Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,090,124 C→G 5.5% intergenic (+78/‑68) hisL → / → hisG his operon leader peptide/ATP phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,090,1240CG 5.5% 1115.3 / 44.8 307intergenic (+78/‑68)hisL/hisGhis operon leader peptide/ATP phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (170/120);  new base G (11/6);  total (181/126)
Fisher's exact test for biased strand distribution p-value = 8.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 309 total aligned reads displayed.

TAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGGCGCGC  >  NC_000913/2089975‑2090272
                                                                                                                                                     |                                                                                                                                                    
 aaaCATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCATCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCa                                                                                                                                                     >  1:2863692/1‑150 (MQ=255)
 aaaCATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCa                                                                                                                                                     >  1:1320004/1‑150 (MQ=255)
     aTTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCtt                                                                                                                                                 >  1:631233/1‑150 (MQ=255)
     aTTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCtt                                                                                                                                                 >  2:5972601/1‑150 (MQ=255)
          caGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCggg                                                                                                                                            <  2:3127085/150‑1 (MQ=255)
          caGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGGGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggg                                                                                                                                            >  2:5205107/1‑150 (MQ=255)
          caGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTGCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggg                                                                                                                                            <  2:4904297/150‑1 (MQ=255)
           agagACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgggg                                                                                                                                           >  2:6065082/1‑150 (MQ=255)
           agagACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGCCGAGGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgggg                                                                                                                                           >  2:1519680/1‑150 (MQ=255)
           agagACTTTTAGGACACGCGTGCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCCGGGGGGCCAGAACGCAGGAGAAAGCCCCCGGCCGATCACCTTCCgggg                                                                                                                                           >  2:7132760/1‑150 (MQ=255)
           agagACTTTAATGACACGCGTTCAATTTAAACAACACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgggg                                                                                                                                           >  1:5615264/1‑150 (MQ=255)
            gagaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg                                                                                                                                          >  2:205819/1‑150 (MQ=255)
             agaCTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGGTGATCTTCCGGGGGc                                                                                                                                         <  1:4651941/150‑1 (MQ=255)
             agaCTTTTATGACACGCGTACAATTTAAACACCACCATCAACACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGACCGCATGAAAAAGCCCCCGGAAGGTGATCTTCCGGGGGc                                                                                                                                         <  1:642117/150‑1 (MQ=255)
                  tttATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCttttt                                                                                                                                    >  2:1260795/1‑150 (MQ=255)
                    tATGACACGCGTTCAAGTTAAACACCACCCTCATCACCATCATCCTGACGAGTCTGTCAGGCGAGGTGAGCAGGAAGACATTCAGATCTTCCAGTGGTGCATGACCGCATGAGAAAGCCCCCGGACGATCACCTTCCGGGGGCTAATTTa                                                                                                                                  <  2:3360705/150‑1 (MQ=255)
                     aTGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAt                                                                                                                                 <  1:1521057/150‑1 (MQ=255)
                     aTGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAt                                                                                                                                 <  2:3535863/150‑1 (MQ=255)
                      tGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAtt                                                                                                                                >  1:6436443/1‑150 (MQ=255)
                           cgcgTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGcgcg                                                                                                                           <  1:4140772/150‑1 (MQ=255)
                              gTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGtt                                                                                                                        <  1:1067757/150‑1 (MQ=255)
                               ttCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTg                                                                                                                       >  2:2429346/1‑150 (MQ=255)
                                  aaTTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATa                                                                                                                    >  2:2654701/1‑150 (MQ=255)
                                   aTTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATaa                                                                                                                   >  1:3092906/1‑150 (MQ=255)
                                   aTTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATaa                                                                                                                   >  2:707825/1‑150 (MQ=255)
                                    tttAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAAc                                                                                                                  >  2:453383/1‑150 (MQ=255)
                                     ttAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACg                                                                                                                 >  1:3325508/1‑150 (MQ=255)
                                     ttAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACg                                                                                                                 >  2:4974287/1‑150 (MQ=255)
                                      tAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACgg                                                                                                                >  2:2691020/1‑150 (MQ=255)
                                        aaCACCACCATCATCACCATCAGCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGtt                                                                                                              <  1:6150854/150‑1 (MQ=255)
                                         acacCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTc                                                                                                             <  1:261378/150‑1 (MQ=255)
                                         acacCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTc                                                                                                             >  1:5780914/1‑150 (MQ=255)
                                             caccatcatcaccatcatCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGAc                                                                                                         >  1:650149/1‑150 (MQ=255)
                                              accatcatcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACa                                                                                                        <  1:285273/150‑1 (MQ=255)
                                              accatcatcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACa                                                                                                        <  1:1132282/150‑1 (MQ=255)
                                              accatcatcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTGTTATTGCGCGGTTGATAACGGTTCAGAcc                                                                                                        >  2:1040570/1‑149 (MQ=255)
                                               ccatcatcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTGTTATTGCGCGGTTGATAACGGTTCAGACAg                                                                                                       >  2:3790742/1‑150 (MQ=255)
                                                catcatcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAgg                                                                                                      >  1:3499575/1‑150 (MQ=255)
                                                   catcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGttt                                                                                                   <  1:5965462/150‑1 (MQ=255)
                                                    atcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTa                                                                                                  <  2:4421767/150‑1 (MQ=255)
                                                     tcaccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTaa                                                                                                 >  1:1794360/1‑150 (MQ=255)
                                                      caccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTaaa                                                                                                <  2:2764660/150‑1 (MQ=255)
                                                        ccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAaga                                                                                              <  1:3416314/150‑1 (MQ=255)
                                                        ccatcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAaga                                                                                              <  2:6944533/150‑1 (MQ=255)
                                                          atcatcCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAgg                                                                                            <  2:8089/150‑1 (MQ=255)
                                                           tcatcCTGACTAGTCGGTCAGGCGAGGTGTGCTGGAAGACAGTCAGATCTTCCAGTGGGGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGa                                                                                           <  1:3486679/150‑1 (MQ=255)
                                                               cccgACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATaa                                                                                       <  2:6589197/147‑1 (MQ=255)
                                                               ccTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATaa                                                                                       >  1:6594581/1‑150 (MQ=255)
                                                                cTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAAc                                                                                      >  2:1894780/1‑150 (MQ=255)
                                                                cTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAAc                                                                                      >  1:2139844/1‑150 (MQ=255)
                                                                     tAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTATTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAAt                                                                                 >  2:4328898/1‑150 (MQ=255)
                                                                     tAGTCTGTCAGGCGATGTGGGCTGGAAGACATTCAGATCTTCCAGTGGTGCCTGAACGCATGTGAAAGCCGCCGGATGATCACCTTCCGGGGGATTTTTTTTTGCGCGGTTGATATCGGTTCTTACAGGTTTAAAGAGGAATAACaaatt                                                                                 <  2:6835560/150‑3 (MQ=255)
                                                                        tCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGTGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTATAGAGGAATAACAAAATgtc                                                                              >  1:6036261/1‑148 (MQ=255)
                                                                          tttCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacag                                                                            >  2:1764260/1‑150 (MQ=255)
                                                                            tCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATgacagac                                                                          >  2:5403559/1‑150 (MQ=255)
                                                                               ggCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGacaac                                                                       >  1:988007/1‑150 (MQ=255)
                                                                                gCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGacaaca                                                                      >  1:4357839/1‑150 (MQ=255)
                                                                                     gtgtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCATACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGt                                                                 <  1:2654701/150‑1 (MQ=255)
                                                                                      tgtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGtt                                                                >  1:2562008/1‑150 (MQ=255)
                                                                                      tgtgCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGtt                                                                >  2:3486659/1‑150 (MQ=255)
                                                                                         gCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTAc                                                             <  1:2015022/150‑1 (MQ=255)
                                                                                          cTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACg                                                            >  2:4829052/1‑150 (MQ=255)
                                                                                           tGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGc                                                           >  1:6779728/1‑150 (MQ=255)
                                                                                           tGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTGTATTGCGCGGTTGATAACGGTTCAGACAGGTTTACAGAGGAATAACAAAATGACAGACAACACTCGTGTACGc                                                           >  2:5556290/1‑150 (MQ=255)
                                                                                             gAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCAt                                                         >  2:3988434/1‑150 (MQ=255)
                                                                                              aaGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATa                                                        >  1:1026295/1‑150 (MQ=255)
                                                                                                gACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGc                                                      >  1:4181865/1‑150 (MQ=255)
                                                                                                 aCATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCt                                                     <  1:1915228/150‑1 (MQ=255)
                                                                                                    ttCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTTCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATg                                                  >  1:49647/1‑150 (MQ=255)
                                                                                                       aGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGGTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACACGTTTACGCATAGCTATGCAg                                               >  1:2452380/1‑150 (MQ=255)
                                                                                                            ttCCAGTGGTGCATGAACGCATGAGCCAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGAGAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATc                                          <  1:2037258/150‑1 (MQ=255)
                                                                                                            ttCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATc                                          >  1:7139164/1‑150 (MQ=255)
                                                                                                            ttCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATc                                          >  1:5155223/1‑150 (MQ=255)
                                                                                                            ttCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATc                                          <  1:2691020/150‑1 (MQ=255)
                                                                                                            ttCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTGGATTGCGCGGTTGATAACGGTTCAGACAGGTTTAACGAGGAATAACAAAATGACAGACAACACTCGTGTACGCATAGCTATGCAGAAATc                                          >  2:1653533/1‑150 (MQ=255)
                                                                                                              ccAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCg                                        >  1:4773024/1‑150 (MQ=255)
                                                                                                                  tggtgCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCg                                    >  1:5869866/1‑150 (MQ=255)
                                                                                                                  tggtgCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCg                                    >  1:7046090/1‑150 (MQ=255)
                                                                                                                    gtgCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGGATAACAAAATGACAGACAACACTCGTTTACGCATAGCGATGCAGAAATCCGGCCGtt                                  >  2:5528276/1‑150 (MQ=255)
                                                                                                                       cATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTaa                               >  1:1773209/1‑150 (MQ=255)
                                                                                                                        aTGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAg                              <  1:122605/150‑1 (MQ=255)
                                                                                                                            ccGCATGAGAAAGCCCCCGGAAGATCACCGTCCGGGGGCTTTTTTATTGTGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGtgat                          <  2:4085566/149‑1 (MQ=255)
                                                                                                                              gCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGtgatga                        <  1:484865/150‑1 (MQ=255)
                                                                                                                               aataaaaaaGCCCCCGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGAc                       >  2:3269422/7‑150 (MQ=39)
                                                                                                                                  aaaaaaGCCCCCGGAAGGTGATCTTCCGGGGGCTTTTTTATTGCGCGGTTGGTAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGTTGTCTCa                    >  1:4553253/4‑150 (MQ=39)
                                                                                                                                     aaaGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTTCGCGGGTGTTAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAAAACACGTTTACGCAGAGCGATGCAGAAATACGGCCGGTTAAGTTATGACTCAcgc                 >  1:3291424/1‑150 (MQ=255)
                                                                                                                                     aaaGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCAcgc                 >  2:5713846/1‑150 (MQ=255)
                                                                                                                                        gCCCCCGGATGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGATCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGaa              <  2:4306200/150‑1 (MQ=255)
                                                                                                                                          ccccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAAtt            <  1:5427641/150‑1 (MQ=255)
                                                                                                                                           cccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTg           <  1:357157/150‑1 (MQ=255)
                                                                                                                                           cccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGAAAGACAACACACGTTTACGCATAGCTATGCAGAAATACGGCCGTTTAAGTGATGACACACGCGAAttt           >  1:405939/1‑149 (MQ=255)
                                                                                                                                           cccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGAAAGGTTTAAATAGGAATAACAAAATGACAGAAAACACTCGTTTACGCCTAGCTATGCAGTAATCCGGCCGTTTAAGTGATTACACACGCGAATTg           >  2:643594/1‑150 (MQ=255)
                                                                                                                                           cccGGAAGATCAACTTCCGGGGGCTTTTTTCTTGCGCGGGTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAACTCCGGCCGTTTAAGTGATGACTCACGCGAATTg           >  2:6362014/1‑150 (MQ=255)
                                                                                                                                            ccGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGc          >  2:2290549/1‑150 (MQ=255)
                                                                                                                                             cGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCt         <  1:5274191/150‑1 (MQ=255)
                                                                                                                                              ggAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTg        <  2:4662135/150‑1 (MQ=255)
                                                                                                                                                    tCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGgcgcgc  >  2:5314354/1‑150 (MQ=255)
                                                                                                                                                    tCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGgcgcgc  >  2:222688/1‑150 (MQ=255)
                                                                                                                                                    tCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGgcgcgc  >  2:1412203/1‑150 (MQ=255)
                                                                                                                                                    tCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACACGTTTACGCCTAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTTCTGgcgcgc  >  1:3872275/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TAAACATTCACAGAGACTTTTATGACACGCGTTCAATTTAAACACCACCATCATCACCATCATCCTGACTAGTCTTTCAGGCGATGTGTGCTGGAAGACATTCAGATCTTCCAGTGGTGCATGAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCGGTTGATAACGGTTCAGACAGGTTTAAAGAGGAATAACAAAATGACAGACAACACTCGTTTACGCATAGCTATGCAGAAATCCGGCCGTTTAAGTGATGACTCACGCGAATTGCTGGCGCGC  >  NC_000913/2089975‑2090272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: