Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,864,768 T→C 6.7% intergenic (+34/+14) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,864,7680TC 6.7% 845.5 / 31.0 280intergenic (+34/+14)yeaD/yeaED‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base T (150/102);  new base C (7/11);  total (162/118)
Fisher's exact test for biased strand distribution p-value = 1.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.53e-01

Only 100 of 286 total aligned reads displayed.

ATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGA  >  NC_000913/1864619‑1864917
                                                                                                                                                     |                                                                                                                                                     
    tgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCgg                                                                                                                                                   <  1:1618986/150‑1 (MQ=255)
    tgGCTACAAAACATTTGTTCGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCgg                                                                                                                                                   <  2:2054814/150‑1 (MQ=255)
      gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGca                                                                                                                                                 <  2:3320595/150‑1 (MQ=255)
       cTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcac                                                                                                                                                >  1:4868609/1‑150 (MQ=255)
                ttttgtttgTGTAGAAACGGCTTACCCTTCATAAACCCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATCCGCGTTGCGAAACGTTAATTTACGTTATTGTTGTGTGCCGGATGAATTGCACCCGCCACACAACAtta                                                                                                                                       <  2:5522286/149‑3 (MQ=255)
                  ttgtttgtGTAGAACCGGCTTAGGCTTCAGAAACGCTATACGTGACCAAAGAGAAACCTGCACTTGTGGCGCAATCCTTTCGGGTTGCGAAAGGTTATTTTACGTTAATGTTGTGTGCGGGGTGAAATGCAGCGGGCACACAACATcaca                                                                                                                                     <  2:2102414/150‑1 (MQ=255)
                  ttgtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATcaca                                                                                                                                     >  2:2294181/1‑150 (MQ=255)
                  ttgtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATcaca                                                                                                                                     >  2:201188/1‑150 (MQ=255)
                     tttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCTTTGCGAAACGTTAATTTACGTTATTGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCa                                                                                                                                  <  2:4318996/150‑1 (MQ=255)
                     tttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAACCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCa                                                                                                                                  <  2:1692941/150‑1 (MQ=255)
                           tAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATAAccc                                                                                                                            >  2:2712136/1‑149 (MQ=255)
                             gAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgc                                                                                                                          >  2:4679560/1‑150 (MQ=255)
                             gAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgc                                                                                                                          >  2:1826659/1‑150 (MQ=255)
                              aaaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgcg                                                                                                                         >  1:4402236/1‑150 (MQ=255)
                              aaaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgcg                                                                                                                         >  2:3876092/1‑150 (MQ=255)
                              aaaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgcg                                                                                                                         >  1:5940932/1‑150 (MQ=255)
                                aCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCa                                                                                                                       >  1:1007579/1‑150 (MQ=255)
                                   gCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGtt                                                                                                                    >  2:1218620/1‑150 (MQ=255)
                                      tACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCCCACAACATCACACCATATACAGCGCAGttttt                                                                                                                 >  1:3583440/1‑150 (MQ=255)
                                         gCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTATTGTTGTGTGGCGGGTGGAATGCATACGGCACACAAAATCACACCATATACAGCGCAGttttttgt                                                                                                              >  1:2132146/1‑147 (MQ=255)
                                         gCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCCGCGCAGTTTTTCCt                                                                                                              >  1:3682431/1‑150 (MQ=255)
                                           ttCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtgt                                                                                                            >  2:4234691/1‑148 (MQ=255)
                                           ttCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtgt                                                                                                            >  1:3989662/1‑148 (MQ=255)
                                           ttCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATAACCCGCGCAGtttttgcgtt                                                                                                            >  1:5578570/1‑145 (MQ=255)
                                            tCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtttt                                                                                                           <  2:173492/150‑1 (MQ=255)
                                               gAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGt                                                                                                        >  1:3216503/1‑150 (MQ=255)
                                                aaaCGCAAAAAGTGACCAAAGAGAAACCTGCCCATCTGGCGCAATCCATGCGCGTTGCGAATCGTTAATTTACGTTAATGTTGTGTGCCGGGGGCAATGCATCCGGCACACCACATCACACCATATCCAGCGCAGTGTTTCCTTTTGGTg                                                                                                       >  2:599997/1‑150 (MQ=255)
                                                aaaCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTg                                                                                                       >  1:3328430/1‑150 (MQ=255)
                                                aaaCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTg                                                                                                       >  2:941213/1‑150 (MQ=255)
                                                aaaCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCGTTTGGTg                                                                                                       >  2:5209973/1‑150 (MQ=255)
                                                   cGCAAAAAGTGACCAAAGAGAAACCTGCCCATCTGGCGCAATCCAGTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCg                                                                                                    <  2:5589371/150‑1 (MQ=255)
                                                   cGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCg                                                                                                    <  2:5133867/150‑1 (MQ=255)
                                                    gCAACATGTCACCAACGAGAACCCTGCCCATGGGGCTAAATCCATTCGTGTTGCGAAAGGTTAATTAACTTTAATGTTGTGTGCCGGGTGCAATGCCTCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTTCCgg                                                                                                   <  2:5033278/150‑1 (MQ=255)
                                                    gCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCCCACAACATCACACCATATCCAGCGCAGTTTTTGCTTTTGGTGCCgg                                                                                                   >  1:1761713/1‑150 (MQ=255)
                                                     cAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGCCCACAACAGCACACCATATACAGCGCAGTTTTTCGTTTTGGTGCCGGa                                                                                                  >  1:5836422/1‑150 (MQ=255)
                                                      aaaaaGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATACGGCACACAACATCAAACCATATACAGCGCAGTTTTTCCTTTTGGTGCCGGat                                                                                                 >  2:1847020/1‑150 (MQ=255)
                                                              aCCAAAGAGAAACCTGCACATCTGGCGCAATCCATGCGCGTTGCTAAACTTAAATTTCCGTTATTGTTGTGTGCCGGGTGAAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTa                                                                                         <  1:3779512/150‑1 (MQ=255)
                                                               ccAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCGTTTGGTGCCGGATATGCCTTAt                                                                                        >  1:4834602/1‑150 (MQ=255)
                                                                cAAAGAGAAACCTCCACATCTGGCGCAATCCAATCGCGTTGCGAAACGTTAATTTAAGTTAATGTTGTGTGCCGGGTGCAATGCATCCCGCACCCAACATCACACCAGATCCAGCGCAGTTTGGCCGTGGGGTGCCGGCTGTGCCATATc                                                                                       >  2:5098557/1‑150 (MQ=255)
                                                                 aaaGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACAACACACCATATCCCGCGCAGTTTTTCCTTTTGGTGCCGGATATTCCTTATcc                                                                                      >  1:2712357/1‑150 (MQ=255)
                                                                  aaGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCa                                                                                     >  2:695956/1‑150 (MQ=255)
                                                                  aaGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCa                                                                                     <  2:193150/150‑1 (MQ=255)
                                                                  aaGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCGTTTGGTGCCGGATATGCCTTATCCa                                                                                     >  2:1553352/1‑150 (MQ=255)
                                                                     agaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGTATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCa                                                                                  >  1:2845416/1‑150 (MQ=255)
                                                                     agaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGTATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCATTATCCAGCa                                                                                  >  1:207712/1‑150 (MQ=255)
                                                                       aacccTGCACATCGGGCGCAATCCATTCGCGTTGCTAAACTTTATTTTAGTTTATTGTTGTGTGCCGGGTGCAAGGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATa                                                                                <  1:928601/147‑1 (MQ=255)
                                                                       aaaCCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGGCGGGTGCAATGCATCCGGCCCACAACATCAAACCAAATCCAGCGCAGTTTTTGCTTTTGGTGCCGGGTATTCCTTATCCAGCATa                                                                                >  1:2787138/1‑150 (MQ=255)
                                                                           cTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATTCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTa                                                                            >  2:1602452/1‑150 (MQ=255)
                                                                              cacaTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAAtt                                                                         >  2:3503474/1‑150 (MQ=255)
                                                                              cacaTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCGTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAAtt                                                                         >  2:1665538/1‑150 (MQ=255)
                                                                                caTCGGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGGGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTcc                                                                       <  1:567988/150‑1 (MQ=255)
                                                                                 aTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCg                                                                      <  2:3658398/150‑1 (MQ=255)
                                                                                   cTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCt                                                                    <  2:1885230/150‑1 (MQ=255)
                                                                                   cTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCt                                                                    <  2:5853974/150‑1 (MQ=255)
                                                                                     ggCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGGCGGGTGCAATGCCACCGGCACACAACCTCACCCCATAACCAGCGCCGTTTTTCCGTTTTGTGCCGGCTATGGCTTATCCAGGCTAGCTAAATACGCGga                                                                  >  1:5102794/1‑150 (MQ=255)
                                                                                      gcgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCCGCGCAGTTTTTCCTGTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaa                                                                 >  2:1337271/1‑150 (MQ=255)
                                                                                      gcgcAATCCATGCGCGTTGCGAAACGTTAATTCACGTTAATGTGGGGGGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaa                                                                 <  1:5181699/150‑1 (MQ=255)
                                                                                         cAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAAAGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCCGATATGCCGTATCCAGCATAGCAAAATCCGCTgaagaa                                                              >  2:149527/1‑150 (MQ=255)
                                                                                           aTCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATTCAAACGGCCCACAACAACAAACCAAATCCAGCGCCGTTTTTGCGTTTGGTGCCCGATATTGCGTATACAGCATATCAAATTACGCGGGAGAAAg                                                            >  1:773326/1‑150 (MQ=255)
                                                                                           aTCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAg                                                            <  2:2691288/150‑1 (MQ=255)
                                                                                              cATTCGCGTTGCGAAACGTTAATTTACGTGAATGTTGTGGGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTc                                                         <  2:946207/150‑1 (MQ=255)
                                                                                               aTTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGGCGGGTGCCATGCATCCGGCACACAAAATCACACCATATCCCGCGCAGTTTTTACTTTTGGTGGCGGGTATTCCTTATCCAGCCTAGCTAATTACCCTGAAGAAAGTTCg                                                        >  1:3624248/1‑150 (MQ=255)
                                                                                               aTTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCCTATCCAGCGCAGTTTTTCCGTTTGGTGCCGGATATGCCTTCTCCAGCATAGCTAATTCCGCTGAAGAAAGTGCg                                                        >  2:5003098/1‑150 (MQ=255)
                                                                                                ttCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCCGTTTTTCCGTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGa                                                       >  2:2202326/1‑150 (MQ=255)
                                                                                                    cgTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTc                                                   <  2:5940932/150‑1 (MQ=255)
                                                                                                     gTTGCGAAACTTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGAATAGCTAATTCCGCTGAAGAAAGTTCGACCTCa                                                  <  2:4559878/150‑1 (MQ=255)
                                                                                                       tGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAg                                                <  1:4500728/150‑1 (MQ=255)
                                                                                                        gCGAAACGTTAATTTCCGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGc                                               <  2:1475131/150‑1 (MQ=255)
                                                                                                         cGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGca                                              >  2:4983965/1‑150 (MQ=255)
                                                                                                           aaaCGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGcaca                                            <  2:2694178/150‑1 (MQ=255)
                                                                                                           aaaCGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGcaca                                            <  2:5319517/150‑1 (MQ=255)
                                                                                                           aaaCGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGcaca                                            <  2:323096/150‑1 (MQ=255)
                                                                                                            aaCGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCGCACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAg                                           <  1:2933184/150‑1 (MQ=255)
                                                                                                            aaCGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAg                                           <  1:4972933/150‑1 (MQ=255)
                                                                                                            aaCGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAg                                           <  1:167890/150‑1 (MQ=255)
                                                                                                              cGTTAATTTCCGTTAATGTTGTGTGCCGGGTGCAAGGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGcc                                         <  1:4185525/150‑1 (MQ=255)
                                                                                                               gTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCg                                        >  2:566612/1‑150 (MQ=255)
                                                                                                                 tAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGAACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCa                                      <  1:215467/150‑1 (MQ=255)
                                                                                                                   aTTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAtt                                    <  1:30459/150‑1 (MQ=255)
                                                                                                                        cGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                               <  1:4369253/150‑1 (MQ=255)
                                                                                                                        cGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                               <  2:1026246/150‑1 (MQ=255)
                                                                                                                                ttgtGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATgg                       <  2:2176578/150‑1 (MQ=255)
                                                                                                                                ttgtGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATgg                       >  2:5465008/1‑150 (MQ=255)
                                                                                                                                ttgtGTGCCGGGAGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATgg                       <  2:3013891/150‑1 (MQ=255)
                                                                                                                                 tgtgtgCCGGGTGCAATGCATCCGGAACACAACATCACACCATATCCAGCTCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATggg                      <  1:1847020/150‑1 (MQ=255)
                                                                                                                                       ccGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCg                >  1:2677336/1‑150 (MQ=255)
                                                                                                                                       ccGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCg                >  2:1093578/1‑150 (MQ=255)
                                                                                                                                       ccGGATGCATTGCACCCGGCACACAACATTACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCg                >  2:4167659/1‑150 (MQ=255)
                                                                                                                                       ccGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCg                >  2:4384482/1‑150 (MQ=255)
                                                                                                                                        cGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGt               >  2:3138042/1‑150 (MQ=255)
                                                                                                                                         gggTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTg              <  2:3859347/150‑1 (MQ=255)
                                                                                                                                         gggTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTg              <  1:3977473/150‑1 (MQ=255)
                                                                                                                                         gggTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTg              >  2:4025986/1‑150 (MQ=255)
                                                                                                                                              cAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCACTCGTGGCCGc         >  2:5670127/1‑150 (MQ=255)
                                                                                                                                              cAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAAATCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGc         >  2:4579749/1‑150 (MQ=255)
                                                                                                                                                aTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCtt       >  2:3071585/1‑150 (MQ=255)
                                                                                                                                                    aTCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTgg   >  2:4889046/1‑150 (MQ=255)
                                                                                                                                                    aTCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCGGTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAACGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACAGGGGCAAGCGTGGCCGcgggggg   >  2:2879816/1‑144 (MQ=255)
                                                                                                                                                     tCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGa  >  1:39045/1‑150 (MQ=255)
                                                                                                                                                     tCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGa  >  1:1231975/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGGA  >  NC_000913/1864619‑1864917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: