Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,959,457 T→G 9.5% intergenic (+12/+75) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,959,4570TG 9.5% 1044.1 / 24.2 386intergenic (+12/+75)ilvC/ppiCketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
Reads supporting (aligned to +/- strand):  ref base T (108/232);  new base G (43/3);  total (151/235)
Fisher's exact test for biased strand distribution p-value = 2.22e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 391 total aligned reads displayed.

GCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGG  >  NC_000913/3959308‑3959606
                                                                                                                                                     |                                                                                                                                                     
gCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAt                                                                                                                                                       >  1:6436809/1‑150 (MQ=255)
 cTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATg                                                                                                                                                      <  2:146737/150‑1 (MQ=255)
  tATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGc                                                                                                                                                     >  1:4489653/1‑150 (MQ=255)
   aTTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTTATGcc                                                                                                                                                    <  1:71905/150‑1 (MQ=255)
   aTTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGcc                                                                                                                                                    <  1:2372239/150‑1 (MQ=255)
    ttCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGccc                                                                                                                                                   >  2:3466756/1‑150 (MQ=255)
    ttCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGccc                                                                                                                                                   >  2:4992434/1‑150 (MQ=255)
    ttCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGccc                                                                                                                                                   >  1:4369926/1‑150 (MQ=255)
     tCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCt                                                                                                                                                  <  2:3677566/150‑1 (MQ=255)
         gAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCAcc                                                                                                                                              >  1:6287756/1‑150 (MQ=255)
              cgcgGTAGATAACGGGCAACTGCGTGATGTGAACGAAGTGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGAc                                                                                                                                         <  1:721417/150‑1 (MQ=255)
               gcgGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGAcc                                                                                                                                        <  1:5132152/150‑1 (MQ=255)
                cgGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGAccc                                                                                                                                       >  2:3589496/1‑150 (MQ=255)
                cgGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGAccc                                                                                                                                       >  1:2948593/1‑150 (MQ=255)
                 ggTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCct                                                                                                                                      <  2:1941815/150‑1 (MQ=255)
                 ggTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCACAACCCCAccca                                                                                                                                      >  1:2150244/1‑149 (MQ=255)
                   tAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCctct                                                                                                                                    <  1:997129/150‑1 (MQ=255)
                    aGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCtctc                                                                                                                                   <  1:2535448/150‑1 (MQ=255)
                    aGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCtctc                                                                                                                                   <  1:5368672/150‑1 (MQ=255)
                     gATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTcc                                                                                                                                  <  2:1094357/150‑1 (MQ=255)
                     gATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTcc                                                                                                                                  <  1:3627081/150‑1 (MQ=255)
                          cGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACag                                                                                                                             <  1:1983750/150‑1 (MQ=255)
                           gggCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCCACCCTCTccccccgg                                                                                                                            >  2:4007708/1‑145 (MQ=255)
                             gCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACAACCCCACCCACAACcacaccgc                                                                                                                          >  1:3860001/1‑146 (MQ=255)
                              cAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggag                                                                                                                         <  1:1363799/150‑1 (MQ=255)
                               aaCTGCGTGTTGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGATACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggaga                                                                                                                        <  2:6917952/150‑1 (MQ=255)
                               aaCTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGACACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACaggtaga                                                                                                                        <  2:4449382/150‑1 (MQ=255)
                               aaCTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggaga                                                                                                                        <  2:2438381/150‑1 (MQ=255)
                                aCTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCTGtgggagag                                                                                                                       <  2:1599854/150‑1 (MQ=255)
                                aCTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagag                                                                                                                       <  1:5846269/150‑1 (MQ=255)
                                 cTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCTGtgggagagg                                                                                                                      <  2:1290113/150‑1 (MQ=255)
                                   gCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCGACCCACTCCCACacggcgggggc                                                                                                                    >  2:5171909/1‑149 (MQ=255)
                                     gTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAgggagagggaga                                                                                                                  <  2:139031/150‑1 (MQ=255)
                                     gTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCACACCCCGCCCCACACCCACCgggagagggcga                                                                                                                  >  1:6365713/1‑150 (MQ=255)
                                      tGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGGGCTGACCCCGGCCCGGTGCCAGagggggggggggtt                                                                                                                 >  2:2792473/1‑146 (MQ=255)
                                         tgtgAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCACCCCGACCCACACCCCCagggagagggaggacac                                                                                                              >  1:6216531/1‑145 (MQ=255)
                                          gtgAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCCCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAaca                                                                                                             <  1:6128866/150‑1 (MQ=255)
                                             aaCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTc                                                                                                          <  1:6068923/150‑1 (MQ=255)
                                              aCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTCCCACAGGGAGAGGGAGAAAAACCTCa                                                                                                         >  2:4099512/1‑150 (MQ=255)
                                               cGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCaa                                                                                                        <  1:23236/150‑1 (MQ=255)
                                                gAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTCAGTGCGCGCTGAGGCCCACACCCCCCCCCCCTCCCCCCGGGAGCGGGAGAAAACCCACAAg                                                                                                       >  1:1179531/1‑150 (MQ=255)
                                                gAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCGCCCCAACGGCGAGGGCGAAAACACACAAg                                                                                                       >  1:3442310/1‑150 (MQ=255)
                                                  aGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGACACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGc                                                                                                     <  2:6538781/150‑1 (MQ=255)
                                                  aGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGc                                                                                                     <  2:1663237/150‑1 (MQ=255)
                                                   gCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTCCCACAGGGAGAGGGAGAAAACACTCAAGGcc                                                                                                    >  2:6430795/1‑150 (MQ=255)
                                                    cGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGAGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTACCCAAGGGAGAGGGAGAAAAAAATAAAGGCCt                                                                                                   >  2:1994718/1‑150 (MQ=38)
                                                    cGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGAGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTAACGTGCGCGCTGATGCCCTCCCCCCGACCCTCACCCACCGGGAGAGGGGGAAAACAAACAAGGCCt                                                                                                   >  1:1629338/1‑150 (MQ=38)
                                                        tCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTtctc                                                                                               <  2:298292/150‑1 (MQ=255)
                                                          gCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGGCCTCACCCCGACCCTCTACCACATGGAGTGGGAGAAAACACTCAAGGCCttgtgct                                                                                             >  1:1047826/1‑145 (MQ=255)
                                                            aGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCACTACCACAGGGAGAGGGAGAAAACACGCAAGGCCATCTCCTgg                                                                                           >  1:4595840/1‑150 (MQ=39)
                                                             gCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGga                                                                                          <  1:4762751/150‑1 (MQ=255)
                                                              ccATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGgag                                                                                         <  1:5575039/150‑1 (MQ=255)
                                                                aTGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGaa                                                                                       <  2:5595300/150‑1 (MQ=255)
                                                                aTGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACACCCAAACGGGGCGGGAGAAAACACACAACGCCTACACCAGGCGaa                                                                                       >  1:175193/1‑150 (MQ=255)
                                                                 tGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCGCCCCACACCCCCAGGGAGAGGGGGACAACACACAATGCCATCTACGGgagacg                                                                                      >  1:3967322/1‑148 (MQ=255)
                                                                 tGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCACCCCCCCCCGCACCCACACCGGGGGGGAGAAAAAACACAAGGCCATCTCCCGGAGCAg                                                                                      >  2:2689980/1‑150 (MQ=255)
                                                                    gATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCAAACCCACCGGGGGAGGGCGAAAAAACACAAAGCCGTCTCCGGGAGAAGGcc                                                                                   >  1:1453255/1‑150 (MQ=255)
                                                                        gAGCAGGTAGGTAAGAAACGGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGc                                                                               <  2:2109144/150‑1 (MQ=255)
                                                                         aGCAGGTAGGTAAGAAACTGCGCGGCTTTATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGTGAAGGCCTTGCt                                                                              <  1:4291880/150‑1 (MQ=255)
                                                                         aGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCt                                                                              <  1:1100210/150‑1 (MQ=255)
                                                                         aGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCt                                                                              <  2:1794348/150‑1 (MQ=255)
                                                                              gtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTCCCACAGGGAGAGGGAGAAAAAACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTat                                                                         >  2:5289932/1‑149 (MQ=255)
                                                                              gtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCCACCCACTCCCACAGGGCGTGGGAGAAAACACAAAAGGCCATCACCGGGGGTAGGCCGTGGTATTAg                                                                         >  2:6267555/1‑150 (MQ=37)
                                                                              gtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCCACCCACAACCACAGGGGGCTGGAGAAAACACTCAATGCCATAACCAGGGGAATGCATTGCGATTAg                                                                         >  2:225340/1‑150 (MQ=255)
                                                                                 ggtaAGAACCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTg                                                                      <  1:6728842/150‑1 (MQ=255)
                                                                                   taAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCACACCCCCACCCACACCCACCCGGGGAGGGGGAAAAAACACAAGGCCTTCACCAGGAGACGGCCGTGCGAGTATTTGCg                                                                    >  1:579213/1‑150 (MQ=255)
                                                                                    aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCgg                                                                   <  1:4997527/150‑1 (MQ=255)
                                                                                    aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCgg                                                                   <  1:5255883/150‑1 (MQ=255)
                                                                                    aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCCCCCACGCCCACCGGGGGGGGGAGAAAAAACGCAAGGCCATCGCCGTGAGAAGGGCGTGCGAGTAGTTGccg                                                                   >  2:5051607/1‑148 (MQ=255)
                                                                                         aCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAg                                                              <  1:3557777/150‑1 (MQ=255)
                                                                                          cTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGc                                                             <  1:4775991/150‑1 (MQ=255)
                                                                                           tGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCCCCCCACACCCCCCCGGAGCGGGAGAAAACACACAAGGCCCTCACCGCGCGAAGGCCGGGCGATGACTTGCgggccaccc                                                            >  1:6637700/1‑143 (MQ=255)
                                                                                              gcgGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTGAGGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCCCCGGGAGAGGGAGAAAACAATCAAGGCCTTCTCCGGGCGAAGGCCTTGCGATTAGTTGCGGTCCAGCACCt                                                         >  2:4696369/1‑150 (MQ=37)
                                                                                                ggCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCAAACCCCCACCCACTCCCACACGGCGGGGGAGAAAAAACACAAGGCCGTCTCCAGGGGGAGGCCGTGCGATTAGTTGCGGTACACCACCGTa                                                       >  2:6139479/1‑150 (MQ=255)
                                                                                                  cTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat                                                     <  2:2178008/150‑1 (MQ=255)
                                                                                                      atGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTCCCCCAGGGAGAGGGCGGAAACACTCAAGGCCTTCTCCTGGCGAAGGCCGGGCTATTAGTTGCGGTGCAGCACCTTAatgttt                                                 >  1:5670869/1‑147 (MQ=38)
                                                                                                       tGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGGGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgt                                                <  1:705518/150‑1 (MQ=255)
                                                                                                       tGACAGATATGAAACGTATTGCTGGTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgt                                                <  1:1940603/150‑1 (MQ=255)
                                                                                                         aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTGCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa                                              <  1:4070919/150‑1 (MQ=255)
                                                                                                           aGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGata                                            <  1:2775344/150‑1 (MQ=255)
                                                                                                             atatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATc                                          <  2:3122162/150‑1 (MQ=255)
                                                                                                                  aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCCACCCTCTCCCACAGGGGGCGGGGGAAAAAACACAAGGCCTTCTCCTGGAGAAGGCCATGCTATTAGTTGCGGGACAGCACCTTAATGATGTGATATACGAAc                                     >  2:3318669/1‑150 (MQ=38)
                                                                                                                  aaaCGTATTGCAGTAGCGGGTTAAGTGCGCGCTGATTCCCACACCCCCACCCAAAACCACAGGGAGAGGGAGAAAACAAACAAGGACATCACCTGGGGAATGCCGTGCAAATAGTTGCCGTACAGCACCATAATTCAGTGATATCAGAAc                                     >  2:4339668/1‑150 (MQ=255)
                                                                                                                     cGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                  <  1:2625651/150‑1 (MQ=255)
                                                                                                                          tGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACCGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGGTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGtgtg                             <  1:6930099/150‑1 (MQ=255)
                                                                                                                               ttGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAATGCCTTCTCCTGGAGAAGGCCTTGCGATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCg                        >  2:7094349/1‑150 (MQ=255)
                                                                                                                               ttGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCg                        <  2:6029125/150‑1 (MQ=255)
                                                                                                                               ttGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTCCCACAGGGAGCGGGAGAAAACACTCAAGGCCATCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCATAATGATGTGATATCCGAACTGGGTGTGCAGCg                        >  2:3046461/1‑150 (MQ=255)
                                                                                                                                   gggTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGcc                    <  1:335737/150‑1 (MQ=255)
                                                                                                                                   gggTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGcc                    <  2:5405751/150‑1 (MQ=255)
                                                                                                                                       tAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACCGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTc                <  2:4859202/150‑1 (MQ=255)
                                                                                                                                         aGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTTTGCAGCGGGCCGGTCgg              <  2:5712789/150‑1 (MQ=255)
                                                                                                                                         aGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCgg              <  2:5463565/150‑1 (MQ=255)
                                                                                                                                            gcgcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCTCCTTAATGATGTGATATCCGACCTGGGTGTGCAGCGGGCCGGTCGGCTc           <  2:7216475/150‑1 (MQ=255)
                                                                                                                                            gcgcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTc           <  2:6785665/150‑1 (MQ=255)
                                                                                                                                              gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa         <  1:3220965/150‑1 (MQ=255)
                                                                                                                                              gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa         <  2:3843549/150‑1 (MQ=255)
                                                                                                                                                   gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc    <  1:3073885/150‑1 (MQ=255)
                                                                                                                                                   gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc    <  1:4130850/150‑1 (MQ=255)
                                                                                                                                                    aTGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCg   >  2:4180012/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGG  >  NC_000913/3959308‑3959606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: