Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,245 G→T 14.1% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2450GT14.1% 710.8 / 11.4 307intergenic (‑41/+267)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (116/128);  new base T (36/20);  total (153/154)
Fisher's exact test for biased strand distribution p-value = 2.65e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 314 total aligned reads displayed.

TTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  NC_000913/2177096‑2177394
                                                                                                                                                     |                                                                                                                                                     
tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATg                                                                                                                                                       >  2:3367191/1‑150 (MQ=255)
tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATg                                                                                                                                                       >  2:707850/1‑150 (MQ=255)
    tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAat                                                                                                                                                   >  2:2780391/1‑150 (MQ=255)
    tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGGTTTCGATTTCAAAATATAATGAAat                                                                                                                                                   >  2:1793494/1‑150 (MQ=255)
     accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaaaat                                                                                                                                                  >  2:5468085/1‑148 (MQ=255)
     accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatt                                                                                                                                                  >  1:2932903/1‑150 (MQ=255)
       caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattat                                                                                                                                                <  2:803803/150‑1 (MQ=255)
        accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                               <  1:1979792/150‑1 (MQ=255)
        accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                               <  2:1308221/150‑1 (MQ=255)
        accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                               <  1:4972718/150‑1 (MQ=255)
         ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                                              <  2:2976585/150‑1 (MQ=255)
         caacTTGCATCTTTTAGAGATTGTGAATATTGAATGCCGGAACCGCATAAGCGCCGCGCTGTTCGTTGTTGTGAATCTGCTTTGTCGATAACACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATTATGAAATTAttt                                                                                                                                              <  1:3198662/148‑1 (MQ=255)
            cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtt                                                                                                                                           <  2:1703859/150‑1 (MQ=255)
             ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGttt                                                                                                                                          <  1:4794520/150‑1 (MQ=255)
             ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATTAAATTATtttttt                                                                                                                                          >  1:1703243/1‑150 (MQ=255)
             ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGCCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATTAAATTATtttttt                                                                                                                                          >  1:2269495/1‑150 (MQ=255)
                  tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaaa                                                                                                                                     <  1:3342679/150‑1 (MQ=255)
                    gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGAAATTATTTGTTTTTAAata                                                                                                                                   >  1:849949/1‑150 (MQ=255)
                      ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                                 <  2:5324266/150‑1 (MQ=255)
                       tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAatatag                                                                                                                                >  1:3237016/1‑148 (MQ=255)
                        cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                               <  2:1463866/150‑1 (MQ=255)
                        cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                               <  1:1638708/150‑1 (MQ=255)
                          agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCgaga                                                                                                                             >  2:4943099/1‑150 (MQ=255)
                              tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAc                                                                                                                         >  2:3949499/1‑150 (MQ=255)
                               gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTGAAAATATTATTTAATTATTTGTTTTTTAATATGGAGATaaag                                                                                                                        >  1:436307/1‑148 (MQ=255)
                                tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAATTAAAAATAAAATGAAATTATTTTTTTTTAAATATAGAGATTACGa                                                                                                                       >  1:1500542/1‑150 (MQ=255)
                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                                      <  2:589268/150‑1 (MQ=255)
                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                                      <  1:2370874/150‑1 (MQ=255)
                                   atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                                    <  2:1673165/150‑1 (MQ=255)
                                   atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                                    <  2:1673242/150‑1 (MQ=255)
                                    tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAATGTAATTATTTGTGTTTAAATATAGAGATAACGATcac                                                                                                                   >  1:3493652/1‑150 (MQ=255)
                                     atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcaca                                                                                                                  <  1:2733088/150‑1 (MQ=255)
                                         aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGTTTTGAAAATATATTGTAAATATTTTTTTTTGAATATATGGGTAAACATCACaaaaa                                                                                                              >  1:2848532/1‑150 (MQ=255)
                                         aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                              >  2:2872726/1‑150 (MQ=255)
                                         aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                              <  2:1842190/150‑1 (MQ=255)
                                          aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATAACaaaaaa                                                                                                             >  2:3165980/1‑149 (MQ=255)
                                            gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                           <  2:3396321/150‑1 (MQ=255)
                                            gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGATTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                           <  2:2465227/150‑1 (MQ=255)
                                            gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAATTATTTTTTTTTTAATATCGAGATAACGATCAAAAAAAAGa                                                                                                           >  2:2307942/1‑150 (MQ=255)
                                              cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAAAATCGAGATAACGATAACAAAAACGACa                                                                                                         >  2:211320/1‑150 (MQ=255)
                                               ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATGTCAAAATATAATGAAATTATTTGTGTTTTAATATCGTGAGACCGATCACCAAAACGACaa                                                                                                        >  2:3980257/1‑150 (MQ=255)
                                                      cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTAAATTATTTTTTTTTAAATATAGAGATAAAGATCAAAAAAAAGACAAAATGaaa                                                                                                 >  1:128007/1‑150 (MQ=255)
                                                       aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAACGACAATAAGaaaa                                                                                                >  2:1896395/1‑150 (MQ=255)
                                                          aCCGCCGCGCTGGGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATAACCTGACGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAatta                                                                                             <  2:4295624/150‑1 (MQ=255)
                                                               cgcgCTGTTCGTTGTTCAGCATCTGCTTTGTCGATACCCCGAACATTTTCATATCCTGACGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                                        <  2:3410972/150‑1 (MQ=255)
                                                               cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAAAGAAAATAATTTTTTTTAAATATAGAGAAAACGATCACAAAAACGACAAAATGAAAATTAATCga                                                                                        >  2:5283592/1‑150 (MQ=255)
                                                                  gcTGTGCGTTTTTCATAATCTGTTTTGTCGATACCACTTAAATTTTCATCCCCTGTGTTTTTTTTTCTATTTCAAAATATAATTAATTTATTTTTTTTAAAATTTCTATATAACGAACAAAAAAACGACAATATGAAAATTATTCgagga                                                                                     <  1:5468085/150‑1 (MQ=255)
                                                                  gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACTTACATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTTTTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgagga                                                                                     <  2:1137801/150‑1 (MQ=255)
                                                                    tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAAAAATGAAATTATTTTTTTTTAAATATCGAGATAAAGATAACAAAAAAGACAAAAAGAAAATTATTAGAGGAGt                                                                                   >  2:1211953/1‑150 (MQ=255)
                                                                        cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                               <  2:5162688/150‑1 (MQ=255)
                                                                         gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAg                                                                              <  2:652036/150‑1 (MQ=255)
                                                                          ttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAgg                                                                             <  2:5397763/150‑1 (MQ=255)
                                                                          gtgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAgg                                                                             <  2:3324676/149‑1 (MQ=255)
                                                                               cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAAATATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAAAATGAAAATTATTCGAGGAGTGAAAGGCaaaa                                                                        >  2:3622426/1‑150 (MQ=255)
                                                                                 gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAAAAAAATTAATTAATTTTTTTTAAATATAGAGATAAAGAAAACAAAAAAAACAAAATAAAAATTAATAGGGGATTGAAAGGCaaaaaa                                                                      >  2:5620572/1‑150 (MQ=255)
                                                                                  cATCTGCTTTGTCGATACCACTTACATTTACATATCCTGTCTTTTTTTTTCGATTTCAAAATATAATTAATTTATTTTTTTTTAAATTTCGATATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                                     <  1:2521099/150‑1 (MQ=255)
                                                                                   ttCTGCTTTGTCTAGACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                    <  2:3880856/149‑1 (MQ=255)
                                                                                   aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                    <  2:526322/150‑1 (MQ=255)
                                                                                    tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGCGGAGTGAAAGGCAAAAAAACg                                                                   >  2:5395975/1‑150 (MQ=255)
                                                                                    tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACg                                                                   <  2:3243764/150‑1 (MQ=255)
                                                                                        cTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCt                                                               >  2:3277287/1‑150 (MQ=255)
                                                                                        cTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAAATATTTTTTTTTAAATATAGAGATAAAGAACACAAAAAAGACAAAATTAAAATTATTAGATGAGTGAAAGGCAAAAAAAAGGCCt                                                               >  2:2665942/1‑150 (MQ=255)
                                                                                         tttGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTAATTTTTTTTAAATATCCAGATAACCATCACAAAAAAGACAAAAATAAAATTATTAGAGGAGTGAAATGCAAAAAAAAGGCCTc                                                              >  2:1665250/1‑150 (MQ=255)
                                                                                         tttGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAAGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTc                                                              >  2:892725/1‑150 (MQ=255)
                                                                                          ttGTCGATACCACGTACATTTTCATATCCTGTCGTGTGGTTTCGATTTCAAAATATAAAGAAAATAATTGTTTTTAAATATCGAGATAACCATCACAAAAACGACAAAATGCACATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTcc                                                             >  2:2153795/1‑150 (MQ=255)
                                                                                          ttGTCCAAACCACTTACAATTTCATATCCTGTCTTTTGTTTTCCATTTCAAAATATAATCAAATTATTTGTTTTAAAATATCGACATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTcc                                                             <  2:2160622/150‑1 (MQ=255)
                                                                                               gATACCACTTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                                        <  2:6002395/150‑1 (MQ=255)
                                                                                                 tACCACTTACTTTTTCATTTCGTGTCGTTTTTTTTCTTTTACAAATTATATTTAATTTTTTTGTTTTTAATTATCGATATACCTAAAACAAAAACGACAATATGAAAATTATTCGTGGAGTGAACGGCAAAAAAACGGCCTCCCGATAgg                                                      <  1:5012557/150‑1 (MQ=255)
                                                                                                 tACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAgg                                                      <  2:5203327/150‑1 (MQ=255)
                                                                                                 tACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAgg                                                      <  2:3814929/150‑1 (MQ=255)
                                                                                                    cACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTGAAAATATAATGAAATTATTTTTTTTTTAATATGGGGTTAAAGTTGACAAAAAAGACAATATGTAAATTATTTGTGGGGTTTAAGGCAAAAAAAAGGCCTCCCGGTAggggg                                                   >  2:2545665/1‑148 (MQ=255)
                                                                                                    cACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                                   <  2:1429646/150‑1 (MQ=255)
                                                                                                     aCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAAGATCACAAAAAAGACAAAATGAAAATTATTCGAGGAGTGTAAGGCAAAAAAAAGGCCTCCCGAGAGGGAAg                                                  >  2:3214307/1‑150 (MQ=255)
                                                                                                      cGTACATTTTCATATCCTGACGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                                 <  2:2384799/150‑1 (MQ=255)
                                                                                                       gTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATAACAAAAACGACAATAAGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCGATAGGGAAGcc                                                >  2:4199854/1‑150 (MQ=255)
                                                                                                               taatatCCTTTGTTTTTTTTACTATTTAAAATTTTAATCAATTTATTTTTTTTTAAATATCTAGAAAACGAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                                        <  1:2564972/148‑1 (MQ=255)
                                                                                                               tCATATCCTGGCGTTTGTTTTCGATTTCAAAATATAATGAAATTTTTTGTTTTTTAATATCTAGATATCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                                        <  2:4701309/150‑1 (MQ=255)
                                                                                                               acATATCCTGTCGTTTTTTTTCGATTTCAAAAAATAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                                        <  1:3864663/149‑1 (MQ=255)
                                                                                                                cATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAAATATTTTTTTTTAAATATAGAGATAACGATAACAAAAAAGACAATATGAAAATTATTAGAGGAGTGAAATGCAAAAAAAAGGCCTCCCGATAGGGGAGCCGTAGCAAAg                                       >  2:105325/1‑150 (MQ=255)
                                                                                                                 atatCCTGTCTTTTTTTTTCTATTTCAAAATATAATTAAATTTTTTTTTTTTAAATATCGATATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGt                                      <  1:5667171/150‑1 (MQ=255)
                                                                                                                  aatCCTGTCTTTTTTTTTCTATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGATATAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTg                                     <  1:3021043/149‑1 (MQ=255)
                                                                                                                    tCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcg                                   >  2:4515633/1‑150 (MQ=255)
                                                                                                                     ccTGTCTTTTTTTTTCTATTACAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                                  <  1:602264/150‑1 (MQ=255)
                                                                                                                     ccTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                                  <  1:3634559/150‑1 (MQ=255)
                                                                                                                     ccTGTCGTTTGTTTTCGATTACAAAATATAATTAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                                  <  1:4918816/150‑1 (MQ=255)
                                                                                                                           gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                            <  2:5497209/150‑1 (MQ=255)
                                                                                                                           gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                            >  2:5870216/1‑150 (MQ=255)
                                                                                                                                ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                       >  1:1850156/1‑150 (MQ=255)
                                                                                                                                 tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                      >  1:5790550/1‑150 (MQ=255)
                                                                                                                                 tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                      >  1:5122877/1‑150 (MQ=255)
                                                                                                                                   tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                    <  1:4424228/150‑1 (MQ=255)
                                                                                                                                        ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc               >  2:5797418/1‑150 (MQ=255)
                                                                                                                                         tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg              <  1:777470/150‑1 (MQ=255)
                                                                                                                                          cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCGCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg             <  1:4225166/150‑1 (MQ=255)
                                                                                                                                           aaaaTATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt            <  2:1078521/150‑1 (MQ=255)
                                                                                                                                           aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg             >  1:5352709/1‑149 (MQ=255)
                                                                                                                                           aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg             <  2:5352709/149‑1 (MQ=255)
                                                                                                                                           aaaaTACAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt            <  2:4090678/150‑1 (MQ=255)
                                                                                                                                            aaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg           >  2:5549547/1‑150 (MQ=255)
                                                                                                                                                    tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa   <  1:1113393/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  NC_000913/2177096‑2177394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: