Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,067 T→G 11.8% intergenic (+50/‑148) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,0670TG11.8% 740.5 / 20.0 263intergenic (+50/‑148)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (92/131);  new base G (35/3);  total (129/134)
Fisher's exact test for biased strand distribution p-value = 1.89e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 266 total aligned reads displayed.

AAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAT  >  NC_000913/3780919‑3781216
                                                                                                                                                    |                                                                                                                                                     
aaaTCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGtt                                                                                                                                                      <  2:6925410/150‑1 (MQ=255)
 aaTCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTc                                                                                                                                                     <  2:401703/150‑1 (MQ=255)
   tCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGGCCCCTGAACCGATAACTATAACTGAGGGTGAGGGGGCCGTTggg                                                                                                                                                   >  1:5840431/1‑150 (MQ=255)
    cGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgc                                                                                                                                                  <  1:405604/150‑1 (MQ=255)
     gATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcg                                                                                                                                                 <  2:82459/150‑1 (MQ=255)
     gATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATCGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCAAACCCAAACCCGCAGGGAGAGGGGGCCCTTCGgcg                                                                                                                                                 >  2:3006514/1‑150 (MQ=255)
       tCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCt                                                                                                                                               <  1:7190806/150‑1 (MQ=255)
        cAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtg                                                                                                                                              <  2:1372304/150‑1 (MQ=255)
        cAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtg                                                                                                                                              <  2:2299436/150‑1 (MQ=255)
         aGCGCAATTACGCAAGAGTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgt                                                                                                                                             <  2:4611017/150‑1 (MQ=255)
         aGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAACCCACAACCACAGGGAGGGGGGCCCGTTCGGCGgtgt                                                                                                                                             >  1:1045810/1‑150 (MQ=255)
         aGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAAACCGCTCCCACAGGGTGAGGGGACCCGTCGgcggggg                                                                                                                                             >  2:5916474/1‑146 (MQ=255)
          gctaaaTTATGCATGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCACCCTCACCCTAACCCTCTCCCTCTGGGAGTGGGGACCGTTCGGCGCtgta                                                                                                                                            <  1:5925132/147‑1 (MQ=255)
          gCGAACTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGACTGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgta                                                                                                                                            <  2:1722742/150‑1 (MQ=255)
          gCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCCAACCCACTCCCACAGGGAGAGGGGACCGGTAGGCGCagta                                                                                                                                            >  1:4734331/1‑150 (MQ=255)
           cGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCGCACCCCAACCCACGACCCCCTGGAGGGGGGGCCCGTCGgcgcgggtt                                                                                                                                           >  1:2704387/1‑145 (MQ=255)
            gAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCAAAAACACACCCACACGGGGAGGGGGCCCGTAGGGGCggtatg                                                                                                                                          >  2:1386604/1‑150 (MQ=255)
              aaTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAAACCACACCCACACGGAGAGGGGACCCGTCGGCGCggtattta                                                                                                                                        >  1:525419/1‑150 (MQ=255)
                 tACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTc                                                                                                                                     <  1:1330570/150‑1 (MQ=255)
                  aCGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTcc                                                                                                                                    <  1:1741581/150‑1 (MQ=255)
                    gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAAACCACTACCACACGGAGAGGGGACCCGTAGGCGCtgtatgtaaaacca                                                                                                                                  >  2:2863040/1‑144 (MQ=255)
                      aaGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCa                                                                                                                                <  2:7105834/150‑1 (MQ=255)
                      aaGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCa                                                                                                                                <  2:3586929/150‑1 (MQ=255)
                       aGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCGAAACCACTCCCCCCGGGCGGGGGGACCGGGCGGCGGGGGATGGACGCCCGCAc                                                                                                                               >  1:6791801/1‑150 (MQ=255)
                        gATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCAct                                                                                                                              <  2:4054454/150‑1 (MQ=255)
                         aTTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCACCCGAAACCGCTCCCCCAGGGAGAGGGGACCCGTCGGCGCGGTATGTCCACCCacacac                                                                                                                             >  2:3650678/1‑148 (MQ=255)
                              cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGTATGCGGCTTAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGTGTGGGGACCGTTCGGCGCTGTATGTACTCCCTCTCTCTGaaa                                                                                                                        <  1:5432274/150‑1 (MQ=255)
                              cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                                                                        <  1:2782879/150‑1 (MQ=255)
                              cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                                                                        <  1:179224/150‑1 (MQ=255)
                               tGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAAc                                                                                                                       <  2:215752/150‑1 (MQ=255)
                               tGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAAc                                                                                                                       <  2:1293407/150‑1 (MQ=255)
                               tGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAACCCACACCCACAGGGGGCGGGGGCCCTTCGGCGCGGTGTGTACACCCACACACTGAAAc                                                                                                                       >  1:6922714/1‑150 (MQ=255)
                                 gTGCAGGGGCTTGGTATAGAGTTGCCTGCGGAACTGGCTCCCATGGCGTATGGGAATGCGGCATAGTCGTTTGCGCCCCCTCAACATAACCCTCTCCCTCTGGGCTTGTGTACCGTTCGGCGCTGTTTGTTCTCCATCACTCTTAAACGa                                                                                                                     <  1:5500387/150‑1 (MQ=255)
                                   gctggggGTGGGTAATGTGTTGCCTGCGGCTCTGGCTCCCTTGGCTAATGGGAATGCGGCTTTGTCTTTTGCGCCCCCTCACCCTAACCCTCTCCCTATGGGTGTGGGGACCGTTCGGCGCTTTTTGTACTCCCTCACTCTGACACGaca                                                                                                                   <  1:1478041/147‑1 (MQ=255)
                                   gCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCGAACCCACACCCAACGGGCGAGGGGGCCCGTCGGCGCAGTATGTACGACCACACACAGaaaacccc                                                                                                                   >  1:4527831/1‑145 (MQ=255)
                                        ggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCTGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCa                                                                                                              <  2:5311849/150‑1 (MQ=255)
                                        ggCTGGGTAAAGAGTTGCCTGCGGCACTGGCGCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGGTCGGCGCTGGATGTACTCCCTCACTCTGAAACGACACCGCa                                                                                                              <  2:3511985/150‑1 (MQ=255)
                                         gCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCGAAACCACTCCCACAGGGAGAGGGGGCCGGTCGGCGCGGGGTGTACTCCCACCCACAGAAACCACACCCCAc                                                                                                             >  1:7074245/1‑150 (MQ=255)
                                               tAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAATGGGAATGCGTCATAGTCGTTTGCGCCCCCCCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCtttt                                                                                                       <  1:887850/150‑1 (MQ=255)
                                                aaaGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttt                                                                                                      <  2:5650647/150‑1 (MQ=255)
                                                aaaGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttt                                                                                                      <  2:6042899/150‑1 (MQ=255)
                                                  agagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCTTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttttt                                                                                                    <  2:6017345/150‑1 (MQ=255)
                                                      ttGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTcc                                                                                                <  1:451210/150‑1 (MQ=255)
                                                        gCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCt                                                                                              <  1:6424752/150‑1 (MQ=255)
                                                         ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCACACCCGAAACCAAAACCACAGGGAGAGGGGGCCCGTAGGGGCTGTGTGTACACCCGCACACAGAAACCACACCGCACTCATTTTTTATcccgc                                                                                             >  2:5200280/1‑148 (MQ=255)
                                                         ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCCCCCCAAAACACACCCCCCGGGGGCGGGGGCCCGGCGGCCCGGTTTTTTCTCCCGCACAAAGAAACCACACCCCCCACgttttttataacac                                                                                             >  2:3189001/1‑143 (MQ=255)
                                                            gCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGcc                                                                                          <  1:2877830/150‑1 (MQ=255)
                                                             cGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGccc                                                                                         <  2:6487893/150‑1 (MQ=255)
                                                             cGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGccc                                                                                         <  2:3465884/150‑1 (MQ=255)
                                                                 aCTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTcc                                                                                     <  1:6025279/150‑1 (MQ=255)
                                                                   tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgg                                                                                   <  2:5394440/150‑1 (MQ=255)
                                                                   tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCGAAACCTCAACCACACGGGGAGGGGGCCGGTGGGCGCTGTGTGGACTACCACACGATGAAACCACACCCCCCACTTTTTTTCTCCCGCGCCCCCCCgg                                                                                   >  2:6335714/1‑150 (MQ=255)
                                                                    ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTGCggg                                                                                  <  1:3860814/150‑1 (MQ=255)
                                                                     gCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCCCCCCAAACCACACCCACAGGGGGGGGGGGCCCGTGGGCGCGGGGTTTAATCCCCCACCCGGGAACCCCCCCCCCCGCATTTTTTGTACCACGCCCCGCCcggg                                                                                 >  2:3221084/1‑150 (MQ=255)
                                                                      cTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCCAAACCACAACCACAGGGCGAGGGGGCCCGTCGGCCCGGTGTGTTCTGCCGCAAACTGAAACCACCCCCCCCACATTTTTTAGACCGAGCCCCACCggggg                                                                                >  2:201670/1‑149 (MQ=255)
                                                                       tCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgag                                                                               <  1:269614/150‑1 (MQ=255)
                                                                          cATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACAACCCAAACCACACCCACACGGGGGGGGGTCCCTTGGGCGCGGTAATTTCTACCGCACTCAGTAAACACAACCCACGCTTTTTTTATACCACGCCCCCCCCGGGTGAgg                                                                            >  2:3208291/1‑150 (MQ=255)
                                                                            tGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggga                                                                          <  2:4126527/150‑2 (MQ=255)
                                                                              gCGAAAGCGAACGCGGCAAAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACCCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCg                                                                        <  2:3780644/150‑1 (MQ=255)
                                                                               cGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgg                                                                       <  2:5742159/150‑1 (MQ=255)
                                                                                gAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCggg                                                                      <  2:1751902/150‑1 (MQ=255)
                                                                                gAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCggg                                                                      <  1:3989684/150‑1 (MQ=255)
                                                                                 aaaGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgggg                                                                     <  1:7156615/150‑1 (MQ=255)
                                                                                 aaaGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCAAACCCACTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCACACTCTGAAACGACACCCCACTCTTATTTTATCCCTCGCCCCACCGGGGAGAGGGCCgggg                                                                     >  2:4753740/1‑150 (MQ=255)
                                                                                 aaaGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgggg                                                                     <  1:6675669/150‑1 (MQ=255)
                                                                                    gggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGTGAGGGGACCGTTCGGCGCTTTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGa                                                                  <  1:3203514/150‑1 (MQ=255)
                                                                                    gggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGa                                                                  <  1:5074035/150‑1 (MQ=255)
                                                                                     ggAATGCGGCATAGTCGTTTGCGCCCCCGCAACCAAAACCACTCCCTCAGGGGGAGGGGACCCGTCGGGGCGGTATGTAATACCTCACGCGGGAAAGACACCGCCCGCATTTTTTATGCCACGCCCCACCCGggcggggggcgggggggg                                                                 >  2:2818403/1‑146 (MQ=255)
                                                                                      gAATGCGGCATAGTCGTTTGCGCCCCCTCCCCCTAACCCGCTCCCTCAGGGAGAGGGGACCGGTCGGCGCTGTATGTCCCCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                <  1:3103469/150‑1 (MQ=255)
                                                                                           cGGCATAGTCGTTTGCGCCCCCTCACCCAAAACCACTCCCACAGGGAGAGGGGACCGTTCGGCGCGGTATGTACTCCCGCACGCTTAAACCACAACGCCCTCGTTTTTTATACCACCCCCCACCGGGGGGAGGGCCCGGGTGAgggggca                                                           >  2:5273394/1‑147 (MQ=255)
                                                                                             gCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGTAAAAg                                                         <  2:2172136/150‑1 (MQ=255)
                                                                                                       ttGCGCCCCCTTACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGc                                               >  2:323226/1‑150 (MQ=255)
                                                                                                             ccccTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGTGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCa                                         >  2:6996427/1‑150 (MQ=255)
                                                                                                             ccccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCa                                         >  2:2380644/1‑150 (MQ=255)
                                                                                                                cTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAtt                                      >  1:509904/1‑150 (MQ=255)
                                                                                                                cTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAtt                                      >  1:4945798/1‑150 (MQ=255)
                                                                                                                 taaCCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCGCTGCTCACCAAtgt                                     >  1:3956199/3‑148 (MQ=255)
                                                                                                                  cACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAtttt                                    >  1:5938234/1‑150 (MQ=255)
                                                                                                                      cTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGc                                >  1:1418574/1‑150 (MQ=255)
                                                                                                                      cTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAATTTCTGc                                >  2:2220503/1‑150 (MQ=255)
                                                                                                                       tAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCt                               >  1:2023667/1‑150 (MQ=255)
                                                                                                                        tccccTCTCCCTGTGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTa                              >  2:4229625/3‑150 (MQ=255)
                                                                                                                        tccccTCTCCCTCAGGGCGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCAGAAACGACACCGCACTCATTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGCAAAGGCCGCACTGCTCACCAATTTTCTGCTa                              >  2:4488939/3‑150 (MQ=255)
                                                                                                                        aaCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTa                              >  1:4832361/1‑150 (MQ=255)
                                                                                                                        aaCCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTa                              >  1:6443015/1‑150 (MQ=255)
                                                                                                                          cccTCTCCCTGAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAACAGGCCGCACTGCTCTCCAAGTTTCTGCTATc                            >  2:3866033/1‑150 (MQ=255)
                                                                                                                          cccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATc                            >  2:4265892/1‑150 (MQ=255)
                                                                                                                             tctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                         >  1:1061789/1‑150 (MQ=255)
                                                                                                                             tctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCCCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                         >  1:4193248/1‑150 (MQ=255)
                                                                                                                                 ccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcccc                     >  2:611677/1‑150 (MQ=255)
                                                                                                                                  cTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccccc                    >  1:3362267/1‑150 (MQ=255)
                                                                                                                                  cTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccccc                    >  2:3033226/1‑150 (MQ=255)
                                                                                                                                   tCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCg                   >  1:4646641/1‑150 (MQ=255)
                                                                                                                                   tCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCg                   >  1:6554963/1‑150 (MQ=255)
                                                                                                                                        gagaGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTa              >  1:4179482/1‑150 (MQ=255)
                                                                                                                                           agGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgg           >  1:4288945/1‑150 (MQ=255)
                                                                                                                                             gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg         >  2:2710469/1‑150 (MQ=255)
                                                                                                                                               gACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGc       >  2:2440460/1‑150 (MQ=255)
                                                                                                                                               gACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGc       >  1:987196/1‑150 (MQ=255)
                                                                                                                                                   gTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTa   >  1:2415298/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAT  >  NC_000913/3780919‑3781216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: