Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,151,888 T→G 5.6% intergenic (+37/‑51) acpP → / → fabF acyl carrier protein (ACP)/3‑oxoacyl‑[acyl‑carrier‑protein] synthase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,151,8880TG 5.6% 1083.9 / 7.7 345intergenic (+37/‑51)acpP/fabFacyl carrier protein (ACP)/3‑oxoacyl‑[acyl‑carrier‑protein] synthase II
Reads supporting (aligned to +/- strand):  ref base T (168/149);  new base G (4/22);  total (172/173)
Fisher's exact test for biased strand distribution p-value = 1.80e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 354 total aligned reads displayed.

AGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAG  >  NC_000913/1151739‑1152036
                                                                                                                                                     |                                                                                                                                                    
aGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGtt                                                                                                                                                      <  2:2879358/150‑1 (MQ=255)
    ggTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTAt                                                                                                                                                  <  2:5723079/150‑1 (MQ=255)
     gTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGACATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATc                                                                                                                                                 >  1:4681629/1‑150 (MQ=255)
        aTGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCttt                                                                                                                                              >  2:5820798/1‑150 (MQ=255)
          ggCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTGTATCttttt                                                                                                                                            <  1:5023021/150‑1 (MQ=255)
             tctGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTc                                                                                                                                         >  1:5734236/1‑150 (MQ=255)
               tGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACAACAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTccc                                                                                                                                       >  1:2165575/1‑150 (MQ=255)
                ggAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTcaca                                                                                                                                      >  2:5880222/1‑150 (MQ=255)
                    gaagaGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAg                                                                                                                                  >  2:4990357/1‑150 (MQ=255)
                    gaagaGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAg                                                                                                                                  >  1:2843351/1‑150 (MQ=255)
                     aagaGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTGGTATCTTTTTGTCCCACTAGa                                                                                                                                 <  1:4864799/150‑1 (MQ=255)
                        agTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATc                                                                                                                              >  2:1282193/1‑150 (MQ=255)
                                aCTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCAtttttta                                                                                                                      >  1:5306652/1‑149 (MQ=255)
                                   gagaTTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTtccct                                                                                                                   >  1:5816285/1‑150 (MQ=255)
                                       ttCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTccctccct                                                                                                               <  1:3814403/150‑1 (MQ=255)
                                        tCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTg                                                                                                              <  1:3717712/150‑1 (MQ=255)
                                          cGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTgga                                                                                                            <  2:4213364/150‑1 (MQ=255)
                                          cGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTgga                                                                                                            <  2:3736071/150‑1 (MQ=255)
                                          cGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTgga                                                                                                            <  2:1598908/150‑1 (MQ=255)
                                          cGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTGATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTgga                                                                                                            <  1:2979921/150‑1 (MQ=255)
                                               gaagaaGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACa                                                                                                       >  2:7235074/1‑150 (MQ=255)
                                               gaagaaGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACa                                                                                                       >  1:429672/1‑150 (MQ=255)
                                               gaagaaGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACa                                                                                                       >  2:3691284/1‑150 (MQ=255)
                                                 agaagCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACaaa                                                                                                     <  2:2476715/150‑1 (MQ=255)
                                                  gaagCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAAc                                                                                                    <  1:5183570/150‑1 (MQ=255)
                                                  gaacCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAAc                                                                                                    <  2:4276661/150‑1 (MQ=255)
                                                    agCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACgt                                                                                                  <  2:3231036/150‑1 (MQ=255)
                                                    agCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACgt                                                                                                  <  2:106239/150‑1 (MQ=255)
                                                     gCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACgtg                                                                                                 >  1:3898102/1‑150 (MQ=255)
                                                          gaAAATCACCACAGTTCAGGCTGCCATTGATTACAGCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGTCCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTGCCCGCCCTGGAGGACCAACGTGTCTac                                                                                            <  2:1334214/150‑2 (MQ=255)
                                                           aaaaTCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAg                                                                                           >  1:6648741/1‑150 (MQ=255)
                                                           aaaaTCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAg                                                                                           >  1:1799077/1‑150 (MQ=255)
                                                           aaaaTCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAg                                                                                           >  1:1894683/1‑150 (MQ=255)
                                                           aaaaTCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAg                                                                                           >  1:3081601/1‑150 (MQ=255)
                                                           aaaaTCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAg                                                                                           >  1:6104481/1‑150 (MQ=255)
                                                           aaaaTCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGGGTCTAAg                                                                                           >  2:3063558/1‑150 (MQ=255)
                                                            aaaTCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGc                                                                                          <  1:7214687/150‑1 (MQ=255)
                                                               tCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTGTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGcgtc                                                                                       <  1:3565216/150‑1 (MQ=255)
                                                               tCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTGTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGcgtc                                                                                       <  1:1173846/150‑1 (MQ=255)
                                                                caccacCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGcgtcg                                                                                      >  2:5345539/1‑150 (MQ=255)
                                                                    accGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTGTTTTCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGGGTCTAAGCGTCGTGTa                                                                                  >  1:200842/1‑150 (MQ=255)
                                                                       gTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGtt                                                                               <  1:6302603/150‑1 (MQ=255)
                                                                       gTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGtt                                                                               <  1:3379746/150‑1 (MQ=255)
                                                                       gTTCAGGATTCCATTGATTACATCAACGGCCACCATGCGTAAGTGAACATCTCCAGGCGGTCGTTCTACCTCATGATTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCATCCCTTGATGACAAACGTTTCTAAGCGTATTGTAGtt                                                                               <  2:4612329/150‑1 (MQ=255)
                                                                          cAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTtgtg                                                                            <  1:3980186/150‑1 (MQ=255)
                                                                           aGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGa                                                                           <  1:541081/150‑1 (MQ=255)
                                                                            ggCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGAc                                                                          <  1:3409410/150‑1 (MQ=255)
                                                                              cTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCg                                                                        >  1:6812326/1‑150 (MQ=255)
                                                                              cTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCg                                                                        >  1:1760395/1‑150 (MQ=255)
                                                                                 ccATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGAc                                                                     <  1:5262490/150‑1 (MQ=255)
                                                                                       aTTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGGGTAGTTGTGACCGGCCTGGGCa                                                               >  1:2204330/1‑150 (MQ=255)
                                                                                        ttACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAt                                                              <  1:6170450/150‑1 (MQ=255)
                                                                                        ttACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAt                                                              >  2:2297181/1‑150 (MQ=255)
                                                                                        ttACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCACCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAt                                                              <  1:4913902/150‑1 (MQ=255)
                                                                                           cATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAtgtt                                                           <  2:2394230/150‑1 (MQ=255)
                                                                                           cATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGGTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAtgtt                                                           >  2:3105990/1‑150 (MQ=255)
                                                                                            aTCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAtgttg                                                          >  2:4049599/1‑150 (MQ=255)
                                                                                             tCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAtgttgt                                                         <  2:2145568/150‑1 (MQ=255)
                                                                                              cAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTCGTTGTGACCGGACTGGGCATGTTGtc                                                        >  2:6350957/1‑150 (MQ=255)
                                                                                              cAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGtc                                                        >  2:3894719/1‑150 (MQ=255)
                                                                                              cAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGGGGCTAAGCGTCGGGTAGTTGTGACCGGACGGGGCATGTTGtc                                                        >  1:2491956/1‑150 (MQ=255)
                                                                                              cAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCGTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGGGTAGTTGTGACCGGACTGGGCATGTTGtc                                                        >  2:1178002/1‑150 (MQ=255)
                                                                                               aaCGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGtct                                                       <  2:1673796/150‑1 (MQ=255)
                                                                                                aCGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGtctc                                                      >  1:1565709/1‑150 (MQ=255)
                                                                                                aCGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTACCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGTCCGGACTGGGCATGTTGtctc                                                      >  1:3266525/1‑150 (MQ=255)
                                                                                                 cGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTcc                                                     >  2:444480/1‑150 (MQ=255)
                                                                                                   gCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTg                                                   <  1:1324262/150‑1 (MQ=255)
                                                                                                   gCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTAGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCACCCAGGAGGACAAACGTGTCTAAGCGGCGGGTAGTTGTGAACGGACTGGGCATGTTTTCTCCTg                                                   >  1:1674448/1‑150 (MQ=255)
                                                                                                    ccaccaGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGGCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGt                                                  <  2:6037178/150‑1 (MQ=255)
                                                                                                         aGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCa                                             >  2:2717947/1‑150 (MQ=255)
                                                                                                          ggCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTGGTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCaa                                            <  1:3049945/150‑1 (MQ=255)
                                                                                                           gCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCGGAGTTTTATATTTTTGTCCCACTAGAATCATTTTTTACCACCCAGGAGGACAAACGTGTCTAAGCGTCGGGTTGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAAt                                           >  1:7001042/1‑150 (MQ=255)
                                                                                                             gTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTGATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATAc                                         <  1:1417180/150‑1 (MQ=255)
                                                                                                               aaGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCg                                       <  2:5154430/150‑1 (MQ=255)
                                                                                                               aaGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCg                                       <  1:2080316/150‑1 (MQ=255)
                                                                                                                 gTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTa                                     <  2:6854237/150‑1 (MQ=255)
                                                                                                                 gTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTa                                     <  2:3508735/150‑1 (MQ=255)
                                                                                                                 gTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTa                                     <  2:3569664/150‑1 (MQ=255)
                                                                                                                 gTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTGGGATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTa                                     <  1:195537/150‑1 (MQ=255)
                                                                                                                      aaaactcAGGCGGTCGAACGACCGCCTGGGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTc                                >  2:1345654/7‑150 (MQ=255)
                                                                                                                       aTCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCt                               >  2:480627/1‑150 (MQ=255)
                                                                                                                        tctcCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTa                              >  2:6723547/1‑150 (MQ=255)
                                                                                                                         ctcCAGGCGGTCGTTCGACCGCCTGAGTGGGATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTAc                             <  1:7078995/150‑1 (MQ=255)
                                                                                                                          ctcAGGCGGTCGAACGACCGCCTGGGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTAcc                            >  2:2020360/3‑150 (MQ=255)
                                                                                                                           ccAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCt                           <  2:6175022/150‑1 (MQ=255)
                                                                                                                             aGGCGGTCGAACGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTgg                         >  2:331866/1‑150 (MQ=255)
                                                                                                                              ggCGGTCGAACGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGa                        >  2:3188788/1‑150 (MQ=255)
                                                                                                                                 ggTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAg                     <  2:919537/150‑1 (MQ=255)
                                                                                                                                      ttCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTg                >  1:2387749/1‑150 (MQ=255)
                                                                                                                                        cGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCAACCTGGAAAGCTCTGCt              >  1:6928603/1‑150 (MQ=255)
                                                                                                                                         gACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCtt             <  2:6648741/150‑1 (MQ=255)
                                                                                                                                         gACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCtt             >  1:1021098/1‑150 (MQ=255)
                                                                                                                                         gACCGCCTGAGTGGGATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTAGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCtt             <  1:603720/150‑1 (MQ=255)
                                                                                                                                           ccGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGc           <  1:367089/150‑1 (MQ=255)
                                                                                                                                           ccGCCTGAGTGGGAGCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGc           <  1:3162597/150‑1 (MQ=255)
                                                                                                                                             gCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCg         >  1:2696199/1‑150 (MQ=255)
                                                                                                                                             gCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCg         >  2:855978/1‑150 (MQ=255)
                                                                                                                                                    ttttATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCagag  >  1:1521296/1‑150 (MQ=255)
                                                                                                                                                    ttttATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCagag  >  1:4428030/1‑150 (MQ=255)
                                                                                                                                                    ttttATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCagag  >  1:5552169/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AGCTGGTAATGGCTCTGGAAGAAGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAG  >  NC_000913/1151739‑1152036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: