Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,090,122 A→G 5.7% intergenic (+76/‑70) hisL → / → hisG his operon leader peptide/ATP phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,090,1220AG 5.7% 1011.2 / 33.3 303intergenic (+76/‑70)hisL/hisGhis operon leader peptide/ATP phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (162/121);  new base G (11/6);  total (175/128)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

Only 100 of 304 total aligned reads displayed.

ATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGGCGCG  >  NC_000913/2089974‑2090271
                                                                                                                                                    |                                                                                                                                                     
aTAAACATTCACAGAGACTTTTAATACACGCGTTCAATTTAAACACCACCATCATCACAATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATATTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGAt                                                                                                                                                      >  1:6838029/1‑150 (MQ=255)
  aaaCATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCATCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCa                                                                                                                                                    >  1:2863692/1‑150 (MQ=255)
  aaaCATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCa                                                                                                                                                    >  1:1320004/1‑150 (MQ=255)
    aCATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCAcc                                                                                                                                                  <  2:4330205/150‑1 (MQ=255)
      aTTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCtt                                                                                                                                                >  1:631233/1‑150 (MQ=255)
           caGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCggg                                                                                                                                           <  2:3127085/150‑1 (MQ=255)
           caGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGGGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggg                                                                                                                                           >  2:5205107/1‑150 (MQ=255)
           caGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTGCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggg                                                                                                                                           <  2:4904297/150‑1 (MQ=255)
            agagACTTTTAGGACACGCGTGCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCCGGGGGGCCAGAACGCAGGAGAAAGCCCCCGGCCGATCACCTTCCgggg                                                                                                                                          >  2:7132760/1‑150 (MQ=255)
              agaCTTTTATGACACGCGTACAATTTAAACACCACCATCAACACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGACCGCATGAAAAAGCCCCCGGAAGGTGATCTTCCGGGGGc                                                                                                                                        <  1:642117/150‑1 (MQ=255)
                     tATGACACGCGTTCAAGTTAAACACCACCCTCATCACCATCATCCTGACGAGTCTGTCAGGCGAGGTGAGCAGGAAGACATTCAGATCTTCCAGTGGTGCATGACCGCATGAGAAAGCCCCCGGACGATCACCTTCCGGGGGCTAATTTa                                                                                                                                 <  2:3360705/150‑1 (MQ=255)
                      aTGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAt                                                                                                                                <  2:3535863/150‑1 (MQ=255)
                      aTGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAt                                                                                                                                <  1:6975553/150‑1 (MQ=255)
                      aTGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAt                                                                                                                                <  1:1521057/150‑1 (MQ=255)
                       tGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAtt                                                                                                                               >  1:6436443/1‑150 (MQ=255)
                           acGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc                                                                                                                           <  1:466030/150‑1 (MQ=255)
                             gcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCgg                                                                                                                         >  2:5404709/1‑150 (MQ=255)
                               gTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGtt                                                                                                                       <  1:1067757/150‑1 (MQ=255)
                                ttCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTg                                                                                                                      >  2:2429346/1‑150 (MQ=255)
                                ttCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTg                                                                                                                      >  2:1851355/1‑150 (MQ=255)
                                 tCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGa                                                                                                                     <  2:1032825/150‑1 (MQ=255)
                                    aTTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATaa                                                                                                                  >  1:3092906/1‑150 (MQ=255)
                                    aTTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATaa                                                                                                                  >  2:707825/1‑150 (MQ=255)
                                    aTTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATaa                                                                                                                  >  2:5704872/1‑150 (MQ=255)
                                    aTTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATaa                                                                                                                  >  2:1734452/1‑150 (MQ=255)
                                     tttAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAAc                                                                                                                 >  2:453383/1‑150 (MQ=255)
                                      ttAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACg                                                                                                                >  2:4974287/1‑150 (MQ=255)
                                      ttAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACg                                                                                                                >  1:3325508/1‑150 (MQ=255)
                                          acacCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTc                                                                                                            >  2:1652187/1‑150 (MQ=255)
                                           caccacCATCCGCACCATCACCCTGACCAGTCTGTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCa                                                                                                           <  1:5787001/150‑1 (MQ=255)
                                              caccatcatcaccatcatCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGAc                                                                                                        >  1:650149/1‑150 (MQ=255)
                                               accatcatcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACa                                                                                                       <  1:285273/150‑1 (MQ=255)
                                               accatcatcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACa                                                                                                       >  1:3471190/1‑150 (MQ=255)
                                               accatcatcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACa                                                                                                       <  1:1132282/150‑1 (MQ=255)
                                                ccatcctcaccatcagcCTGACGCGTCGGTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGCTCACCTTCCGGGGGCTTTTTTATTGCGCGGGTGATAACGGTTCAGACAg                                                                                                      <  2:5320261/150‑1 (MQ=255)
                                                ccatcatcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTGTTATTGCGCGGTTGATAACGGTTCAGACAg                                                                                                      >  2:3790742/1‑150 (MQ=255)
                                                 catcatcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTGATTGCGCGGTTGATAACGGTTCAGACAgg                                                                                                     >  1:3499113/1‑150 (MQ=255)
                                                   tcctcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGtt                                                                                                   <  2:1304708/147‑1 (MQ=255)
                                                   tcatcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTGTTTATTGCGCGGTTGATAACGGTTCAGACAGGtt                                                                                                   >  1:4191985/1‑150 (MQ=255)
                                                     atcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTa                                                                                                 <  2:4421767/150‑1 (MQ=255)
                                                      tcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTaa                                                                                                >  1:1794360/1‑150 (MQ=255)
                                                      tcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTaa                                                                                                >  1:5910341/1‑150 (MQ=255)
                                                       caccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTaaa                                                                                               <  2:2764660/150‑1 (MQ=255)
                                                         ccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAaga                                                                                             <  1:3416314/150‑1 (MQ=255)
                                                         ccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAaga                                                                                             <  2:6944533/150‑1 (MQ=255)
                                                         ccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAaga                                                                                             <  1:2609264/150‑1 (MQ=255)
                                                         ccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAaga                                                                                             <  1:3880574/150‑1 (MQ=255)
                                                          catcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAagag                                                                                            <  1:4455291/150‑1 (MQ=255)
                                                           atcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAgg                                                                                           <  2:8089/150‑1 (MQ=255)
                                                            tcatcCCGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGAGAACGGTTCAGACAGGTTTAAAGAGGa                                                                                          <  1:4810466/150‑1 (MQ=255)
                                                                cccgACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATaa                                                                                      <  2:6589197/147‑1 (MQ=255)
                                                                ccTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATaa                                                                                      >  1:6594581/1‑150 (MQ=255)
                                                                 cTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAAc                                                                                     >  1:2139844/1‑150 (MQ=255)
                                                                  tGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGCACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACa                                                                                    <  2:5530445/150‑1 (MQ=255)
                                                                      tAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAAt                                                                                >  2:2656505/1‑150 (MQ=255)
                                                                      tAGTCTGTCAGGCGATGTGGGCTGGAAGACATTCAGATCTTCCAGTGGTGCCTGAACGCATGTGAAAGCCGCCGGATGATCACCTTCCGGGGGATTTTTTTTTGCGCGGTTGATATCGGTTCTTACAGGTTTAAAGAGGAATAACaaatt                                                                                <  2:6835560/150‑3 (MQ=255)
                                                                           tttCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacag                                                                           >  2:1260183/1‑150 (MQ=255)
                                                                            gtCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacaga                                                                          <  1:5018087/149‑1 (MQ=255)
                                                                               cggCGAGGTGTGCTGCAAGACCTTCAGATCTTCCAGTGGGGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTATTCTGGCGCGGTTGATAACGGAGCAGACAGGTAAAAAGAGGACTAACAAAATgacagacac                                                                       >  2:5863445/2‑149 (MQ=255)
                                                                                       tgtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGtt                                                               >  1:54358/1‑150 (MQ=255)
                                                                                       tgtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGtt                                                               >  2:3486659/1‑150 (MQ=255)
                                                                                          gCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTAc                                                            <  1:2015022/150‑1 (MQ=255)
                                                                                           cTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACg                                                           >  2:4829052/1‑150 (MQ=255)
                                                                                            tGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGc                                                          >  1:4078166/1‑150 (MQ=255)
                                                                                            tGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTGTATTGCGCGGTTGATAACGGTTCAGACAGGTTTACAGAGGAATAACAAAATGACAGACAACACTCGTGTACGc                                                          >  2:5556290/1‑150 (MQ=255)
                                                                                              gAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCAt                                                        >  2:3988434/1‑150 (MQ=255)
                                                                                               aaGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATa                                                       >  1:1026295/1‑150 (MQ=255)
                                                                                                 gACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGc                                                     >  1:5281362/1‑150 (MQ=255)
                                                                                                  aCATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCt                                                    <  2:1494428/150‑1 (MQ=255)
                                                                                                  aCATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCt                                                    <  1:1915228/150‑1 (MQ=255)
                                                                                                        aGATCTTCCAGTGGTGCATGAACGCATGAGAATGCCCCCGGAAGTTCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGTTAACGGTTCAGAGAGGTTTTAAGAGGAATAACAAAATGAGAGACAACACTCGTTTACGCATAGCTATGCAg                                              >  1:2498769/1‑150 (MQ=255)
                                                                                                             ttCCAGTGGTGCATGAACGCATGAGCCAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGAGAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATc                                         <  1:2037258/150‑1 (MQ=255)
                                                                                                             ttCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATc                                         <  1:2691020/150‑1 (MQ=255)
                                                                                                               ccAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCATTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCg                                       <  1:5261145/150‑1 (MQ=255)
                                                                                                                   tggagCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTCTTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCg                                   >  2:2047549/1‑150 (MQ=255)
                                                                                                                        cATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACCGGGTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTaa                              >  1:5700391/1‑150 (MQ=255)
                                                                                                                        cATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGACTAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTaa                              >  2:4123413/1‑150 (MQ=255)
                                                                                                                        cATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTaa                              >  1:1773209/1‑150 (MQ=255)
                                                                                                                        cATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTaa                              >  2:2709983/1‑150 (MQ=255)
                                                                                                                         aTGAACGCATGAGAAAGCCCCCGGAAGTTCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACCGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTGTAAg                             >  1:7113314/1‑150 (MQ=255)
                                                                                                                         aTGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAg                             <  1:122605/150‑1 (MQ=255)
                                                                                                                          tGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTGAGACAGGTTTAAAGAGGAATAACAAAATTAAAGACAAAACTCGTTTACGCATAGCTATGCAGAAATACGGCCGTTTAAGt                            >  1:6056166/1‑150 (MQ=255)
                                                                                                                               gCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGtgatga                       <  1:484865/150‑1 (MQ=255)
                                                                                                                                aataaaaaaGCCCCCGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGAc                      >  2:3269422/7‑150 (MQ=39)
                                                                                                                                  tGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTc                    <  1:239814/150‑1 (MQ=255)
                                                                                                                                   aaaaaaGCCCCCGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTAAGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGTTGACTCa                   >  1:4605508/4‑150 (MQ=39)
                                                                                                                                      aaaGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTTCGCGGGTGTTAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAAAACACGTTTACGCAGAGCGATGCAGAAATACGGCCGGTTAAGTTATGACTCAcgc                >  1:3291424/1‑150 (MQ=255)
                                                                                                                                      aaaGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCAcgc                <  2:6052131/150‑1 (MQ=255)
                                                                                                                                         gCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGaa             <  2:2147975/150‑1 (MQ=255)
                                                                                                                                         gCCCCCGGAAGATCACCTTCCGGGGGCTTGTTTATTGCGCGGTTTATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGaa             <  2:4306278/150‑1 (MQ=255)
                                                                                                                                            cccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTg          <  1:772783/150‑1 (MQ=255)
                                                                                                                                            cccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTg          <  1:357157/150‑1 (MQ=255)
                                                                                                                                            cccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGAAAGGTTTAAATAGGAATAACAAAATGACAGAAAACACTCGTTTACGCCTAGCTATGCAGTAATCCGGCCGTTTAAGTGATTACACACGCGAATTg          >  2:643594/1‑150 (MQ=255)
                                                                                                                                            cccGGAAGATCAACTTCCGGGGGCTTTTTTCTTGCGCGGGTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAACTCCGGCCGTTTAAGTGATGACTCACGCGAATTg          >  2:6362014/1‑150 (MQ=255)
                                                                                                                                             ccGGAAGGTGATCTTCCGGGGGCTTTTTTATTTCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATTACAGACAACACGCGTTTAAGCAAAGCTATGCAGAAATCCGGCCGTTTAAGTGATTTCTAACGCGAAtttc         >  1:2633541/1‑148 (MQ=255)
                                                                                                                                             ccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGc         >  2:2290549/1‑150 (MQ=255)
                                                                                                                                              cGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCt        <  1:6013978/150‑1 (MQ=255)
                                                                                                                                              cGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCt        <  1:4163404/150‑1 (MQ=255)
                                                                                                                                               ggAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTg       <  2:4662135/150‑1 (MQ=255)
                                                                                                                                                   gATCACCTTCCGGGGGCTTTTTTATTTCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACACGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGAATAACGCGAATTGATGgcgc   >  1:6167113/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGGCGCG  >  NC_000913/2089974‑2090271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: