Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,612,331 C→T 5.5% intergenic (+42/‑80) ytjA → / → yjjU uncharacterized protein/putative patatin‑like family phospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,612,3310CT 5.5% 1264.8 / 37.6 364intergenic (+42/‑80)ytjA/yjjUuncharacterized protein/putative patatin‑like family phospholipase
Reads supporting (aligned to +/- strand):  ref base C (190/154);  new base T (14/6);  total (204/160)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 367 total aligned reads displayed.

GGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAGCCTGGA  >  NC_000913/4612182‑4612479
                                                                                                                                                     |                                                                                                                                                    
  gTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCgg                                                                                                                                                    >  2:5428472/1‑150 (MQ=255)
   ttttGTGGGTTGGCCGGTTCCGCTGATGGCGCTGCTAAATTTTTCTTTGTCTTCGGTATTTTTCTGTTCCTGTTTATTTTGTTAATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGa                                                                                                                                                   <  2:3192923/150‑1 (MQ=255)
      ggtggtCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTg                                                                                                                                                >  2:3470689/1‑150 (MQ=255)
        tggtCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGc                                                                                                                                              >  2:6340392/1‑150 (MQ=255)
        tggtCGGGCGGTTCCCGCTGCTGGCGCAGCTAAAATTGTCTTTGTCGTGGGGATTTTTCTGTTCGTGGTGAGTTTGTTCATGGGCCCAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGc                                                                                                                                              <  1:6485243/150‑1 (MQ=255)
            cgggCCGGTACCGCGGCCGGCGCAGCTAAAATGGTCTTTGTCGGCGGGATTATTCTGTTCCGGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCtttt                                                                                                                                          <  2:1818578/148‑1 (MQ=255)
             tGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCTGTCCAGCGGACTGGCTTTTg                                                                                                                                         >  1:2885024/1‑150 (MQ=255)
             tGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTg                                                                                                                                         >  1:7188486/1‑150 (MQ=255)
              ggCCGGTACCGCTGCCGGCGCAGATAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTGGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGc                                                                                                                                        <  2:6243879/150‑1 (MQ=255)
               gCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCg                                                                                                                                       >  1:3008584/1‑150 (MQ=255)
                 cGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGt                                                                                                                                     >  2:3830521/1‑150 (MQ=255)
                       cGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTGTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGGTTTAGc                                                                                                                               >  2:4732195/1‑150 (MQ=255)
                       cGCTGCAGGCGCAGATAAATTTGTCTTTGTCGTGGGGATTATTCTGTTCGTGGTGTGTTTGTTCCGGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGc                                                                                                                               <  1:3591690/150‑1 (MQ=255)
                          tGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGGTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGaa                                                                                                                            >  2:3712302/1‑150 (MQ=255)
                               gcgcAGCTAAACTTGTCTTTGTGGTGGGTTTTTTTCTTTTCCTGGTTTTTTTGTTAATGGGCCCAACACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGACAGTCCAGCGGACTGGCATTTGCGGTTTTAGCGAataata                                                                                                                       <  1:1297369/150‑1 (MQ=255)
                                cgcTGCTAAATTTGTCTTTGTGGTCGGGATTATTCTGTTCCTGGTGTGTTTTTTCTTGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAAtaataa                                                                                                                      <  2:694177/150‑1 (MQ=255)
                                     cTAAATTTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGTGTTTGTTCAGGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGc                                                                                                                 <  2:1865481/150‑1 (MQ=255)
                                     cTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCTAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGc                                                                                                                 <  2:4566510/150‑1 (MQ=255)
                                      tAAAATTGTCTTTGTCGTCGGTTTTTTTCTTTTCCTGGTTAGTTTGTTCTTGGGCCGAAAAAGACCCTAGATTACAGACCATTATGAATATTTTATCCAAAGCGAGTCCAGCGGAGTGGCTTTTGCGGTTTTAGCGAATAATAAATTGct                                                                                                                <  2:2878729/150‑2 (MQ=255)
                                      tAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCg                                                                                                                >  1:1858286/1‑150 (MQ=255)
                                      tAAAATTGTATTTGTCGTCGGTATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCg                                                                                                                <  1:6330890/150‑1 (MQ=255)
                                       aaatTTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAACACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGt                                                                                                               <  1:459877/150‑1 (MQ=255)
                                         aaTTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTa                                                                                                             >  1:6589352/1‑150 (MQ=255)
                                           ttGTCTTTGTCGTCGGGATTATTCTGTCCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACt                                                                                                           <  2:2756177/150‑1 (MQ=255)
                                              tgtttGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGTCCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGATTAATAAATTGCGTTACTTTg                                                                                                        >  1:4513501/3‑150 (MQ=255)
                                              tCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTg                                                                                                        >  1:872911/1‑150 (MQ=255)
                                                      gtcgGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCtt                                                                                                <  2:4127211/150‑1 (MQ=255)
                                                      gtcgGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCtt                                                                                                >  1:6642311/1‑150 (MQ=255)
                                                       tcgGTATTTTTCTGTTCCTGGTGTGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCttt                                                                                               <  2:2149809/150‑1 (MQ=255)
                                                         ggtattattCTGTTCCTGGTGAGTTTTTTCTTGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTca                                                                                             <  1:2619624/147‑1 (MQ=255)
                                                         gggATTATTCTGTTCCTGGTTAGTTTGTTCTTGGGCCTAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTca                                                                                             <  2:4946055/150‑1 (MQ=255)
                                                         gggATTATTCTGTTCCTGGTGATTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTca                                                                                             <  1:2708278/150‑1 (MQ=255)
                                                         gggATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTca                                                                                             <  1:1685172/150‑1 (MQ=255)
                                                            attattCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCacaa                                                                                          <  2:5862832/150‑1 (MQ=255)
                                                                  cTGTTCCTGGTGAGTTTGTTCAGGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACaaaaa                                                                                    <  1:6983803/150‑1 (MQ=255)
                                                                   tGTTCCTGTTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAAc                                                                                   <  2:1656976/150‑1 (MQ=255)
                                                                   tGTTCCTGGTGAGTTTTTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAATATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAAc                                                                                   <  1:1812778/150‑1 (MQ=255)
                                                                   tGTTCCTGGTGAGTTTGTTCATGGGCCGTAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAAc                                                                                   <  2:7110263/150‑1 (MQ=255)
                                                                     ttCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAg                                                                                 >  1:2796121/1‑150 (MQ=255)
                                                                      tCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAgg                                                                                >  1:409716/1‑150 (MQ=255)
                                                                        cTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGaa                                                                              >  1:571296/1‑150 (MQ=255)
                                                                         tGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCTGACCATTAAGAAAATTTTATCCAATGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAATTTGCGTTACTTTGTCATTCTTTGACAACAAAAACAGGAAg                                                                             >  1:105230/1‑150 (MQ=255)
                                                                         tGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAg                                                                             >  2:794790/1‑150 (MQ=255)
                                                                         tGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAg                                                                             >  1:6005989/1‑150 (MQ=255)
                                                                         tGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTCATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAg                                                                             >  2:1588668/1‑150 (MQ=255)
                                                                                   gtccATGGGCCGAACACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCATTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTgg                                                                   <  2:6127616/147‑1 (MQ=255)
                                                                                   ggtCAGGGGCCGAACACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTgg                                                                   <  2:5527868/148‑1 (MQ=255)
                                                                                   gTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTgg                                                                   <  1:1218378/150‑1 (MQ=255)
                                                                                   gTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTgg                                                                   <  1:322078/150‑1 (MQ=255)
                                                                                   gTTCATGGGCCGAAAACGACACTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTCCGGTATTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTgg                                                                   >  2:4313757/1‑150 (MQ=255)
                                                                                    ttCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTggg                                                                  >  1:6919100/1‑150 (MQ=255)
                                                                                       aTGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAAAAAATTGCGTTACTTTGTCATTCTTTGACAACAAAAAAAGGAAGGGGGAGGTGGGGCa                                                               >  2:4876418/1‑150 (MQ=255)
                                                                                        tGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAg                                                              >  2:3672655/1‑150 (MQ=255)
                                                                                        tGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAAAAAAAACAGGAAGGCAGAGGTGGGGCAg                                                              >  2:3388363/1‑150 (MQ=255)
                                                                                        tGGGCCGAAAACGACCCGAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAg                                                              >  1:5064414/1‑150 (MQ=255)
                                                                                        tGGGCCGAAAAAGACCCTAGATTTCAGACAATTAAGAAAATAATATCCAAACCCAGTCCAGCGGACTGGCTTTTGCGGATATAGCGAATAATAAATTGAGTTACTTTGTCATTCTTTCACAAAAAAAACAGTAAGGCAGAGGTGGGGCAg                                                              >  2:4661961/1‑150 (MQ=255)
                                                                                         gggCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGc                                                             <  2:6771598/150‑1 (MQ=255)
                                                                                           gCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGa                                                           >  2:1969436/1‑150 (MQ=255)
                                                                                            cctaaaaCGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGaa                                                          <  2:2176336/147‑1 (MQ=255)
                                                                                              gAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATa                                                        <  2:2632645/150‑1 (MQ=255)
                                                                                                 caCGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGTGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCt                                                     <  2:3723688/149‑1 (MQ=255)
                                                                                                 aaCGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCt                                                     <  1:3832401/150‑1 (MQ=255)
                                                                                                 aaCGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCt                                                     <  1:2448426/150‑1 (MQ=255)
                                                                                                 aaCGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCt                                                     <  1:6732630/150‑1 (MQ=255)
                                                                                                     aCCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCATTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTaa                                                 >  2:937342/1‑150 (MQ=255)
                                                                                                       ccTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCt                                                     <  2:484237/144‑1 (MQ=255)
                                                                                                       ccTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACg                                               >  2:3932856/1‑150 (MQ=255)
                                                                                                        cTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGc                                              >  2:6854853/1‑150 (MQ=255)
                                                                                                         tAGATTTCAGACCATTAAGAAAATTTTATCCATAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCt                                             >  1:2095822/1‑150 (MQ=255)
                                                                                                         tAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCt                                             >  2:5632200/1‑150 (MQ=255)
                                                                                                           gATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTg                                           <  1:6373630/150‑1 (MQ=255)
                                                                                                                   aCCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATAtt                                   <  1:6252408/150‑1 (MQ=255)
                                                                                                                     cATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTgc                                 <  2:5805103/150‑1 (MQ=255)
                                                                                                                     cATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTgc                                 <  1:5539236/150‑1 (MQ=255)
                                                                                                                      aTTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTgcg                                <  2:3076669/150‑1 (MQ=255)
                                                                                                                        tAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGcc                              <  1:4444047/150‑1 (MQ=255)
                                                                                                                           gAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGtt                           >  2:1058515/1‑150 (MQ=255)
                                                                                                                           gAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGtt                           >  2:2124542/1‑150 (MQ=255)
                                                                                                                            aaaaTTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTg                          >  2:6549339/1‑150 (MQ=255)
                                                                                                                               aTTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCg                       <  1:5368068/150‑1 (MQ=255)
                                                                                                                                ttttATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTTTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGAGCCGGTGTCGc                      >  1:5915235/1‑150 (MQ=255)
                                                                                                                                 tttATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCt                     <  2:113539/150‑1 (MQ=255)
                                                                                                                                   tATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGTTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTaa                   >  2:2660463/1‑150 (MQ=255)
                                                                                                                                      ccAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCCGAGGGGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGc                >  1:6073230/1‑150 (MQ=255)
                                                                                                                                        aaaGCCAGTCCGCTGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCGGTAACGCTGGGTAATATTGCGCCGTTGTCGCTAAGGCCg              >  1:2328336/1‑150 (MQ=255)
                                                                                                                                        aaaGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCg              >  1:4767721/1‑150 (MQ=255)
                                                                                                                                        aaaGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCg              >  2:531886/1‑150 (MQ=255)
                                                                                                                                        aaaGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCg              >  2:1471258/1‑150 (MQ=255)
                                                                                                                                         aaGCCCGTCCGCTGGACTGGCTTTTGCGGTTGTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCCGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTCATATTGCGCCGGTGTCGCTAAGGCCGt             >  2:1267616/1‑150 (MQ=255)
                                                                                                                                         aaGCCAGTCCGCTGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGt             >  1:5160186/1‑150 (MQ=255)
                                                                                                                                           gCCAGTCCGCTGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCCGAGGTGGGGCAGCGAATACCTGTAACGCTGGGTCATATTGCGCCGTTGTCGCTACGGCCGTTc           >  2:672208/1‑150 (MQ=255)
                                                                                                                                           gCCAGTCCGCTGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCGGTAACGCTTGGTAAGATTGCGCCGTTGTCGCTAAGGCCGTTc           >  2:5178740/1‑150 (MQ=255)
                                                                                                                                           gCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTATTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGACTAACTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGGTc           >  1:2515520/1‑150 (MQ=255)
                                                                                                                                           gCCAGTCCAGCGGACTGGCTTTTGCGGGTTTAGCGAATAATAAATTGCGTTTCTTTGTCATTCTTTCACAACAAAAACAGGAGGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTc           >  1:1531375/1‑150 (MQ=255)
                                                                                                                                              aGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAg        >  1:6810594/1‑150 (MQ=255)
                                                                                                                                               gTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAgt       >  1:3366082/1‑149 (MQ=255)
                                                                                                                                               gTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAGc       <  1:2912432/150‑1 (MQ=255)
                                                                                                                                               gTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAGc       <  1:5512183/150‑1 (MQ=255)
                                                                                                                                                   aGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAGCCTgg   >  2:5932963/1‑150 (MQ=255)
                                                                                                                                                   aGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAGCCTgg   >  1:144703/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGGTTTGGTGGTCTGGCCGGTACCGCTGCAGGCGCAGCTAAAATTGTCTTTGTCGTCGGGATTATTCTGTTCCTGGTGAGTTTGTTCATGGGCCGAAAACGACCCTAGATTTCAGACCATTAAGAAAATTTTATCCAAAGCCAGTCCAGCGGACTGGCTTTTGCGGTTTTAGCGAATAATAAATTGCGTTACTTTGTCATTCTTTCACAACAAAAACAGGAAGGCAGAGGTGGGGCAGCGAATACCTGTAACGCTTGGTAATATTGCGCCGTTGTCGCTAAGGCCGTTCCAGCCTGGA  >  NC_000913/4612182‑4612479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: