Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,028,159 T→A 5.4% intergenic (‑142/+29) yedQ ← / ← yodC putative membrane‑anchored diguanylate cyclase/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,028,1590TA 5.4% 1145.8 / 37.0 315intergenic (‑142/+29)yedQ/yodCputative membrane‑anchored diguanylate cyclase/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (142/156);  new base A (13/4);  total (155/160)
Fisher's exact test for biased strand distribution p-value = 2.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

Only 100 of 327 total aligned reads displayed.

TGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTGGAGTATCCA  >  NC_000913/2028010‑2028308
                                                                                                                                                     |                                                                                                                                                     
 gctgcACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGAtgtg                                                                                                                                                      >  2:2115129/1‑150 (MQ=255)
    gcACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATc                                                                                                                                                   >  2:253153/1‑150 (MQ=255)
      aCCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCg                                                                                                                                                 >  2:2360089/1‑150 (MQ=255)
       cccTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCgg                                                                                                                                                <  1:5787414/150‑1 (MQ=255)
       cccTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCgg                                                                                                                                                <  2:135323/150‑1 (MQ=255)
        ccTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGc                                                                                                                                               >  2:3452492/1‑150 (MQ=255)
        ccTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGc                                                                                                                                               <  2:2648514/150‑1 (MQ=255)
        ccTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGc                                                                                                                                               <  2:5172117/150‑1 (MQ=255)
          tGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCt                                                                                                                                             >  1:2802755/1‑150 (MQ=255)
          tGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCt                                                                                                                                             <  2:1790503/150‑1 (MQ=255)
            acgacTCCCACCCTTCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGa                                                                                                                                           <  2:3603225/150‑1 (MQ=255)
                cTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGtt                                                                                                                                       <  1:1293709/150‑1 (MQ=255)
                 tCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTa                                                                                                                                      >  1:3731859/1‑150 (MQ=255)
                 tCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTa                                                                                                                                      >  1:1500700/1‑150 (MQ=255)
                 tCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTa                                                                                                                                      >  1:5820124/1‑150 (MQ=255)
                  cccAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACAGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTaa                                                                                                                                     >  1:3554267/1‑150 (MQ=255)
                   ccAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAATCAGCGATAATTTTATAATGGTGGCATGATAAGACGCGTCTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAAt                                                                                                                                    <  1:4605225/150‑1 (MQ=255)
                    cAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATg                                                                                                                                   >  1:500476/1‑150 (MQ=255)
                    cAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATg                                                                                                                                   >  1:563777/1‑150 (MQ=255)
                    cAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATg                                                                                                                                   >  2:5773764/1‑150 (MQ=255)
                    cAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATg                                                                                                                                   >  1:1922060/1‑150 (MQ=255)
                     aaCCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGa                                                                                                                                  >  1:2189726/1‑150 (MQ=255)
                        cTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATAc                                                                                                                               <  1:970076/150‑1 (MQ=255)
                         tGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATAcc                                                                                                                              <  2:3541099/150‑1 (MQ=255)
                         tGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATAcc                                                                                                                              >  2:64640/1‑150 (MQ=255)
                         tGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATAcc                                                                                                                              <  1:2551568/150‑1 (MQ=255)
                          gCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATAccc                                                                                                                             >  1:4224012/1‑150 (MQ=255)
                          gCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCTGgtgtgc                                                                                                                                      >  2:49443/1‑139 (MQ=255)
                            ttttttATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCtt                                                                                                                           >  2:2712497/1‑150 (MQ=255)
                             tttttATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCttt                                                                                                                          <  2:4766560/150‑1 (MQ=255)
                                 tATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTcaca                                                                                                                      <  1:489105/150‑1 (MQ=255)
                                  aTGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCacac                                                                                                                     >  1:2116987/1‑150 (MQ=255)
                                       tCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACActtctt                                                                                                                >  1:2594126/1‑150 (MQ=255)
                                          ggCATAACCTGGCGATAGCGTACCGCAAAACATCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCg                                                                                                             <  1:2232376/150‑1 (MQ=255)
                                            cATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCa                                                                                                           <  1:5248155/150‑1 (MQ=255)
                                               aaCCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGaa                                                                                                        <  2:2410540/150‑1 (MQ=255)
                                                  cTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGa                                                                                                     <  2:451732/150‑1 (MQ=255)
                                                   tGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGAc                                                                                                    >  2:1533808/1‑150 (MQ=255)
                                                     gCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActc                                                                                                  >  2:5372147/1‑150 (MQ=255)
                                                             gTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACtcccctcccc                                                                                          >  2:4225951/1‑150 (MQ=255)
                                                              tACCGCAAAACAGCGATAATTTTATACTGGTGGCGTGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTccccc                                                                                         <  2:2825441/150‑1 (MQ=255)
                                                              tACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTccccc                                                                                         >  2:5883164/1‑150 (MQ=255)
                                                              tACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTccccc                                                                                         >  2:3829741/1‑150 (MQ=255)
                                                                  gCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGaa                                                                                     >  2:1507854/1‑150 (MQ=255)
                                                                   cAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATg                                                                                                                                         <  1:607799/97‑1 (MQ=255)
                                                                   cAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATg                                                                                                                                         >  2:607799/1‑97 (MQ=255)
                                                                   cAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAAc                                                                                    >  1:469843/1‑150 (MQ=255)
                                                                      aaCAGCTATAATTTTATATGGGTGGCATTATAAGACGCTACTATCATTCATTGCTGGCATTAGCACACCTGGCGGGTTGTGATCGGGCCTGTTTTTAATGATACCCTTTCACACTTCTGCCGCAGAACGACTCCCCTCCCCCGGAACAAg                                                                                 <  1:1599024/150‑1 (MQ=255)
                                                                         aGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCTTTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTc                                                                              <  1:3965301/150‑1 (MQ=255)
                                                                         aGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTc                                                                              >  2:1168097/1‑150 (MQ=255)
                                                                          gCGATAATTTTATATTGGTGGCATGATAAGACGCGCATATCTTTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCGGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCg                                                                             <  1:5653252/150‑1 (MQ=255)
                                                                          gCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCg                                                                             <  2:886653/150‑1 (MQ=255)
                                                                           cGATAATTTAATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCgg                                                                            >  1:1006084/1‑150 (MQ=255)
                                                                            gATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGt                                                                           >  1:3887339/1‑150 (MQ=255)
                                                                              tAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGttt                                                                         <  2:3367065/150‑1 (MQ=255)
                                                                                aTTTTATATTGGTGGCATGATACGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCa                                                                       <  1:3040453/150‑1 (MQ=255)
                                                                                aTTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCTGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCa                                                                       <  1:4068074/150‑1 (MQ=255)
                                                                                aTTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCa                                                                       <  1:1435974/150‑1 (MQ=255)
                                                                                aTTTTATAAGGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCa                                                                       <  1:3160426/150‑1 (MQ=255)
                                                                                 ttttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGTTGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCAt                                                                      <  2:5060477/150‑1 (MQ=255)
                                                                                    tataATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaa                                                                   <  2:5858085/150‑1 (MQ=255)
                                                                                    tataATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaa                                                                   <  2:2488223/150‑1 (MQ=255)
                                                                                     ataATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGTCCGGATGTGATCCGGCCTGATGTTAATGATAACCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaaa                                                                  >  1:4788552/1‑150 (MQ=255)
                                                                                             ggCATGATACGCCGCGCCTATCATCCTTTGCAGGCATTCGCACACCAGGCGGGATTTGATCCGGCCTGATTTTAATGATACCCTTTCCCACTTCTTCCGCAGAACGACGCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTccc                                                          <  1:5068600/150‑1 (MQ=255)
                                                                                              gCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCg                                                         >  1:2875297/1‑150 (MQ=255)
                                                                                                 tGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATTTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCtt                                                      <  1:5436658/150‑1 (MQ=255)
                                                                                                  gATAAGACGCGACTATCAGTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTg                                                     <  2:3133995/150‑1 (MQ=255)
                                                                                                   aTAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGa                                                    <  2:2235040/150‑1 (MQ=255)
                                                                                                     aaGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGAcc                                                  >  1:5534427/1‑150 (MQ=255)
                                                                                                      aGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGAccc                                                 <  2:5131156/150‑1 (MQ=255)
                                                                                                       gACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGAGCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGAcccc                                                <  2:1064175/150‑1 (MQ=255)
                                                                                                        aCGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGACCAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCg                                               >  2:4688635/1‑150 (MQ=255)
                                                                                                               tATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGt                                        >  2:4264320/1‑150 (MQ=255)
                                                                                                                 tcattcatTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCa                                      <  1:2709848/150‑1 (MQ=255)
                                                                                                                 tcattcatTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCa                                      >  1:4018492/1‑150 (MQ=255)
                                                                                                                 tcattcatTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCa                                      <  1:1133077/150‑1 (MQ=255)
                                                                                                                 tcattcatTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCa                                      <  1:5175380/150‑1 (MQ=255)
                                                                                                                     tcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATAcc                                  <  2:5212778/150‑1 (MQ=255)
                                                                                                                       attGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCaa                                >  1:529930/1‑150 (MQ=255)
                                                                                                                        ttGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAAc                               <  1:4450762/150‑1 (MQ=255)
                                                                                                                           cAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGac                            >  2:3971842/1‑150 (MQ=255)
                                                                                                                           cAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGac                            >  1:3220339/1‑150 (MQ=255)
                                                                                                                             ggCATTAGCACACCAGGCCGGATGTGATCCGGCCTTATGTTAATGATACCCTTTCACACTTCTTCCGCATAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGacac                          <  2:2802755/150‑1 (MQ=255)
                                                                                                                              gCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACt                         <  2:1450769/150‑1 (MQ=255)
                                                                                                                                 ttAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCaa                      >  2:4319483/1‑150 (MQ=255)
                                                                                                                                  tAGCACACCAGGCGGGTTGTGATCCGGCCTTTTTTTATTTATACCCTTTCACACTTCTTCCGAAGAACGACTCCCCTCCCCCGTAACAAGCTCGGTTTAATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAAc                     <  1:2023549/150‑1 (MQ=255)
                                                                                                                                  attaacatCAGGCCGGATCACATCCGGCCTGGTGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAAc                     >  1:236159/5‑150 (MQ=255)
                                                                                                                                    taacatCAGGCCGGATCACATCCGGCCTGGTGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCa                   >  1:5912052/3‑150 (MQ=255)
                                                                                                                                       cacCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATAc                <  2:3475896/150‑1 (MQ=255)
                                                                                                                                         tcAGGCCGGATCACATCCGGCCTGGTGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCg              >  2:3710666/2‑150 (MQ=255)
                                                                                                                                          cAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGc             >  2:1961782/1‑150 (MQ=255)
                                                                                                                                            ggCCGGATGTGATCCGGCCTTATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTg           <  1:5732189/150‑1 (MQ=255)
                                                                                                                                            ggCCGGATCACATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTg           >  2:850484/1‑150 (MQ=255)
                                                                                                                                             gCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTgg          >  1:3662514/1‑150 (MQ=255)
                                                                                                                                              ccGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTGGa         <  1:4945389/150‑1 (MQ=255)
                                                                                                                                              ccGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTGGa         <  2:112590/150‑1 (MQ=255)
                                                                                                                                               cGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTGGAg        >  1:3453234/1‑150 (MQ=255)
                                                                                                                                                 gttgttATCCGGCCTGATTTTAATGATACCATTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTGGAGTa      <  1:1054325/148‑1 (MQ=255)
                                                                                                                                                 gATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTGGAGTa      >  2:4947631/1‑150 (MQ=255)
                                                                                                                                                   tgtgATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTGGAGTATc    <  1:484600/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTGGAGTATCCA  >  NC_000913/2028010‑2028308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: