Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,304,680 T→C 7.2% intergenic (+287/+428) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,304,6800TC 7.2% 433.6 / 14.1 175intergenic (+287/+428)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base T (52/102);  new base C (4/8);  total (60/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 183 total aligned reads displayed.

TAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGC  >  NC_000913/2304531‑2304829
                                                                                                                                                     |                                                                                                                                                     
tAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCATCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCt                                                                                                                                                       <  1:3975566/150‑1 (MQ=255)
    tCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACg                                                                                                                                                   >  1:2972367/1‑150 (MQ=39)
    tCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACg                                                                                                                                                   >  2:2044304/1‑150 (MQ=39)
      aGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAAGCCGCATCCGACATCAAACGcc                                                                                                                                                 >  1:4632041/1‑150 (MQ=255)
      aGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCGACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGcc                                                                                                                                                 >  1:836141/1‑150 (MQ=38)
      aGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGGCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGcc                                                                                                                                                 >  1:3002151/1‑150 (MQ=38)
       gACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGccc                                                                                                                                                >  1:2321/1‑150 (MQ=255)
        aCAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCa                                                                                                                                               >  2:3346536/1‑150 (MQ=35)
        aCAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCa                                                                                                                                               >  2:1689904/1‑150 (MQ=35)
         cAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCaa                                                                                                                                              <  1:3718185/150‑1 (MQ=35)
             gCGGTCACGCCGCATCCGACATCCAACGCCCGAGCGGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCTTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCg                                                                                                                                          <  1:263248/150‑1 (MQ=21)
             gCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCGAGCCg                                                                                                                                          >  2:2159379/1‑150 (MQ=21)
             gCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCg                                                                                                                                          >  1:871215/1‑150 (MQ=34)
             gCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCg                                                                                                                                          >  2:3388375/1‑150 (MQ=34)
             gCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCg                                                                                                                                          <  1:340816/150‑1 (MQ=34)
              cGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCgg                                                                                                                                         <  2:229667/150‑1 (MQ=34)
               ggTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATATGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGt                                                                                                                                        <  1:5448518/150‑1 (MQ=21)
                gTCTCGCCGCATCCGACATCCATCGCGCGAGCGGGTTGCCTTATGCTACTCTGGCGCGTCTTATCTGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGtt                                                                                                                                       <  1:4366866/150‑1 (MQ=11)
                gTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGtt                                                                                                                                       <  1:3924831/150‑1 (MQ=34)
                gTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGtt                                                                                                                                       <  1:1976979/150‑1 (MQ=34)
                 tCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCAGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAAAGCCCAAGCCGGTTg                                                                                                                                      >  1:1016627/1‑150 (MQ=18)
                  cACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATTCGCCGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTGGGAGAAGGCGGTGACGCCGCCACCGACAGCCAACGCCCGAGCCGCTTGc                                                                                                                                     >  1:1035251/1‑150 (MQ=11)
                  cACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTGCGCCGCATCCGACATCTAACGCCCAAGCCGGTTGc                                                                                                                                     <  1:4930092/150‑1 (MQ=17)
                   aCGCCGCATCCGACATCCTACGCCCGAGGGGGTTGCGTTATTCTACGCTGGCTCGTCGTATCTGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGcc                                                                                                                                    <  1:4419979/150‑1 (MQ=11)
                   aCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGcc                                                                                                                                    <  1:2113819/150‑1 (MQ=32)
                   aCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGcc                                                                                                                                    <  2:2837235/150‑1 (MQ=32)
                    cgccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCTACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCt                                                                                                                                   <  1:4583774/150‑1 (MQ=17)
                     gccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTg                                                                                                                                  <  1:3374809/150‑1 (MQ=32)
                      ccgcATCCGACCTCCACCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGa                                                                                                                                 <  2:4568636/150‑1 (MQ=17)
                      ccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATTCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGa                                                                                                                                 <  2:5730659/150‑1 (MQ=17)
                      ccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGa                                                                                                                                 <  1:4047310/150‑1 (MQ=32)
                       cgcCTCCGACATCCAACGCCCGAGCCGGTTGCCGGATGCGACTCTGGCTCTTCTTATCAGTCATACACCGCTGTGAAGTGGTCCTCCCGTTATGTCTGATAAGGCGGTAACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGAt                                                                                                                                <  2:2238743/150‑1 (MQ=11)
                       cgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGAt                                                                                                                                <  2:4887169/150‑1 (MQ=32)
                        gccgccGACATCCACCGCCCGCCCCGGTTTCCGGATGCGACCCGGGCTCGCCTTATCTGGGCTACACCGCGGTGAAGTGCGGCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATg                                                                                                                               <  2:855672/146‑1 (MQ=11)
                             cGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAGGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACg                                                                                                                          >  2:1807032/1‑150 (MQ=17)
                             cGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACg                                                                                                                          <  1:4702035/150‑1 (MQ=32)
                              gACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGc                                                                                                                         >  2:2521591/1‑150 (MQ=32)
                                  tCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAg                                                                                                                                             <  1:584358/126‑1 (MQ=32)
                                  tCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgc                                                                                                                     >  2:1252463/1‑150 (MQ=32)
                                    ccccGCCCGAGCCGGGTGCCTGCTGCGACGCGGGCGCGTCTTCTCTGGCGTACACCGCTGTGAAGTGCGCCACCCCGTAGGTCGGAGAAGGCGGTGACGCCGCATCCGACATCTAACGCCCCAGCCGGTTGCCTGCTGCGACGCTGgcgc                                                                                                                   <  2:1308036/147‑1 (MQ=11)
                                    caccGCCCGAGCCGGTCGCCCGACGGGTCTCTGTCGCGTCTGAAGAGGGCTAAACCGCCGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTAACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                   <  2:356338/147‑1 (MQ=11)
                                    cAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                   <  2:3525074/150‑1 (MQ=17)
                                    cAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                   >  2:3610722/1‑150 (MQ=32)
                                    cAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                   >  2:9950/1‑150 (MQ=32)
                                     aaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGc                                                                                                                                            >  2:626734/1‑124 (MQ=32)
                                     aaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGc                                                                                                                                            <  1:626684/124‑1 (MQ=32)
                                     aaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGcgcg                                                                                                                  <  1:2521591/150‑1 (MQ=32)
                                     aaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACAGCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGcgcg                                                                                                                  >  2:3579931/1‑150 (MQ=17)
                                      ccGCCCGAGCCGGTTGCCTGATGCGAATCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTGCACCCCGTAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCGAACGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                 <  2:50323/149‑1 (MQ=11)
                                      ccGCCCGAGCCGGTTGCCTGACGCGACGCTGTCGCGTCTTATCATGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCAAACGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                 <  2:50352/149‑1 (MQ=11)
                                      aCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                 <  2:5893679/150‑1 (MQ=32)
                                      aCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                 <  1:4562072/150‑1 (MQ=32)
                                      aCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                 >  2:3874070/1‑150 (MQ=32)
                                       cGCCCGCGCCGGTTGCGTGATGCGACGCTGGCGCGTCTTCTCAGGCCTACACGGCTGTGAAGTGCTCCACCCCGTAGGTCGGATACGGCGGTTCCGCCGCATCCGACATCGACCGCCCTAGCCGGGTGCCTGATGCGACGGTGGCGCGTc                                                                                                                <  2:5414138/150‑1 (MQ=11)
                                       cGCCCGAGCCGGTGGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTGCACCCCGTAGGTCTGATTAGGCGGTTACGCCGCATCCGACATCTAACGCCCCAGTCGGTTTCCTGATGCGACGCTGGCGCGTc                                                                                                                <  1:5805845/150‑1 (MQ=11)
                                        gCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCt                                                                                                               <  2:2729991/150‑1 (MQ=31)
                                        gCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCt                                                                                                               <  1:4345965/150‑1 (MQ=31)
                                         cccGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCtt                                                                                                              <  1:3302160/150‑1 (MQ=11)
                                         cccGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCGAACGCCCAAGCCGGTTGCCTGTTGCGACGCTGGCGCGTCtt                                                                                                              >  2:1582403/1‑150 (MQ=11)
                                                                                                           aCCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCg                                            <  1:1689904/150‑1 (MQ=11)
                                                                                                            ccccGTAGGTCAGAAAAGGCGGTCACCCCTCATCCTACATCTAAAGAACAATCCGTTTGCATCATGCGACGTTGGGGAGTCTTATCAGGCCTACACAGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGa                                           <  2:4095625/150‑1 (MQ=11)
                                                                                                                gTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTTTCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATc                                       >  2:2694343/1‑150 (MQ=17)
                                                                                                                gTAGGTCGGATAAGGCGGTGACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATc                                       <  2:4614487/150‑1 (MQ=17)
                                                                                                                 tAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATcc                                      <  2:2137419/150‑1 (MQ=32)
                                                                                                                  aGGTCCGACAAGGCGGTCACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCa                                     <  1:5358888/150‑1 (MQ=16)
                                                                                                                   ggTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCaa                                    <  2:3218045/150‑1 (MQ=21)
                                                                                                                   ggTCGGATAAGGCGGTCACGCCGCATCCGACATCCAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCaa                                    <  2:3429954/150‑1 (MQ=21)
                                                                                                                    gTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGACGTGCTCCACCCCGTATGTTGGATAAGGCGGTTACGCCGCATCCGACATCGAAc                                   >  1:1662044/1‑150 (MQ=12)
                                                                                                                    gTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAAc                                   >  1:5979800/1‑150 (MQ=34)
                                                                                                                     tCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACg                                  >  1:1247857/1‑150 (MQ=34)
                                                                                                                         aTAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCg                              <  1:3610722/150‑1 (MQ=37)
                                                                                                                         aTAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCg                              <  1:9950/150‑1 (MQ=37)
                                                                                                                          tAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGGTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGa                             <  1:4879372/150‑1 (MQ=37)
                                                                                                                          caaGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGa                             <  1:5018759/149‑1 (MQ=2)
                                                                                                                            aGGCGGTGACGCCGCATCCGACATCTAACGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGc                           <  2:2971444/150‑1 (MQ=255)
                                                                                                                             ggCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGcc                          >  2:5570851/1‑150 (MQ=37)
                                                                                                                              gCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCg                         <  1:629555/150‑1 (MQ=37)
                                                                                                                               cggaaaCGCCGCATCAGACATCAAACGCCCAAGCCTGTTGCCTGATGCGACGCTGTCGCGTCTTAACAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTCGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCgg                        <  2:2568523/145‑1 (MQ=11)
                                                                                                                                ggTCACGCCGCATCCGACATCCAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGt                       >  2:4324208/1‑150 (MQ=255)
                                                                                                                                 gTTACGCCGCATCCGACATCTAACGCCCCCGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGtt                      <  1:2159379/150‑1 (MQ=255)
                                                                                                                                 gTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGtt                      <  1:2159260/150‑1 (MQ=37)
                                                                                                                                 gTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGtt                      >  1:5275127/1‑150 (MQ=21)
                                                                                                                                  ttACGCCGCATCCGACATCTACCGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTg                     <  2:4735918/150‑1 (MQ=255)
                                                                                                                                  ttACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGAGGCGACGCTGGCGCGTCGTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTAGGATCAGGCGGTTACGCCGCAGCCGACATCCACCGCCCGAGCCGGTTg                     >  2:1103434/1‑150 (MQ=11)
                                                                                                                                   tACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGc                    <  1:4994708/150‑1 (MQ=37)
                                                                                                                                    aCGCCGCATCCGACATCTATCGCCCAAGCCGGGTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGcc                   <  1:3810712/150‑1 (MQ=255)
                                                                                                                                    aCGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCGGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGcc                   <  1:1292482/150‑1 (MQ=37)
                                                                                                                                    aCGCCGAATCCGTAATCTCACGCGCTAGCGGTTTGCCTTATGCGACTCTTGCGCGTCTTATGAGGCCTACACCGCTGTGAAGTGCGCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGcc                   <  1:4144045/150‑1 (MQ=11)
                                                                                                                                       ccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACAt                                        <  2:3912363/126‑1 (MQ=17)
                                                                                                                                       ccgcATCCGACATCCACCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGa                <  1:2170162/150‑1 (MQ=255)
                                                                                                                                         gcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGGTGGATCAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATg              >  2:710051/1‑150 (MQ=255)
                                                                                                                                              cGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCAGATGCGACg         >  1:1133092/1‑150 (MQ=37)
                                                                                                                                               gACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGc        >  2:1737394/1‑150 (MQ=37)
                                                                                                                                                  aTCTAACGCCCACGCCGGTTGCCTGATGCGACGCGGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTgg     <  1:1582403/150‑1 (MQ=255)
                                                                                                                                                   tCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgc    <  2:2285168/150‑1 (MQ=37)
                                                                                                                                                    cTACCGCCCCAGCCGGTGGCCTGATGCGACGCTGGCGCGTCTTATCTGGGCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTCACGCCGCATCCGACATCCAACGGCCGAGCCGGTTGCCTGATGCGACGCTGgcg   <  2:5632536/150‑1 (MQ=11)
                                                                                                                                                     tAACGCCCAAGCCGTTTGCCTGATGCGACTCTGGAGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcgc  <  1:2157005/150‑1 (MQ=255)
                                                                                                                                                     tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcgc  >  2:5899019/1‑150 (MQ=37)
                                                                                                                                                     tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcgc  <  1:5151017/150‑1 (MQ=37)
                                                                                                                                                     tAACGCCCAAGCCGGTTGCCAGATGCGACGCGGGCGCGCCATAGAAGGCCAACACCCCAGTGAAGTGCTACACCCCCAAGGTTGGATAAGGCGGTTACGCCGCATCCGACAACCAACGGCCCACCCCGTAGCCTGATGCGACGCTGgccc  >  1:5660398/1‑148 (MQ=11)
                                                                                                                                                     |                                                                                                                                                     
TAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGC  >  NC_000913/2304531‑2304829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: