Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,885,422 A→T 6.4% E253V (GAA→GTA)  yeaW → putative YeaWX dioxygenase alpha subunit; 2Fe‑2S cluster

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,885,4220AT 6.4% 1123.4 / 8.4 385E253V (GAA→GTA) yeaWputative YeaWX dioxygenase alpha subunit; 2Fe‑2S cluster
Reads supporting (aligned to +/- strand):  ref base A (178/175);  new base T (27/3);  total (207/178)
Fisher's exact test for biased strand distribution p-value = 1.38e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 389 total aligned reads displayed.

CGATAACTATCTCGAGTGCTATCACTGTGGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCA  >  NC_000913/1885273‑1885571
                                                                                                                                                     |                                                                                                                                                     
agATAACTATCTCGAGTGCTATCACTGTGGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGa                                                                                                                                                       <  2:3937747/149‑1 (MQ=255)
  aTAACTATCTCGAGTGCTATCACTGTGGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAag                                                                                                                                                     >  1:1103008/1‑150 (MQ=255)
  aTAACTATCTCGAGTGCTATCACTGTGGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAag                                                                                                                                                     >  1:53470/1‑150 (MQ=255)
    aaCTATCTCGAGTGCTATCACTGTGGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAagag                                                                                                                                                   <  1:3849871/150‑1 (MQ=255)
     aCTATCTCGAGTGCTATCACTGTGGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAgg                                                                                                                                                  <  2:834156/150‑1 (MQ=255)
      cTATCTCGAGTGCTATCACTGTGGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAggg                                                                                                                                                 >  2:6951391/1‑150 (MQ=255)
       tATCTCGAGTGCTATCACTGTGGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGt                                                                                                                                                >  1:3509010/1‑150 (MQ=255)
       tATCTCGAGTGCTATCACTGTGGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTGTAAATTTGAAGAGGGt                                                                                                                                                >  2:2743966/1‑150 (MQ=255)
           tcGAGTGCTATCACTGTGGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTTAAGAGGGTACgg                                                                                                                                            >  1:3636441/1‑150 (MQ=255)
             gAGTGCTATCACTGTGGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTTAAGAGGGTACGGAt                                                                                                                                          >  1:6362502/1‑150 (MQ=255)
              aGTGCTATCACTGTGGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTTAAGAGGGTACGGATg                                                                                                                                         >  1:2473918/1‑150 (MQ=255)
                tGCTATCACTGTGGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCg                                                                                                                                       <  1:5771399/150‑1 (MQ=255)
                 gCTATCACTGTGGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCgg                                                                                                                                      <  2:6868382/150‑1 (MQ=255)
                   tATCACTGTGGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCa                                                                                                                                    >  2:3274112/1‑150 (MQ=255)
                   tATCACTGTGGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCa                                                                                                                                    >  1:1528059/1‑150 (MQ=255)
                   tATCACTGTGGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCa                                                                                                                                    >  1:5420294/1‑150 (MQ=255)
                   tATCACTGTGGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCa                                                                                                                                    >  1:2847203/1‑150 (MQ=255)
                   tATCACTGTGGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGGACGGATGCGGCa                                                                                                                                    >  2:2601290/1‑150 (MQ=255)
                      cACTGTGGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTc                                                                                                                                 <  1:3087590/150‑1 (MQ=255)
                      cACTGTGGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTc                                                                                                                                 <  1:3566812/150‑1 (MQ=255)
                        cTGTGGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCa                                                                                                                               <  1:5292973/150‑1 (MQ=255)
                        cTGTGGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCa                                                                                                                               <  2:4855523/150‑1 (MQ=255)
                         tgtgGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCAc                                                                                                                              >  1:4649676/1‑150 (MQ=255)
                          gtgGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACg                                                                                                                             <  2:5517464/150‑1 (MQ=255)
                                cGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCt                                                                                                                       <  1:1354959/150‑1 (MQ=255)
                                 ggCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTg                                                                                                                      <  2:2568902/150‑1 (MQ=255)
                                 ggCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGGTTCTg                                                                                                                      >  2:6228221/1‑150 (MQ=255)
                                  gcgcATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTgg                                                                                                                     >  2:1681894/1‑150 (MQ=255)
                                  gcgcATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTGTAAATTTGAAGAGGGTACGGATGCGGCCTTCCACGGTTTCTgg                                                                                                                     >  1:1006597/1‑150 (MQ=255)
                                        ccAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTgg                                                                                                               <  1:4038707/150‑1 (MQ=255)
                                        ccAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGGCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCCCCGTTTCTGGCtgttg                                                                                                               >  2:1328325/1‑148 (MQ=255)
                                          aGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGcc                                                                                                             >  2:4409631/1‑150 (MQ=255)
                                            gTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGt                                                                                                           <  2:1492331/150‑1 (MQ=255)
                                            gTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGt                                                                                                           <  1:1885343/150‑1 (MQ=255)
                                                  ccGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTTTATTGGGTTAGGATGCGGCATTACACGGTTTATTGGTGTTGCCGTGGACGa                                                                                                     >  1:2689559/1‑150 (MQ=255)
                                                       tCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTTAAGAGGGTACGGATGCGGCATTCCACGGTTACTGGCTGTGGCCGTGCACGATGcag                                                                                                >  1:6761101/1‑148 (MQ=255)
                                                         cGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGaa                                                                                              <  1:5827660/150‑1 (MQ=255)
                                                         cGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGaa                                                                                              <  2:6378604/150‑1 (MQ=255)
                                                         cGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGaa                                                                                              <  1:6286225/150‑1 (MQ=255)
                                                         cGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGaa                                                                                              <  2:1230973/150‑1 (MQ=255)
                                                         cGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGaa                                                                                              <  2:190140/150‑1 (MQ=255)
                                                            aCAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGt                                                                                           >  2:3618534/1‑150 (MQ=255)
                                                                gTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTGTAAATTTGAAGATGGTACGGATGCGGCATAACAACGTTTATGGCTGTGGCCGTGCACCATTCTGAACCTCAcc                                                                                       >  1:1252241/1‑150 (MQ=255)
                                                                 ttGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTGTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCGGTGGCCCTGCACGATGCTGTACGTCAccc                                                                                      >  2:3356122/1‑150 (MQ=255)
                                                                    aTCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGa                                                                                   <  2:2191027/150‑1 (MQ=255)
                                                                    aTCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGa                                                                                   <  1:3335875/150‑1 (MQ=255)
                                                                     tCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGAt                                                                                  <  1:5521320/150‑1 (MQ=255)
                                                                     tCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGAt                                                                                  >  2:6568400/1‑150 (MQ=255)
                                                                      cGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCAATCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATc                                                                                 >  1:3496050/1‑150 (MQ=255)
                                                                       gTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCCCGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCa                                                                                >  1:4459262/1‑150 (MQ=255)
                                                                       gTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCa                                                                                >  2:2259074/1‑150 (MQ=255)
                                                                        ttACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTTAATAGGGTACGGATGCGGCATTCCACGGTTTCTGGCGGTGGCCGTGCACGATGCTGAACGTCACCCCGATCaa                                                                               >  1:6466785/1‑150 (MQ=255)
                                                                        ttACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAAACTCACCCCGATCaa                                                                               >  2:6164205/1‑150 (MQ=255)
                                                                         tACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTATATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCaaa                                                                              <  2:5466177/150‑1 (MQ=255)
                                                                         tACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAAATTTAAGAGGGTACGGATGCGGCAATCCACCGTTTATTGCTGTTGCCGTGCACGATGCTGAACGTCACCCCCATCaaa                                                                              >  1:5655853/1‑150 (MQ=255)
                                                                            tGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGTAGAGGGTACGGATGCGGCCATACACGCTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGAACAAAggg                                                                           >  1:6932027/1‑150 (MQ=255)
                                                                            tGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCCCGGTTTCTGGCTGTGGCCGTGCACGCTGCTGAACGTCACCCCGATAAAAggg                                                                           >  1:6204793/1‑150 (MQ=255)
                                                                              gCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTTAATTTGAAGAGGGTACGGATGCGGCATTACACGGGTTATGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCCAAGGgat                                                                         >  1:6982443/1‑150 (MQ=255)
                                                                              gCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTTTAGTGGGTACGGATGCGGCATTACACGGTTTCTGGCTGTGGCCGTGCACGTTGCTGTACGTCACCCCGATGAATGGgat                                                                         >  2:5931450/1‑150 (MQ=255)
                                                                              gCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTTAAGAGGGTAAGGATGCGGCCATCCACGGTTTCTGGCTGTGGCCGTGCACGAAGCAGAACGTCACCCCCATCAAAGGgat                                                                         >  1:2999572/1‑150 (MQ=255)
                                                                              gCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGgat                                                                         >  2:4039269/1‑150 (MQ=255)
                                                                               cacaCCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTTAAGAGGGTACGGCTGCGGCATTCCACGGTTTATGGCTGTGGCCGTGCACGATGCTGAAAGTCACCCCCATCAAAGGgatg                                                                        >  1:891741/1‑150 (MQ=255)
                                                                               cacaCCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGgatg                                                                        <  2:5183509/150‑1 (MQ=255)
                                                                                acacCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTATTGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGgatga                                                                       >  2:6801420/1‑150 (MQ=255)
                                                                                   ccATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTTAAGAGGGTACGGATGCGGCATTCCACGGTTTATGGCTGTGGGCGTGCACGATGCTGTACGGCACCCCGATCAAAGGGAtgatga                                                                    >  1:7173112/1‑150 (MQ=255)
                                                                                    cATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGAc                                                                   <  2:53470/150‑1 (MQ=255)
                                                                                      tGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACgg                                                                 <  1:4173805/150‑1 (MQ=255)
                                                                                       gCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGt                                                                <  2:2314324/150‑1 (MQ=255)
                                                                                        cACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAAATTGAAGAGGGTACGGATGCGGCATTCCACCGGTTCTGGCTGTGGCCGGGCCCGATGCTGAACGTCCCCCCCATCAAAGGGCTGATGACGGTc                                                               >  1:4813395/1‑150 (MQ=255)
                                                                                         aCGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGGTTCTGGCTGTGGCCGTGCACGATGCGGTACGTCACCCCGATCAAAGGGAGGATGACGGTCa                                                              >  2:4468387/1‑150 (MQ=255)
                                                                                             tAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTa                                                          <  1:4399813/150‑1 (MQ=255)
                                                                                             tAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTa                                                          <  1:5083436/150‑1 (MQ=255)
                                                                                              aaCTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTAt                                                         <  1:2046764/150‑1 (MQ=255)
                                                                                              aaCTGGACGCTGCAAGACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTAt                                                         <  2:3675784/150‑1 (MQ=255)
                                                                                               aCTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATg                                                        <  2:600075/150‑1 (MQ=255)
                                                                                                cTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGGGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGa                                                       >  2:7025287/1‑150 (MQ=255)
                                                                                                 tGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGaa                                                      >  2:4770944/1‑150 (MQ=255)
                                                                                                 tGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTGGAAAATTTTAGAGGGTACCGCGGCGGCCTTCCCCCGGGTCGGGCGGTGGCCGGGCACGCTGCGGACCGACACCCCGAGCAAAGGGCGGATGACGGGCAGTGATGaa                                                      >  1:2783035/1‑150 (MQ=255)
                                                                                                      gCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTGTAAATTTTAAGTGGGTAAGGATGCGGCATTCCACGGTTTATGGGGGTGGCCGGGCACGATTCTGAACGTCACCCCCCACAAAGGGGTGTTGACGGTCATTTTTGAAttgac                                                 >  1:1666822/1‑147 (MQ=255)
                                                                                                        tGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGATGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCgg                                               <  2:3070580/150‑1 (MQ=255)
                                                                                                              aCGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGAtt                                         >  2:4117106/1‑150 (MQ=255)
                                                                                                               cGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTc                                        <  1:1964189/150‑1 (MQ=255)
                                                                                                                  tttCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCTCGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGa                                     <  1:4765820/150‑1 (MQ=255)
                                                                                                                   ttCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGaa                                    <  1:2208502/150‑1 (MQ=255)
                                                                                                                   ttCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGaa                                    <  2:4635961/150‑1 (MQ=255)
                                                                                                                         aaaCCGTCCGAACAGTCGTTTAAAATTGAAGAGGGTACCGAAGCGGCAATACCCCGTTTATTGCTGTGGCCCAGCACCAAGCAGAAACTCACCCCCAACAAAGGGATGAAGACGGTAAATTAAGAAAAACCGGGGGATTCAGAAACAAcc                              >  2:5223014/1‑150 (MQ=255)
                                                                                                                                      aGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACg                 <  1:1772633/150‑1 (MQ=255)
                                                                                                                                      aGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACg                 <  1:6162425/150‑1 (MQ=255)
                                                                                                                                         cGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACGata              <  1:4953070/150‑1 (MQ=255)
                                                                                                                                           tttAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACGATAtt            >  1:4283029/1‑150 (MQ=255)
                                                                                                                                           tttAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACGATAtt            >  1:2569451/1‑150 (MQ=255)
                                                                                                                                           tttAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACGATAtt            <  2:3805929/150‑1 (MQ=255)
                                                                                                                                           tttAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACGATAtt            >  2:1104637/1‑150 (MQ=255)
                                                                                                                                           tttAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTAAAACTACCCTGCAAAACTACGATAtt            >  1:590343/1‑150 (MQ=255)
                                                                                                                                              aaaTTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACGATATTTAc         <  2:1993682/150‑1 (MQ=255)
                                                                                                                                                aTTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACGATATTTACtt       <  2:5743262/150‑1 (MQ=255)
                                                                                                                                                aTTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACGATATTTACtt       >  2:2862185/1‑150 (MQ=255)
                                                                                                                                                aTTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACGATATTTACtt       <  2:7185986/150‑1 (MQ=255)
                                                                                                                                                 tttGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACGATATTTACtac      >  2:5288438/1‑148 (MQ=255)
                                                                                                                                                  ttGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCa     <  1:3292766/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGATAACTATCTCGAGTGCTATCACTGTGGTCCGGCGCATCCAGGTTTCTCCGACTCCGTACAGGTTGATCGTTACTGGCACACCATGCACGGTAACTGGACGCTGCAATACGGTTTCGCCAAACCGTCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCA  >  NC_000913/1885273‑1885571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: