     ttCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGa                                                  <  2:173279/150‑1 (MQ=255)
                                                                                                     ttCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGa                                                  <  2:1624021/150‑1 (MQ=255)
                                                                                                     ttCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCGTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGa                                                  >  2:2202622/1‑150 (MQ=255)
                                                                                                          gTTGCGAAACTTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGAATAGCTAATTCCGCTGAAGAAAGTTCGACCTCa                                             <  2:4559878/150‑1 (MQ=255)
                                                                                                           ttGCGAAACGTTAATTTACGTTATTGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCaa                                            <  1:3682784/150‑1 (MQ=255)
                                                                                                            tGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAg                                           <  1:4500728/150‑1 (MQ=255)
                                                                                                             gCGAAACGTTAATTTCCGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGc                                          <  2:1475131/150‑1 (MQ=255)
                                                                                                               gAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGcac                                        >  2:738152/1‑150 (MQ=255)
                                                                                                                aaaCGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGcaca                                       <  2:5001954/150‑1 (MQ=255)
                                                                                                                 aaCGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCGCACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAg                                      <  1:2933184/150‑1 (MQ=255)
                                                                                                                 aaCGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCGCACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAg                                      <  1:5897810/150‑1 (MQ=255)
                                                                                                                        aTTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAtt                               <  1:30459/150‑1 (MQ=255)
                                                                                                                             cGTTATTGTTGTGTGCCGGGTGCATTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                          <  1:3592607/150‑1 (MQ=255)
                                                                                                                             cGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                          <  1:885342/150‑1 (MQ=255)
                                                                                                                             cGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                          <  1:4369253/150‑1 (MQ=255)
                                                                                                                                ttaTGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATCTCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCTAGCACAGCCGCATTTTGTTGGa                       <  2:3270363/148‑1 (MQ=255)
                                                                                                                                     ttgtGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATgg                  <  2:2176578/150‑1 (MQ=255)
                                                                                                                                     ttgtGTGCCGGGAGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATgg                  <  2:3013891/150‑1 (MQ=255)
                                                                                                                                      ggtgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATggg                 <  1:5617845/149‑1 (MQ=255)
                                                                                                                                          tgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAAt             >  1:2725523/1‑150 (MQ=255)
                                                                                                                                          tgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAAt             >  1:4883757/1‑150 (MQ=255)
                                                                                                                                           gCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc            >  2:1072576/1‑150 (MQ=255)
                                                                                                                                            ccGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCg           >  2:5971206/1‑150 (MQ=255)
                                                                                                                                            ccGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCg           >  2:4384482/1‑150 (MQ=255)
                                                                                                                                              gggTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTg         <  1:3977473/150‑1 (MQ=255)
                                                                                                                                              gggTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTg         <  2:3859371/150‑1 (MQ=255)
                                                                                                                                               ggTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTgg        >  1:1772689/1‑150 (MQ=255)
                                                                                                                                                   cAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCACTCGTGGCCGc    >  2:5670127/1‑150 (MQ=255)
                                                                                                                                                    aaTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCt   >  1:5693809/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTT  >  NC_000913/1864614‑1864912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: