Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,464,225 T→G 5.3% intergenic (+155/+27) yfdF → / ← mlaA uncharacterized protein/ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,464,2250TG 5.3% 1138.2 / 40.2 316intergenic (+155/+27)yfdF/mlaAuncharacterized protein/ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component
Reads supporting (aligned to +/- strand):  ref base T (161/138);  new base G (3/14);  total (164/152)
Fisher's exact test for biased strand distribution p-value = 4.80e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

Only 100 of 325 total aligned reads displayed.

TTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCAT  >  NC_000913/2464076‑2464374
                                                                                                                                                     |                                                                                                                                                     
tttttCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGttt                                                                                                                                                       >  1:350619/1‑150 (MQ=255)
     ccAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGa                                                                                                                                                  >  2:2773698/1‑150 (MQ=255)
      cAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGAc                                                                                                                                                 >  2:6639430/1‑150 (MQ=255)
      cAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGGGGTACTTGAGGGATAACAACAAAAACGGTGAGTGTTGCGAc                                                                                                                                                 >  2:5251719/1‑150 (MQ=255)
       tgTTTTTTTATTTTTCAACAGCTGAATATCGTAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACt                                                                                                                                                <  1:5043761/149‑1 (MQ=255)
        gTTTTTTGATTTTTCAACAGCTGAATATCGTAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTc                                                                                                                                               <  1:2484972/150‑1 (MQ=255)
          tttttGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCAc                                                                                                                                             <  1:706135/150‑1 (MQ=255)
           ttttGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCAcc                                                                                                                                            >  1:982283/1‑150 (MQ=255)
               gATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCtttt                                                                                                                                        <  1:5304639/150‑1 (MQ=255)
               gATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCtttt                                                                                                                                        >  2:6073108/1‑150 (MQ=255)
                aTTTGTCAACAGCTGACTATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTCGCAAAACTCACCttttt                                                                                                                                       <  2:5616126/150‑1 (MQ=255)
                 tttttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCtttttt                                                                                                                                      >  1:6226558/1‑150 (MQ=255)
                 tttttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTCGCAAAACTCACCtttttt                                                                                                                                      <  1:7105603/150‑1 (MQ=255)
                  ttttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTa                                                                                                                                     >  2:1768560/1‑150 (MQ=255)
                    ttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTAtt                                                                                                                                   >  2:3694759/1‑150 (MQ=255)
                     tCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTAttt                                                                                                                                  <  2:3231759/150‑1 (MQ=255)
                     tCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTCGCAAAACTCACCTTTTTTGttg                                                                                                                                  <  2:7171935/150‑2 (MQ=39)
                      cAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTCGCAAAACTCACCTTTTTTGttgt                                                                                                                                 <  1:1376355/150‑3 (MQ=38)
                       aaCAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGt                                                                                                                                <  1:5633143/150‑1 (MQ=255)
                        aCAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGtt                                                                                                                               <  1:2663219/150‑1 (MQ=255)
                        aCAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGtt                                                                                                                               <  2:3925556/150‑1 (MQ=255)
                        aCAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGtt                                                                                                                               <  1:585146/150‑1 (MQ=255)
                         cAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATCATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGttt                                                                                                                              <  2:3060653/150‑1 (MQ=255)
                         cAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGttt                                                                                                                              <  2:1270050/150‑1 (MQ=255)
                         cAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGttt                                                                                                                              <  1:2729880/150‑1 (MQ=255)
                          aGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTc                                                                                                                             >  1:911722/1‑150 (MQ=255)
                            cTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCtt                                                                                                                           <  2:4820865/150‑1 (MQ=255)
                            cTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCtt                                                                                                                           <  1:3338820/150‑1 (MQ=255)
                             tGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTa                                                                                                                          <  2:5314565/150‑1 (MQ=255)
                             tGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTa                                                                                                                          <  1:2847964/150‑1 (MQ=255)
                             tGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACGTGGGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTa                                                                                                                          >  1:2160396/1‑150 (MQ=255)
                              gAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTAt                                                                                                                         >  1:1646948/1‑150 (MQ=255)
                                 tatCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCa                                                                                                                      <  2:3804777/150‑1 (MQ=255)
                                  atCGGAAATAGTCAGTGACGAACATCAGTATTTTCACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAg                                                                                                                     <  2:2519280/150‑1 (MQ=255)
                                   tCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGTGGGATAACAACAAAATAGGTGAGTTTTGCGACTCTCGTTTTTTTTTTGTTTGTTATTCAGa                                                                                                                    >  1:4499043/1‑150 (MQ=255)
                                   tCGGAAATAGCCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGa                                                                                                                    <  1:668641/150‑1 (MQ=255)
                                     ggAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAAt                                                                                                                  >  2:1716488/1‑150 (MQ=255)
                                           aGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAatat                                                                                                            >  2:6203540/1‑150 (MQ=255)
                                             tCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCt                                                                                                          >  2:5713877/1‑150 (MQ=255)
                                               aGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCttt                                                                                                        >  2:6678150/1‑150 (MQ=255)
                                               aGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCttt                                                                                                        >  1:6741831/1‑150 (MQ=255)
                                               aGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCttt                                                                                                        >  2:3594131/1‑150 (MQ=255)
                                               aGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCttt                                                                                                        <  2:7045407/150‑1 (MQ=255)
                                                gTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCtttt                                                                                                       <  1:1932841/150‑1 (MQ=255)
                                                  gACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTaa                                                                                                     <  2:6521957/150‑1 (MQ=255)
                                                     gAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAatc                                                                                                  >  1:6039955/1‑150 (MQ=255)
                                                     gAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAatc                                                                                                  <  1:4338476/150‑1 (MQ=255)
                                                            aGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGa                                                                                           >  2:4677513/1‑150 (MQ=255)
                                                             gTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGaa                                                                                          >  2:6876840/1‑150 (MQ=255)
                                                             gTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGaa                                                                                          <  2:2964503/150‑1 (MQ=255)
                                                              tATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTTTTTCTTATTCAGAATAAATATATTTTAAATCATCCTGAAt                                                                                         >  1:4160696/1‑150 (MQ=255)
                                                                tttttACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCg                                                                                       <  1:4236929/150‑1 (MQ=255)
                                                                  tttACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTAACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCt                                                                                     >  1:1006664/1‑150 (MQ=255)
                                                                      cATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTtgtg                                                                                 >  2:347982/1‑150 (MQ=255)
                                                                       aTAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGc                                                                                <  1:4056802/150‑1 (MQ=255)
                                                                           ttACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTc                                                                            <  1:4921082/150‑1 (MQ=255)
                                                                              cGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGa                                                                         <  1:5227141/150‑1 (MQ=255)
                                                                                tCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGAtt                                                                       >  2:4042881/1‑150 (MQ=255)
                                                                                   aGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTc                                                                    <  1:4046021/150‑1 (MQ=255)
                                                                                   aGGTCATTGTCGGTTTATCTCCTTTTAATTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATACTGAATCGCGTTTGCGTTCGGATTTTc                                                                    >  1:4831075/1‑150 (MQ=255)
                                                                                   aGGTCATTGTCGGTTTATATCCTTTTACGTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTATTATTCAGAATCACTCTCTTTTAAATAATCCTGAATCGCTTGTGCGTTCGGAttttg                                                                    >  1:6943999/1‑149 (MQ=255)
                                                                                     gTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTAGTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCt                                                                  >  2:6443144/1‑150 (MQ=255)
                                                                                         ttGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCgg                                                              <  1:832253/150‑1 (MQ=255)
                                                                                             cGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTg                                                          >  2:3301830/1‑150 (MQ=255)
                                                                                              ggTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGa                                                         <  1:1160874/150‑1 (MQ=255)
                                                                                              ggTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGa                                                         <  1:6409433/150‑1 (MQ=255)
                                                                                              ggTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGa                                                         <  1:3228335/150‑1 (MQ=255)
                                                                                                 ttATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGtt                                                      >  2:5136297/1‑150 (MQ=255)
                                                                                                   aTCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTcg                                                    >  1:1556700/1‑150 (MQ=255)
                                                                                                   aTCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTcg                                                    >  2:1116431/1‑150 (MQ=255)
                                                                                                     ctcCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTcgcc                                                  >  1:4406183/1‑150 (MQ=255)
                                                                                                       ccTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTcgccgc                                                <  1:4318500/150‑1 (MQ=255)
                                                                                                          tttACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCAt                                             >  1:2574541/1‑150 (MQ=255)
                                                                                                              cTTGTGGTAATTGAGGGATAACAACAAACAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGc                                         <  2:5850713/150‑1 (MQ=255)
                                                                                                                   ggTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGa                                    >  1:3074465/1‑150 (MQ=255)
                                                                                                                   ggTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTGTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGGTTGAGTTCGCCGCCATTAGCGATGa                                    >  2:4286191/1‑150 (MQ=255)
                                                                                                                   ggTAATTGAGGGAAAACAACAAAAAAGGTGAGTTTTGCGACTCACCTATTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAAACATACTGAATCGCTAGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGa                                    >  1:1987607/1‑150 (MQ=255)
                                                                                                                    gTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGaa                                   <  2:2364993/150‑1 (MQ=255)
                                                                                                                        ttGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCa                               <  1:2773698/150‑1 (MQ=255)
                                                                                                                          gAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATg                             >  2:500964/1‑150 (MQ=255)
                                                                                                                              gATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGc                         >  2:4548734/1‑150 (MQ=255)
                                                                                                                               aTAACAACAAAAAAGGTGAGTTTTGCGAATCACCTTTTTTATTTGTTTCTTATTCAGATTCTATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGTTTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCt                        >  2:596514/1‑150 (MQ=255)
                                                                                                                                tAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTg                       >  2:2116806/1‑150 (MQ=255)
                                                                                                                                tAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTg                       <  2:1858061/150‑1 (MQ=255)
                                                                                                                                 acaaataaaaaaGGTGAGTCGCAAAACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCCTTAGCGATGAAATCATGACGCTgg                      >  2:410920/7‑150 (MQ=38)
                                                                                                                                 acaaataaaaaaGGTGAGTCGCAAAACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTgg                      >  2:1838205/7‑150 (MQ=38)
                                                                                                                                 acaaataaaaaaGGTGAGTCGCAAAACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCGTGTGCGTTCGGATTTTCCTGCGGTGTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTgg                      >  2:5554487/7‑150 (MQ=38)
                                                                                                                                    aacaaAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAg                   >  2:3461167/1‑150 (MQ=255)
                                                                                                                                    aacaaAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAg                   >  1:6486449/1‑150 (MQ=255)
                                                                                                                                    aacaaAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAg                   >  2:1559994/1‑150 (MQ=255)
                                                                                                                                    aacaaAAAAGGTGAGTTTTGCGACTCACCTATTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCGTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGTTGAAATCATGACGATGGacg                   >  1:3205736/1‑148 (MQ=255)
                                                                                                                                       aaaaaaGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTAc                >  2:2566709/1‑150 (MQ=255)
                                                                                                                                         aaaaGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGc              >  2:5518635/1‑150 (MQ=255)
                                                                                                                                          aaaGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCt             <  1:3828343/150‑1 (MQ=255)
                                                                                                                                          aaaGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCt             <  1:1854280/150‑1 (MQ=255)
                                                                                                                                             ggTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTcg          <  2:4263560/150‑1 (MQ=255)
                                                                                                                                               tGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTcgcg        >  1:7166635/1‑150 (MQ=255)
                                                                                                                                                   ttttGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGTAATCATGACGCTGGAAGTACGCTTCGCGCAcc    >  2:4383203/1‑150 (MQ=255)
                                                                                                                                                   ttttGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCAcc    >  2:6395674/1‑150 (MQ=255)
                                                                                                                                                     ttGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCat  >  2:5330526/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCAT  >  NC_000913/2464076‑2464374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: