Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,725,824 C→A 6.0% intergenic (‑78/+245) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,725,8240CA 6.0% 1133.0 / 7.3 337intergenic (‑78/+245)kgtP/rrfGalpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
Reads supporting (aligned to +/- strand):  ref base C (97/204);  new base A (33/2);  total (130/207)
Fisher's exact test for biased strand distribution p-value = 4.12e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 340 total aligned reads displayed.

AATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCA  >  NC_000913/2725675‑2725972
                                                                                                                                                     |                                                                                                                                                    
aaTCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATAc                                                                                                                                                      >  2:804306/1‑150 (MQ=255)
   cGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTGTAAATGAAATATTATAAAtt                                                                                                                                                   >  2:1856825/1‑150 (MQ=255)
    gCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACAttt                                                                                                                                                  <  2:2655283/150‑1 (MQ=255)
    gCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACAttt                                                                                                                                                  <  1:1665791/150‑1 (MQ=255)
      ccAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTGTTTATGTAATATTATAAATttgt                                                                                                                                                >  1:2079463/1‑150 (MQ=255)
      ccAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGGCGCTGTTGTTTTTTTTTTTTTTAAAGGGAATATTATACATttgt                                                                                                                                                >  1:5065833/1‑150 (MQ=255)
          aTGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAATGAAATATTATAAATTTTTTTCa                                                                                                                                            >  1:3135723/1‑150 (MQ=255)
          aTGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCa                                                                                                                                            <  2:22369/150‑1 (MQ=255)
          aTGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCa                                                                                                                                            <  2:3445417/150‑1 (MQ=255)
           tGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCat                                                                                                                                           >  1:2755297/1‑150 (MQ=255)
                 gacgaGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCAtt                                                                                                                                     <  2:1258656/150‑1 (MQ=255)
                  acgaGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTa                                                                                                                                    <  1:5166982/150‑1 (MQ=255)
                      gTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTTTAAAGGAAATATTATAAATTTTTTTCATATAATTATTCa                                                                                                                                >  1:302995/1‑150 (MQ=255)
                       tATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTTTAAAGGAAATATTATAAATTTTTTTCATATAATTATTCaa                                                                                                                               >  2:5634323/1‑150 (MQ=255)
                       tATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCaa                                                                                                                               <  2:2400505/150‑1 (MQ=255)
                           actactTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCtt                                                                                                                           >  1:3804363/1‑150 (MQ=255)
                               ctTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATAAATTTGTTGGATATAAATATGCAAccaaaaac                                                                                                                       >  2:3072669/1‑144 (MQ=255)
                                 tGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCAt                                                                                                                     <  2:5917560/150‑1 (MQ=255)
                                  gTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGTAATATTATACCTTTGTTGCCTATCATTATGGAACCTTAACCATg                                                                                                                    >  2:4794173/1‑150 (MQ=255)
                                   tCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATTCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGa                                                                                                                   <  2:659866/150‑1 (MQ=255)
                                   tCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTTTAAAGGAAATATTATACAATTTTTTCATAAAAATATGCAACCATAAACaata                                                                                                                   >  2:5029396/1‑147 (MQ=255)
                                       tttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGAAACCTTAACCATGAAttt                                                                                                               >  1:3879914/1‑150 (MQ=255)
                                       tttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATAAATTTGTTGCATATAATTATGCAACCATAACCATGAAttt                                                                                                               >  1:3318185/1‑150 (MQ=255)
                                        ttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATtta                                                                                                              <  1:538556/150‑1 (MQ=255)
                                           cTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTTTAAAGGTAAAATTATAAATTTTTTTCATATCATTATTCAAACATAACCAAGAATttagtt                                                                                                           >  1:340330/1‑150 (MQ=255)
                                            tGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCATAACCAAGAATttagtta                                                                                                          >  2:1918942/1‑150 (MQ=255)
                                             gTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATttagttag                                                                                                         <  2:2960878/150‑1 (MQ=255)
                                               cTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCa                                                                                                       <  1:4003445/150‑1 (MQ=255)
                                               cTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTCTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCa                                                                                                       <  1:1513239/150‑1 (MQ=255)
                                                 gCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGGTTTTTTATTTTTTTTTTTAAGGGAAAATTATAAATTTTTTGGAAATCATTATGCAAACATAAACAAGAATTTAGTTTGAAgg                                                                                                     >  1:5741957/1‑150 (MQ=255)
                                                  ccGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCATAACCAAGAAATTAGTTAGCAGGa                                                                                                    >  2:5338548/1‑150 (MQ=255)
                                                   cGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTTTTTTTTTTTTTTTTAAATGGAATATTATAAATTTGTTGGATAACATTATTCAACCAAAAACAAGTATTTATTTTGCAGGaa                                                                                                   >  1:1096839/1‑150 (MQ=255)
                                                      tACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAAt                                                                                                <  1:3246067/150‑1 (MQ=255)
                                                      tACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGGTTGTTTATTTTTTTTTTTAAGGCAAAATTAAACAATTGTTGCATATCATTATGCAAACATAACAAAGAATTTAGTTAGCAGGAAAAt                                                                                                >  1:4899587/1‑150 (MQ=255)
                                                       aCAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATg                                                                                               <  1:1366373/150‑1 (MQ=255)
                                                       aCAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATg                                                                                               <  2:5428198/150‑1 (MQ=255)
                                                       aCAGTACTTTCAGCCATGACATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATg                                                                                               <  1:4231679/150‑1 (MQ=255)
                                                          gTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGGTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTTAATGAAATATTATAATTTTTTTTCATATGATTTTTCTAACTTAAACATGTATTTTGTTTGCAGTAAAATGGtt                                                                                            >  2:5734690/1‑150 (MQ=255)
                                                          gTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTTTTTATTTTTTTTGTAAATGAAATATTATAAATTTTTTGAATATAATTATTCAACCATAAACATGAATTTATTTATCAGGAAAATTGtt                                                                                            >  1:348457/1‑150 (MQ=255)
                                                           tACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTa                                                                                           <  2:2086609/150‑1 (MQ=255)
                                                              tttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATTCAACCATAACCAAGAATTTAGTTAGCAGGAAAATGGTTATTg                                                                                        >  2:1752644/1‑150 (MQ=255)
                                                              tttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTg                                                                                        >  2:1001189/1‑150 (MQ=255)
                                                              tttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTg                                                                                        >  2:4695903/1‑150 (MQ=255)
                                                              tttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTGTTGTTTATTTTTTTTTTTAAGGAAAAATTAAACAATTTTTTCATATAAATATGCAAACATAAACAAGAAATTATTTAGCAGGAAAATGGTTATTg                                                                                        >  1:2494555/1‑150 (MQ=255)
                                                              tttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTTTTTTTTTTGTAAAGGTAATATTAAAAATTTTTTTCATAAAAATATTCAACCATAAACAAGAATTTATTTATCAGGTAAAATGTTTTTg                                                                                        >  1:2199177/1‑150 (MQ=255)
                                                              tttCAGCCATGCCATTATGTCTCCTGCCGTAATACGATGCTTTTGACGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTTTTGCATATCATTATGCAACCTTAAACATGAATTTAGTTAGCATGTAAATGGTTATTg                                                                                        >  2:3342988/1‑150 (MQ=255)
                                                               ttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTga                                                                                       <  2:3667771/150‑1 (MQ=255)
                                                                 cAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTgagg                                                                                     <  2:3947923/150‑1 (MQ=255)
                                                                    ccatgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGc                                                                                  <  2:4155308/150‑1 (MQ=255)
                                                                    ccatgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGc                                                                                  <  1:5427003/150‑1 (MQ=255)
                                                                        gccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTTTTTATTTTTTTTTTTAATGAAATAATATAAAATTTTTTCATATAATTATTAAAACATAAACAAGAATTTATTTAGCAGGAAAATTGGTTTTGTGGTGCttta                                                                              >  1:917588/1‑148 (MQ=255)
                                                                         ccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAg                                                                             <  1:3279241/150‑1 (MQ=255)
                                                                         ccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAg                                                                             <  2:1605025/150‑1 (MQ=255)
                                                                          catTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAgg                                                                            <  1:1257214/150‑1 (MQ=255)
                                                                          catTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAgg                                                                            <  1:686880/150‑1 (MQ=255)
                                                                            gtATGTCTCCTGCCGTACTCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCAGGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGAt                                                                          <  2:3469346/149‑1 (MQ=255)
                                                                             tATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATa                                                                         <  1:4762866/150‑1 (MQ=255)
                                                                                 tctcCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTTCATATAATTATGCAACCTTAAACATGAATTTAGTTAGCAGGAAAATTGTTATTGAGGAGCTTAAGGATAAAtt                                                                     >  2:5655076/1‑150 (MQ=255)
                                                                                    ccTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCt                                                                  <  1:5685338/150‑1 (MQ=255)
                                                                                    ccTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCt                                                                  <  1:2381001/150‑1 (MQ=255)
                                                                                     cTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTg                                                                 <  2:5249935/150‑1 (MQ=255)
                                                                                      tGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGTAAATAATATACATTTTTTGCATATCATTAAGCAACCATAACCATGAATTTAGTTAGCAGGAAAAAGGTTATTGAGGAGCTTAAGGATAAAATTcgtg                                                                >  2:2099490/1‑147 (MQ=255)
                                                                                      tGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTgg                                                                >  2:2002044/1‑150 (MQ=255)
                                                                                          gTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAag                                                            <  2:5063961/150‑1 (MQ=255)
                                                                                             agccGATGCTTTTGTCGGTCGCTTTTGTTTATTTTGTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAaggag                                                         <  2:5861902/148‑1 (MQ=255)
                                                                                             aTCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAaggag                                                         <  2:3212922/150‑1 (MQ=255)
                                                                                               ccGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAggagga                                                       <  2:2755407/150‑1 (MQ=255)
                                                                                                  aTGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGacac                                                    <  1:616344/150‑1 (MQ=255)
                                                                                                       tttGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATg                                               <  2:3256257/150‑1 (MQ=255)
                                                                                                           tcggtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAg                                           <  1:1181640/150‑1 (MQ=255)
                                                                                                            cggtctCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTTCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGt                                          <  2:3277608/150‑1 (MQ=255)
                                                                                                             ggtcgCTTTTTTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTg                                         <  2:4034613/150‑1 (MQ=255)
                                                                                                              gtcgCTTTTTTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTgg                                        <  2:1458520/150‑1 (MQ=255)
                                                                                                                 gCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTTCATATCATTATGCAACCTTAACCATGAATTTATTTAGCAGGAAAATGGTTATTGAGGAGCATAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCa                                     >  1:5556679/1‑150 (MQ=255)
                                                                                                                      tGTTTATTTTTTTTGTAAATGAAATATTATACATTTGTTGAATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTg                                <  1:1584600/150‑1 (MQ=255)
                                                                                                                      tGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTg                                <  1:5224663/150‑1 (MQ=255)
                                                                                                                      tGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTg                                <  1:4583912/150‑1 (MQ=255)
                                                                                                                       gTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTgg                               <  2:1138668/150‑1 (MQ=255)
                                                                                                                        tttATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTggg                              <  2:2501648/150‑1 (MQ=255)
                                                                                                                          tATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGa                            >  1:2612254/1‑150 (MQ=255)
                                                                                                                           tttttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGaa                           <  1:188627/149‑1 (MQ=255)
                                                                                                                           aTTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAATTGGTTATTGAGGAGCTTATGGATAAATTTGTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGaa                           >  2:4379050/1‑150 (MQ=255)
                                                                                                                           aTTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGaa                           <  1:3090925/150‑1 (MQ=255)
                                                                                                                           aTTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGaa                           <  1:3715510/150‑1 (MQ=255)
                                                                                                                            ttttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAg                          >  2:3814472/1‑150 (MQ=255)
                                                                                                                             tttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAATTGGTTTTTGTGGTGCTTTAGGATAATTTTGTGGTTAGGTGGACACGTTTGGTAGTGGGGAAGTTGGGGAAGc                         >  2:1031314/1‑150 (MQ=255)
                                                                                                                             tttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGc                         >  2:1076558/1‑150 (MQ=255)
                                                                                                                             tttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGc                         <  1:4342122/150‑1 (MQ=255)
                                                                                                                              ttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGcc                        >  2:386584/1‑150 (MQ=255)
                                                                                                                              ttttttGTAAAGGAAATATTATACATTTGTCGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGcc                        >  2:2828790/1‑150 (MQ=255)
                                                                                                                                       aaGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGtt               >  2:3169097/1‑150 (MQ=255)
                                                                                                                                        aGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTg              <  2:5081741/150‑1 (MQ=255)
                                                                                                                                        aGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTg              <  1:5029396/150‑1 (MQ=255)
                                                                                                                                         ggAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGc             >  1:2902163/1‑150 (MQ=255)
                                                                                                                                         ggAAATATTATACATTAGTTGCATATCATTATGCAACCTAAACCATGAATTTAGTTAGCAGGAAAATGGTTAGTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGc             >  2:550780/1‑150 (MQ=255)
                                                                                                                                               attatACATTTGTTGCATATCATTATGCAACCTTAACCCTGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATc       <  2:2078385/150‑1 (MQ=255)
                                                                                                                                                 tataCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTg     <  2:870409/150‑1 (MQ=255)
                                                                                                                                                  ataCATTTGTTGCATATCATTTTGCTACCTTAACCATGAATTTAGTTAGCAGGATAATGGTTTTTGAGGTGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGTAGTGGGCAAGTTGGGGAAGCCGTATACGTTGCTGAATCTgg    <  2:2615497/150‑1 (MQ=255)
                                                                                                                                                  ataCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTgg    <  1:4695903/150‑1 (MQ=255)
                                                                                                                                                  ataCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTgg    <  1:1001189/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCA  >  NC_000913/2725675‑2725972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: