Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,153,447 A→G 5.0% intergenic (‑349/+249) ppc ← / ← argE phosphoenolpyruvate carboxylase/acetylornithine deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,153,4470AG 5.0% 1034.7 / 16.1 346intergenic (‑349/+249)ppc/argEphosphoenolpyruvate carboxylase/acetylornithine deacetylase
Reads supporting (aligned to +/- strand):  ref base A (125/185);  new base G (15/2);  total (147/199)
Fisher's exact test for biased strand distribution p-value = 1.54e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 352 total aligned reads displayed.

GCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAG  >  NC_000913/4153298‑4153596
                                                                                                                                                     |                                                                                                                                                     
gCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCTGCCTACa                                                                                                                                                       >  1:6553572/1‑150 (MQ=255)
    aaCTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTg                                                                                                                                                   <  1:4322438/150‑1 (MQ=255)
     aCTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTgg                                                                                                                                                  <  2:3718700/150‑1 (MQ=255)
      cTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTggg                                                                                                                                                 <  2:178271/150‑1 (MQ=255)
          tGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATc                                                                                                                                             <  2:5497471/150‑1 (MQ=255)
          gggAAATGTTAAAAAATCGCCCCACGGAACACCAAAGGTGGAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATc                                                                                                                                             <  2:1268786/149‑1 (MQ=255)
            gAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGt                                                                                                                                           >  1:4924741/1‑150 (MQ=255)
            gAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGAGGCACTGGAGTTTGGAAACAGTGCCGGATGAGGCGCGAGCGCCTTATCCGGCCTACAGTAGGGCATCGt                                                                                                                                           >  1:5793310/1‑150 (MQ=255)
             aaaTGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACTGTTGTGCATCggt                                                                                                                                          >  2:5348318/1‑148 (MQ=255)
             aaaTGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCggt                                                                                                                                          >  1:6251738/1‑148 (MQ=255)
             aaaTGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGtt                                                                                                                                          >  1:5598175/1‑150 (MQ=255)
              aaTGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGttt                                                                                                                                         <  1:6774777/150‑1 (MQ=255)
                tGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGa                                                                                                                                       <  2:2130558/150‑1 (MQ=255)
                tGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGa                                                                                                                                       <  1:6967759/150‑1 (MQ=255)
                tGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGa                                                                                                                                       <  1:5634035/150‑1 (MQ=255)
                   tAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGGGTGAGTc                                                                                                                                    >  2:2121124/1‑150 (MQ=255)
                    aaaaaaTCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCa                                                                                                                                   <  2:1735328/150‑1 (MQ=255)
                    aaaaaaTCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCa                                                                                                                                   <  1:5098081/150‑1 (MQ=255)
                     aaaaaTCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCCGGGTGTGAGTCAc                                                                                                                                  >  1:2180513/1‑150 (MQ=17)
                        aaTCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATAAGGCCTACAGTTGGGCATCTTTTGAGTCACTgt                                                                                                                               >  1:1027042/1‑150 (MQ=255)
                         aTCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtc                                                                                                                              >  1:194101/1‑150 (MQ=255)
                         aTCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtc                                                                                                                              >  1:1576492/1‑150 (MQ=255)
                          tCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtcg                                                                                                                             <  1:752971/150‑1 (MQ=255)
                           cGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtcgg                                                                                                                            <  1:5902678/150‑1 (MQ=255)
                            gCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtcggt                                                                                                                           <  1:6011194/150‑1 (MQ=255)
                            gCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtcggt                                                                                                                           <  2:672405/150‑1 (MQ=255)
                             ccccAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtcggtc                                                                                                                          >  1:38746/1‑150 (MQ=255)
                             ccccAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtcggtc                                                                                                                          <  1:4864085/150‑1 (MQ=255)
                             ccccAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCACCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtcggtc                                                                                                                          <  1:4014546/150‑1 (MQ=255)
                               ccAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGtcggtcgg                                                                                                                        >  2:2399264/1‑150 (MQ=255)
                               ccAAGTAACACCAAAGGTGTAGGTCGGAGAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGGTGGAGTCACTGtcggtcgg                                                                                                                        >  2:4696967/1‑150 (MQ=255)
                                cAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACGGGAGTTTGGCAACAGGGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTGGGGCGGCGTCTGAGTCACTGtcgggcggc                                                                                                                       >  2:936625/1‑149 (MQ=14)
                                 aaGTCACACCCAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGAt                                                                                                                      <  2:499591/150‑1 (MQ=255)
                                 aaGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGAt                                                                                                                      >  1:4684725/1‑150 (MQ=255)
                                  aGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATa                                                                                                                     >  2:3074885/1‑150 (MQ=255)
                                    tAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAg                                                                                                                   >  1:234946/1‑150 (MQ=255)
                                        acCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATgc                                                                                                               >  2:1357333/1‑150 (MQ=255)
                                          cAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGAGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTTGGCATCGTGTGAGGCTCTGACTGTCGGGAAAGATtcgc                                                                                                             >  2:775761/1‑150 (MQ=11)
                                          cAAAGGTGTAGGTCGGATAAGATGCGCAAGAATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGGGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCGACGTAAGAGTCGCTGTCGGTCGGATCATATgcgc                                                                                                             >  2:5982011/1‑150 (MQ=11)
                                           aaaGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCa                                                                                                            >  2:4313606/1‑150 (MQ=25)
                                               gtgtAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACac                                                                                                                                                      <  2:3474599/104‑2 (MQ=25)
                                               gtgtAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAg                                                                                                                                                      >  1:3474610/1‑104 (MQ=35)
                                                 gtAGGTCGGATAAGATGTGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATc                                                                                                      <  1:5447447/150‑1 (MQ=255)
                                                 gtAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATc                                                                                                      <  2:2063567/150‑1 (MQ=39)
                                                 gtAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATc                                                                                                      <  2:3372072/150‑1 (MQ=39)
                                                     gTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCGGATCCGGCCTGGAGGTGGGCAGCGGTTGAGTCACTGTCGGTCGGGTAAGATGCGCAGGGATCGCag                                                                                                  >  1:2780050/1‑149 (MQ=11)
                                                      tCGGATAAGATGCGCTAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGTCAACCGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACGGTCGGACGGATAAGATGCGCAAGTATCGCATc                                                                                                 <  2:2587512/150‑1 (MQ=11)
                                                      tCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAATATGCGCAAGTATCGCATc                                                                                                 >  1:1168958/1‑150 (MQ=37)
                                                      tCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATc                                                                                                 <  1:6656949/150‑1 (MQ=37)
                                                         gATAAGCTGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGa                                                                                              <  2:4040896/150‑1 (MQ=37)
                                                         gATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGa                                                                                              <  1:5919216/150‑1 (MQ=37)
                                                           tAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCGCTGGAGTTGGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGGTTGAGTCACTGTCGGTCGGATTAGATGCGCAAGGATCGCATCCggct                                                                                            >  2:5436937/1‑147 (MQ=11)
                                                            aaGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACat                                                                                           >  2:6876962/1‑150 (MQ=37)
                                                                  cgcAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGc                                                                                     <  1:393478/150‑1 (MQ=37)
                                                                  cgcAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGc                                                                                     <  2:6083792/150‑1 (MQ=37)
                                                                   gcAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCg                                                                                    <  1:3006565/150‑1 (MQ=37)
                                                                    cAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCgg                                                                                   <  1:5184855/150‑1 (MQ=37)
                                                                     aaGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGc                                                                                  >  1:3760294/1‑150 (MQ=37)
                                                                           cGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGa                                                                            <  1:4488815/150‑1 (MQ=37)
                                                                             cATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGt                                                                          <  2:5121219/150‑1 (MQ=37)
                                                                                 cGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTgg                                                                      <  2:7106811/150‑1 (MQ=37)
                                                                                  gACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGc                                                                     >  1:4205010/1‑150 (MQ=37)
                                                                                  gACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTAGCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGc                                                                     <  2:3188529/150‑1 (MQ=37)
                                                                                      ttattGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACa                                                                 >  1:6042782/1‑150 (MQ=37)
                                                                                           gCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGcc                                                            <  1:6876962/150‑1 (MQ=39)
                                                                                             ggCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCgg                                                          >  1:5330698/1‑150 (MQ=39)
                                                                                              gCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGa                                                         <  1:343068/150‑1 (MQ=39)
                                                                                                aCTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATg                                                       >  2:2267896/1‑150 (MQ=39)
                                                                                                aCTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATg                                                       <  1:2739012/150‑1 (MQ=39)
                                                                                                aCTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATg                                                       >  2:6619700/1‑150 (MQ=39)
                                                                                                 cTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGAAgc                                                      >  2:6228533/1‑150 (MQ=255)
                                                                                                     aGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCTCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATgcggcg                                                  >  1:6177280/1‑150 (MQ=21)
                                                                                                     aGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATgcggcg                                                  >  1:812880/1‑150 (MQ=39)
                                                                                                       tttGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGcgcg                                                >  2:1547777/1‑150 (MQ=39)
                                                                                                        ttGGCAACTGTGCCGGATGCGGCGCGAGCGCCGTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGa                                               <  2:4550368/150‑1 (MQ=255)
                                                                                                        ttGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGa                                               <  1:7058211/150‑1 (MQ=39)
                                                                                                          ggCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACAATATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAgc                                             >  1:2856819/1‑150 (MQ=255)
                                                                                                           gCAACATTTCCGGATGGGGCGCGATCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTAGGTCTGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTTGAGTTTGTCAACAGTGCCGGATGCGGCGCGAgcg                                            <  1:5860104/150‑1 (MQ=11)
                                                                                                            ccaccgggCCGGCTGCGGCGCGCGCGCCTGAGCCGGCCGCCCGGTGGGCCTCGTGTGAGTCCCTGTCGGTCGGATACGATGCGCAAGTATCGCCTCCGACATTATTGCGGCACGGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAgcgc                                           <  2:6133189/143‑1 (MQ=11)
                                                                                                            cAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACCGTTGGGCATCGTGTGAGTCACTGTCGGTCGGTTGAGTTGCGGTAGGATCGCGTCCGACATTATTGCGGCACGGGAGTGTGGCGCCCGGGCCGGAGGCGGCGCGGgcgc                                           >  2:5092665/1‑150 (MQ=11)
                                                                                                            cAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAgcgc                                           <  1:3074885/150‑1 (MQ=39)
                                                                                                            cAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAgcgc                                           <  2:38746/150‑1 (MQ=39)
                                                                                                                     cGGATGCGGCGCGAGCGCCTTCTCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCgg                                  <  2:6398008/150‑1 (MQ=255)
                                                                                                                      ggATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGc                                 <  1:1613341/150‑1 (MQ=39)
                                                                                                                            ggcgCGAGCGCCTTATCCGGCCTACAGTTGGGCATCTTTAGAGTCCCTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACgg                           <  2:5886263/150‑1 (MQ=255)
                                                                                                                            ggcgCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACgg                           >  1:4728954/1‑150 (MQ=40)
                                                                                                                            ggcgCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTCCgg                           >  2:4742619/1‑150 (MQ=2)
                                                                                                                            ggcgCGAGCGCCATATCCGGCCTACAGTTGGGCATCGTTTGAGACACTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGAGGCGCGAGCGCCTAATCCGGCCTACgt                           >  1:2515581/1‑149 (MQ=11)
                                                                                                                             gcgcGAGCGCCTTATCCGGCCTACCGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGCGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACgtt                          <  2:1085016/150‑3 (MQ=2)
                                                                                                                               gcgAGCGCCTTATGCGGCCTACAGGTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTg                        <  2:2002960/150‑1 (MQ=255)
                                                                                                                               gcgAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTg                        <  1:1394603/150‑1 (MQ=255)
                                                                                                                                cgAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTgg                       <  2:1630160/150‑1 (MQ=255)
                                                                                                                                     gcCTTATCCGGCCTACAGTTGGGCATCGTGTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACCTTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCCTc                  >  2:2768288/1‑150 (MQ=255)
                                                                                                                                      ccTTATCCGGCCTACAGTTGGGCATCTTGAGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCg                 <  1:1000088/150‑1 (MQ=255)
                                                                                                                                       catATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTCTCCGGCCTACGGTTGGGCATCGt                >  1:921720/3‑150 (MQ=255)
                                                                                                                                         tATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGttt              <  2:5706321/150‑1 (MQ=255)
                                                                                                                                             cGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTGTGAGt          >  1:2632736/1‑150 (MQ=255)
                                                                                                                                               gCCTACCGGGGGGCCGCGTGTGAGTCCCTGTCGGTCGGAGACGATGCGCAAGTCTCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCa        <  2:4453908/150‑1 (MQ=14)
                                                                                                                                                catACTGTTGGGCATCGTGTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCAc       <  2:4300475/148‑1 (MQ=255)
                                                                                                                                                     aGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAg  <  2:1174927/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCGTAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAG  >  NC_000913/4153298‑4153596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: