Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,464,226 T→C 5.1% intergenic (+156/+26) yfdF → / ← mlaA uncharacterized protein/ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,464,2260TC 5.1% 1082.3 / 29.8 316intergenic (+156/+26)yfdF/mlaAuncharacterized protein/ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component
Reads supporting (aligned to +/- strand):  ref base T (160/139);  new base C (3/13);  total (164/152)
Fisher's exact test for biased strand distribution p-value = 8.73e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01

Only 100 of 330 total aligned reads displayed.

TTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCATA  >  NC_000913/2464077‑2464375
                                                                                                                                                     |                                                                                                                                                     
    ccAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGa                                                                                                                                                   <  2:1823192/150‑1 (MQ=255)
     cAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGAc                                                                                                                                                  >  2:6639430/1‑150 (MQ=255)
     cAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGAc                                                                                                                                                  >  2:4649699/1‑150 (MQ=255)
      aGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACt                                                                                                                                                 <  2:1228831/150‑1 (MQ=255)
       gTTTTTTGATTTTTCAACAGCTGAATATCGTAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTc                                                                                                                                                <  1:2484972/150‑1 (MQ=255)
              gATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCtttt                                                                                                                                         >  2:6073108/1‑150 (MQ=255)
              gATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCtttt                                                                                                                                         <  1:5304639/150‑1 (MQ=255)
              gATTTTTCAACAGCTGAATATCGGAAATAGTCAGGGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCtttt                                                                                                                                         <  2:5329174/150‑1 (MQ=255)
               aTTTTTCCACAGCTGAAGATCGGAAATAGTCAGTGACGAACATCAGTATTTGTCCATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTCGCAAAACTCACCttttt                                                                                                                                        <  2:3814014/150‑1 (MQ=255)
               aTTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACCTCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTCGCAAAACTCACCttttt                                                                                                                                        <  2:5616107/150‑1 (MQ=255)
                tttttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTCGCAAAACTCACCtttttt                                                                                                                                       <  1:6950877/150‑1 (MQ=255)
                tttttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTCGCAAAACTCACCtttttt                                                                                                                                       <  1:3076815/150‑1 (MQ=255)
                 ttttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTa                                                                                                                                      >  2:2698176/1‑150 (MQ=255)
                 ttttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTa                                                                                                                                      >  2:1540354/1‑150 (MQ=255)
                 ttttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTa                                                                                                                                      >  2:6290945/1‑150 (MQ=255)
                  tttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTAt                                                                                                                                     >  1:5868249/1‑150 (MQ=255)
                   ttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTCGCAAAACTCACCTTTTTTGtt                                                                                                                                    <  1:4410861/150‑1 (MQ=255)
                    tCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTAttt                                                                                                                                   >  2:511589/1‑150 (MQ=255)
                    tCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTCGCAAAACTCACCTTTTTTGttg                                                                                                                                   <  2:7171935/150‑2 (MQ=39)
                    tCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGAATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTAttt                                                                                                                                   >  2:172534/1‑150 (MQ=255)
                     cAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTCGCAAAACTCACCTTTTTTGttgt                                                                                                                                  <  1:1376355/150‑3 (MQ=38)
                      caCAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGt                                                                                                                                 <  2:4752209/149‑1 (MQ=255)
                      aaCAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGt                                                                                                                                 >  1:6456282/1‑150 (MQ=255)
                      aaCAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGt                                                                                                                                 <  1:5633143/150‑1 (MQ=255)
                        cAGCTGAATCTCGGAAATAGTCAGTGCCGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGttt                                                                                                                               <  2:6676511/150‑1 (MQ=255)
                        cAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGttt                                                                                                                               <  1:2729880/150‑1 (MQ=255)
                        cAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGttt                                                                                                                               <  1:2660864/150‑1 (MQ=255)
                        cAGCTGAATAACGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAAGAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGttt                                                                                                                               <  2:5545210/150‑1 (MQ=255)
                           cTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCtt                                                                                                                            <  1:3338820/150‑1 (MQ=255)
                            tGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACGTGGGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTa                                                                                                                           >  1:2160396/1‑150 (MQ=255)
                             gAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTAt                                                                                                                          >  1:1646948/1‑150 (MQ=255)
                              aaTATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTAtt                                                                                                                         <  1:892141/150‑1 (MQ=255)
                               atatCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTc                                                                                                                        <  2:2077363/150‑1 (MQ=255)
                                tatCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAAGTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCa                                                                                                                       <  2:4363108/150‑1 (MQ=255)
                                 atCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCCTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAg                                                                                                                      <  1:2900768/150‑1 (MQ=255)
                                  tCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGTGGGATAACAACAAAATAGGTGAGTTTTGCGACTCTCGTTTTTTTTTTGTTTGTTATTCAGa                                                                                                                     >  1:4499043/1‑150 (MQ=255)
                                  tCGGAAATAGCCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGa                                                                                                                     <  1:668686/150‑1 (MQ=255)
                                   cGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAAGTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGaa                                                                                                                    <  2:3096711/150‑1 (MQ=255)
                                    ggAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAAt                                                                                                                   >  2:6418034/1‑150 (MQ=255)
                                    ggAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTAAGAAt                                                                                                                   >  1:5062123/1‑150 (MQ=255)
                                      aaaTAGACAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGACATTGTCGGTTTATCTCCATTTACTTTTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTTCGACTCACCAATTTTAATTGTTTCTTATTCAGAATCa                                                                                                                 >  1:7224216/1‑150 (MQ=255)
                                        aTAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAAGTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTGTTGTATTTGTTTCTTATTCAGAATCAat                                                                                                               >  2:4085642/1‑150 (MQ=255)
                                          aGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAatat                                                                                                             >  2:6203540/1‑150 (MQ=255)
                                            tCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCt                                                                                                           >  2:5713877/1‑150 (MQ=255)
                                              aGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCttt                                                                                                         <  2:7045407/150‑1 (MQ=255)
                                              aGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACGTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTGTTTTATTTGTTTCTTATTCAGAATCAATCTCttt                                                                                                         >  2:185361/1‑150 (MQ=255)
                                               gTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCtttt                                                                                                        <  1:1932841/150‑1 (MQ=255)
                                                 gACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTaa                                                                                                      <  2:6521957/150‑1 (MQ=255)
                                                  aCGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAATAAAGGTGAGTTTTGCGACTCTCCTTTTTTATTTGTTTCTTATTCAGATTCAATATGTTTTaaa                                                                                                     >  1:3530313/1‑150 (MQ=255)
                                                      aCATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAatcat                                                                                                 <  1:6344147/150‑1 (MQ=255)
                                                       cATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAatcatc                                                                                                <  2:3557128/150‑1 (MQ=255)
                                                       cATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAatcatc                                                                                                <  2:336199/150‑1 (MQ=255)
                                                       aaTCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAatcatc                                                                                                <  2:5165212/149‑1 (MQ=255)
                                                        aTCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTCTCTCCTTTTTCTTGTGGTAATTGAGGGATTACAACAAAAAAGGTGCGTTTTGCGACTCAGCTTTTTTATTTGTGTCTGATTCAGTATCAATCTCTTGTTAATCATcc                                                                                               >  2:1024647/1‑150 (MQ=255)
                                                            gTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGaa                                                                                           >  2:2879530/1‑150 (MQ=255)
                                                            gTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGaa                                                                                           >  2:6876840/1‑150 (MQ=255)
                                                             tATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTTCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCAGAAt                                                                                          >  1:4161597/1‑150 (MQ=255)
                                                             tATTTTTACATAATAACGTCTAGGTCATTGTCGGTTTATCTCCATTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTAACCTTTTTTATTTTTTTCTTATTCAGAATAAATATCTTTTAAATCATCATGAAt                                                                                          >  1:6770167/1‑150 (MQ=255)
                                                               tttttACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCg                                                                                        <  2:5286768/150‑1 (MQ=255)
                                                                 tttACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTAACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCt                                                                                      >  1:1006664/1‑150 (MQ=255)
                                                                   tACATAATTACGTCTAGGTCATTGTCGGTTTTTCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTtg                                                                                    <  1:4384062/150‑1 (MQ=255)
                                                                    aCATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTTCGACTCACCTTTTTTATTTGTTTATTATTCAGAATAAATATATTTTAAATCATCCTGAATCGCTtgt                                                                                   >  1:2726480/1‑150 (MQ=255)
                                                                          ttACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTTGGAATTGAGGGATAACAACAAAAAAGGTGAGGTTTGCGACTCACATTTTTTATTTGTTTCTGATGCATAATCAATATATTTTAAATCATCCTGAATAGATTGTGCGTTc                                                                             >  2:232538/1‑150 (MQ=255)
                                                                          ttACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTc                                                                             <  2:3954007/150‑1 (MQ=255)
                                                                          ttACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTc                                                                             <  1:211868/150‑1 (MQ=255)
                                                                             cGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGa                                                                          <  1:5227141/150‑1 (MQ=255)
                                                                                 tAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGAtttt                                                                      >  2:4145119/1‑150 (MQ=255)
                                                                                  aGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTc                                                                     <  1:4046037/150‑1 (MQ=255)
                                                                                  aGGTCATTGTCGGTTTATCTCCTTTTAATTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATACTGAATCGCGTTTGCGTTCGGATTTTc                                                                     >  1:4831075/1‑150 (MQ=255)
                                                                                      cATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGc                                                                 >  2:4497844/1‑150 (MQ=255)
                                                                                          gTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGtt                                                             >  2:3284929/1‑150 (MQ=255)
                                                                                           tCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGttt                                                            >  2:4951351/1‑150 (MQ=255)
                                                                                             ggTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGa                                                          <  1:1160874/150‑1 (MQ=255)
                                                                                             ggTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGa                                                          <  1:3228335/150‑1 (MQ=255)
                                                                                                  aTCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTcg                                                     >  1:1556700/1‑150 (MQ=255)
                                                                                                      ccTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTcgccgc                                                 <  1:4318514/150‑1 (MQ=255)
                                                                                                       cTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCgccgcc                                                >  1:7107906/1‑150 (MQ=255)
                                                                                                             cTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGc                                          <  2:5850694/150‑1 (MQ=255)
                                                                                                             cTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGc                                          <  2:2501132/150‑1 (MQ=255)
                                                                                                              ttGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCg                                         <  1:3194226/150‑1 (MQ=255)
                                                                                                              ttGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCg                                         >  2:3667096/1‑150 (MQ=255)
                                                                                                               tgtgGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGa                                        <  2:5457229/150‑1 (MQ=255)
                                                                                                                  ggTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGATCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGa                                     >  2:4286110/1‑150 (MQ=255)
                                                                                                                      aTTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATc                                 <  1:426046/150‑1 (MQ=255)
                                                                                                                       ttGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCa                                <  1:2773698/150‑1 (MQ=255)
                                                                                                                       ttGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCa                                <  1:4091615/150‑1 (MQ=255)
                                                                                                                        tGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCAt                               >  1:945456/1‑150 (MQ=255)
                                                                                                                        tGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGGTTGAGTTCGCCGCCATTAGCGATGAAATCAt                               >  1:6103801/1‑150 (MQ=255)
                                                                                                                         gAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATg                              >  2:5890825/1‑150 (MQ=255)
                                                                                                                          aGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCAGCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGa                             >  1:4985817/1‑150 (MQ=255)
                                                                                                                            ggATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACg                           >  1:7073200/1‑150 (MQ=255)
                                                                                                                                  caacaaAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGaa                     <  1:6617334/150‑1 (MQ=255)
                                                                                                                                  caacaaAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAAGATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGaa                     >  2:1680290/1‑150 (MQ=255)
                                                                                                                                   aacaaAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAg                    >  2:369727/1‑150 (MQ=255)
                                                                                                                                   aacaaAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAg                    >  2:3461229/1‑150 (MQ=255)
                                                                                                                                      aaaaaaGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTAc                 >  2:2965188/1‑150 (MQ=255)
                                                                                                                                         aaaGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCt              <  1:3828344/150‑1 (MQ=255)
                                                                                                                                         aaaGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCt              <  1:1854280/150‑1 (MQ=255)
                                                                                                                                                  ttttGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCAcc     >  2:2519993/1‑150 (MQ=255)
                                                                                                                                                     tGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCata  <  1:5815666/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCATA  >  NC_000913/2464077‑2464375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: