Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,466,336 C→G 6.0% pseudogene (945/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,466,3360CG 6.0% 1008.3 / 37.8 301pseudogene (945/2513 nt)ydbApseudogene, autotransporter homolog; interrupted by IS2 and IS30
Reads supporting (aligned to +/- strand):  ref base C (103/175);  new base G (11/8);  total (115/186)
Fisher's exact test for biased strand distribution p-value = 8.83e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 320 total aligned reads displayed.

ATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGA  >  NC_000913/1466187‑1466485
                                                                                                                                                     |                                                                                                                                                     
aTTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATAGTCAAGAACGAAGGCGAGAGCACCATCACCAACGGTggcaccggcacc                                                                                                                                                       >  1:6373394/1‑150 (MQ=255)
 ttGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCAccc                                                                                                                                                      <  1:491151/150‑1 (MQ=255)
    atatCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGa                                                                                                                                                   <  1:1856807/150‑1 (MQ=255)
         aCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAAt                                                                                                                                              <  2:2779326/150‑1 (MQ=255)
          ccGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATg                                                                                                                                             <  1:7158391/150‑1 (MQ=255)
           cGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACCCAGATTAATgg                                                                                                                                            <  2:3040878/150‑1 (MQ=255)
            ggTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGt                                                                                                                                           <  1:2500964/150‑1 (MQ=255)
            ggTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGt                                                                                                                                           <  2:2041638/150‑1 (MQ=255)
            ggTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGt                                                                                                                                           <  2:5652322/150‑1 (MQ=255)
              tGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTAGACGGAGACAAGGCCATAGTAAAAAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAAAGGtga                                                                                                                                         >  1:2876087/1‑150 (MQ=11)
               gACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGtgat                                                                                                                                        <  2:5299013/150‑1 (MQ=255)
                 cAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAATGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGtgatga                                                                                                                                      <  2:4477563/150‑1 (MQ=255)
                  aGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGAc                                                                                                                                     <  1:2092162/150‑1 (MQ=255)
                   gcgcGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACg                                                                                                                                    <  1:6330880/150‑1 (MQ=255)
                       gACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCAc                                                                                                                                >  1:434063/1‑150 (MQ=255)
                       gACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCAc                                                                                                                                <  1:4226102/150‑1 (MQ=255)
                       gACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCAc                                                                                                                                <  2:1026627/150‑1 (MQ=255)
                         cGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACgg                                                                                                                              <  2:5516243/150‑1 (MQ=255)
                           gTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTaa                                                                                                                                               >  2:5406581/1‑131 (MQ=255)
                           gTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCa                                                                                                                            <  2:7083992/150‑1 (MQ=255)
                           gTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCa                                                                                                                            <  2:16588/150‑1 (MQ=255)
                              gATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAaac                                                                                                                         <  2:3383590/150‑1 (MQ=255)
                              gATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAaac                                                                                                                         <  2:696332/150‑1 (MQ=255)
                              gATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAAAGGTGATGACGCCACGGCAaac                                                                                                                         >  1:1181390/1‑150 (MQ=255)
                              gATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATAGACGGTGACAAGGCCATAGTGAATAACGAAGGCGAGAGCTCCATCACCAACGGTGGCACCGGCACGCAGATTAAAGGTGATGACGCCACGGCAaac                                                                                                                         >  1:667354/1‑150 (MQ=14)
                               aTAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGTACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAaaca                                                                                                                        >  2:5102570/1‑150 (MQ=255)
                                tAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAaacaa                                                                                                                       <  1:3758910/150‑1 (MQ=255)
                                   cAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAaacaacaa                                                                                                                    >  2:6564471/1‑150 (MQ=255)
                                    aaGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGAGACAAGGCCATAGTGAAGAACGAAGGCGAGAGCGCCATCACCAGATCTGGCGACGGCAGGCAGAGGAAAGGTTGTGACGCCACGGCAagctacagc                                                                                                                   >  2:71769/1‑148 (MQ=9)
                                     aGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACg                                                                                                                  <  2:3881228/150‑1 (MQ=255)
                                       ggCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGc                                                                                                                >  1:6301169/1‑150 (MQ=255)
                                            cATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAac                                                                                                           <  1:6925302/150‑1 (MQ=255)
                                               gACCGTCACCGATCCGGTGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAaccac                                                                                                        <  1:6237661/150‑1 (MQ=255)
                                               gACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAaccac                                                                                                        <  2:1949871/150‑1 (MQ=255)
                                                aCCGTCACCGATCCGGAGTCCTTGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAaccacc                                                                                                       <  1:4903141/150‑1 (MQ=255)
                                                aCCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAaccacc                                                                                                       <  1:570381/150‑1 (MQ=255)
                                                   gTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGtt                                                                                                    >  2:2899352/1‑150 (MQ=255)
                                                      aCCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGAc                                                                                                 <  1:4005619/150‑1 (MQ=255)
                                                      aCCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAAAGGTGATGACGCCACGGCAAACAACAACGGCAAAACAACCgagatc                                                                                                 >  2:2393251/1‑145 (MQ=255)
                                                        cGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGATGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACgg                                                                                               >  1:2707493/1‑150 (MQ=255)
                                                        cGATCCGGAGTCCATGGGTATCCAGATCGACGGAGACAAGGCCATCGTCAATAACGAAGGCGAGAGCCCCCTCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACgg                                                                                               >  2:5178338/1‑150 (MQ=255)
                                                          aTCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCa                                                                                             >  1:5051205/1‑150 (MQ=255)
                                                          aTCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCa                                                                                             >  1:3249616/1‑150 (MQ=255)
                                                           tCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCaa                                                                                            <  2:4612159/150‑1 (MQ=255)
                                                           tCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCaa                                                                                            >  1:827864/1‑150 (MQ=255)
                                                            ccGGCGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGATGGCGAGATCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGAAAACAACAACGGCAAAACCACCGTTGACGGCAAg                                                                                           <  1:3430432/150‑1 (MQ=255)
                                                            ccGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAg                                                                                           <  2:528930/150‑1 (MQ=255)
                                                             cGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAAAGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAgg                                                                                          >  2:1715612/1‑150 (MQ=255)
                                                              ggcgTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGa                                                                                         >  1:1158571/4‑150 (MQ=255)
                                                              ggAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAAGGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGa                                                                                         >  2:2204015/1‑150 (MQ=255)
                                                              ggAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGa                                                                                         >  2:6736497/1‑150 (MQ=255)
                                                               gAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGAGTAATGGTGATGACGCCACGGCACACAACACCGGCAAAACCACCGGTGCCGGCAAGGag                                                                                        >  2:4299560/1‑149 (MQ=18)
                                                                aGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATACCGAAGGCGAGAGCACCATCACCAACGGGGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCTAGGAtt                                                                                       <  2:1307454/150‑1 (MQ=255)
                                                                   ccATGGGTATCCAGATCTACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGATCACCATCACCAACGGTGGCACCGGCAACCATATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCa                                                                                    <  1:6017419/150‑1 (MQ=255)
                                                                    cATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAc                                                                                   >  1:6711956/1‑150 (MQ=255)
                                                                    cATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAc                                                                                   <  1:4078097/150‑1 (MQ=255)
                                                                     aTGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAcc                                                                                  >  1:5402563/1‑150 (MQ=255)
                                                                      tGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCg                                                                                 <  1:5450395/150‑1 (MQ=255)
                                                                      tGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCg                                                                                 <  2:6213998/150‑1 (MQ=255)
                                                                         gTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTa                                                                              >  1:2288509/1‑150 (MQ=255)
                                                                              cAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCaa                                                                                                                           <  2:2549645/100‑1 (MQ=255)
                                                                              cAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGaa                                                                         >  1:2554620/1‑150 (MQ=255)
                                                                               aGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGaaa                                                                        >  1:4759998/1‑150 (MQ=255)
                                                                                    gACGGTGACAAGGCCATCGTCTATCACGTAGGCGAGAGCTCCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAAc                                                                   <  1:1421869/150‑1 (MQ=255)
                                                                                     aCGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACg                                                                  <  2:6144472/150‑1 (MQ=255)
                                                                                           aCAAGGCCATCGTCAATAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGtgatga                                                                                                                                      >  1:200581/1‑76 (MQ=14)
                                                                                               tgcgttCGTCTATCACGTAGGCTAGAGCACCATATCCAACGGTGGCCGCGGGACCGAGATTAATGGTGATGACGCCACTGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACgg                                                        <  1:356173/145‑1 (MQ=17)
                                                                                               ggCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAAGGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAAttg                                                                     >  2:1347709/1‑135 (MQ=255)
                                                                                               ggCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAAGGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAAttg                                                                     <  1:1347709/137‑3 (MQ=255)
                                                                                                gCCCTCGTCTATCACGAAGGCGAGAGCTCCATCACCAACGGTGGCCCCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGAAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGa                                                       <  1:5524926/150‑1 (MQ=17)
                                                                                                gCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCAACGGCAAACAAAAACGTCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGACATCAACGGTAATAACGGa                                                       >  1:1085556/1‑150 (MQ=255)
                                                                                                         aaTTACGAAGGCGAGAGCACCATCACCAACGGTGGCCCCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTAtt                                              <  1:6981773/150‑1 (MQ=255)
                                                                                                         aaTAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTAtt                                              <  1:5157529/150‑1 (MQ=255)
                                                                                                         aaTAACGAAGGCGAGACCACCAACACCAACGGTGGCACCGGCACCCAGATAAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTAtt                                              <  1:1968993/150‑1 (MQ=255)
                                                                                                           tAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCa                                            >  2:6413704/1‑150 (MQ=255)
                                                                                                           tAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCa                                            <  2:3162101/150‑1 (MQ=255)
                                                                                                                aaGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACg                                       <  1:941622/150‑1 (MQ=255)
                                                                                                                  ggCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGc                                     <  1:1717452/150‑1 (MQ=255)
                                                                                                                   gCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAGAGTTATTCAGGACGGCg                                    >  2:1788849/1‑150 (MQ=255)
                                                                                                                   gCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAACGGTGATGACGCCACGGCaaa                                                                                                                          <  2:5040128/64‑1 (MQ=255)
                                                                                                                     gagaGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGAt                                  <  1:3019895/150‑1 (MQ=255)
                                                                                                                     gagaGCAACATCACCAACGGTGGCACCGGCACAAAGATTAAATGTTAAGACTCCAAGGCAAACAACAACGTCAAAACCACCGTAGACGGCAAGGATAACACAGTTACGGAAATCAACGTTAATAACGGAAAAGTTATACAGGACGGCTAt                                  >  1:1033250/1‑150 (MQ=17)
                                                                                                                         gCACCAACACCAACGGTGGCTCCGTCACCGAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTgg                              <  1:4240827/150‑1 (MQ=255)
                                                                                                                          ctcgctCACCAACGGGGGCCCCGTGACCGATATTAAGGGTGATTACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGa                             <  2:1156103/145‑1 (MQ=17)
                                                                                                                          cACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGa                             <  1:6099380/150‑1 (MQ=255)
                                                                                                                           agcttCACCCACGGTTGGATCGGCCGACATATTACGGGTGATGACGCCACTGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGAt                            <  2:6126237/146‑1 (MQ=14)
                                                                                                                           aCCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGAt                            >  2:1529635/1‑150 (MQ=255)
                                                                                                                           aCCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGAt                            <  2:2316191/150‑1 (MQ=255)
                                                                                                                           aCCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGAt                            <  1:6148857/150‑1 (MQ=255)
                                                                                                                            ccATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGGTGACGGCACGGATTCCACCGGTACGGAAATCCCCGGGAATCACGGAACAGTGATGCAGGCCGGCGATCTGGCTg                           >  2:6713316/1‑150 (MQ=14)
                                                                                                                               tCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCa                        <  2:2707493/150‑1 (MQ=255)
                                                                                                                                  ccAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCAc                                                                                                                                >  1:2142986/1‑43 (MQ=255)
                                                                                                                                   cAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgcgg                    >  1:1006612/1‑150 (MQ=255)
                                                                                                                                    aaCGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAaacaacaac                                                                                                                   >  1:2447662/1‑54 (MQ=255)
                                                                                                                                     ccGGGGGCACCGGCACCCAGACGCCTGGAGAGGACGCCACGGCAAACAACAACGGCAACCCCACCGTGGACTGCTAGGAGTCGACCGGTACGGAAACCAACGGGAAGCCCGGAAATGTCTTTCAGGACGGCGATCTGGATGTCAgcggcg                  <  2:4377643/149‑1 (MQ=9)
                                                                                                                                      cGGTGGCACCGCCACCCAGATTAATGGTGATTACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAgcggcgg                 <  2:1882852/150‑1 (MQ=255)
                                                                                                                                              ccggccccCAGATTAAGGGTGATGACGCCACGGCAAACAACAACGGCAAACCCACCGTTGACGGCAAGGATTCCACCGGGACGGACATCAACGGTAATACCGGAAAAGTTATTCCGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACg         <  1:4685676/150‑1 (MQ=17)
                                                                                                                                               cggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCaa                                                                                            <  2:4019204/66‑1 (MQ=40)
                                                                                                                                                  caccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAATGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTAt     >  1:3191062/1‑150 (MQ=255)
                                                                                                                                                     ccAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGa  <  1:1529635/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATCAACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGA  >  NC_000913/1466187‑1466485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: