Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 756,150 C→T 100% Q82* (CAG→TAG)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,1500CT98.5% 1920.5 / 8.4 513Q82* (CAG→TAG) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base C (5/3);  new base T (221/283);  total (227/286)
Fisher's exact test for biased strand distribution p-value = 3.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 513 total aligned reads displayed.

TGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAG  >  NC_000913/756003‑756296
                                                                                                                                                   |                                                                                                                                                  
tgtgCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAg                                                                                                                                                  >  2:215403/1‑150 (MQ=255)
 gtgCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAgg                                                                                                                                                 <  2:6066628/150‑1 (MQ=255)
  tgCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGa                                                                                                                                                <  2:725586/150‑1 (MQ=255)
    cgcTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGAcg                                                                                                                                              >  1:6434917/1‑150 (MQ=255)
     gctgctCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGAcgc                                                                                                                                             <  2:3502948/150‑1 (MQ=255)
       tgctCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGa                                                                                                                                           >  1:6565154/1‑150 (MQ=255)
       tgctCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGa                                                                                                                                           <  1:1886163/150‑1 (MQ=255)
         ctctctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGAtt                                                                                                                                         >  1:162880/1‑150 (MQ=255)
         ctctctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGAtt                                                                                                                                         >  1:2596263/1‑150 (MQ=255)
          tctctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTg                                                                                                                                        >  2:371703/1‑150 (MQ=255)
            tctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGaa                                                                                                                                      >  1:584980/1‑150 (MQ=255)
            tctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGaa                                                                                                                                      >  2:4916240/1‑150 (MQ=255)
            tctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGaa                                                                                                                                      >  1:3245051/1‑150 (MQ=255)
            tctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCAAGAAGATAACTGGCAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGAGACTAGGACGCGATTGaa                                                                                                                                      >  2:5519551/1‑150 (MQ=255)
            tctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGGTGCGCTGGGTAATACCCATGAAGATAACGGGGACTGGCATATGTACGACACCGTGACAGGGTCGGACTATATCGGTGACGAGGACGCGAGTGaa                                                                                                                                      >  2:4454770/1‑150 (MQ=255)
                aaGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAatata                                                                                                                                  <  2:5787724/150‑1 (MQ=255)
                aaGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAatata                                                                                                                                  <  1:2106197/150‑1 (MQ=255)
                 aGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAATGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGTACGCGATTGAatatat                                                                                                                                 <  2:6867285/150‑1 (MQ=255)
                 aGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAatatat                                                                                                                                 <  2:4135877/150‑1 (MQ=255)
                   tCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATAtgt                                                                                                                               <  2:6305999/150‑1 (MQ=255)
                     ttCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATgtgt                                                                                                                             >  1:3086780/1‑150 (MQ=255)
                      tCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTa                                                                                                                            >  2:1972087/1‑150 (MQ=255)
                      tCCCGACCCGTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTaa                                                                                                                           <  2:1425922/150‑1 (MQ=255)
                            cccGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCg                                                                                                                      <  1:1342432/150‑1 (MQ=255)
                             ccGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCgg                                                                                                                     <  1:1706510/150‑1 (MQ=255)
                              cGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCggg                                                                                                                    <  1:2884748/150‑1 (MQ=255)
                              cGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCggg                                                                                                                    <  1:379489/150‑1 (MQ=255)
                              cGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCggg                                                                                                                    <  1:2529648/150‑1 (MQ=255)
                                   ccATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGa                                                                                                               >  1:4056246/1‑150 (MQ=255)
                                   ccATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGa                                                                                                               >  1:3964225/1‑150 (MQ=255)
                                    cATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGaa                                                                                                              >  2:1020862/1‑150 (MQ=255)
                                    cATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGaa                                                                                                              >  1:6130324/1‑150 (MQ=255)
                                     aTACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAg                                                                                                             <  2:5898571/150‑1 (MQ=255)
                                      tACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGc                                                                                                            >  1:4458541/1‑150 (MQ=255)
                                       aCCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCg                                                                                                           >  1:1229315/1‑150 (MQ=255)
                                          gTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCTTGAATATAACTGGGATTGGAATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATTTGTTTAAAACCGGGCCGGAAGCGAtt                                                                                                        <  1:2389175/150‑1 (MQ=255)
                                            ttCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCTTGAATATAACTGGGAATGGCATATGTACGACACCGTTAATGGTTCGGACTTTTTCGTTGACTTGGACGCGTTTTAATATATGTTTAAAACGGGGCCGGAAGCGATTCt                                                                                                      <  1:6852337/150‑1 (MQ=255)
                                             tCTGCGCATGGCGGCATTACCTTTGCGTTGGTTAATACCCATGAAGATAACTGGGATTGGAATATGTACGACACCGTGAAAGGGTCGGACTATTTCGGTGACTAGGACGCGATTGAATATTTGTGTAAAACCGGGCCGGAAGCGATTCTg                                                                                                     <  1:4136484/150‑1 (MQ=255)
                                             tCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTg                                                                                                     <  2:6467775/150‑1 (MQ=255)
                                                 cgcAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAAc                                                                                                 >  1:1002753/1‑150 (MQ=255)
                                                 cgcAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAAc                                                                                                 >  2:854321/1‑150 (MQ=255)
                                                   cAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTc                                                                                               >  2:5070384/1‑150 (MQ=255)
                                                    aaGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCg                                                                                              >  1:1548552/1‑150 (MQ=255)
                                                      ggcggcATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGaa                                                                                            <  2:3283180/150‑1 (MQ=255)
                                                      ggcggcATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGaa                                                                                            <  2:5744599/150‑1 (MQ=255)
                                                      ggcggaATTACCGTTGCGCTGGTTAATACCCATTAATATAACTGGGATTGGCATATGTACGACACCTTTAATGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATTTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGaa                                                                                            <  1:2037085/150‑1 (MQ=255)
                                                       gcggcATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAac                                                                                           >  2:6683900/1‑150 (MQ=255)
                                                        cggcATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAaca                                                                                          <  2:5374892/150‑1 (MQ=255)
                                                             ttACCGGGGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATCTGTCCGACACGGTGAATGGGTCGGACTATATCGGTGACTCGGACGCGATTGAATATAGGTGTAAAACGGGGCCGGAAGCGATTCTGGAACTCGAACACATgg                                                                                     <  1:1459216/150‑1 (MQ=255)
                                                                   ttGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGc                                                                               >  1:3741430/1‑150 (MQ=255)
                                                                    tGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGcc                                                                              >  1:5493131/1‑150 (MQ=255)
                                                                      cgcTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGt                                                                            <  2:5196026/150‑1 (MQ=255)
                                                                       gcTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGtt                                                                           >  1:6209136/1‑150 (MQ=255)
                                                                        cTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTtc                                                                          <  2:2652602/150‑1 (MQ=255)
                                                                         tGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTtct                                                                         >  2:1349278/1‑150 (MQ=255)
                                                                             tAAGACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTcgcg                                                                     <  2:4829696/150‑1 (MQ=255)
                                                                                tACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGtct                                                                  >  2:623396/1‑150 (MQ=255)
                                                                                 aCCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGtctc                                                                 <  2:6501692/150‑1 (MQ=255)
                                                                                 aCCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGtctc                                                                 <  1:6743172/150‑1 (MQ=255)
                                                                                   ccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCga                                                               <  1:475050/150‑1 (MQ=255)
                                                                                   ccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCga                                                               >  2:3855811/1‑150 (MQ=255)
                                                                                    cATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCgat                                                              >  1:779926/1‑150 (MQ=255)
                                                                                    cATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCgat                                                              >  1:5544637/1‑150 (MQ=255)
                                                                                           aTAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTc                                                       <  1:564009/150‑1 (MQ=255)
                                                                                           aTAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTc                                                       <  2:2734954/150‑1 (MQ=255)
                                                                                           aTAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTc                                                       <  1:4861798/150‑1 (MQ=255)
                                                                                           aTAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTc                                                       <  2:1541042/150‑1 (MQ=255)
                                                                                           aTAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTc                                                       <  1:4691822/150‑1 (MQ=255)
                                                                                               cTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtat                                                   <  1:3430265/150‑1 (MQ=255)
                                                                                                tGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatc                                                  >  1:6399672/1‑150 (MQ=255)
                                                                                                tGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatc                                                  >  2:2971355/1‑150 (MQ=255)
                                                                                                  ggAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatcta                                                >  2:262671/1‑150 (MQ=255)
                                                                                                      tGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCaa                                            <  2:4276273/150‑1 (MQ=255)
                                                                                                        gCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACg                                          >  1:91683/1‑150 (MQ=255)
                                                                                                          atatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTc                                        >  2:83616/1‑150 (MQ=255)
                                                                                                          atatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTc                                        >  1:3299637/1‑150 (MQ=255)
                                                                                                              gTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGtt                                    >  2:2315537/1‑150 (MQ=255)
                                                                                                                 cGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTgg                                 <  1:5111131/150‑1 (MQ=255)
                                                                                                                  gACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGc                                >  1:3572036/1‑150 (MQ=255)
                                                                                                                  gACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGc                                <  2:4381658/150‑1 (MQ=255)
                                                                                                                  gACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGc                                >  2:2114591/1‑150 (MQ=255)
                                                                                                                  gACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGc                                >  2:3960804/1‑150 (MQ=255)
                                                                                                                      ccGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTc                            >  1:6036828/1‑150 (MQ=255)
                                                                                                                         tGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGt                         <  1:3630367/150‑1 (MQ=255)
                                                                                                                         tGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGt                         <  2:1751802/150‑1 (MQ=255)
                                                                                                                          gAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTc                        <  2:2823772/150‑1 (MQ=255)
                                                                                                                           aaaGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCg                       >  2:6794315/1‑150 (MQ=255)
                                                                                                                            aaGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGa                      <  2:7045954/150‑1 (MQ=255)
                                                                                                                             aGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGaa                     >  2:1187103/1‑150 (MQ=255)
                                                                                                                             aGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGaa                     <  1:7026214/150‑1 (MQ=255)
                                                                                                                               ggTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGaaaa                   >  1:6298709/1‑150 (MQ=255)
                                                                                                                                gTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGTGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGaaaaa                  <  1:2804309/150‑1 (MQ=255)
                                                                                                                                gTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGaaaaa                  <  2:4498459/150‑1 (MQ=255)
                                                                                                                                 tCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAAc                 >  1:6762402/1‑150 (MQ=255)
                                                                                                                                   ggACTTTATCGGTGACTAGGACGCGTTTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACtt               <  2:6153814/150‑1 (MQ=255)
                                                                                                                                     aCTATATCGGCGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcg             <  1:493919/150‑1 (MQ=255)
                                                                                                                                       tataTCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggc           <  2:2407113/150‑1 (MQ=255)
                                                                                                                                          atCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCggcggc        <  2:595317/150‑1 (MQ=255)
                                                                                                                                          atCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCggcggc        <  1:5269566/150‑1 (MQ=255)
                                                                                                                                            cGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGACGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGa      <  1:2729430/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAG  >  NC_000913/756003‑756296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: