Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,028,160 G→C 5.4% intergenic (‑143/+28) yedQ ← / ← yodC putative membrane‑anchored diguanylate cyclase/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,028,1600GC 5.4% 1125.6 / 44.4 315intergenic (‑143/+28)yedQ/yodCputative membrane‑anchored diguanylate cyclase/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (142/154);  new base C (13/4);  total (155/160)
Fisher's exact test for biased strand distribution p-value = 2.54e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.40e-01

Only 100 of 325 total aligned reads displayed.

GCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTGGAGTATCCA  >  NC_000913/2028011‑2028308
                                                                                                                                                     |                                                                                                                                                      
gctgcACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGAtgtg                                                                                                                                                        >  2:2115129/1‑150 (MQ=255)
gctgcACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGAtgtg                                                                                                                                                        <  2:1441233/150‑1 (MQ=255)
   gcACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATc                                                                                                                                                     <  1:3987608/150‑1 (MQ=255)
   gcACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATc                                                                                                                                                     >  2:253153/1‑150 (MQ=255)
   gcACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATc                                                                                                                                                     >  1:4448809/1‑150 (MQ=255)
      cccTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCgg                                                                                                                                                  <  2:4467298/150‑1 (MQ=255)
       ccTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGc                                                                                                                                                 >  2:3452492/1‑150 (MQ=255)
       ccTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGc                                                                                                                                                 >  2:1532784/1‑150 (MQ=255)
       ccTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGc                                                                                                                                                 <  2:5172117/150‑1 (MQ=255)
         tGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATATTTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCt                                                                                                                                               <  1:5700825/150‑1 (MQ=255)
         tGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCt                                                                                                                                               >  1:2802755/1‑150 (MQ=255)
         tGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCt                                                                                                                                               <  2:1790503/150‑1 (MQ=255)
           acgacTCCCACCCTTCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGa                                                                                                                                             <  2:3603225/150‑1 (MQ=255)
           acgacTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGa                                                                                                                                             <  1:5642456/150‑1 (MQ=255)
              acTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGt                                                                                                                                          >  1:754715/1‑150 (MQ=255)
               cTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGt‑‑t                                                                                                                                       <  1:1293709/150‑1 (MQ=255)
                tCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑Ta                                                                                                                                      >  1:1500700/1‑150 (MQ=255)
                tCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑Ta                                                                                                                                      >  1:3731859/1‑150 (MQ=255)
                tCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑Ta                                                                                                                                      >  1:5820124/1‑150 (MQ=255)
                tCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑Ta                                                                                                                                      >  1:5296055/1‑150 (MQ=255)
                 cccAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACAGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑Taa                                                                                                                                     >  1:3554267/1‑150 (MQ=255)
                  cccaCCCGCTTGTTGATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAAt                                                                                                                                    <  2:5424599/147‑1 (MQ=255)
                   cAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATg                                                                                                                                   >  1:500476/1‑150 (MQ=255)
                   cAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATg                                                                                                                                   >  1:1922060/1‑150 (MQ=255)
                   cAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATg                                                                                                                                   >  1:563777/1‑150 (MQ=255)
                   cAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATg                                                                                                                                   >  2:5773764/1‑150 (MQ=255)
                    aaCCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGa                                                                                                                                  >  1:2189726/1‑150 (MQ=255)
                        tGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATAcc                                                                                                                              >  2:64640/1‑150 (MQ=255)
                        tGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATAcc                                                                                                                              <  1:2551568/150‑1 (MQ=255)
                         gCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATAccc                                                                                                                             >  1:4224012/1‑150 (MQ=255)
                         gCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCTGgtgtgc                                                                                                                                        >  2:49443/1‑139 (MQ=255)
                           ttttttATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCtt                                                                                                                           >  2:2712497/1‑150 (MQ=255)
                            tttttATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATCCCCttg                                                                                                                          >  1:5383021/1‑149 (MQ=255)
                                 aTGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCacac                                                                                                                     >  1:2116987/1‑150 (MQ=255)
                                  tGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACAct                                                                                                                    >  2:3053083/1‑150 (MQ=255)
                                      tCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACActtctt                                                                                                                >  1:2594126/1‑150 (MQ=255)
                                         ggCATAACCTGGCGATAGCGTACCGCAAAACATCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCg                                                                                                             <  1:2232376/150‑1 (MQ=255)
                                           cATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCa                                                                                                           <  1:5248155/150‑1 (MQ=255)
                                              aaCCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGaa                                                                                                        <  2:2410540/150‑1 (MQ=255)
                                                           cGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGActcccctccc                                                                                           >  2:1473933/1‑150 (MQ=255)
                                                            gTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACtcccctcccc                                                                                          >  2:4225951/1‑150 (MQ=255)
                                                             tACCGCAAAACAGCGATAATTTTATACTGGTGGCGTGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTccccc                                                                                         <  2:2825441/150‑1 (MQ=255)
                                                             tACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTccccc                                                                                         >  2:5883164/1‑150 (MQ=255)
                                                                  cAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAAc                                                                                    >  1:4839488/1‑150 (MQ=255)
                                                                  cAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAAc                                                                                    >  1:469843/1‑150 (MQ=255)
                                                                  cAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAAc                                                                                    >  1:365412/1‑150 (MQ=255)
                                                                     aaCAGCTATAATTTTATATGGGTGGCATTATAAGACGCTACTATCATTCATTGCTGGCATTAGCACACCTGGCGGGTTGTGATCGGGCCTGTTTT‑‑TAATGATACCCTTTCACACTTCTGCCGCAGAACGACTCCCCTCCCCCGGAACAAg                                                                                 <  1:1599024/150‑1 (MQ=255)
                                                                        aGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTc                                                                              >  2:1134506/1‑150 (MQ=255)
                                                                        aGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTc                                                                              >  1:5996892/1‑150 (MQ=255)
                                                                         gCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCg                                                                             <  2:886653/150‑1 (MQ=255)
                                                                          cGATAATTTAATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCgg                                                                            >  1:1006084/1‑150 (MQ=255)
                                                                           gATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGt                                                                           >  1:3887339/1‑150 (MQ=255)
                                                                             tAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGttt                                                                         <  2:3367065/150‑1 (MQ=255)
                                                                               aTTTTATATTGGTGGCATGATACGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCa                                                                       <  1:3040453/150‑1 (MQ=255)
                                                                               aTTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCTGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCa                                                                       <  1:4068074/150‑1 (MQ=255)
                                                                               aTTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCa                                                                       <  1:1435974/150‑1 (MQ=255)
                                                                               aTTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCa                                                                       <  2:3966131/150‑1 (MQ=255)
                                                                               aTTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCa                                                                       <  1:4875016/150‑1 (MQ=255)
                                                                                ttttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCAt                                                                      <  1:5773764/150‑1 (MQ=255)
                                                                                   tataATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaa                                                                   <  1:5890058/150‑1 (MQ=255)
                                                                                   tataATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaa                                                                   <  2:5858085/150‑1 (MQ=255)
                                                                                   tataATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaa                                                                   <  2:2488223/150‑1 (MQ=255)
                                                                                    ataATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGTCCGGATGTGATCCGGCCTGATGT‑‑TAATGATAACCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaaa                                                                  >  1:4788552/1‑150 (MQ=255)
                                                                                        tggtggCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCt                                                              >  1:5067341/1‑150 (MQ=255)
                                                                                             gCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCg                                                         >  1:2875297/1‑150 (MQ=255)
                                                                                                tGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCtt                                                      <  1:3146575/150‑1 (MQ=255)
                                                                                                 gATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTg                                                     >  2:1049246/1‑150 (MQ=255)
                                                                                                  aTAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGa                                                    <  2:2235040/150‑1 (MQ=255)
                                                                                                    aaGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGAcc                                                  >  1:5534425/1‑150 (MQ=255)
                                                                                                     aGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGAccc                                                 <  2:5131156/150‑1 (MQ=255)
                                                                                                      gACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGAcccc                                                >  2:3786667/1‑150 (MQ=255)
                                                                                                       aCGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGACCAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCg                                               >  2:4688635/1‑150 (MQ=255)
                                                                                                              tATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGt                                        >  2:5065882/1‑150 (MQ=255)
                                                                                                              tATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGt                                        <  1:270016/150‑1 (MQ=255)
                                                                                                              tATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGt                                        >  2:4264320/1‑150 (MQ=255)
                                                                                                                tcattcatTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCa                                      <  1:1133077/150‑1 (MQ=255)
                                                                                                                tcattcatTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCGTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCa                                      <  2:1351893/150‑1 (MQ=255)
                                                                                                                   ttcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATAc                                   <  2:25986/150‑1 (MQ=255)
                                                                                                                    tcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaaaa                                                                 >  2:3527733/1‑119 (MQ=255)
                                                                                                                    tcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATAcc                                  <  2:5212778/150‑1 (MQ=255)
                                                                                                                       ttGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAAc                               <  1:3964849/150‑1 (MQ=255)
                                                                                                                       ttGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAAc                               <  2:4803905/150‑1 (MQ=255)
                                                                                                                          cAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGac                            >  1:3220339/1‑150 (MQ=255)
                                                                                                                            ggCATTAGCACACCAGGCCGGATGTGATCCGGCCTTATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCATAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGacac                          <  2:2802755/150‑1 (MQ=255)
                                                                                                                               aTTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGTCTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCa                       >  1:5099864/1‑150 (MQ=255)
                                                                                                                                ttAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCaa                      >  2:4319312/1‑150 (MQ=255)
                                                                                                                                 tAGCACACCAGGCGGGTTGTGATCCGGCCTTTTTT‑‑TATTTATACCCTTTCACACTTCTTCCGAAGAACGACTCCCCTCCCCCGTAACAAGCTCGGTTTAATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAAc                     <  1:2023549/150‑1 (MQ=255)
                                                                                                                                 attaacatCAGGCCGGATCACATCCGGCCTGGTGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAAc                     >  1:236159/5‑150 (MQ=255)
                                                                                                                                      cacCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATAc                <  2:3475896/150‑1 (MQ=255)
                                                                                                                                        tcAGGCCGGATCACATCCGGCCTGGTGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCg              >  2:3710666/2‑150 (MQ=255)
                                                                                                                                         cAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGc             >  2:1961782/1‑150 (MQ=255)
                                                                                                                                           ggCCGGATCACATCCGGCCTGGTGTGCTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGc             >  2:639751/1‑150 (MQ=255)
                                                                                                                                           ggCCGGATCACATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTg           >  2:850484/1‑150 (MQ=255)
                                                                                                                                             ccGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTGGa         <  2:112460/150‑1 (MQ=255)
                                                                                                                                             ccGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTGGa         <  1:4945389/150‑1 (MQ=255)
                                                                                                                                             ccGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTGGa         <  2:2350300/150‑1 (MQ=255)
                                                                                                                                              cGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTGGAg        >  1:3453234/1‑150 (MQ=255)
                                                                                                                                                gttgttATCCGGCCTGATTT‑‑TAATGATACCATTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTGGAGTa      <  1:1054325/148‑1 (MQ=255)
                                                                                                                                                gATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTGGAGTa      >  2:4947631/1‑150 (MQ=255)
                                                                                                                                                  tgtgATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTGGAGTATc    <  1:4600850/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GCTGCACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGT‑‑TAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTGGAGTATCCA  >  NC_000913/2028011‑2028308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: