| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 164,595 | T→G | 11.0% | intergenic (+61/‑135) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 164,595 | 0 | T | G | 11.0% | 369.2 / 15.1 | 133 | intergenic (+61/‑135) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Reads supporting (aligned to +/- strand): ref base T (14/101); new base G (15/2); total (29/104) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.86e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 142 total aligned reads displayed.
AAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATG > NC_000913/164446‑164742 | acgataTGAAGGGGGTTTATCCCAAACATGTCTGGCGGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACTAAAATTTTTTCTTATGTTTGTTTTTCTCCCTCTCCCTGTGGTTTTGGGCGGGGTTTTGGGCATCAGCGCGCACGtt < 2:2343872/145‑1 (MQ=255) agaTGAATGGGCGTTATCCCAAACTTGTCTGGCCGGACGACCCGGAAAAT‑ACTGCACCGACGCGACGGACGAAAATGTATTCTTAAGTTTGTTTTTCTCCCTCTCCCTTTGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcac < 2:3040885/150‑1 (MQ=38) agaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGAAAAT‑ACTGCACCGACGCGACGGACTAAAATTTATTCGTAAGGTTGGTTTTCGCCCTCTCCCTGTGGTTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTtcac < 2:3370062/150‑1 (MQ=255) aTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgcaccc > 2:2643346/1‑150 (MQ=255) gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcgccccc > 2:1906669/1‑148 (MQ=255) aaaGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACGGCACCGACGCGACGGACGAAAAAGTTTTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTtcaccctca < 2:3540528/150‑1 (MQ=255) cGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCTTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTaa < 2:3908165/150‑1 (MQ=255) gTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTGCGCCCACACCCAAAc > 1:331383/1‑150 (MQ=38) gTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGTGGGCCCCCACCCCaaa > 1:2676431/1‑149 (MQ=38) ttATCCAAAACTTGTCTGGCGGGACGACCCGGAAAAT‑CCTGCACCGACGCGAGGGACGAAAATTTTTTCGTACGGTTGTTTTTCTCCCCCCCCGGGGGGTTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc < 2:5023776/150‑1 (MQ=255) cccAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctct > 1:4799987/1‑150 (MQ=255) ccAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCACCCAAACCctcta > 2:491047/1‑149 (MQ=255) ccAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGGAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCGCACCCACAcccacac > 2:5325887/1‑146 (MQ=38) cAAACCTGGCTGGCCGGACGACCCGGCAAAT‑ACTGCCCCGACGCGCCGGACGAAAATGTATGGGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc < 2:3441073/150‑1 (MQ=37) cAAACATGTCTGGCCGGACGACCCGGCAAAT‑CCTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc < 2:4872175/150‑1 (MQ=255) cAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc < 2:3441600/150‑1 (MQ=255) aaaCATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGGCCCCCCACCCCAACCCACAccc > 1:358372/1‑150 (MQ=37) aCATGTCTGGCGGGACGACCCGGAAATT‑ACTGCCCCGACGCGACGGACTAAAATGTATTCTTACGGTTGGTTTTCTCCCCCTCCCTGTGGGTGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTc < 2:5657769/150‑1 (MQ=255) tttagtGGCCGGCCCCCCGGGCAAAT‑CCTGCCCCGACGCCCCGTACGAAAAAGTTTTCTTATGTTGGGTTTTCCCCCCCGCCCGGGGGGTGTGGGCGGGGTTTAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 2:5050563/145‑1 (MQ=255) aTGTCTGGCCGGACGCCCCGGCAAAT‑ACTGCCCCGACGCGACGGACGACAAAGTAGTCGTCCGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCaa < 2:4887186/150‑1 (MQ=37) tgtggggccggccGCCCCGGAAAAT‑ACTGCCCCGCGGCGCCGTACTAAAATTTTTTGGTTTGGTTGTTTTTCACCCACTCCGTGGGGGTGTGGGGGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 1:5851939/145‑1 (MQ=255) tGTCTGGCGGGACGACCCGGCAAAT‑CCGGCACCGCCGCGACGGACTAAAATTTTTTCGTTTGGTTGGTTTTCCCCCTCTCCGTGTGGGTTTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:1711122/150‑1 (MQ=255) tGTCGGGCCGGCCGACCCGGAAAAT‑CCTGCCCCGACGCGACGGACTAAAAAGTATTCTTACGGTTGGTTTTCACCCTCCCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:332692/150‑1 (MQ=255) ggtgtGGCCGGACGACCGGGAAAAT‑ACTGCACCGCGGCGACGGACTAAAATGTATTCTTATGTTTGGTTTTCTCCCTCTCCGTGTGGTAGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:1711019/146‑1 (MQ=255) tGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCGAACCCACAACCACAAGGgag > 2:4504978/1‑150 (MQ=38) tGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCACAACCAAACCCACTCCCACAAggggg > 2:4505283/1‑148 (MQ=38) ggCGGGACGCCCCGCCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTACGTTTGGTTTTCGCCCTCACCGGGGGGGCGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgaga < 2:4156635/150‑1 (MQ=255) ggCCGGACGACCCGGCAATT‑ACTGCACGGACGCGACGGACGAAAAAGTTTTGGTAAGGTTGGTTTCCTCCCTCGCCCGGTGGGAGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgaga < 2:4814256/150‑1 (MQ=255) gCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGTACGAAATAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag < 2:4864509/150‑1 (MQ=255) gCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGGCCCCGCACCCCAAACCACGCCCGCAAggggggg > 1:1478369/1‑146 (MQ=37) gCCGGACCCCCCGCAAAAT‑CCTGCACCCACGCGACGGACTAAAAATTTTTCGTATGTTTGGTTTTCCCCCTCTCCCTGGGGGTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGagag < 2:5647137/150‑1 (MQ=255) cccGGACCCCCCGGAAAAT‑ACTGCACCGCCGCGACGCACGAAAAATTATTGTTACGGTTGTTTTTCGCCCACCCCCGGTGGGCGAGGGCCGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGagag < 2:2216801/149‑1 (MQ=255) cgggACTACCGGGAAAAT‑ACTGCACGTCCGCTAGGTACTAAAATTTTTTGTTATGGTTGGTTTTCCCCCTCTCCGTGTGGGTTTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAgg < 1:2888469/148‑1 (MQ=255) ccGGACCACCGGGAAAAT‑ACTGCACCCACGCGAGGGACCAAAATGTTTGCGTACGGTGGGTTTTCACCCACCCCCTGTGGGAGCGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAgg < 2:1597878/150‑1 (MQ=255) cgtacgacCCCTAAAAT‑ATTCCACCTACGCGCCTCACTAAAATTTTTTCTTATGTTTGTTTTTGCCCCTCTCCTTGTGGTTGTGGGCGGGGGTTTGGGCATCAGCGCGCACGTTCACACTCACCCTAACACTCTCACTCAAGGGAGAggg < 2:5054183/147‑1 (MQ=255) ggACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCACCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTAACCCACACCCAAAACCACTCCCACAAGGGAGAgggg > 2:1498538/1‑150 (MQ=38) cggcccccccGCAAATAACTGCCCCGAGGCGAGGGACTAAAATTTTTTCGTACGGTTGGTTTACTCCCACTCCGTGTGGTTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg < 1:3978622/144‑1 (MQ=255) gacgccCCGGAAAAT‑ACGGCCCCGACGCGCCGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCGCGCCCTGTGGTAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa < 2:729266/150‑1 (MQ=38) gacgacCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCCCCCCCCAACCCGCGCCCGCCACGGGGAggggg > 2:5470533/1‑149 (MQ=37) gacgacCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCCCACCCGAACCCACACCCGCACGGGGGAggggg > 2:5470541/1‑149 (MQ=37) gaccacCCGCCAAAT‑ACTGCCCCGCCGCGACGGACGAAACAGTATGCGTAAGGTGGGTTTCCTCCCCCCCCCTGTGGGAGAGGGCCGGGTTGCGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGTGAGAGGGGa < 2:729221/150‑1 (MQ=255) cccGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCACACCCAAAACCACAACCGCAAAGGGGAGGGGACCGaa > 1:3510512/1‑149 (MQ=25) ccGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCACACCCAAAACCAAAACCACAAGGGGGAGGGGccccaac > 2:3833282/1‑146 (MQ=25) cGGCAAAT‑ACTGCACCGACGCGACGGACGACAAAGTATTCGTCAGGGTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGCACCCGCACCCCAACCCGCGCCCCCCCGGGCGCGGGGAccccgcg > 2:2491752/1‑145 (MQ=21) cGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCCCCCGCACCCAAACCCACACCCACAACGGAGCGGGGGCCGAACg > 1:5740558/1‑150 (MQ=25) gCAAAT‑ACTGCCCCGACGCGACGGACCAAAAAGTTTTCTTAAGGTGGGTTTTCACCCTCTCCCTGGGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg < 1:4356040/150‑1 (MQ=255) aaaaT‑CCTCCACCGACGCGACGGACTAAAATTTATTCTTCTGTTTGGTTTTCTCCCTCTCCGTGTGGGTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGc < 1:2872994/149‑1 (MQ=255) aaaT‑ACTTCACCGAGGCGCCTGACTAAAATTTTTTCTTATGTTTGTTTTTCTCCATACCCCTGTGGGAGTGGGCCGGGTTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca < 2:200355/150‑1 (MQ=255) cTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCGCACCCGAAACCACACCCTCCCGGGAGCGGGGGCCGGTCGCGCCCCAAt > 1:5880434/1‑150 (MQ=25) gCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGGCCCACACCCAAAACCAAAACCACAAAGGGGAGGGGGCCCCACGAGcacacaaaa > 2:4702842/1‑145 (MQ=21) cACCGCCGCGACGGACGAAAATGTTTTCTTATGGTTGGTTTTCGCCCTCTCCGGGTGGGAGTGGGCGGGGGGGTGGGCCTCCGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 1:1362674/150‑1 (MQ=255) cACCGACGCGACGGACGAAAAAGTATTCGTATGGTTGGTTTTCCCCCTCTCCCTGTGGGAGCGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 1:853925/150‑1 (MQ=255) ccgccgCGACGCACTAAAAAGTTTTCGTACGGTGGGTTTACGCCCTCCCCCTGGGGGTGCGGGCGGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:2246253/150‑1 (MQ=255) ccGACGCGACGTACGAAAAAGTATGCGTACGTTTGGTTTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 1:3729605/150‑1 (MQ=38) ccGACGCGACGGACTAAAATGTTTTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:3654856/150‑1 (MQ=38) ccGACGCGACGGACGAAAAATTATTCTTAAGGTTGGTTTTCTCCCTCGCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGTGAGAGGGGACCGATCGAGCACAAATTTTga < 2:1459364/150‑1 (MQ=38) ccGACGCGACGGACGAAAAAGTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:4451034/150‑1 (MQ=255) ggccgcgacgGACCAAATTGTAGTCGTACGGTTGGTTTTCCCCCTCTCCGTGGGGGAGAGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgag < 1:4757748/147‑1 (MQ=255) ggacgAAAATGTATTCGTATGTTTGGTTTCCGCCCTCTCCCTGTGGTAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGTGACCGATCGAGCACAAATTTTGAGAGATAtctt < 2:4235759/150‑1 (MQ=38) gAACAAGTATTCGTACGGGTGGTTTTCTCCCTCGCCCTGGGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc < 2:1967817/150‑1 (MQ=255) gAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCACACCCCAACCAACACACACAAAGGGAAGGGGGCCCAGCCAGCCCAACATTTTAGAGATAtcaaataa > 1:4189009/1‑144 (MQ=12) aattATTCGGTAGGTTGGTTTGCCCCCTCACCCTGGGGTTGCGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc < 2:4111279/147‑1 (MQ=255) gTATTGGTTGGGTTGGTGTTCTCCCTCGCCGGGGGGGTGTGGGCGGGGGTGGGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 2:609880/150‑1 (MQ=255) gTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 2:3576614/150‑1 (MQ=255) tggTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCTCCCTCACGCTAACCCTCTCCCTGACGGGAGAGGGGCCCGATGGAGCACAACTTTTGAGAGATCTCGTCTTCTGTGTTGTaa < 2:3976378/148‑1 (MQ=25) gTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCTGGGGTGAGGGCATCAGCGCGCACGTTCAACCGCACCCCAAACCGCGACCCCAAGGGAGAGGGGGCCGCGCGCGCCCAAAATTTGTGAGCTATCGTCTTCTGTCGTGTGACa > 2:3495373/1‑150 (MQ=16) cgtttggttTCCCCCCCCCCCCGGGGGGAGTGGGCCGGGGTGCGGGCATCCGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 1:1633985/147‑1 (MQ=255) cggtgggttTTCGCCCGCGCCGTGTGGGCGCGGGCGGGGGGGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 2:1704955/149‑1 (MQ=255) gttgtttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaaga < 1:4505283/150‑1 (MQ=255) gttggttTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaaga < 1:4505439/150‑1 (MQ=255) tggttTACTCCCCCCCCCTGGGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAAc < 1:1868395/150‑1 (MQ=255) ggttTTCGCCATCTCCGGGTGGGTGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACa < 1:2760826/150‑1 (MQ=255) tctccctctcccGGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGaaaa < 2:1446265/150‑1 (MQ=255) cccctccccttGTGGTTGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATc < 1:512413/149‑1 (MQ=255) cccctcaccccGGGGGAGCGGCCCGGGGCGCGGCCATCAGCGCGCACCTGCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTGTGAGAGAGATCTTCCTCTGTCTTGTAACAGAAGAACAGAAAATc < 2:243296/149‑1 (MQ=255) ccctctccctGTGGGAGAGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTACCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTATTGTAACAGAAGAACAGAAAATCg < 2:3707100/150‑1 (MQ=255) cctctccgtGTGGGTGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCgg < 1:3277824/150‑1 (MQ=255) ctcgccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCggg < 2:3592649/150‑1 (MQ=255) ctccccctGTGGGAGAGGCCCGGGGTGAGGGCATCAGCGCGCACGTACACCCTCACCCTAACCCACTCCCTCAAGGGAGAGGGGACCGAACGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCggg < 2:5482463/150‑1 (MQ=255) cctGTGGGAGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCtttt < 2:5817471/150‑1 (MQ=255) tgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCACCCAAACCCACACCCGCAAGGGGGTGGGGGCCCATCGAGCACAAAATTTTAGATATATATTAATATGTCTTTTAACAGAAGAAAAGAAAAAAGGGCGTTTtcgc > 2:3007351/1‑150 (MQ=255) ggggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc < 1:1386496/149‑1 (MQ=255) ggAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt < 2:2375443/150‑1 (MQ=255) ggAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCTCACCCAAAACCAAGACCAAAAGGGGGGGGGGGCCCGGCGCGGACAAAATTTGGGAGAAATGTTGTTGTGTGTTTTTACAGAAGAACAGAAAATCGGGCgttttggccg > 2:3368834/1‑144 (MQ=255) gggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAata < 2:4376385/150‑1 (MQ=255) ggCCGGGGTGAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAatat < 1:1906669/150‑1 (MQ=255) cgggggggTGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACATAAAATCGGGCTTTTGCGCCTGAATATTg < 1:734269/148‑1 (MQ=255) cGGGGTGAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGc < 1:3324446/150‑1 (MQ=255) ggggTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:5550300/150‑1 (MQ=255) ggggTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:1950152/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:5995372/150‑1 (MQ=255) aGGGCATCAGCGCGCCCGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaa < 1:1364337/150‑1 (MQ=255) aGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCACTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaa < 2:4799987/150‑1 (MQ=255) gggCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCGGAATATTGCGGAGaaaa < 2:2431823/150‑1 (MQ=255) gggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaa < 2:4623265/150‑1 (MQ=255) gcctCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAg < 2:2565258/147‑1 (MQ=255) aTCAGCGCGCACGTTCACCCACACCCAAAACCACAACCAAAAGGGAGAGGGGACCCATCGAGCACAAAATTTGAGAGAAATAATATTATGTCTTGTAAAAGAAGAACAGAAAAAAGGGCGTTTTCGCCGGAATAATTCGGAGAAAAAACa > 2:3498292/1‑150 (MQ=255) tCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGAACGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCAt < 2:5250969/150‑1 (MQ=255) gcgcACGTTCACCCTCACCCTAACCCTCTCCCTCTAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCg < 1:3694863/150‑1 (MQ=255) gTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTATTGTAACACAAGAACAGAAAATCGGGCTTTTGCGCCAGAATATTGCGGAGAAAAAGCATGGCCGGGAATg > 2:4903144/1‑150 (MQ=255) | AAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAAT‑ACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATG > NC_000913/164446‑164742 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |