Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,279 C→A 6.3% intergenic (‑75/+233) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2790CA 6.3% 1143.6 / 13.3 374intergenic (‑75/+233)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (155/177);  new base A (31/8);  total (189/185)
Fisher's exact test for biased strand distribution p-value = 1.35e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 382 total aligned reads displayed.

AATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTC  >  NC_000913/2177130‑2177428
                                                                                                                                                     |                                                                                                                                                     
    ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACaa                                                                                                                                                   >  2:1805326/1‑150 (MQ=255)
     tGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaa                                                                                                                                                  >  2:7202574/1‑150 (MQ=255)
     tGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTGAAAATATAATTAAATTATTTTTTTTTAAATATAGAGATAAAGATaaaaaa                                                                                                                                                  >  2:5143444/1‑150 (MQ=255)
       aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTGAAAATATAAAGAAATTATTTGTTTTTAAATATAGATATAAAGATAACaaaaa                                                                                                                                                >  2:5595427/1‑150 (MQ=255)
       aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATAACaaaaa                                                                                                                                                >  2:6564305/1‑150 (MQ=255)
       aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                                                                >  2:4752221/1‑150 (MQ=255)
       aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGATAACaaaaa                                                                                                                                                >  2:2098335/1‑150 (MQ=255)
       aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATCTCCTGTCGTGTGTTTTCGATTTCCAAATATAATGAAATTAATTGTTTTTAAATATCGAGCTAACGCTCCCcaaaa                                                                                                                                                >  2:683328/1‑150 (MQ=255)
           ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCGTTGTCGATACCACGTACAGTTTCATATCCTGTCGTGGGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTTAATATCGAGATAACGATCACACAAACGAc                                                                                                                                            >  1:6162146/1‑150 (MQ=255)
            cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAATATAATGAAAATATTTTTTTTTAAATATAGAGATAAAGATCAAAAAAAAGACa                                                                                                                                           >  1:2295047/1‑150 (MQ=255)
            cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTGAAAATATAATTAAAATATTTTTTTTTTAATATAGAGATAACGATAACAAAAAAGaaa                                                                                                                                           >  1:5684549/1‑148 (MQ=255)
            cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGaaa                                                                                                                                           >  2:3124323/1‑148 (MQ=255)
             ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTTAATATCGAGATAACGATAACAAAAAAGACaa                                                                                                                                          >  1:4221649/1‑150 (MQ=255)
                   gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAAAATAAATAAATAATTTTTTTTTAAATATAGAGAAAAAGATCAAAAAAACGACAATAAGaa                                                                                                                                    >  2:3418954/1‑150 (MQ=255)
                   gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTGGAAATATTATGTAATTATTTTTTTTTTAATATGGAGGTAAAGCTCACAAAAACGACAAAATgga                                                                                                                                    >  1:2500346/1‑148 (MQ=255)
                   gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTACAAAATATAATGAAATAATTTGTTTTTAAATATAGATATAACAATCACAAAAACGACAAAATTaa                                                                                                                                    >  1:6054212/1‑150 (MQ=255)
                    cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAAATATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAAAATGaaa                                                                                                                                   >  2:6911086/1‑150 (MQ=255)
                         ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGGTTTCGAATTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattat                                                                                                                              >  2:6631527/1‑150 (MQ=255)
                           gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTc                                                                                                                            <  2:3340408/150‑1 (MQ=255)
                               cgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAATTATTAGTTTTTAAATATAGAGATAAAGATAACAAAAAAGAAAATATTTAAATTTTTAgatg                                                                                                                        >  1:1056016/1‑148 (MQ=255)
                                gcTGTGCGTTGTTCAGCTTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATACCGATCACAAAAACGACAATATGAAAATTATTCgagga                                                                                                                       <  2:2315756/150‑1 (MQ=255)
                                    tgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAAAATAATGAAATTATTTTTTTTTAAATATAGAGATAACGAAAACAAAAAAGAAAAAAATAAAAATATTGGAGGAGTgg                                                                                                                   >  1:4737262/1‑149 (MQ=255)
                                       gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAg                                                                                                                >  2:6396255/1‑150 (MQ=255)
                                       gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAATAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAACGACAATATGAAAATTATTAGAGGAGTGAAAg                                                                                                                >  2:4424565/1‑150 (MQ=255)
                                          gttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCa                                                                                                             <  2:1524677/150‑1 (MQ=255)
                                           ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATAAAAAGAAAATAATTTTTTTTTAAAATAGAGATAAAGATAAAAAAAACAACAAAAAGAAAAATATAATAGGAGTTAAAAGCaa                                                                                                            >  1:1346555/1‑150 (MQ=255)
                                             cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACCATAACAAAAACGACAATATGAAAATTATTCGAGGTGTGAAAGGCaaaa                                                                                                          >  2:3828361/1‑150 (MQ=255)
                                             cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAAATAAAAAATTTTTTTTTAAAAAAAGAGATAAAAATAAAAAAAAAAACAAAAAGAAAAATATTAGAGGGGTGGAAGGCaaaa                                                                                                          >  1:4393979/1‑150 (MQ=255)
                                              aGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATAAAGAGAAAAAGAAAACAAAAACAACAATATGAAAATTATTAGAGGAGGTAAAGGCaaaaa                                                                                                         >  2:2472153/1‑150 (MQ=255)
                                              aGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaa                                                                                                         <  2:5269716/150‑1 (MQ=255)
                                                 aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                                                      <  2:5383254/150‑1 (MQ=255)
                                                 aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAAAATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                                                      >  2:3052954/1‑150 (MQ=255)
                                                  tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAAGACAAAATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACg                                                                                                     >  2:1684045/1‑150 (MQ=255)
                                                    tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGc                                                                                                   <  2:4200478/150‑1 (MQ=255)
                                                           tctaTACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                                                                            <  2:2793802/147‑1 (MQ=255)
                                                            cGATACCACGTACTTTTTCTTATCGTGTGGTTTGTTTTGGATTTCAAAATATACTGAATTTTTTTGTTTTTAAATTTGGAGATAAGGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAt                                                                                           <  2:6685076/150‑1 (MQ=255)
                                                            cGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAt                                                                                           <  2:1821531/150‑1 (MQ=255)
                                                             taTACCACTTAATTTTTAATATCGTTTTGTTTTTTTTCTATTTAAAAATATAATTAATTTTTTTTTTTTTAAATATCGATATAACTATCACAAAAACGACAATATGAAAATTTTTCGAGGAGTGAAAGGAAAAAAAACGGCCTCCCGATa                                                                                          <  1:2398349/149‑1 (MQ=255)
                                                                  cACTTACATTTTAATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                                                                     <  2:1257220/150‑1 (MQ=255)
                                                                  cACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                                                                     <  2:3447058/150‑1 (MQ=255)
                                                                     ttACTTTTTCTTATCCTGTCTTTTGTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                                                                  <  2:5090831/149‑1 (MQ=255)
                                                                      tACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAAAATCGAGATAAAGAAAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAATGCAAAAAAAAGGCCTCCCGATAGGGAAGCCg                                                                                 >  2:2868923/1‑150 (MQ=255)
                                                                        atttttAATATCCTGTCGTTTTTTTTCTATTTAAAAATATAATTAAATTTTTTGTTTTTAAATATCTATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                                                               <  1:1095900/148‑1 (MQ=255)
                                                                        attttaCATACCCTTTGTTTTTTTTTTTATTAAAAAATATATTTAATTTTTTTTTTTTTAAATTTCTACAAAACTAAAAAAAAAACGAAAATATTAAAATTATTCGAGGATTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                                                               <  1:2485585/148‑1 (MQ=255)
                                                                         aTTTACATATCCTGTCGTTTTTTTTCTATTTAAAAAAATAATCAAATTTTTTTTTTTTAAATATCTAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                                                                              <  1:6314090/150‑1 (MQ=255)
                                                                                   cttGTCTTTTTTTTTCGATTTAAAAATATAATGAATTTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                                                                    <  1:5486797/148‑1 (MQ=255)
                                                                                   ccTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGATATAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                                                                    <  1:3620024/150‑1 (MQ=255)
                                                                                         gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                                                              <  2:7138244/150‑1 (MQ=255)
                                                                                         gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                                                              <  2:2617803/150‑1 (MQ=255)
                                                                                          tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCGAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                                                             >  2:878716/1‑150 (MQ=255)
                                                                                          tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGAGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAACTGCGCGTGtttt                                                             >  2:4938614/1‑150 (MQ=255)
                                                                                           ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCACAGTGCGCGTGttttt                                                            >  1:3828898/1‑150 (MQ=255)
                                                                                           ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt                                                            >  2:676390/1‑150 (MQ=255)
                                                                                           ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt                                                            <  1:4038770/150‑1 (MQ=255)
                                                                                            tgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa                                                           <  2:4051045/150‑1 (MQ=255)
                                                                                             gtttTCGATTTCAAAATATAATGAATTTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                                                          <  2:595363/150‑1 (MQ=255)
                                                                                              ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                                                         >  1:435351/1‑150 (MQ=255)
                                                                                              ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                                                         >  1:5622605/1‑150 (MQ=255)
                                                                                              ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                                                         >  1:3472025/1‑150 (MQ=255)
                                                                                              ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                                                         >  1:6003652/1‑150 (MQ=255)
                                                                                                ttCGATTTCAAAATATCAGGAACTTATTTGTTTTTAAATATCGCGATAACGATCACAACCACGACAATATGAAAATTCTTCGAGGAGTGAAAGGCAAAAAAACGGCCGCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                                                       <  2:3876945/150‑1 (MQ=255)
                                                                                                ttCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                                                       <  1:2972595/150‑1 (MQ=255)
                                                                                                 ccGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                                                      >  1:2639858/2‑150 (MQ=255)
                                                                                                   gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAATCGACAATATGTTAATTATTCGTGGAGTGAATGGCAAATTTTCGGCGTCCCGTTTGGGAAGCCGTAGCAAAGTGCGGGTGTTTTTATGCgggt                                                    >  2:2993532/1‑149 (MQ=255)
                                                                                                   gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                                                    >  1:5749854/1‑150 (MQ=255)
                                                                                                      ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc                                                 >  1:1160736/1‑150 (MQ=255)
                                                                                                       tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                                >  1:1632453/1‑150 (MQ=255)
                                                                                                       tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                                <  1:3309458/150‑1 (MQ=255)
                                                                                                       tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                                <  1:2104812/150‑1 (MQ=255)
                                                                                                        cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg                                               <  2:3658828/150‑1 (MQ=255)
                                                                                                         aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt                                              >  1:2041471/1‑150 (MQ=255)
                                                                                                           aaTATACTGAAATTATTTGTTTTTAACTATCGAGATACCGATCACAACCACGACAATATGAAAATTATTCGAGGAGTGACCGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt                                            <  2:6162078/150‑1 (MQ=255)
                                                                                                            ataaAATTAAATTATTTGTTTTAAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa                                           <  1:6911086/150‑1 (MQ=255)
                                                                                                             tataATGAAATTATTTGTTTTTAAATATCGAGATACCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa                                          <  1:1881697/150‑1 (MQ=255)
                                                                                                              ataATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa                                         <  1:3259034/150‑1 (MQ=255)
                                                                                                              ataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa                                         <  2:4669527/150‑1 (MQ=255)
                                                                                                               taATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc                                        <  2:3235572/150‑1 (MQ=255)
                                                                                                               taATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc                                        <  2:5487388/150‑1 (MQ=255)
                                                                                                                  tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa                                     >  1:5151456/1‑150 (MQ=255)
                                                                                                                  tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa                                     <  2:5684549/150‑1 (MQ=255)
                                                                                                                   gAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAc                                    >  1:5023745/1‑150 (MQ=255)
                                                                                                                     aaTTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGt                                  <  2:190555/150‑1 (MQ=255)
                                                                                                                      attattTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTg                                 >  1:193517/1‑150 (MQ=255)
                                                                                                                       ttattTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTgg                                <  1:3709683/150‑1 (MQ=255)
                                                                                                                       ttattTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTgg                                >  1:2758503/1‑150 (MQ=255)
                                                                                                                         ttttGTTTTTAAATATCGAGATAATGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCa                              <  2:35838/149‑1 (MQ=255)
                                                                                                                         attTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCa                              <  2:1042409/150‑1 (MQ=255)
                                                                                                                           ttGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGGTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGt                            >  2:111713/1‑150 (MQ=255)
                                                                                                                               ttttAAATATCGAGATAACGATCACAAAAACGACATTATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTa                        >  1:5089619/1‑150 (MQ=255)
                                                                                                                                tttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTAt                       >  2:6313562/1‑150 (MQ=255)
                                                                                                                                 ttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATg                      <  2:2242573/150‑1 (MQ=255)
                                                                                                                                 ttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATg                      >  1:4565088/1‑150 (MQ=255)
                                                                                                                                     atatCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAgg                  <  2:3934752/150‑1 (MQ=255)
                                                                                                                                       atCGAGATAACGATCACAAAAACGACAATATGAACATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCACCCGAGAGGGAAGCCGTAGCAAAGTGCGCGAGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTCCTCTGTAGGCa                >  1:4281275/1‑150 (MQ=255)
                                                                                                                                        tCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCACAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCAt               >  2:1388356/1‑150 (MQ=255)
                                                                                                                                        tCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCAt               <  1:6595175/150‑1 (MQ=255)
                                                                                                                                          gagaTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGa             >  1:3737868/1‑150 (MQ=255)
                                                                                                                                             aTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATaa          <  2:5752257/150‑1 (MQ=255)
                                                                                                                                             aTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATaa          <  1:897190/150‑1 (MQ=255)
                                                                                                                                                  gATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAGAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGCAGGCATGATAAGACGc     >  2:2489760/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTC  >  NC_000913/2177130‑2177428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: