Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 164,595 | T→G | 17.8% | intergenic (+61/‑135) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 164,595 | 0 | T | G | 17.8% | 397.6 / 48.2 | 168 | intergenic (+61/‑135) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
Reads supporting (aligned to +/- strand): ref base T (16/120); new base G (30/2); total (46/122) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.81e-19 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 180 total aligned reads displayed.
AGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGA > NC_000913/164447‑164743 | agttaTGAAAGGGCTTTATCCCAAACATGTCTGGCCGGACGACCGGTAAAATACTGCACCGACGCGAGGTACTAAAATTTATTCGTATGGTTGTTTTTCTCCCTCTCCCTGTGGTTGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTtc < 2:480800/146‑1 (MQ=255) ataTGAAAGGGCTTTATCCCAAACATGTCGGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACGAAAAAGTATTCTTAAGGTTGGTTTTCTCCCTCTCCCTGTGGTAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTtcac < 2:2887253/148‑1 (MQ=38) aTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCCCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTggaccc > 2:5700416/1‑150 (MQ=255) aTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCCCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTggaccc > 2:6265976/1‑150 (MQ=255) tGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTgcccccc > 1:5274309/1‑149 (MQ=255) aaaGGGCTTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGTAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctca < 2:5860616/150‑1 (MQ=255) aaGGGCGTTATCCCAAACATGTCTGGCGGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTTTTCGTAAGTTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGCCATCAGCGCGCACGTtcaccctcac < 2:2964667/150‑1 (MQ=255) tgggCTTTATCCCAAACATGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGAGGGACTAAAAAGTTTTCGTATGTTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTtcaccctcacc < 2:591227/149‑1 (MQ=37) cgggCTTTACCCCAAAATGGTGTGGCCGGACGACCCGGAAAATACTGCACCGACGCGCGGGAGGAAAATGTTGTCGTATGTTGGTTTTTCTCCCTCGCCGTGTGGGAGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTtcaccctcacc < 2:6237700/149‑1 (MQ=255) cGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCCCACCCaaa > 1:270325/1‑150 (MQ=255) cGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCC‑CACCCCaaa > 1:270348/1‑149 (MQ=255) ttATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTTCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCGCCCCCGaaac > 1:3343894/1‑148 (MQ=38) tCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCCGCGCGCACGTGGCCCCCCCCCCCAAcccac > 1:5689461/1‑148 (MQ=38) cccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTGCGCCCGCCCCCGAAccccca > 1:1996976/1‑147 (MQ=38) ccAAACTTGTCGGGCGGGCCGACCGGGAAAATACTGCCCCGACGCGACGTACGAAAACGTATTGGTATGTTTGGTTTCCCCCCTCTCCGTGGGGGAGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc < 2:5116368/150‑1 (MQ=255) ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGGCCCCCCACCCAAAcccacac > 1:6792010/1‑146 (MQ=37) caaaaaATTTCTGGCCGGACGACCGGCAAAATACTCCACCGACGCGACGTACTAAAATTTTTTCGTACGGTTGGTTTCCCCCCTCTCCCGGGGGTTGCGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc < 1:5833073/148‑1 (MQ=255) caaccctGTCTGGCCGGACGACCCGCCAAATCCGGCCCCGACGCGACGGACGAAACAGTTTTCGTTTGTTGGGTTTTCTCCCTCTCCCTGGGGGAGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc < 2:6931764/144‑1 (MQ=255) cAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc < 2:7189205/150‑1 (MQ=255) cAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCCCACCCAAAccccaacc > 2:1395437/1‑145 (MQ=255) tGTCTGGCCGGCCGACCCGCAAAATACCCCACCGCCGCGCCGCCCGAAAAAGTTTTCGAAGGGTGGGTTTCCTCCCTCTCCCTGTGGGTTTGGGCCGGGGTTAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:6132617/150‑1 (MQ=255) tGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTAGTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:2308009/150‑1 (MQ=255) tGTCTGGCCGGACGACCCGCCAAATACTGCACGGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:5939162/150‑1 (MQ=255) tGTCGGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACTAAAAATTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGCAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:2356929/150‑1 (MQ=255) ggccgggccggccGCCCCGCAAAATAGTGCACCGACGCGCGGGACTAAAAAGTATTGGTATGGTTGTTTTTCCCCCGCCCCGGGTGGGAGCGGGCCGGGGTGGGGGCCTCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:2562804/145‑1 (MQ=255) ggTCGGGCGGCACGACCCGGAAATTACTGCACCGACGCGACGGACGAAAAAGTTTTCGTACGTTTGTTTTTCACCCTCTCCCTGTGGGAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:5182982/149‑1 (MQ=37) tCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCCGCGCGCCCGGGGCCCCGCACCCCAACCCACAcccccccggg > 2:2169217/1‑143 (MQ=25) tCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGGGAGGGCAGCAGCGCGCACGGGGGCCCGCCCCCCAACCCACTCCCCCaccgg > 1:4188499/1‑146 (MQ=37) gcccGGACCACCCGGCAAAGACTCCCCCGACGCGCGGGCCGAAAATGTTTTCTTATGGTTGGTTTTCTCCCCCTCCCTGGGGGTGAGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgaga < 2:6771636/148‑1 (MQ=255) gccggccgACCCGGCAAATACTGCACCGACGCGCCGGACGAAAATGTTTTCGTATGGTTGGTTTTCTCCCCCCCCCTGTGGGAGAGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag < 2:5154517/150‑1 (MQ=255) gccgcccgacCCGCCAATCACTCACCCGTCGCGACGGACCAAAACTTATTTTAATGGTTGTTTTACCCCCTCCCCCGGTGGGAGCGGGCGGGGGTGTGGGCCTCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTTGAGGGagag < 1:1636483/144‑1 (MQ=255) cGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCACCCCCGAACACCCACCCCCACGgggggggg > 1:4962556/1‑150 (MQ=25) gacgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGGAAGGGTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCGCCCCCAAAACCACACCCCCAACGGCGAGGGGa > 2:1699595/1‑150 (MQ=25) acgacCGGGAAAATACTGCACCGACGCGAGGGACGAAAAATTATTCTTACGGTTGGTTTTCGCCCTCTCCCTGGGGGATAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGAc < 2:5608188/150‑1 (MQ=255) ctacCCGGAAAATACTGCCCCGACGGTAGGGACTAAAATTTTTTGTTATGGTTGTTTTGCTCCCTCTGCGTGTGGGTTTGGGCGGGGTTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGAcc < 1:4165252/148‑1 (MQ=255) gacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCGCCCGCACCCAAAACCCAGCCCCCCAGGGAGAGGGGACCg > 1:2960841/1‑150 (MQ=37) acCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCCCACCCCAACCCACACCCACAACGGCGCGGGGGccca > 2:5252051/1‑148 (MQ=37) ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGAACCACACCCGAACCCACACCCGCAAGGGAGGGGGGGcccagc > 2:2215407/1‑146 (MQ=25) ccGGCAAAGACTGCACCGACGCGACGTACGAAAAAGTATGCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATc < 2:6577497/150‑1 (MQ=255) gCAAATACTGCACCGACGCGACGTACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg < 2:7142547/150‑1 (MQ=255) gCACGGACGCGACGGACGAAAATGTTTTCTTTCGTTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAttt < 1:5409374/150‑1 (MQ=37) cACCGCCGCGACGCACGAAAACGTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGTGCGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 2:6848919/150‑1 (MQ=38) cACCGACGCGACGGACGAAAAAGTATTCTTAAGGTTGGTTTTCTCCCTCTCCCGGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 2:2443529/150‑1 (MQ=255) aCCTACGCTACGGACGAAAATGTATTCTTATGGTTGGTTTTCTCCCGCTCCCTTTGGTTGAGGGCCGGGGTTTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGTACCGATCGAGCACAAATTTTg < 1:3943243/150‑1 (MQ=255) cccccgcgccgCACTAAAAAGTATGCTTAAGGTTTTTTTACGCCCTCCCGTGGGGGGTGAGGGGGGGGTTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 1:1302730/146‑1 (MQ=255) ccGACGCTACGGACTAAAATGTTTTGGTATGGTTGGTTTTCTCCCACTCCGTGTGGGTGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 1:4847951/150‑1 (MQ=255) ccGACGCTACGCACTAAAAATTATTCGTATGGTTGGTTTTCTCCCTCTCCGTGTGGGAGCGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 1:6438290/150‑1 (MQ=255) ccGACGCGCCGGACGAAAAAGTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:3979770/150‑1 (MQ=255) ccGACGCGACGGACGAAAATGTATTCGTTAGGTTGGTTTTCTCCCTCGCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 1:7072439/150‑1 (MQ=255) ccGACGCGACGGACGAAAAAGTATTCGGACGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:4193762/150‑1 (MQ=255) cgacgTACTAAAAATTATTCGTATGGTTGTTTTTCCCCCCCTCCGTGTGGGTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGata < 1:155692/150‑1 (MQ=255) gacggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTGGGCCCCCACCCAAACCCCCTACCCCACAGGCGAGGGGGCCGATCGAGCCCAAAATTTTAGGGaaat > 2:1595694/1‑150 (MQ=21) gactAAAATTTATTGGTTTGGTTGTTTTTCTCCCTCCCCCTGTGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttc < 1:1157855/150‑1 (MQ=37) cgAAAATTTATTCGTTTGGTTGTTTTTCGCCCTCCCCGGGGGGGTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttctt < 2:2806739/150‑1 (MQ=255) aaaatGTATTCTTAAGTTTGGTTTACTCCAACTCCCTGTGGGAGTGGGCCGGGGTGCGGGAATCAGCGCGAACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttct < 2:713498/150‑1 (MQ=255) atgttttCGTTTGGTTGGTTTTCTCCCACGCCCGGGGGGTGCGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc < 1:5159772/145‑1 (MQ=255) aagaactCGTACGGTGGTATTACTCCCTCGCCGTGTGGGTGAGGGCCGTGTTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCATGGGTGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc < 1:5159745/144‑1 (MQ=255) aaGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCGGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc < 2:7149690/150‑1 (MQ=255) tttttCGTATGGTTGGTTTTCTCCCTCTCCGTGTGGGTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 2:4989802/147‑1 (MQ=255) ttATTCGTATGTTTGGTTTCCACCCTCTCCCTGTGGGATGGGGCCGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 1:5877418/149‑1 (MQ=255) gttttCGTTGGGTGGGTTTTCTCCCTCGCCCTGGGGGCGGGGGGGGGGGTGTGGGCATCCGCGCGCACGTGCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 1:925509/147‑1 (MQ=255) gTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCACCCAAAACCACACCCAACAGGGAGCGGGGGCCCAGCGAGCCCCAAAATTGAGAGATATAATATTCTGTGtt > 2:3472963/1‑150 (MQ=16) ttttCTTATGGTTGGTTTACGCCCTCTCGCTGGGGGTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTg < 1:2599987/148‑1 (MQ=255) tattgttAAGGGTGTTTTACTCCCTCCCCCTGTGGGAGAGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTg < 1:6831484/144‑1 (MQ=255) tATTCTTATGGTTGTTTGTCTCCCTCTCCCTGTGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTg < 1:6831894/150‑1 (MQ=39) ttCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa < 1:6237093/150‑1 (MQ=255) tcttatggttggttTGCTCCCTCCCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTaa < 1:6372632/144‑1 (MQ=255) tCGTATGTTTGGTTTCCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTaa < 1:5962616/150‑1 (MQ=39) gTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGGGGACCCCCCCCCAAAACCACACCCCACAGGGAGAGGGGGCCGAACGAGCACAAAAATTTTTAGATATATTATTATGTATTTTAACa > 1:6561091/1‑150 (MQ=12) ttggttggttTTCTCCCTCTCCGTGTGGGCGTGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga < 1:797472/148‑1 (MQ=255) tggttggttTTCTCCCTCTCCGTGGGGGAGGGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 1:2381360/149‑1 (MQ=255) ttggttTTCTCCCTCTCCGGGGGGGAGCGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAgaagaa < 2:1487028/150‑1 (MQ=255) ttttCTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGa < 2:1094025/150‑1 (MQ=255) ttttCCCCCCCTCCGTGTGGGTGTGGGCGGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGa < 1:3175407/150‑1 (MQ=255) ttCCCCCCCGCCGTGTGGTTGCGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGaaa < 2:319096/150‑1 (MQ=255) ccctctccctGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGGGCACCCCCCCCCGAAACCTCGCCCGCAAGGGCGGGGGGGCCGCTCGCGCCCACAGTTTGAGAGATATCTTCGTCTGGCGTGTAACAGCAGCACAGAAAAACg > 2:5346661/1‑150 (MQ=16) ccctctccctGGGGGTGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCg < 1:7031549/150‑1 (MQ=255) cccactccctGGGGGAGAGGGCCGGGGGGAGGGCATCAGCGCGCACGGTCACCCTCACCCTACCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCg < 1:1154335/150‑1 (MQ=255) cccctcccgGTGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCgg < 1:5843845/147‑1 (MQ=255) tgtgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCCCCCGCACCCAAAACCACAACCACAAGGGCGAGGGGACCCAAAGAGCACCAAATTTTTGAGATATAATATTATTTGTTGTTAAAGAAGAACACAAAAAAGGGCgtttta > 2:829920/1‑148 (MQ=255) gggcgcgggCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGcc < 1:764362/144‑1 (MQ=255) gggCGAGGGCCGGGGTGAGGGCCTCAGCGCGCCCATGCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGAGCGAGCACAACTTTTGAGAGATATCTTCTTCTGTCTAGTAACAGAAGAACAGAAAATCGGGCTTTTGCGcc < 1:2148265/150‑1 (MQ=255) gggggggggggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGc < 1:3740611/149‑1 (MQ=255) cGGGGTGAGGGCATCAGCGCGCCCGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGc < 2:3343894/150‑1 (MQ=255) gggTGAGGGCATCCGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCgg < 2:4093177/150‑1 (MQ=255) gggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCACAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGAGAACTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCgg < 1:1803394/150‑1 (MQ=255) ggTGGGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga < 2:2728911/150‑1 (MQ=255) ggTGCGGGCCTCCGCGCGCCCGTTCACCCTCACCCTAACCCTCTCCCTCACGGGAGAGGGGACCGAGCGAGCACAAATTTTGAGAGATCTCTTCTTCTGTCTTGTAACAGAAGAACAGAAACTCGGGCTTTTGCGCCTGAATATTGCGga < 2:5830948/150‑1 (MQ=255) ggTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga < 1:1699595/150‑1 (MQ=255) gAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaa < 2:5082732/150‑1 (MQ=255) gggCATCAGCGCGCACGTTCACCCACACACAAACCCTAACCCACCACGGCCAGGGGACCGATCCAGCACCAAATTTTAGAGATATAATCATATGTCTTTTAACAAAAGAAAAGAAAAAAGGGCGTTTGCGCCGGAATATTTCGGAGaaaa > 2:3461702/1‑150 (MQ=255) ggCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 1:5070034/150‑1 (MQ=255) aTCAGCGCGCACGTTCACCCAAACCCAAAACCAAAACCTCAAGGGAGAGGGGACCCATAGATCACAAAATTTGATATATATATTATTAAGTCTTTTAACAGAAGAACAGCAAAACGGGCTTTTTCGCCGGAAAAATTCGGGGAAAAAGCa > 2:4682484/1‑150 (MQ=255) gcgcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCg < 1:6634779/150‑1 (MQ=255) cgcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCgg < 2:5956506/150‑1 (MQ=255) cgcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCCTCTGTCATGTAACAGAAGAACAGAAAAACGGGCTTTTGCGCCAGAATATTGCGGAGAAAAAGCATGGCCgg < 1:4103472/150‑1 (MQ=255) gcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCggg < 1:366673/150‑1 (MQ=255) cACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGa < 2:1216054/150‑1 (MQ=255) cGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAAt < 2:4567010/150‑1 (MQ=255) gTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTACTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAG‑AAAAGCATGGCCGGGAATGa > 2:4471546/1‑150 (MQ=255) | AGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGA > NC_000913/164447‑164743 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |