Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,612,329 A→G 5.2% intergenic (+40/‑82) ytjA → / → yjjU uncharacterized protein/putative patatin‑like family phospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,612,3290AG 5.2% 1246.8 / 37.0 364intergenic (+40/‑82)ytjA/yjjUuncharacterized protein/putative patatin‑like family phospholipase
Reads supporting (aligned to +/- strand):  ref base A (185/159);  new base G (14/5);  total (200/164)
Fisher's exact test for biased strand distribution p-value = 1.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01

Only 100 of 369 total aligned reads displayed.

TTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAGCCTGG  >  NC_000913/4612180‑4612478
                                                                                                                                                     |                                                                                                                                                     
 tGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAg                                                                                                                                                      <  2:4176278/150‑1 (MQ=255)
  gggTTTGGTGGTCGGGCCGGTACCGCTGCAGGCGCAGCTAACATTGTCTTTGGCGCCGGGATTATTCTGGTCCTGGTGAGTTTGTTCATGGGCCGAACACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGc                                                                                                                                                     <  2:5145605/150‑1 (MQ=255)
    gTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCgg                                                                                                                                                   >  2:5428472/1‑150 (MQ=255)
     ttttGTGGGTTGGCCGGTTCCGCTGATGGCGCTGCTAAATTTTTCTTTGTCTTCGGTATTTTTCTGTTCCTGTTTATTTTGTTAATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGa                                                                                                                                                  <  2:3192923/150‑1 (MQ=255)
          tggtCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGc                                                                                                                                             >  2:6340392/1‑150 (MQ=255)
          tggtCGGGCGGTTCCCGCTGCTGGCGCAGCTAAAATTGTCTTTGTCGTGGGGATTTTTCTGTTCGTGGTGAGTTTGTTCATGGGCCCAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGc                                                                                                                                             <  1:6485243/150‑1 (MQ=255)
              cgggCCGGTACCGCGGCCGGCGCAGCTAAAATGGTCTTTGTCGGCGGGATTATTCTGTTCCGGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCtttt                                                                                                                                         <  2:1818578/148‑1 (MQ=255)
               tGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCTGTCCAGCGGACTGGCTTTTg                                                                                                                                        >  1:2885024/1‑150 (MQ=255)
               tGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTg                                                                                                                                        >  1:7188486/1‑150 (MQ=255)
                ggCCGGTACCGCTGCCGGCGCAGATAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTGGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGc                                                                                                                                       <  2:6243879/150‑1 (MQ=255)
                 gCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCg                                                                                                                                      >  1:2967468/1‑150 (MQ=255)
                 gCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCg                                                                                                                                      >  1:3008584/1‑150 (MQ=255)
                   cGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGt                                                                                                                                    >  2:3830521/1‑150 (MQ=255)
                         cGCTGCCGGCGCAGCTAAAATTGTCTGTGACGTCGGGATTATTCTGTTCCTGGTGAGTTTGTGCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGc                                                                                                                              <  1:706245/150‑1 (MQ=255)
                         cGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGTTGAGTTTTTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGc                                                                                                                              <  2:5503587/150‑1 (MQ=255)
                         cGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTGTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGGTTTAGc                                                                                                                              >  2:4732195/1‑150 (MQ=255)
                         cGCTGCAGGCGCAGATAAATTTGTCTTTGTCGTGGGGATTATTCTGTTCGTGGTGTGTTTGTTCCGGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGc                                                                                                                              <  1:3591690/150‑1 (MQ=255)
                           cTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGa                                                                                                                            >  1:5760437/1‑150 (MQ=255)
                            tGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGGTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGaa                                                                                                                           >  2:3712302/1‑150 (MQ=255)
                                 gcgcAGCTAAACTTGTCTTTGTGGTGGGTTTTTTTCTTTTCCTGGTTTTTTTGTTAATGGGCCCAACACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGACAGTCCAGCGGACTGGCATTTGCGGTTTTAGCGAataata                                                                                                                      <  1:1297369/150‑1 (MQ=255)
                                 gcgcAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAataata                                                                                                                      >  2:2732879/1‑150 (MQ=255)
                                  cgcTGCTAAATTTGTCTTTGTGGTCGGGATTATTCTGTTCCTGGTGTGTTTTTTCTTGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAAtaataa                                                                                                                     <  2:694177/150‑1 (MQ=255)
                                       cTAAATTTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGTGTTTGTTCAGGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGc                                                                                                                <  2:1865481/150‑1 (MQ=255)
                                       atAAATTGGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTTAGTTTGTTCATGGGCCTAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGc                                                                                                                <  1:6678975/149‑1 (MQ=255)
                                        tAAAATTGTCTTTGTCGTCGGTTTTTTTCTTTTCCTGGTTAGTTTGTTCTTGGGCCGAAAAAGACCCTAGATTACAGACCATTATGAATATTTTATCCAAAGCGAGTCCAGCGGAGTGGCTTTTGCGGTTTTAGCGAATAATAAATTGct                                                                                                               <  2:2878729/150‑2 (MQ=255)
                                        tAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCg                                                                                                               >  1:1858286/1‑150 (MQ=255)
                                        aaaaaTTGTCTTTGTCGGCGGGATTATTCTGTTCCTGGTGAGTTTGTCCATGGGCCCAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCg                                                                                                               <  1:6330933/149‑1 (MQ=255)
                                                tgtttGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGTCCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGATTAATAAATTGCGTTACTTTg                                                                                                       >  1:4513501/3‑150 (MQ=255)
                                                tCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTg                                                                                                       >  1:872911/1‑150 (MQ=255)
                                                 cTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGt                                                                                                      >  1:4108289/1‑150 (MQ=255)
                                                  tttGTCGTGGGGATTATTCTGTCCCTGGTGATTTGGTTCTGGGGCCTAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTc                                                                                                     <  1:5548306/150‑1 (MQ=255)
                                                  tttGGCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTc                                                                                                     <  2:3496249/150‑1 (MQ=255)
                                                           ggtattattCTGTTCCTGGTGAGTTTTTTCTTGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTca                                                                                            <  1:2619624/147‑1 (MQ=255)
                                                           gggATTATTCTGTTCCTGGTTAGTTTGTTCTTGGGCCTAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTca                                                                                            <  2:4946055/150‑1 (MQ=255)
                                                           gggATTATTCTGTTCCTGGTGATTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTca                                                                                            <  1:2708278/150‑1 (MQ=255)
                                                           gggATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTca                                                                                            <  1:1685172/150‑1 (MQ=255)
                                                             gATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTcaca                                                                                          >  1:6843626/1‑150 (MQ=255)
                                                              attattCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCacaa                                                                                         <  2:5862832/150‑1 (MQ=255)
                                                              attattCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCacaa                                                                                         >  1:340847/1‑150 (MQ=255)
                                                                     tGTTCCTGTTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAAc                                                                                  <  2:1656976/150‑1 (MQ=255)
                                                                     tGTTCCTGGTGAGTTTTTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAATATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAAc                                                                                  <  1:1812778/150‑1 (MQ=255)
                                                                     tGTTCCTGGTGAGTTTGTTCATGGGCCGTAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAAc                                                                                  <  2:7110263/150‑1 (MQ=255)
                                                                       ttCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAg                                                                                >  1:2796121/1‑150 (MQ=255)
                                                                          cTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGac                                                                             >  2:3106542/1‑149 (MQ=255)
                                                                          cTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGaa                                                                             <  2:4841686/150‑1 (MQ=255)
                                                                           tGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCTGACCATTAAGAAAATTTTATCCAATGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAATTTGCGTTACTTTGTCATTCTTTGACAACAAAAACAGGAAg                                                                            >  1:105230/1‑150 (MQ=255)
                                                                           tGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAATCAGGAAg                                                                            >  1:6079741/1‑150 (MQ=255)
                                                                           tGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAg                                                                            >  2:794790/1‑150 (MQ=255)
                                                                           tGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTCATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAg                                                                            >  2:1588668/1‑150 (MQ=255)
                                                                                     gtccATGGGCCGAACACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCATTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTgg                                                                  <  2:6127616/147‑1 (MQ=255)
                                                                                     gtacATGGGCCCAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTgg                                                                  <  1:6920316/147‑1 (MQ=255)
                                                                                     gTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTgg                                                                  <  1:1218378/150‑1 (MQ=255)
                                                                                     gTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTgg                                                                  <  1:322078/150‑1 (MQ=255)
                                                                                     gTTCATGGGCCGAAAACGACACTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTCCGGTATTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTgg                                                                  >  2:4313757/1‑150 (MQ=255)
                                                                                         aTGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAAAAAATTGCGTTACTTTGTCATTCTTTGACAACAAAAAAAGGAAGGGGGAGGTGGGGCa                                                              >  2:4876418/1‑150 (MQ=255)
                                                                                          tGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAg                                                             >  2:3672655/1‑150 (MQ=255)
                                                                                          tGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAAAAAAAACAGGAAGGCAGAGGTGGGGCAg                                                             >  2:3388363/1‑150 (MQ=255)
                                                                                          tGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTGTTGCGGTTTTAGCGAAAAATAAATTGAGATACTTTGTCATTCTTTCACAACAAAAACAAGAAGGCAGAGGTGGGGccg                                                             >  2:3732839/1‑148 (MQ=255)
                                                                                          tGGGCCGAAAACGACCCGAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAg                                                             >  1:5064414/1‑150 (MQ=255)
                                                                                          tGGGCCGAAAAAGACCCTAGATTTCAGACAATTAAGAAAATAATATCCAAACCCAGTCCAGCGGACTGGCTTTTGCGGATATAGCGAATAATAAATTGAGTTACTTTGTCATTCTTTCACAAAAAAAACAGTAAGGCAGAGGTGGGGCAg                                                             >  2:4661961/1‑150 (MQ=255)
                                                                                           gggCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGc                                                            <  2:6771598/150‑1 (MQ=255)
                                                                                             gCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGa                                                          >  1:5247470/1‑150 (MQ=255)
                                                                                             gCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGa                                                          >  2:1969436/1‑150 (MQ=255)
                                                                                              cctaaaaCGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGaa                                                         <  2:2176336/147‑1 (MQ=255)
                                                                                               cGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAAt                                                        >  2:2149967/1‑150 (MQ=255)
                                                                                                   aaCGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCt                                                    <  1:3832401/150‑1 (MQ=255)
                                                                                                   aaCGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCt                                                    <  1:2448426/150‑1 (MQ=255)
                                                                                                   aaCGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCt                                                    <  1:6732630/150‑1 (MQ=255)
                                                                                                       aCCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCATTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTaa                                                >  2:937342/1‑150 (MQ=255)
                                                                                                        cccTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAAc                                               <  2:2499251/150‑1 (MQ=255)
                                                                                                         ccTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACg                                              >  2:3932856/1‑150 (MQ=255)
                                                                                                          cTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGc                                             >  2:6854853/1‑150 (MQ=255)
                                                                                                           tAGATTTCAGACCATTAAGAAAATTTTATCCATAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCt                                            >  1:2095822/1‑150 (MQ=255)
                                                                                                           tAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCt                                            >  2:5632200/1‑150 (MQ=255)
                                                                                                             gATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTg                                          <  1:6026014/150‑1 (MQ=255)
                                                                                                                     aCCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATAtt                                  <  1:6252408/150‑1 (MQ=255)
                                                                                                                       cATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTgc                                <  2:5805103/150‑1 (MQ=255)
                                                                                                                          tAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGcc                             <  1:4444047/150‑1 (MQ=255)
                                                                                                                             gAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGtt                          >  2:2124542/1‑150 (MQ=255)
                                                                                                                             gAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGtt                          >  2:1058515/1‑150 (MQ=255)
                                                                                                                              aaaaTTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTg                         >  2:6549339/1‑150 (MQ=255)
                                                                                                                                  ttttATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTTTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGAGCCGGTGTCGc                     >  1:5915235/1‑150 (MQ=255)
                                                                                                                                  ttttATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGc                     >  1:6402737/1‑150 (MQ=255)
                                                                                                                                   tttATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCt                    <  2:113539/150‑1 (MQ=255)
                                                                                                                                     tATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGTTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTaa                  >  2:2660463/1‑150 (MQ=255)
                                                                                                                                     tATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTaa                  <  1:4655532/150‑1 (MQ=255)
                                                                                                                                         cAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGcc              <  1:65917/150‑1 (MQ=255)
                                                                                                                                          aaaGCCAGTCCGCTGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCGGTAACGCTGGGTAATATTGCGCCGTTGTCGCTAAGGCCg             >  1:2328336/1‑150 (MQ=255)
                                                                                                                                          aaaGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCg             >  2:531886/1‑150 (MQ=255)
                                                                                                                                          aaaGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCg             >  1:4767721/1‑150 (MQ=255)
                                                                                                                                          aaaGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCg             >  2:1471258/1‑150 (MQ=255)
                                                                                                                                          aaaGCCAGTCCAGCGGACTGGCTTTTGCGGTGTTAGCGAATAATACATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTACCGCTTGGTAAGATTGCGCCGTTGGCGCTAAGGCCg             >  2:4485494/1‑150 (MQ=255)
                                                                                                                                           aaGCCCGTCCGCTGGACTGGCTTTTGCGGTTGTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCCGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTCATATTGCGCCGGTGTCGCTAAGGCCGt            >  2:1267616/1‑150 (MQ=255)
                                                                                                                                             gCCAGTCCGCTGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCCGAGGTGGGGCAGCGAATACCTGTAACGCTGGGTCATATTGCGCCGTTGTCGCTACGGCCGTTc          >  2:672208/1‑150 (MQ=255)
                                                                                                                                             gCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTATTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGACTAACTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGGTc          >  1:2515520/1‑150 (MQ=255)
                                                                                                                                             gCCAGTCCAGCGGACTGGCTTTTGCGGGTTTAGCGAATAATAAATTGCGTTTCTTTGTCATTCTTTCACAACAAAAACAGGAGGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTc          >  1:1531375/1‑150 (MQ=255)
                                                                                                                                                 gTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAGc      <  1:5512183/150‑1 (MQ=255)
                                                                                                                                                 gTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAGc      >  1:5379543/1‑150 (MQ=255)
                                                                                                                                                     aGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAGCCTgg  >  2:5932963/1‑150 (MQ=255)
                                                                                                                                                     aGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAGCCTgg  >  1:144703/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAGCCTGG  >  NC_000913/4612180‑4612478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: