Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,612,330 G→C 5.3% intergenic (+41/‑81) ytjA → / → yjjU uncharacterized protein/putative patatin‑like family phospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,612,3300GC 5.3% 1335.6 / 47.0 366intergenic (+41/‑81)ytjA/yjjUuncharacterized protein/putative patatin‑like family phospholipase
Reads supporting (aligned to +/- strand):  ref base G (188/157);  new base C (15/5);  total (204/162)
Fisher's exact test for biased strand distribution p-value = 1.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 369 total aligned reads displayed.

TGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAGCCTGGA  >  NC_000913/4612181‑4612479
                                                                                                                                                     |                                                                                                                                                     
tGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAg                                                                                                                                                       <  2:4176278/150‑1 (MQ=255)
 gggTTTGGTGGTCGGGCCGGTACCGCTGCAGGCGCAGCTAACATTGTCTTTGGCGCCGGGATTATTCTGGTCCTGGTGAGTTTGTTCATGGGCCGAACACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGc                                                                                                                                                      <  2:5145605/150‑1 (MQ=255)
   gTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCgg                                                                                                                                                    >  2:5428472/1‑150 (MQ=255)
    ttttGTGGGTTGGCCGGTTCCGCTGATGGCGCTGCTAAATTTTTCTTTGTCTTCGGTATTTTTCTGTTCCTGTTTATTTTGTTAATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGa                                                                                                                                                   <  2:3192923/150‑1 (MQ=255)
         tggtCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGc                                                                                                                                              >  2:6340392/1‑150 (MQ=255)
         tggtCGGGCGGTTCCCGCTGCTGGCGCAGCTAAAATTGTCTTTGTCGTGGGGATTTTTCTGTTCGTGGTGAGTTTGTTCATGGGCCCAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGc                                                                                                                                              <  1:6485243/150‑1 (MQ=255)
            tCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCttt                                                                                                                                           >  2:1778005/1‑150 (MQ=255)
              tGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCTGTCCAGCGGACTGGCTTTTg                                                                                                                                         >  1:2885024/1‑150 (MQ=255)
              tGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCGCTGGACTGGCtttgg                                                                                                                                         <  2:2306716/150‑3 (MQ=255)
               ggCCGGTACCGCTGCCGGCGCAGATAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTGGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGc                                                                                                                                        <  2:6243879/150‑1 (MQ=255)
                gCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCg                                                                                                                                       >  1:2967468/1‑150 (MQ=255)
                gCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCg                                                                                                                                       >  1:3008584/1‑150 (MQ=255)
                  cGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGt                                                                                                                                     >  2:1877751/1‑150 (MQ=255)
                  cGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGt                                                                                                                                     >  2:3830521/1‑150 (MQ=255)
                      aCCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTa                                                                                                                                 >  2:2129026/1‑150 (MQ=255)
                       ccGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAg                                                                                                                                >  2:2029116/1‑150 (MQ=255)
                        cgcgGCAGGCGCCTCTAAAATTGTCTTTGCCGTCGGTATTTTTCTTTTCCTGGTGATTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGc                                                                                                                               <  1:723638/150‑1 (MQ=255)
                        cGCTGCCGGCGCAGCTAAAATTGTCTGTGACGTCGGGATTATTCTGTTCCTGGTGAGTTTGTGCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGc                                                                                                                               <  1:706245/150‑1 (MQ=255)
                        cGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGTTGAGTTTTTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGc                                                                                                                               <  2:5503587/150‑1 (MQ=255)
                        cGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTGTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGGTTTAGc                                                                                                                               >  2:4732195/1‑150 (MQ=255)
                        cGCTGCAGGCGCAGATAAATTTGTCTTTGTCGTGGGGATTATTCTGTTCGTGGTGTGTTTGTTCCGGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGc                                                                                                                               <  1:3591690/150‑1 (MQ=255)
                          cTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGa                                                                                                                             >  1:5760437/1‑150 (MQ=255)
                           tGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGGTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGaa                                                                                                                            >  2:3712302/1‑150 (MQ=255)
                                gcgcAGCTAAACTTGTCTTTGTGGTGGGTTTTTTTCTTTTCCTGGTTTTTTTGTTAATGGGCCCAACACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGACAGTCCAGCGGACTGGCATTTGCGGTTTTAGCGAataata                                                                                                                       <  1:1297369/150‑1 (MQ=255)
                                 cgcTGCTAAATTTGTCTTTGTGGTCGGGATTATTCTGTTCCTGGTGTGTTTTTTCTTGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAAtaataa                                                                                                                      <  2:694177/150‑1 (MQ=255)
                                      atAAATTGGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTTAGTTTGTTCATGGGCCTAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGc                                                                                                                 <  1:6678975/149‑1 (MQ=255)
                                       tAAAATTGTCTTTGTCGTCGGTTTTTTTCTTTTCCTGGTTAGTTTGTTCTTGGGCCGAAAAAGACCCTAGATTACAGACCATTATGAATATTTTATCCAAAGCGAGTCCAGCGGAGTGGCTTTTGCGGTTTTAGCGAATAATAAATTGct                                                                                                                <  2:2878729/150‑2 (MQ=255)
                                       tAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCg                                                                                                                >  1:1858286/1‑150 (MQ=255)
                                       aaaaaTTGTCTTTGTCGGCGGGATTATTCTGTTCCTGGTGAGTTTGTCCATGGGCCCAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCg                                                                                                                <  1:6330933/149‑1 (MQ=255)
                                            ttGTCTTTGTCGTCGGGATTATTCTGTCCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACt                                                                                                           <  2:2756177/150‑1 (MQ=255)
                                               tgtttGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGTCCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGATTAATAAATTGCGTTACTTTg                                                                                                        >  1:4513501/3‑150 (MQ=255)
                                                cTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGt                                                                                                       >  1:4108289/1‑150 (MQ=255)
                                                 tttGTCGTGGGGATTATTCTGTCCCTGGTGATTTGGTTCTGGGGCCTAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTc                                                                                                      <  1:5548306/150‑1 (MQ=255)
                                                 tttGGCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTc                                                                                                      <  2:3496249/150‑1 (MQ=255)
                                                          ggtattattCTGTTCCTGGTGAGTTTTTTCTTGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTca                                                                                             <  1:2619624/147‑1 (MQ=255)
                                                          gggATTATTCTGTTCCTGGTTAGTTTGTTCTTGGGCCTAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTca                                                                                             <  2:4946055/150‑1 (MQ=255)
                                                          gggATTATTCTGTTCCTGGTGATTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTca                                                                                             <  1:2708278/150‑1 (MQ=255)
                                                          gggATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTca                                                                                             <  1:1685172/150‑1 (MQ=255)
                                                            gATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTcaca                                                                                           >  1:6843626/1‑150 (MQ=255)
                                                             attattCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCacaa                                                                                          <  2:5862832/150‑1 (MQ=255)
                                                             attattCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCacaa                                                                                          >  1:340847/1‑150 (MQ=255)
                                                                    tGTTCCTGGTGAGTTTTTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAATATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAAc                                                                                   <  1:1812778/150‑1 (MQ=255)
                                                                    tGTTCCTGGTGAGTTTGTTCATGGGCCGTAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAAc                                                                                   <  2:7110263/150‑1 (MQ=255)
                                                                      ttCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAg                                                                                 >  1:2796121/1‑150 (MQ=255)
                                                                        cgtGGTGAGTTTTTTCTTGGGCCTAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGa                                                                               <  2:2703073/148‑1 (MQ=255)
                                                                         cTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGac                                                                              >  2:3106542/1‑149 (MQ=255)
                                                                         cTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGaa                                                                              <  2:4841686/150‑1 (MQ=255)
                                                                          tGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCTGACCATTAAGAAAATTTTATCCAATGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAATTTGCGTTACTTTGTCATTCTTTGACAACAAAAACAGGAAg                                                                             >  1:105230/1‑150 (MQ=255)
                                                                          tGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAATCAGGAAg                                                                             >  1:6079741/1‑150 (MQ=255)
                                                                          tGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAg                                                                             >  2:794790/1‑150 (MQ=255)
                                                                                    gtccATGGGCCGAACACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCATTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTgg                                                                   <  2:6127616/147‑1 (MQ=255)
                                                                                    gtacATGGGCCCAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTgg                                                                   <  1:6920316/147‑1 (MQ=255)
                                                                                    gTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTgg                                                                   <  1:1218378/150‑1 (MQ=255)
                                                                                    gTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTgg                                                                   <  1:322078/150‑1 (MQ=255)
                                                                                    gTTCATGGGCCGAAAACGACACTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTCCGGTATTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTgg                                                                   >  2:4313757/1‑150 (MQ=255)
                                                                                      tCATGGGCCGACCACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATATATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTgggg                                                                 <  1:935873/150‑1 (MQ=255)
                                                                                        aTGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAAAAAATTGCGTTACTTTGTCATTCTTTGACAACAAAAAAAGGAAGGGGGAGGTGGGGCa                                                               >  2:4876418/1‑150 (MQ=255)
                                                                                         tGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAg                                                              >  2:3672655/1‑150 (MQ=255)
                                                                                         tGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAAAAAAAACAGGAAGGCAGAGGTGGGGCAg                                                              >  2:3388363/1‑150 (MQ=255)
                                                                                         tGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTGTTGCGGTTTTAGCGAAAAATAAATTGAGATACTTTGTCATTCTTTCACAACAAAAACAAGAAGGCAGAGGTGGGGccg                                                              >  2:3732839/1‑148 (MQ=255)
                                                                                         tGGGCCGAAAACGACCCGAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAg                                                              >  1:5064414/1‑150 (MQ=255)
                                                                                         tGGGCCGAAAAAGACCCTAGATTTCAGACAATTAAGAAAATAATATCCAAACCCAGTCCAGCGGACTGGCTTTTGCGGATATAGCGAATAATAAATTGAGTTACTTTGTCATTCTTTCACAAAAAAAACAGTAAGGCAGAGGTGGGGCAg                                                              >  2:4661961/1‑150 (MQ=255)
                                                                                          gggCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGc                                                             <  2:6771598/150‑1 (MQ=255)
                                                                                            gCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGa                                                           >  1:5247470/1‑150 (MQ=255)
                                                                                               gAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATa                                                        <  2:2632645/150‑1 (MQ=255)
                                                                                               gAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATa                                                        <  2:1829251/150‑1 (MQ=255)
                                                                                                  aaCGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCt                                                     <  1:3832401/150‑1 (MQ=255)
                                                                                                  aaCGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCt                                                     <  1:2448426/150‑1 (MQ=255)
                                                                                                  aaCGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCt                                                     <  1:6732630/150‑1 (MQ=255)
                                                                                                    cGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGt                                                   >  2:1321091/1‑150 (MQ=255)
                                                                                                      aCCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCATTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTaa                                                 >  2:937342/1‑150 (MQ=255)
                                                                                                        ccTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACg                                               >  2:3932856/1‑150 (MQ=255)
                                                                                                         cTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGc                                              >  2:6854853/1‑150 (MQ=255)
                                                                                                          tAGATTTCAGACCATTAAGAAAATTTTATCCATAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCt                                             >  1:2095822/1‑150 (MQ=255)
                                                                                                          tAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCt                                             >  2:5632200/1‑150 (MQ=255)
                                                                                                            gATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTg                                           <  1:6026014/150‑1 (MQ=255)
                                                                                                               ttCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTa                                        >  2:1609889/1‑150 (MQ=255)
                                                                                                                    aCCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATAtt                                   <  1:6252408/150‑1 (MQ=255)
                                                                                                                      cATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTgc                                 <  2:5805103/150‑1 (MQ=255)
                                                                                                                         tAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGcc                              <  1:4444047/150‑1 (MQ=255)
                                                                                                                             aaaaTTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTg                          >  2:6549339/1‑150 (MQ=255)
                                                                                                                                 ttttATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTTTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGAGCCGGTGTCGc                      >  1:5915235/1‑150 (MQ=255)
                                                                                                                                 ttttATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGc                      >  1:6402737/1‑150 (MQ=255)
                                                                                                                                  tttATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCt                     <  2:113556/150‑1 (MQ=255)
                                                                                                                                    tATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTaa                   <  1:4655532/150‑1 (MQ=255)
                                                                                                                                    tATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTaa                   <  2:2158126/150‑1 (MQ=255)
                                                                                                                                       caaaaGCCAGTCCGCTGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGc                >  2:1115985/3‑150 (MQ=255)
                                                                                                                                        cAAAGCCCGTCCAGCGGCCTGGCGGGTGCGGTGTTAGCGAATACTAAATTGCGGTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGACTACCTGTAACGCGGGGTCATATTGCGCCGGTGTCGCTCAGGcc               >  2:1484011/1‑150 (MQ=255)
                                                                                                                                        cAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGcc               <  1:65917/150‑1 (MQ=255)
                                                                                                                                         aaaGCCAGTCCGCTGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCGGTAACGCTGGGTAATATTGCGCCGTTGTCGCTAAGGCCg              >  1:2328336/1‑150 (MQ=255)
                                                                                                                                         aaaGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCg              >  1:4767721/1‑150 (MQ=255)
                                                                                                                                         aaaGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCg              >  2:531886/1‑150 (MQ=255)
                                                                                                                                         aaaGCCAGTCCAGCGGACTGGCTTTTGCGGTGTTAGCGAATAATACATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTACCGCTTGGTAAGATTGCGCCGTTGGCGCTAAGGCCg              >  2:4485494/1‑150 (MQ=255)
                                                                                                                                            gCCAGTCCGCTGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCCGAGGTGGGGCAGCGAATACCTGTAACGCTGGGTCATATTGCGCCGTTGTCGCTACGGCCGTTc           >  2:672208/1‑150 (MQ=255)
                                                                                                                                            gCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTATTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGACTAACTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGGTc           >  1:2515520/1‑150 (MQ=255)
                                                                                                                                            gCCAGTCCAGCGGACTGGCTTTTGCGGGTTTAGCGAATAATAAATTGCGTTTCTTTGTCATTCTTTCACAACAAAAACAGGAGGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTc           >  1:1531375/1‑150 (MQ=255)
                                                                                                                                                gTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAGc       >  1:5379543/1‑150 (MQ=255)
                                                                                                                                                gTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAGc       <  1:5512183/150‑1 (MQ=255)
                                                                                                                                                    aGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAGCCTgg   >  2:5932963/1‑150 (MQ=255)
                                                                                                                                                    aGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAGCCTgg   >  1:144703/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAGCCTGGA  >  NC_000913/4612181‑4612479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: