Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,304,798 T→A 5.0% intergenic (+405/+310) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,304,7980TA 5.0% 674.9 / 5.3 264intergenic (+405/+310)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base T (131/105);  new base A (15/2);  total (153/111)
Fisher's exact test for biased strand distribution p-value = 9.51e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 266 total aligned reads displayed.

GGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTT  >  NC_000913/2304650‑2304947
                                                                                                                                                     |                                                                                                                                                     
   tAAGGCGGTTACGCCGCATCCGACATCCAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGa                                                                                                                                                   <  2:5724405/150‑1 (MQ=37)
   caaGGCGGTCACGCCGCATCCGACATCCAACGCCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGa                                                                                                                                                   <  1:5244522/149‑1 (MQ=12)
     aGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGc                                                                                                                                                 <  1:5033724/150‑1 (MQ=12)
       gcgtaaccgcctcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGGCGCTGGCGCGGCTGCTCAGGCCTACACCGCTGTGAACTGCTCCACCCCGGAGGTTGGATACGGCCGTTACGCCGCATCCGACATCCAACGTCCGAGCCg                                                                                                                                               >  2:4697669/8‑150 (MQ=11)
            tACGCCGCATCCGACATCTATCGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTTTCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGc                                                                                                                                          <  1:5560157/150‑1 (MQ=255)
              cgccgcATCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCt                                                                                                                                        <  1:3198504/150‑1 (MQ=21)
               gccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTg                                                                                                                                       >  1:1675778/1‑150 (MQ=37)
               gccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTg                                                                                                                                       >  2:2570963/1‑150 (MQ=21)
               gccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGGTACGCCGCAGCCGACCTCCAACCACCGAGACCGTTGCCTg                                                                                                                                       >  1:4878144/1‑150 (MQ=11)
                ccgcATCCGACATCAAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGa                                                                                                                                      >  1:1175292/1‑150 (MQ=37)
                  gcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACCCCCAACGTCCGAGCCGGTTGCCTGATg                                                                                                                                    <  1:1792168/150‑1 (MQ=255)
                     tCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGa                                                                                                                                 <  1:6355926/150‑1 (MQ=37)
                        gACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGACGTGCGCCCCCCCGGAGGTTGGATAAGGCGGGTACGCCGCATCCGACATCCACCGGCCGCGCCGGTGGCCTGAGGcgccgc                                                                                                                              >  1:3412334/1‑150 (MQ=11)
                           aTCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACCCCGCTGGGACGTGCTCCACCCCGTAGGTTGGTGTAGGCGGTTACGCCGCATCCGACATCAAACGTCCGAGCCGGGTGCCTGATGCGACGCTgg                                                                                                                           >  2:7227877/1‑150 (MQ=11)
                            tCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCCATACACACCTCTGCCGTGCGACACCCCGTATGTGGGATCAGTCGGTAACGCCGCCTCCGACATCGAACGGCCCAGCCGGGTGACTGATGCGACGCTGgc                                                                                                                          >  2:6093999/1‑150 (MQ=2)
                              tacctccccAGCCGGTTGGCCTCTGCGACGCTGGCGGTCGCTCTCCGGCCCTCACCGCTGTGAAGTGCTGCACCCCGTAGGTCCGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                        <  2:5017512/145‑1 (MQ=11)
                              tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCAAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                        >  1:5003720/1‑150 (MQ=21)
                                aCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                      <  2:834734/150‑1 (MQ=37)
                                aCGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                      <  2:2961323/150‑1 (MQ=37)
                                aCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                      <  1:3840466/150‑1 (MQ=37)
                                 cGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTc                                                                                                                     <  1:5124927/150‑1 (MQ=37)
                                  gCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAACAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTCGGTTGGCTAAGGCCGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCt                                                                                                                    >  1:5689222/1‑150 (MQ=255)
                                       caccGGTTGACAGAAGAGACGCTGTCCCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTACGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCa                                                                                                               <  1:1049049/148‑1 (MQ=11)
                                       aGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTTGATAATGCTGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTTCCTGATGCGACGCTGGCGCGTCTTTTCa                                                                                                               <  1:663829/150‑1 (MQ=11)
                                        cccGGGTGCCTGCGGCGACGCTGGCGCGTCTGAGCCGGCCGACACCGCTGTGAAGTGCTCCACCCCGTAGGTGGGATACGGCGGGTCCGCCGCCTCCGCCCTCCCACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAg                                                                                                              <  1:6483517/149‑1 (MQ=11)
                                         ccGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAgg                                                                                                             <  2:2690757/150‑1 (MQ=38)
                                               gCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTaca                                                                                                       >  2:1933248/1‑150 (MQ=39)
                                               gCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTaca                                                                                                       <  2:2598012/150‑1 (MQ=39)
                                                 cTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAcc                                                                                                     <  1:1523433/150‑1 (MQ=39)
                                                 cTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCAAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGGCTACGcc                                                                                                     >  1:2440233/1‑150 (MQ=255)
                                                  tGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCg                                                                                                    >  1:5523192/1‑150 (MQ=37)
                                                    aTGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCt                                                                                                  <  1:7106611/150‑1 (MQ=39)
                                                      gCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtgt                                                                                                <  2:1175292/150‑1 (MQ=39)
                                                       cGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTAAACCCCGTAGGTTGGATAAGTCGGTAACGCCGCATCCCACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCATACACCCCtcag                                                                                               >  2:4457973/1‑147 (MQ=11)
                                                          cGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAg                                                                                            >  1:5095979/1‑150 (MQ=39)
                                                          cGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAg                                                                                            <  2:6619110/150‑1 (MQ=39)
                                                          cGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCGTATCAGGCCTACACCGCTGTGAAg                                                                                            >  1:6987958/1‑150 (MQ=255)
                                                             gggCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGc                                                                                         <  2:5357243/149‑1 (MQ=39)
                                                              ggCGCGTCTTATCAGGCCTACACCGCTGTGTAGTGCTCCACCCCGTAGGTTGGATTAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCt                                                                                        <  2:5638906/150‑1 (MQ=255)
                                                               ccgcgTCTTTTCCGGCCTACACCGCTGTTAAGTGCTCCACCCCTTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCGGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTc                                                                                       <  1:6796105/149‑1 (MQ=14)
                                                                  cgTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCAc                                                                                    >  2:6962593/1‑150 (MQ=39)
                                                                  cgTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCAc                                                                                    >  2:6962280/1‑150 (MQ=39)
                                                                       ttATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCg                                                                                >  1:1339358/1‑150 (MQ=37)
                                                                         aTCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTa                                                                              <  1:7109644/150‑1 (MQ=39)
                                                                          tCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAg                                                                             >  1:1079430/1‑150 (MQ=39)
                                                                            aGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGt                                                                           >  1:4188366/1‑150 (MQ=39)
                                                                              gCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCg                                                                         >  2:5863881/1‑150 (MQ=40)
                                                                              gCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCACTCGCCCCCTGAGAAGTGCTAAACCCCGTGGGTCg                                                                         >  1:2658469/1‑150 (MQ=11)
                                                                               ccTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCGAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGAGTACACAGCTGTGTCGTGCTCGTCCCCTTTGGTCgg                                                                        >  2:1844565/1‑150 (MQ=14)
                                                                                cTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCAATACACCCCTCAGAAGTGCACAACCCCGTATGTCGGa                                                                       >  1:3547924/1‑150 (MQ=14)
                                                                                   cacCGCTGTGAAGTGCTCCACCCCGTAGGTAGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCACGCCTACACCGCTGTGAAGTGCTCCACCACGTAGCTCGGATaa                                                                    >  2:4512447/1‑150 (MQ=11)
                                                                                        cTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGAGAAGTGCTCCACCCCGTAGGTCGGAGAGGGCgg                                                               >  2:3805111/1‑150 (MQ=255)
                                                                                        cTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                        >  1:6439546/1‑93 (MQ=18)
                                                                                         tgtgAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGt                                                              <  1:208730/150‑1 (MQ=17)
                                                                                          gtgAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTg                                                                                                                                       >  1:3340625/1‑76 (MQ=255)
                                                                                           tgAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCtt                                                                                                                   <  2:2859855/95‑1 (MQ=40)
                                                                                             aaGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGGCCGAGCCGGTTGCCGGAGGCGACGCTCGCGCGGCTTATCAGGCCTACACCGCTGGGAAGGGCTCCACCCCGTGGGGCGGATAAGGCggggacg                                                          >  1:4705391/1‑145 (MQ=14)
                                                                                              aGTGCTCCTCCCCGCAGGTCGGTGAAGGCGGTTTCGCCGCCTCCGACAGCCAACGTCGGAGCCGGGTGCCTGATCCGAGCGTGGGCCGTGACATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgc                                                         <  2:6308885/150‑1 (MQ=11)
                                                                                               gTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgcc                                                        <  1:3294838/150‑1 (MQ=255)
                                                                                                  cTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGtt                                                                                                                                            >  1:3485384/1‑63 (MQ=255)
                                                                                                  cTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCa                                                     >  2:4978164/1‑150 (MQ=255)
                                                                                                  cTCCACCCCGTAGGTTGGATAAGGCGGATACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCCGGCCGACACCGCTGGGAAGTGCTCCCCCCCGTAGGTCGGCTAAGGCGGTTACGCCGCa                                                     >  1:2617573/1‑150 (MQ=14)
                                                                                                   tCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGCCGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTGGGCTACGGCGGTTACGCCGCAt                                                    >  1:247607/1‑150 (MQ=255)
                                                                                                   tCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCAt                                                    >  2:1346704/1‑150 (MQ=37)
                                                                                                     cACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGACTTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATcc                                                  >  2:7056040/1‑150 (MQ=255)
                                                                                                         ccGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACa                                              >  2:6222760/1‑150 (MQ=255)
                                                                                                           gTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATc                                            >  2:62476/1‑150 (MQ=255)
                                                                                                                ttGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCCCTGTGACGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACg                                       >  1:2301942/1‑150 (MQ=255)
                                                                                                                 tGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGc                                      >  2:1484769/1‑150 (MQ=38)
                                                                                                                   gATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGccc                                    <  2:7141314/150‑1 (MQ=17)
                                                                                                                    aTAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTCTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTAACGCCGCATCCGACATCTAACGCCCa                                   >  1:4394096/1‑150 (MQ=25)
                                                                                                                     tAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAgg                                                                  >  2:6859724/1‑118 (MQ=37)
                                                                                                                      aaGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCg                                                                                                                                               <  1:1617873/40‑1 (MQ=255)
                                                                                                                       aGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGc                                <  2:5084497/150‑1 (MQ=34)
                                                                                                                       aGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCCCTACACACCTCAGAAGTGAGCCACCCCGGAGGTCGGAGAAGGCGGTTACGCCGCAGCCGACATCAAACGCCCTAGc                                >  2:3042407/1‑150 (MQ=11)
                                                                                                                         gCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGGGAAGTGCTCCACCCCGTAGGTCGGATCAGGCGGGTACGCCGCAGCCGACATCTAACGCCCAAGCCg                              >  2:3407787/1‑150 (MQ=255)
                                                                                                                         gCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCGGTGAAGTGCTCCACCCCGTAGGTCGGCTCAGGCGGTTACGCCGCATCCGACATCTAACGCCCCAGCCg                              >  2:5332317/1‑150 (MQ=255)
                                                                                                                             ttACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTg                          <  1:6940809/150‑1 (MQ=33)
                                                                                                                             gtACGCCGCATCCGACATCCAACGTCCGAGCCGGTAGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGAGGAGTGCTACACCCCGTTGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCACGCCGGTTg                          >  2:6683299/2‑150 (MQ=11)
                                                                                                                              caCGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGa                                                <  1:6237041/126‑1 (MQ=14)
                                                                                                                               aCGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCg                                                                <  1:2771808/110‑1 (MQ=32)
                                                                                                                                cgccgcATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCt                       <  1:5863881/150‑1 (MQ=32)
                                                                                                                                  ccgcATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGa                     >  1:2903457/1‑150 (MQ=32)
                                                                                                                                  ccgcATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGa                     >  2:339228/1‑150 (MQ=32)
                                                                                                                                  ccgcATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGa                     >  2:3251973/1‑150 (MQ=32)
                                                                                                                                  ccgcATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGGCTTATCAGGCCTACACCGCTGTGAAGTGCGCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGa                     >  1:4476132/1‑150 (MQ=17)
                                                                                                                                   cgcATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTgct                    >  1:5645599/1‑148 (MQ=17)
                                                                                                                                       tCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGTTAAGGCGGTTACGCCGCATCCGACATCTAACGCCCCAGCCCGTTGCCTGATGCgc                >  1:6177371/1‑149 (MQ=12)
                                                                                                                                         cGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGCCCCCCCGTAGGTCGGATAAGGCGGGGACGCCGCAACCGACCTCGAACGCCCAAGCCGGTTGCCTGATGCGCCg              >  1:3786634/1‑150 (MQ=11)
                                                                                                                                         cGACATCCAACGTCCGAGCCGGTTGCCTGACGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGAGAAGTGCGACACCCCGTAGGTCGGATAAGGCGGATACGCCGCAGCCGACATCTCACGCCCGAGCCGGTTGCCAGATGCGACg              >  2:3989519/1‑150 (MQ=11)
                                                                                                                                              tCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgc         >  2:3150498/1‑150 (MQ=32)
                                                                                                                                                 aaCGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCCAGCCGGTTGCCTGATGCGACGCTGGcgcg      >  2:4170651/1‑150 (MQ=17)
                                                                                                                                                 aaCGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGcgcg      <  1:759644/150‑1 (MQ=32)
                                                                                                                                                 aaCGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGcgcg      >  2:2017037/1‑150 (MQ=32)
                                                                                                                                                 aaCGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGcgcg      >  2:216989/1‑150 (MQ=32)
                                                                                                                                                  aCGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTATGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGt     <  2:3683710/150‑1 (MQ=17)
                                                                                                                                                  aCGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGt     <  1:88898/150‑1 (MQ=32)
                                                                                                                                                   cGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTc    <  1:6218078/150‑1 (MQ=32)
                                                                                                                                                   cGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTc    <  1:1933248/150‑1 (MQ=32)
                                                                                                                                                     tCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCtt  <  2:338165/150‑1 (MQ=11)
                                                                                                                                                     |                                                                                                                                                     
GGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTT  >  NC_000913/2304650‑2304947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: