Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,287,149 G→A 6.1% noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,287,1490GA 6.1% 739.2 / 28.8 268noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR
tpr
rtT sRNA, processed from tyrT transcript
protamine‑like protein
Reads supporting (aligned to +/- strand):  ref base G (146/102);  new base A (9/7);  total (158/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 276 total aligned reads displayed.

CTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTC  >  NC_000913/1287000‑1287298
                                                                                                                                                     |                                                                                                                                                      
cgtCCCCTCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCCGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCg                                                                                                                                                        <  1:1350271/148‑1 (MQ=255)
 ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCTCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCCGCAACGCGCGCTCGCTGGCGCCCGa                                                                                                                                                       >  2:5125470/1‑150 (MQ=14)
  tCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTAGTTGCCTGCGGCAACGCGCTCTCGCTGGCGCTCGac                                                                                                                                                      >  2:2174591/1‑149 (MQ=255)
    cccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGTGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTc                                                                                                                                                    <  1:1315462/150‑1 (MQ=255)
    cccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCCGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTc                                                                                                                                                    <  1:635180/150‑1 (MQ=255)
     ccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCg                                                                                                                                                   >  1:4715990/1‑150 (MQ=255)
     ccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCACGAGTCg                                                                                                                                                   >  1:3634549/1‑150 (MQ=255)
     ccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGATTCCTGCCCCAACGCGCTCTCGCTGGCGCTCCACTCg                                                                                                                                                   >  1:4216398/1‑150 (MQ=14)
     ccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGAGCTGCGAGCTTCGCCCTTCGGGTCGTTTCAGGAGGCAACGCACTCTCGCGAGCCCACGAGTCg                                                                                                                                                   >  1:407666/1‑150 (MQ=14)
       gCATGGGCAGAATAATTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAAAGCTCTCTCGCAGGCGCACGAGTCGaa                                                                                                                                                 >  1:4637785/1‑150 (MQ=14)
           gggCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAAccgg                                                                                                                                             >  2:3229992/1‑148 (MQ=255)
               aGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAATCATCGCTGCGCGCATCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGACTCGAACCTTGGTc                                                                                                                                         >  2:1372038/1‑150 (MQ=14)
                gAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCg                                                                                                                                        >  2:4993781/1‑150 (MQ=255)
                  atatTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGaa                                                                                                                                      <  2:749030/150‑1 (MQ=255)
                  atatTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGaa                                                                                                                                      <  2:1698184/150‑1 (MQ=255)
                     tttGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCt                                                                                                                                   >  2:4748123/1‑150 (MQ=255)
                        gATTGCGGATTCGCTTGAGAGTTCCGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTtct                                                                                                                                <  1:3344854/150‑1 (MQ=255)
                          ttGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGTATCTTCTCa                                                                                                                              >  2:4842562/1‑150 (MQ=255)
                          ttGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCa                                                                                                                              >  2:3001634/1‑150 (MQ=255)
                          ttGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCa                                                                                                                              <  2:2059867/150‑1 (MQ=255)
                          ttGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGACGTACCATGGTCGAAGCTGCTCa                                                                                                                              >  2:7201504/1‑150 (MQ=255)
                             cGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGATCTCTCGCTGGCGATCGAGTGGAACCTTGGTCGCAGCGTCTCATcc                                                                                                                           >  2:2572156/1‑150 (MQ=14)
                                   ggCTTGAGAGTTCCGGGTCTTTTGACAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTcccc                                                                                                                     <  1:4715158/149‑1 (MQ=255)
                                    gCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCg                                                                                                                    >  2:554233/1‑150 (MQ=255)
                                      ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTAATACTTACCCGCa                                                                                                                  >  1:452453/1‑150 (MQ=255)
                                      ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCCGCAACGCTCTCTCGCAGGCGCGCGAGTCGAACCGTGGTCGAAGCTTCTAATCCTTCCCCGcc                                                                                                                  >  2:6856379/1‑149 (MQ=14)
                                      ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAAACGTGGTCGAAGCGTCACATCCTTCCCCGcc                                                                                                                  >  1:5796740/1‑149 (MQ=14)
                                       tGAGAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCAt                                                                                                                <  1:5316831/150‑1 (MQ=255)
                                         agagTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATgg                                                                                                              >  2:4769622/1‑150 (MQ=255)
                                         agagTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCACCGCTGCGCGCTTCGCCCATCGGGTCGTTTCCGCCCGCAACGCACTCTCGCTCCCCCACCAGTCGGAACTTGGAAGAAGCTTATCATAATTCCCCCCATgg                                                                                                              >  2:6148348/1‑150 (MQ=9)
                                            gTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGCATgggaa                                                                                                           >  2:5365685/1‑148 (MQ=255)
                                             ttCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCATCGGCAACCCACTCAAGCTGGCCCACGAGTAGAACCGTGGTCGAAGCTTCTAATCCATCCCCGCAGGGGCAg                                                                                                          >  1:1375688/1‑150 (MQ=14)
                                               cAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGaa                                                                                                        <  1:5032727/150‑1 (MQ=38)
                                                   gccTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGACGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAtt                                                                                                    <  1:2803264/148‑1 (MQ=21)
                                                      ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGAAGTGGGCaaaaattaaa                                                                                                  >  2:6458398/1‑144 (MQ=11)
                                                        ttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCACTCAAGCAGGCGCGCGAGTCGAACCATGGTCGAAGCATATAAACAATCCCCCCCAGGGCCGAATAATTaaat                                                                                               >  1:313119/1‑148 (MQ=11)
                                                        ttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGAGCTTCGCCCTACGGGTCGATGCCTGCGGCAACGCACTCTCGCTGGCGCTAGAGGCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAAta                                                                                               >  2:4166843/1‑149 (MQ=11)
                                                                     tggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATc                                                                                                                            <  1:908196/109‑1 (MQ=32)
                                                                     tggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTgg                                                                                  <  1:217849/150‑1 (MQ=38)
                                                                     tggtggGGGAAGGATGACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTgg                                                                                  <  1:1920540/150‑1 (MQ=38)
                                                                         gggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCCCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAg                                                                              <  1:4364481/150‑1 (MQ=255)
                                                                           gggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGt                                                                                                                                          <  1:3977708/89‑1 (MQ=33)
                                                                               aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAgg                                                                        <  2:6767204/150‑1 (MQ=255)
                                                                                      cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTGGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTGGGGAAGTTCAGGGGCttgt                                                                 >  2:5990748/1‑148 (MQ=255)
                                                                                       tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTg                                                                                                                                            >  2:94886/1‑75 (MQ=34)
                                                                                       tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTg                                                                                                                                            <  1:94999/75‑1 (MQ=34)
                                                                                                cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtg                                                       <  2:705411/150‑1 (MQ=255)
                                                                                                cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtg                                                       <  1:1461185/150‑1 (MQ=255)
                                                                                                         cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAAACTTGGTAGAAGCTTCTCAACCTTCCCCGCATGGGCAGAATATTTAAATTCGGATGCGTTGGGAACGTAAGGGACTTTTGAAATTGGTGGTGGTgggg                                              >  2:3658355/1‑150 (MQ=17)
                                                                                                         cccGTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTgggg                                              <  2:4291325/150‑1 (MQ=255)
                                                                                                           cTTCGGGTCGTTGCCTGCGGCAACGCGCTCTCGCTGGCCCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGCAGGGGCACAAAATTTAAATGCGCAAACAATGTGACAGACAGGGAGGTTTGAAAGTGCTGGTGGTgggggc                                            >  2:2249420/1‑149 (MQ=9)
                                                                                                            ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                           <  1:7148816/150‑1 (MQ=255)
                                                                                                            gagagagcGTTGCCGCAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                           >  1:4481364/8‑150 (MQ=17)
                                                                                                              cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGTGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAgg                                         <  1:6746372/150‑1 (MQ=255)
                                                                                                               agagcGTTGCCGCAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCATCCCCGCATGGGCAGAATAGTTAATTGCGGATTCGTTGGGAAGTTCAGGGCCTTTTGAAAGTGATGGTGGTGGGGGACGGa                                        >  1:1241856/5‑150 (MQ=17)
                                                                                                                ggTCGTTGCCAGCGGCAACCCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCCCAAGGGCAGAATATTTAATTTCGGATTCCTTGGGAACGTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAt                                       >  2:5586826/1‑150 (MQ=17)
                                                                                                                 agcGTTGCCGCAGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAtt                                      >  2:452081/3‑150 (MQ=11)
                                                                                                                  tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGACAGTGATGGTGGTGGGGGAAGGATTc                                     >  2:3895280/1‑150 (MQ=255)
                                                                                                                  tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTc                                     <  1:4669044/150‑1 (MQ=255)
                                                                                                                     ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACt                                                                    <  1:1100722/116‑1 (MQ=255)
                                                                                                                     ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACt                                                                    >  2:1100722/1‑116 (MQ=255)
                                                                                                                      tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGATTATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAc                                 <  1:3011105/150‑1 (MQ=255)
                                                                                                                        ccTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAcca                               >  2:5785475/1‑149 (MQ=255)
                                                                                                                        ccTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAg                                                                         >  2:4064857/1‑108 (MQ=255)
                                                                                                                        ccTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCt                               >  2:3531889/1‑150 (MQ=255)
                                                                                                                         cTGCGGCAACGCTCTCTCGGTGTCGCTCGAGCCGAACCTTGGTCGTAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTGGGTAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCtt                              <  1:5598059/150‑1 (MQ=14)
                                                                                                                         cTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAt                                                                                                                             <  1:3080516/56‑1 (MQ=35)
                                                                                                                         cTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCATTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCtt                              >  2:1131912/1‑150 (MQ=255)
                                                                                                                         cTGCGGCAACGCTCTCTAGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTACCCGCATGGGCAGAATATTTAATTGCGGATTCGTTTGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAccat                              >  1:6933318/1‑148 (MQ=255)
                                                                                                                          tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                             <  2:6734343/150‑1 (MQ=255)
                                                                                                                          tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                             <  2:4697315/150‑1 (MQ=255)
                                                                                                                          tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAAGATTGAAATGCGGAGTCGGTGGGAAGTTCAGGGACTTTTGTAAGTGATGGTGGTGGGGGAGGGAGTCGAACCTTc                             >  2:5710142/1‑150 (MQ=17)
                                                                                                                           gCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCg                            <  1:4769622/150‑1 (MQ=255)
                                                                                                                               cAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGt                        <  1:251294/150‑1 (MQ=255)
                                                                                                                                 aCGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCg                      >  2:4606263/1‑150 (MQ=255)
                                                                                                                                  cGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCACATACAACCCCGCATGGGCAGAAAATTTAATTGCGGATTCGGTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGGAGGATTCGAAACATCGAACTCGa                     >  1:1040941/1‑150 (MQ=17)
                                                                                                                                   gCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGAt                    <  2:3122217/150‑1 (MQ=255)
                                                                                                                                   gCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGa                                                         <  1:3551348/113‑1 (MQ=255)
                                                                                                                                     tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGa                  >  2:2549915/1‑150 (MQ=255)
                                                                                                                                     tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATACTTCCCCCCATGGGCAGAAAATTTAATTGCGGATTCGTTGGGAAGTTAAGGGACTTTTGAAATTGATGGTGGTGGGGGACGGATTAGAAACAACGAACTCGATGa                  >  1:5540371/1‑150 (MQ=17)
                                                                                                                                     tctcacGCTGGCGCTCGAGTCGAACCATGGTCGAATCATATCAACCATACCCGCAAGGGCAGAATATTTAATTGCGGATTAGTTGGGAAGTTAAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCATCGAAGTCAATGa                  >  1:3374595/1‑150 (MQ=18)
                                                                                                                                       tctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACg                >  2:2866496/1‑150 (MQ=255)
                                                                                                                                        ctcGCTGGCGCTCGAGTCGAACCATGGTCGAAGCATCTCATCCTTCCCCGCATGGGCACAAAATTTAAATGCGGAATCGTTGGGAAGTTCAGGGACATTTTAAAGTGATGGTGGTGGGGGAAGGATTCGAACCATCGAAGACGATGAccg               >  1:5391175/1‑148 (MQ=18)
                                                                                                                                         tcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGCTTCGAACCTTCGAAGTCGATGACGGc              >  1:4400178/1‑150 (MQ=255)
                                                                                                                                         tcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATACATACCCGCAAGGGCAGAATATTTAATTGCGGATTAGTTGGGAAGTTAAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCATCGAAGTCGATGACGGc              >  1:1601260/1‑150 (MQ=18)
                                                                                                                                         tcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGCAGGGGCAGAAAATTAAAATGCGCAGACGTGGGGACGGTCAGCGACTTTTGAAAGTGATGGGGGTGGGGGAAGGATTCGAACCTGCGAAGACGAAGACGGc              >  2:5322868/1‑150 (MQ=18)
                                                                                                                                         tcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGttt                                                                                   >  1:6061012/1‑80 (MQ=17)
                                                                                                                                         tcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTg                                                                                   <  2:6061012/81‑1 (MQ=25)
                                                                                                                                         tcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTg                                                                                   <  1:2299378/81‑1 (MQ=25)
                                                                                                                                           gCTGACGCTCGAGACGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGGTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAACATCGAAGACGATGACCGCac            >  2:3046793/1‑149 (MQ=17)
                                                                                                                                                cgcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTgg                                                                                  <  2:1965538/75‑1 (MQ=255)
                                                                                                                                                 gcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAc      <  1:4748159/150‑1 (MQ=255)
                                                                                                                                                 gcTCGAGGCGAACCTTGGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAc      <  2:23136/150‑1 (MQ=255)
                                                                                                                                                   tCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAg    >  2:3400983/1‑150 (MQ=255)
                                                                                                                                                   tCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAg    >  2:7168307/1‑150 (MQ=255)
                                                                                                                                                   tCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAg    >  2:874890/1‑150 (MQ=255)
                                                                                                                                                     gAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGACGTTCAGGGACTTTTGAAAGTGATGGTGGGGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATATACAGTc  >  2:4108466/1‑150 (MQ=255)
                                                                                                                                                     gAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTc  >  1:2623287/1‑150 (MQ=255)
                                                                                                                                                     gAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTc  <  1:773302/150‑1 (MQ=255)
                                                                                                                                                     gAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAGAACCATCGAAGTCGATGACGGCAGATTTACAGTc  >  1:2001556/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTC  >  NC_000913/1287000‑1287298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: