Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,087,021 C→A 6.0% S7Y (TCT→TAT)  yiiG → DUF3829 family lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,087,0210CA 6.0% 1359.6 / 11.3 400S7Y (TCT→TAT) yiiGDUF3829 family lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (142/216);  new base A (38/2);  total (181/219)
Fisher's exact test for biased strand distribution p-value = 2.18e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 402 total aligned reads displayed.

TTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAG  >  NC_000913/4086874‑4087170
                                                                                                                                                   |                                                                                                                                                     
ttAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATTTTGAATCTATTATATATATATTTTTTATGCAATTAAATTTAAATGAATTTATTATAtt                                                                                                                                                     >  1:4175793/1‑150 (MQ=255)
ttAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTAATTTATTATTTTAAATCAATTATCTATATATTTATAAGTCAATTAAATGAAAAGGGATTTTTTATAtt                                                                                                                                                     >  1:598445/1‑150 (MQ=255)
 tAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTATCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAAATTATTATCTTc                                                                                                                                                    >  2:6288937/1‑150 (MQ=255)
  aaaTTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATTTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTcc                                                                                                                                                   >  2:3051391/1‑150 (MQ=255)
    aTTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGc                                                                                                                                                 >  2:4666969/1‑150 (MQ=255)
      tGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCta                                                                                                                                               <  1:6084168/150‑1 (MQ=255)
        cAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCtatt                                                                                                                                             <  2:7078369/150‑1 (MQ=255)
              ttATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTTGCGTTTTCTTTTTTCATTTATTATGTACACTCTCTTATCTAAATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTc                                                                                                                                       <  2:4011875/150‑1 (MQ=255)
                aTTCATTTCGTTAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGc                                                                                                                                     <  2:6934363/150‑1 (MQ=255)
                aTTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGc                                                                                                                                     <  1:1796955/150‑1 (MQ=255)
                aTTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGc                                                                                                                                     <  2:6168720/150‑1 (MQ=255)
                aTTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGc                                                                                                                                     >  2:4248675/1‑150 (MQ=255)
                aTTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTATTTTTTGATTTATTTTGTTGACACTCTTATCTATATATTTATTAAGCGATTAAATTAAAAAGAATTTTTTATATTACGCTATTATTGtgtc                                                                                                                                     >  1:4945629/1‑147 (MQ=255)
                 ttCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTGTTTTTTAATTTATTATTTTAACTCTCTTATCTATATATTTATAATGCAATTAAATTAAAAAGAATTTATTATATTAAGCGATTATATTAGcc                                                                                                                                    >  1:6499633/1‑150 (MQ=255)
                   cATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCcat                                                                                                                                  <  1:6534082/150‑1 (MQ=255)
                    aTTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTGTTTTTTGATTTATTATGTTCACACTCGTGTCGATATATTTATAATGCAATTAAAAGTAAAGGAATTTATTATATTACCCGAATATATTAGCcaac                                                                                                                                 >  2:1886998/1‑148 (MQ=255)
                       tCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATg                                                                                                                              <  1:1574089/150‑1 (MQ=255)
                       tCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATg                                                                                                                              <  2:5354911/150‑1 (MQ=255)
                       tCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATg                                                                                                                              <  1:1157170/150‑1 (MQ=255)
                        cGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGt                                                                                                                             >  2:2373020/1‑150 (MQ=255)
                        cGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGt                                                                                                                             <  1:5629646/150‑1 (MQ=255)
                        cGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTAATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATTAAAAGGAATTTATTATATTGAGCTATTATAGTAGCCATCATGt                                                                                                                             >  2:5622186/1‑150 (MQ=255)
                          tGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTcc                                                                                                                           <  1:1004395/150‑1 (MQ=255)
                           gAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTccc                                                                                                                          <  2:1181202/150‑1 (MQ=255)
                            aaTTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATTTTCACACTCTTATATATATATTTATAATGCAATTAAATGAAAAGGAATTTATTATGTTCCGCTATTATAGTCGCCAACATGTcccg                                                                                                                         >  2:3491478/1‑149 (MQ=255)
                             attttattattttattTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTc                                                                                                                        <  1:3835474/150‑1 (MQ=255)
                              ttttattattttattTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCg                                                                                                                       <  1:2599544/150‑1 (MQ=255)
                               tttattattttattTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCgg                                                                                                                      <  1:3190509/150‑1 (MQ=255)
                                ttattattttattTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGt                                                                                                                     <  1:3473694/150‑1 (MQ=255)
                                 tattattttattTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATTTTCACACTCGTATCTATATATTTATAAGGCCATTAAATTAAAAGGAATTTATTATCTTCCGCTATTATATTCGCCATCATGTACCACGGTc                                                                                                                    >  2:2173568/1‑150 (MQ=255)
                                              ataAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGtga                                                                                                       <  2:5318130/150‑1 (MQ=255)
                                              ataAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTAAGTTCACTCTAGTATCTATATATTTATAATGCAAATAAATGAAAAGGAAATTATTATATTACGCTATTATAGTACCCATCATGTACCTCGGTCTGACGGGTtgtgt                                                                                                       >  2:2134035/1‑149 (MQ=255)
                                               taAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTGATTTATTATGTTCAATCTCTTATATATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATATTACGCTATTATAGTGGCCATAATGTCCCTCGGTGTGTCGGGTTGtgat                                                                                                      >  2:4435684/1‑150 (MQ=255)
                                               taAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGtgat                                                                                                      <  2:173498/150‑1 (MQ=255)
                                                 aaCAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGtgatga                                                                                                    <  2:2619293/150‑1 (MQ=255)
                                                 aaCAAAATGCGTGTAGTGGCGTTTTCTTTTTTAAATTAATATGGTCCCTCACTTCTCTATATATTTATAATGCAATAAAATGAAAATGAATTTATTATATTCCCATATTATAGTCGCCAACAAGTCCCACGGGCTGACCGGGTGtggtga                                                                                                    >  2:1382130/1‑150 (MQ=255)
                                                  aCAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTgatgat                                                                                                   <  2:4096787/150‑1 (MQ=255)
                                                  aCAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTgatgat                                                                                                   <  2:6357990/150‑1 (MQ=255)
                                                       aTGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGGCGGGTTGTGATGATaaaaa                                                                                              >  2:3754389/1‑150 (MQ=255)
                                                        tGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATaaaaaa                                                                                             <  1:2915236/150‑1 (MQ=255)
                                                        tGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATaaaaaa                                                                                             <  2:6619727/150‑1 (MQ=255)
                                                          cGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGc                                                                                           <  2:2836190/150‑1 (MQ=255)
                                                          cGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGc                                                                                           <  1:5341827/150‑1 (MQ=255)
                                                             gtAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGa                                                                                        <  1:632850/150‑1 (MQ=255)
                                                             gtAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGa                                                                                        <  1:6879427/150‑1 (MQ=255)
                                                             gtAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGa                                                                                        <  2:5791689/150‑1 (MQ=255)
                                                             gtAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGa                                                                                        <  1:235262/150‑1 (MQ=255)
                                                                  ggCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAg                                                                                   <  2:5087495/150‑1 (MQ=255)
                                                                    cGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGaa                                                                                 <  2:4188575/150‑1 (MQ=255)
                                                                      ttttCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAAc                                                                               <  2:4572424/150‑1 (MQ=255)
                                                                         tCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCt                                                                            <  2:603715/150‑1 (MQ=255)
                                                                           ttttttCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTcc                                                                          >  2:1554365/1‑150 (MQ=255)
                                                                              tttCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGATTAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGc                                                                       <  2:2927223/150‑1 (MQ=255)
                                                                              tttCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGc                                                                       <  2:2073147/150‑1 (MQ=255)
                                                                              tttCAATTATTATGTTCACTCTCATATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGc                                                                       <  2:4793825/150‑1 (MQ=255)
                                                                                taaTTTTTTATGTACACTCTATTATCTATATATTTATAAGGAAATTAAATGAAATGTATTTTATTATCTTCCGCTTTTATAGTCGCCATCTTGTCCCTCGGTCTGAGGGGTTGTGTTTATAAAAAAGCCGAAACAGAAACGCTCCCGCCt                                                                     <  1:5126027/148‑1 (MQ=255)
                                                                                    ttattaTGTTCACTCTCTTATCTATATATTAATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCTTGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCa                                                                 <  1:7104784/150‑1 (MQ=255)
                                                                                       ttatGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATa                                                              >  2:3194603/1‑150 (MQ=255)
                                                                                       ttatGTTCACTCTCTTATATATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCTTGTCCCTGGGTCTGACGGGTTGTGTTGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATa                                                              <  1:2811569/150‑1 (MQ=255)
                                                                                         ttGTTCACTCTCTTATCTATATATTTATATGGCAATTAAATGAAATGTATTTTATTATCTTCCGCTATTATTGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATTATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGt                                                            <  1:4378423/149‑1 (MQ=255)
                                                                                            ttCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCaa                                                         <  1:4691852/150‑1 (MQ=255)
                                                                                              cACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAAcc                                                       >  1:5819674/1‑150 (MQ=255)
                                                                                                ctctctTATCTATATATTAATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTg                                                     >  1:4007020/1‑150 (MQ=255)
                                                                                                 tctctTATCTATATATTTATAAGGCAATTAAATGAAAAGTAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGc                                                    <  1:2192289/150‑1 (MQ=255)
                                                                                                   tctTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCg                                                  <  1:6258231/150‑1 (MQ=255)
                                                                                                    ctTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGc                                                 <  2:4366196/150‑1 (MQ=255)
                                                                                                         cTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAg                                            >  1:3364087/1‑150 (MQ=255)
                                                                                                          tatataTTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGc                                           <  2:3359955/150‑1 (MQ=255)
                                                                                                             atatTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTcc                                        <  2:5041323/150‑1 (MQ=255)
                                                                                                               atTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTg                                      >  2:5416979/1‑150 (MQ=255)
                                                                                                               atTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTg                                      >  2:2001109/1‑150 (MQ=255)
                                                                                                                 ttATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGaa                                    <  2:598445/150‑1 (MQ=255)
                                                                                                                  tataAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAg                                   >  1:1224203/1‑150 (MQ=255)
                                                                                                                   ataAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATTGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGc                                  <  1:6674524/150‑1 (MQ=255)
                                                                                                                   ataAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGc                                  <  1:6899904/150‑1 (MQ=255)
                                                                                                                       ggCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGaaa                              <  1:1976972/150‑1 (MQ=255)
                                                                                                                        gCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAAc                             >  1:7192632/1‑150 (MQ=255)
                                                                                                                        gCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAAc                             >  1:5695174/1‑150 (MQ=255)
                                                                                                                        gCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAAc                             >  2:6829923/1‑150 (MQ=255)
                                                                                                                         cAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAAcc                            <  1:3816317/150‑1 (MQ=255)
                                                                                                                          aaTTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCt                           >  1:3707768/1‑150 (MQ=255)
                                                                                                                            ttAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTAc                         >  2:5211791/1‑150 (MQ=255)
                                                                                                                              aaaTGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTg                       <  2:836024/150‑1 (MQ=255)
                                                                                                                               aaTGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGa                      >  1:5932409/1‑150 (MQ=255)
                                                                                                                               aaTGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGa                      <  1:449020/150‑1 (MQ=255)
                                                                                                                                 tGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAg                    >  2:75200/1‑150 (MQ=255)
                                                                                                                                 tGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAg                    >  1:6858169/1‑150 (MQ=255)
                                                                                                                                  gAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGc                   <  2:5927957/150‑1 (MQ=255)
                                                                                                                                     aaGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTcc                >  1:766805/1‑150 (MQ=255)
                                                                                                                                      aGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTccc               >  2:652640/1‑150 (MQ=255)
                                                                                                                                          aTTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGTCGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTg           <  2:5509794/150‑1 (MQ=255)
                                                                                                                                          aTTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTg           <  1:5643642/150‑1 (MQ=255)
                                                                                                                                           tttATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGc          <  2:1221026/150‑1 (MQ=255)
                                                                                                                                           tttATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGc          <  2:3832568/150‑1 (MQ=255)
                                                                                                                                              attatCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCaaa       >  1:5459025/1‑150 (MQ=255)
                                                                                                                                               ttatCTTCCGCTATTATAGTCGCCATGATTTCCCTCGGTCTGAGTGGTTGTGATGTTAAAAAATGCGAAACAGAAACGATACCGCCTGCCAATAGTCAAACTGCCGCACCAGCTCCTGAAGCGAAAACTACTGAAGCTCACTTTGAaaaa      >  2:2495856/1‑150 (MQ=255)
                                                                                                                                               ttatATTCCGATATTATAGTCGCCATCTTGTCCCTCGGTGTGACGGGTTTTGATTATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCaaaa      <  1:4599986/150‑1 (MQ=255)
                                                                                                                                                tatCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAg     <  1:7038330/150‑1 (MQ=255)
                                                                                                                                                 atCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGc    <  2:1100090/150‑1 (MQ=255)
                                                                                                                                                  tCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCa   <  1:3051391/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TTAAATTGCAGGACTTATTCATTTCGTGAATTTTATTATTTTATTTATAAACAAAATGCGTGTAGTGGCGTTTTCTTTTTTCATTTATTATGTTCACTCTCTTATCTATATATTTATAAGGCAATTAAATGAAAAGGAATTTATTATCTTCCGCTATTATAGTCGCCATCATGTCCCTCGGTCTGACGGGTTGTGATGATAAAAAAGCCGAAACAGAAACGCTCCCGCCTGCCAATAGTCAACCTGCCGCACCAGCTCCTGAAGCGAAACCTACTGAAGCTCCCGTTGCAAAAGCAG  >  NC_000913/4086874‑4087170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑