Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,967,449 C→T 43.9% L345F (CTC→TTC)  rho → transcription termination factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,967,4490CT43.9% 184.4 / inf 386L345F (CTC→TTC) rhotranscription termination factor
Reads supporting (aligned to +/- strand):  ref base C (94/121);  new base T (76/92);  total (171/215)
Fisher's exact test for biased strand distribution p-value = 8.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 402 total aligned reads displayed.

TCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGAT  >  NC_000913/3967301‑3967598
                                                                                                                                                    |                                                                                                                                                     
tCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACct                                                                                                                                                      <  2:4307249/150‑1 (MQ=255)
  gTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACctct                                                                                                                                                    <  1:1950876/150‑1 (MQ=255)
         aCGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTaa                                                                                                                                             <  1:6725633/150‑1 (MQ=255)
         aCGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTaa                                                                                                                                             >  1:692412/1‑150 (MQ=255)
            cttcttTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGAt                                                                                                                                          <  2:6789241/150‑1 (MQ=255)
            cttcttTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGAt                                                                                                                                          <  2:6420817/150‑1 (MQ=255)
             ttcttTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGACTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAACTGGACGAATTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATc                                                                                                                                         <  1:8741/150‑1 (MQ=255)
                tttGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCt                                                                                                                                      <  2:3782090/150‑1 (MQ=255)
                tttGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCt                                                                                                                                      <  1:354681/150‑1 (MQ=255)
                tttGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCt                                                                                                                                      >  1:2897132/1‑150 (MQ=255)
                 ttGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTg                                                                                                                                     >  2:3525768/1‑150 (MQ=255)
                  tGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGa                                                                                                                                    <  2:5428552/150‑1 (MQ=255)
                    gTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGaaa                                                                                                                                  >  1:1052996/1‑150 (MQ=255)
                        ggcgCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAAcg                                                                                                                              <  2:2979803/150‑1 (MQ=255)
                         gcgcGTACCGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATAGCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAAcgc                                                                                                                             <  1:702558/150‑1 (MQ=255)
                         gcgcGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAAcgc                                                                                                                             <  2:5507053/150‑1 (MQ=255)
                         gcgcGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAAcgc                                                                                                                             <  2:3990335/150‑1 (MQ=255)
                         gcgcGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAAcgc                                                                                                                             <  2:4859517/150‑1 (MQ=255)
                         gcgcGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAAcgc                                                                                                                             <  2:1902582/150‑1 (MQ=255)
                         gcgcGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAAcgc                                                                                                                             <  2:5982556/150‑1 (MQ=255)
                             gTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCt                                                                                                                         <  1:3519748/150‑1 (MQ=255)
                                aCGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTcc                                                                                                                      <  2:2312626/150‑1 (MQ=255)
                                 cGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACGGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTccc                                                                                                                     <  2:4983110/150‑1 (MQ=255)
                                  gTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCg                                                                                                                    <  1:5420483/150‑1 (MQ=255)
                                   tGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCgg                                                                                                                   <  2:882280/150‑1 (MQ=255)
                                         agGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCg                                                                                                             >  1:2177535/1‑150 (MQ=255)
                                          gggCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACGTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGa                                                                                                            <  1:1678334/150‑1 (MQ=255)
                                          gggCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGa                                                                                                            <  2:1113871/150‑1 (MQ=255)
                                           ggcggcAGCCTGACCATTCTCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGAc                                                                                                           >  2:530898/1‑150 (MQ=255)
                                           ggcggcAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGAc                                                                                                           <  1:5035677/150‑1 (MQ=255)
                                           ggcggcAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGAc                                                                                                           <  1:468097/150‑1 (MQ=255)
                                            gcggcAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACt                                                                                                          >  2:3405112/1‑150 (MQ=255)
                                             cggcAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTa                                                                                                         >  1:603730/1‑150 (MQ=255)
                                                cAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTATAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACaa                                                                                                      <  1:6638485/150‑1 (MQ=255)
                                                cAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACaa                                                                                                      <  1:398492/150‑1 (MQ=255)
                                                cAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACaa                                                                                                      <  2:6548444/150‑1 (MQ=255)
                                                 aGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAAc                                                                                                     >  2:1663664/1‑150 (MQ=255)
                                                 aGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAAc                                                                                                     <  1:574473/150‑1 (MQ=255)
                                                 aGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAAc                                                                                                     <  1:1757067/150‑1 (MQ=255)
                                                    cTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGt                                                                                                  >  1:1225004/1‑150 (MQ=255)
                                                    cTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGt                                                                                                  >  1:139807/1‑150 (MQ=255)
                                                    cTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGt                                                                                                  >  1:305494/1‑150 (MQ=255)
                                                    cTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGt                                                                                                  >  2:5756367/1‑150 (MQ=255)
                                                      gACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTc                                                                                                <  1:3461308/150‑1 (MQ=255)
                                                       aCCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCt                                                                                               <  1:6401197/150‑1 (MQ=255)
                                                         cATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTgg                                                                                             <  1:952650/150‑1 (MQ=255)
                                                            tATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTAc                                                                                          <  2:4546700/150‑1 (MQ=255)
                                                             aTCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTAcc                                                                                         <  2:1483357/150‑1 (MQ=255)
                                                              tCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTAccc                                                                                        >  1:3357934/1‑150 (MQ=255)
                                                              tCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTAccc                                                                                        <  1:754778/150‑1 (MQ=255)
                                                              tCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTAccc                                                                                        >  2:311269/1‑150 (MQ=255)
                                                              tCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAAAATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTAccc                                                                                        >  2:3334343/1‑150 (MQ=255)
                                                                gcgACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGt                                                                                      >  2:916265/1‑150 (MQ=255)
                                                                  gACGGCGCTTAGCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACAGGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTaa                                                                                    <  2:359165/150‑1 (MQ=255)
                                                                     ggCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAaaga                                                                                 <  2:5588469/150‑1 (MQ=255)
                                                                      gcgcTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAaagaa                                                                                <  2:1602287/150‑1 (MQ=255)
                                                                       cgcTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAaagaag                                                                               <  2:6736332/150‑1 (MQ=255)
                                                                       cgcTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTAGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAaagaag                                                                               <  1:1560878/150‑1 (MQ=255)
                                                                          ttATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAgc                                                                            >  1:2600854/1‑150 (MQ=255)
                                                                            aTCGAGACCGGTGCTAAACTGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAgctg                                                                          <  1:2037102/150‑1 (MQ=255)
                                                                                   ccGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGa                                                                   <  1:2114587/150‑1 (MQ=255)
                                                                                    cGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGAc                                                                  >  1:5962783/1‑150 (MQ=255)
                                                                                     ggTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACt                                                                 <  2:2560735/150‑1 (MQ=255)
                                                                                         cTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAgg                                                             <  2:2210462/150‑1 (MQ=255)
                                                                                         cTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAgg                                                             >  1:4566605/1‑150 (MQ=255)
                                                                                          tAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGga                                                            >  2:7157244/1‑150 (MQ=255)
                                                                                              aTGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGgaagaa                                                        <  2:2107731/150‑1 (MQ=255)
                                                                                              aTGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCACGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGgaagaa                                                        <  2:4917356/150‑1 (MQ=255)
                                                                                               tGGACGAAGTTATCTACGAAGAGTTTAATGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAAc                                                       <  1:1176522/150‑1 (MQ=255)
                                                                                                  aCGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGc                                                    >  1:3181684/1‑150 (MQ=255)
                                                                                                  aCGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGc                                                    >  2:123694/1‑150 (MQ=255)
                                                                                                    gAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAg                                                  >  2:1448298/1‑150 (MQ=255)
                                                                                                         tATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAAt                                             >  1:4233939/1‑150 (MQ=255)
                                                                                                           tCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAAtgt                                           <  2:2885822/150‑1 (MQ=255)
                                                                                                           tCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAAtgt                                           <  1:611889/150‑1 (MQ=255)
                                                                                                            cTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAAtgtg                                          >  1:3736147/1‑150 (MQ=255)
                                                                                                             tACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTgg                                         >  2:4713528/1‑150 (MQ=255)
                                                                                                             tACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTgg                                         <  1:2786514/150‑1 (MQ=255)
                                                                                                              aCGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGa                                        >  1:4347546/1‑150 (MQ=255)
                                                                                                                gAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATc                                      >  1:3622558/1‑150 (MQ=255)
                                                                                                                gAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATc                                      <  2:1807101/150‑1 (MQ=255)
                                                                                                                gAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATc                                      <  2:1723034/150‑1 (MQ=255)
                                                                                                                 aaGAGTTTAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATcc                                     <  1:2495634/150‑1 (MQ=255)
                                                                                                                 aaGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATcc                                     >  2:5151639/1‑150 (MQ=255)
                                                                                                                   gagTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTg                                   <  2:4457437/150‑1 (MQ=255)
                                                                                                                      tttAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTgcgc                                >  1:1313621/1‑150 (MQ=255)
                                                                                                                      tttAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTgcgc                                >  2:1328949/1‑150 (MQ=255)
                                                                                                                       ttAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCa                               <  1:6062664/150‑1 (MQ=255)
                                                                                                                        tAAAGGTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCaa                              >  2:3007581/1‑150 (MQ=255)
                                                                                                                         aatggTACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGAATACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCaaa                             <  2:2709153/147‑1 (MQ=255)
                                                                                                                             gTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCTGGAAGAACTGCAGAAAATGTGGATCCTGTGCAAAATCa                         <  1:5208464/150‑1 (MQ=255)
                                                                                                                             gTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCa                         >  2:408716/1‑150 (MQ=255)
                                                                                                                              tACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCAt                        >  2:5068575/1‑150 (MQ=255)
                                                                                                                              tACAGGCAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCAt                        <  1:1350606/150‑1 (MQ=255)
                                                                                                                                cAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTc                      >  1:4867101/1‑150 (MQ=255)
                                                                                                                                  ggCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCAc                    <  1:2296783/150‑1 (MQ=255)
                                                                                                                                   gCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCAcc                   >  2:6175156/1‑150 (MQ=255)
                                                                                                                                    cAACATGGAACTGCACTTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCAccc                  <  1:6518247/150‑1 (MQ=255)
                                                                                                                                          ggAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATggg            >  2:3610035/1‑150 (MQ=255)
                                                                                                                                                   ccTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGa   <  1:5588561/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCGTCCGAAACGCTTCTTTGGTGCGGCGCGTAACGTGGAAGAGGGCGGCAGCCTGACCATTATCGCGACGGCGCTTATCGATACCGGTTCTAAAATGGACGAAGTTATCTACGAAGAGTTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCTGCGCAAAATCATTCACCCGATGGGCGAAATCGAT  >  NC_000913/3967301‑3967598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: