Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,521,344 C→T 6.0% intergenic (+16/+249) lysV → / ← xapR tRNA‑Lys/transcriptional activator of xapAB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,521,3440CT 6.0% 807.5 / 29.5 282intergenic (+16/+249)lysV/xapRtRNA‑Lys/transcriptional activator of xapAB
Reads supporting (aligned to +/- strand):  ref base C (164/99);  new base T (8/9);  total (172/110)
Fisher's exact test for biased strand distribution p-value = 3.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 287 total aligned reads displayed.

CCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAA  >  NC_000913/2521199‑2521493
                                                                                                                                                 |                                                                                                                                                     
ccTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTaaa                                                                                                                                                   >  1:4129116/1‑150 (MQ=255)
 cTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAg                                                                                                                                                  >  1:7133718/1‑150 (MQ=255)
 cTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAg                                                                                                                                                  >  1:2540270/1‑150 (MQ=255)
  tCCCTTACACGGTGGGGGTCGGCGGTTCTATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGTTAGAGCAGTTTACTTTTAATCATTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAgg                                                                                                                                                 <  1:63395/150‑1 (MQ=17)
  tCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAgg                                                                                                                                                 >  1:6081981/1‑150 (MQ=255)
    ccTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgcg                                                                                                                                               >  1:3032835/1‑150 (MQ=255)
        aCAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGCGTCGTTAGCTCAGTTGCTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCTTTAGGGCGCttt                                                                                                                                           <  2:390884/150‑1 (MQ=17)
         cAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCTTTAGGGCGCtttt                                                                                                                                          <  2:5043110/150‑1 (MQ=255)
          aaGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCTTTAGGGCGCttttt                                                                                                                                         <  2:4777644/150‑1 (MQ=255)
          aaGGAGGGGGTCGGCGGTTCGATCCCGTAATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCatttt                                                                                                                                         <  2:4269482/150‑1 (MQ=255)
           aggaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCTTTAGGGCGCtttttt                                                                                                                                        <  2:5583639/150‑1 (MQ=255)
            ggaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCCCCAAGGTAAAAAAGCGCCCGAAAGGCGCTTTTTTa                                                                                                                                       >  1:469232/1‑150 (MQ=255)
              aggGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACt                                                                                                                                     <  1:5223200/150‑1 (MQ=255)
                  ggTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCt                                                                                                                                 >  2:4890732/1‑150 (MQ=255)
                    tCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTTCTATCTGc                                                                                                                               >  1:7169968/1‑150 (MQ=255)
                    tCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGc                                                                                                                               <  2:3708392/150‑1 (MQ=255)
                      ggcggTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGa                                                                                                                             <  1:5391073/150‑1 (MQ=255)
                       gcggTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGAt                                                                                                                            >  1:6831207/1‑150 (MQ=255)
                           ttCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGATGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCGAAAGGCGCTTTTTTACTATCTGCGATAcac                                                                                                                        >  1:4474244/1‑148 (MQ=255)
                             cGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCaa                                                                                                                      <  1:5976094/150‑1 (MQ=255)
                              gATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTTCTATCTGCGATACTCaaa                                                                                                                     <  1:1854420/150‑1 (MQ=255)
                              gATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCaaa                                                                                                                     <  1:269031/150‑1 (MQ=255)
                              gATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATATTCGATACTCaaa                                                                                                                     >  1:1549570/1‑150 (MQ=255)
                              gATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTACACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCaaa                                                                                                                     <  1:5623425/150‑1 (MQ=255)
                               aTCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGGACGTCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTTCTATCTGCGATACTCAAAg                                                                                                                    >  1:4361297/1‑150 (MQ=255)
                               aTCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAAAGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAg                                                                                                                    >  1:817925/1‑150 (MQ=255)
                                tCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGa                                                                                                                   >  1:1047182/1‑150 (MQ=255)
                                 cccGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGAt                                                                                                                  <  1:1743820/150‑1 (MQ=255)
                                  ccGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGAtt                                                                                                                 <  1:6953637/150‑1 (MQ=255)
                                  ccGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGAtt                                                                                                                 <  1:3949156/150‑1 (MQ=255)
                                  ccGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACACACCAATGTAAAAAAGCGCCCTAAAGGCGCATTTTTACTATCTGCGATAAGCGAAGAtt                                                                                                                 >  2:4413963/1‑150 (MQ=17)
                                   cgacatcaCCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAACGCGCTTTTTTACTATCTGCGATACTCAAAGATTc                                                                                                                <  2:275848/147‑1 (MQ=255)
                                   cGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTc                                                                                                                <  1:2468015/150‑1 (MQ=255)
                                    gTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTTGTAGAGCATTTTACTTTTAATAAATTTGTGGCAGGTTCGAATCCTTCACGACCCACCAATGTAAATAATAGCACTAAAGGCGCTTTTTTTATATATGCGATAATCAAATAttag                                                                                                               >  1:1373142/1‑148 (MQ=17)
                                    gTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCg                                                                                                               >  2:850234/1‑150 (MQ=255)
                                     tcatcaCCCACCACTTCGGGTCGTTAGCTCAGGTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGa                                                                                                              >  1:164970/1‑150 (MQ=255)
                                      catcaCCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAAAGTAAAAAAGCGCCCGAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGaa                                                                                                             >  1:3322139/1‑150 (MQ=255)
                                        tcaCCCACCACTTCGGGTCGTTAGCTCAGGTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAAcc                                                                                                           >  2:3574607/1‑150 (MQ=255)
                                          acccaccACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTg                                                                                                         <  1:5752408/150‑1 (MQ=255)
                                          acccaccACTTCGGGTCGTGAGCTCAGGGGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTg                                                                                                         <  1:781836/150‑1 (MQ=255)
                                           cccaccACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTgc                                                                                                        <  2:7038662/150‑1 (MQ=40)
                                            ccaccaCTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTgca                                                                                                       >  2:2893605/1‑150 (MQ=40)
                                             caccacTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTgccg                                                                                                      >  2:2442788/1‑148 (MQ=39)
                                               ccacTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTACAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTgcagca                                                                                                    <  1:627386/150‑1 (MQ=38)
                                                 acTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCTATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAgg                                                                                                  <  1:3669342/150‑1 (MQ=25)
                                                 acTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAgg                                                                                                  <  1:3011479/150‑1 (MQ=35)
                                                       ggTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgag                                                                                            <  2:3811810/150‑1 (MQ=32)
                                                          cGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagttg                                                                                         <  1:955864/150‑1 (MQ=32)
                                                           gTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagttga                                                                                        <  1:5879443/150‑1 (MQ=32)
                                                            ttAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagttgag                                                                                       >  1:3243497/1‑150 (MQ=32)
                                                            ttAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagttgag                                                                                       >  1:6595785/1‑150 (MQ=32)
                                                              aGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAAACTGCAGCAGGTTTGAGTTGAgcg                                                                                     >  2:4122819/1‑150 (MQ=17)
                                                                cTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCa                                                                                   <  2:6427344/150‑1 (MQ=32)
                                                                cTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCa                                                                                   <  1:2379530/150‑1 (MQ=32)
                                                                        gTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAaaca                                                                           >  2:3245310/1‑150 (MQ=32)
                                                                           gagCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCATGTTTGAGTTGAGCGCAGCGAAACAACg                                                                        >  2:1557426/1‑150 (MQ=17)
                                                                           gagCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACg                                                                        <  2:1829584/150‑1 (MQ=32)
                                                                            agCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAATATTCGAACCTGCCGCCGGTTTGAGTTGAGCGCAGCGACACAACgg                                                                       >  1:5667775/1‑150 (MQ=255)
                                                                            agCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGATCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAATCAACgg                                                                       >  2:6329649/1‑150 (MQ=17)
                                                                             gCAGTTGAATTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAACGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGTGTTGAGCGCAGCGAAACAACGGa                                                                      <  2:6915970/150‑1 (MQ=12)
                                                                              cAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAg                                                                     >  1:4991047/1‑150 (MQ=32)
                                                                                gTTGACTTTTACTCATTTGGTCGCTGGTTCGAAGCCGGCACGCCCCACCACGGTAAACAAGCGCCCTACTGGGGCTTTTTTACTATCTGCGATACTCAAAGAGTCGCACCTGCTGCAGGTTTGAGTTGCGGGCCGCGAAACAACGGCGcc                                                                   <  2:774512/150‑1 (MQ=11)
                                                                                      ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGAAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTcgc                                                             >  2:7173452/1‑150 (MQ=17)
                                                                                      ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTCCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTcgc                                                             >  1:4377644/1‑150 (MQ=17)
                                                                                       tttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTAGAGTTGAGCGCAGCGAAACAACGGAGCCGCAcgcg                                                            >  1:4562210/1‑150 (MQ=17)
                                                                                        ttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCCAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcgg                                                           >  2:6486467/1‑150 (MQ=17)
                                                                                         taataaATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcggc                                                          <  2:314883/150‑1 (MQ=17)
                                                                                         tAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcggc                                                          <  2:3992265/150‑1 (MQ=32)
                                                                                          aaTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCCAAGATTCGAACCTGCAGCAGGTTAGAGTTGAGCGCAGCGAAACAAAGGAGCCGCTCgcggcg                                                         >  1:4871082/1‑150 (MQ=12)
                                                                                            tCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGAc                                                       >  1:6449754/1‑150 (MQ=32)
                                                                                                ttGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGcc                                                   >  1:1246937/1‑150 (MQ=32)
                                                                                                    tCGCAGGTTCGAATCCTGCACGACCCACAAATTTAAAAAAGCGCCCTAACGGCGATTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGTTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGaa                                               <  2:1513081/150‑1 (MQ=11)
                                                                                                     cGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAg                                              <  2:474954/150‑1 (MQ=32)
                                                                                                          gTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGgcga                                         >  1:1423380/1‑150 (MQ=32)
                                                                                                             cGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCCAAGATTCGATCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGCCGACGGCCCGAAGGgcgagcg                                      >  2:6956686/1‑150 (MQ=12)
                                                                                                             cGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGgcgagcg                                      <  2:4030698/150‑1 (MQ=32)
                                                                                                                 tCCGGCACGACCCACCAATGTAAAAAAGCGCCCCAACGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagc                                  <  2:126943/150‑1 (MQ=12)
                                                                                                                    tGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTAGAGTTGAGCGCATAGAAACAACTCAGCCGCACGCCGCGACGGCCCGAAGGGCGAGCGAAGCGAg                               >  1:4089572/1‑150 (MQ=11)
                                                                                                                      cACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTc                             >  1:2857863/1‑150 (MQ=32)
                                                                                                                      cACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTc                             >  2:5339607/1‑150 (MQ=32)
                                                                                                                      cACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGGTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTc                             >  2:5866175/1‑150 (MQ=17)
                                                                                                                      cACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACATGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTc                             >  2:2143291/1‑150 (MQ=17)
                                                                                                                         gACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGACACCACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCagc                          >  2:6086528/1‑148 (MQ=12)
                                                                                                                             caccaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGTACCTGCAGCAGGTTTGAGTTGAGCGCAGCGACACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGc                      >  1:3451576/1‑150 (MQ=17)
                                                                                                                              cagatagTAAAAAAGCGCCTTTAGGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGGTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCa                     >  2:3177960/7‑150 (MQ=11)
                                                                                                                              accaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCa                     >  2:2565127/1‑150 (MQ=32)
                                                                                                                                caATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACg                   >  2:1041371/1‑150 (MQ=32)
                                                                                                                                 aaTGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGa                  <  2:2320327/150‑1 (MQ=32)
                                                                                                                                     tAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGACGGGCGAGCGAAGCGAGTCATCCTGCACGACcca              >  2:1991424/1‑150 (MQ=17)
                                                                                                                                      aaaaaaGCGCCTTTAGGGCGCTTTTTTACTATCTGCGATACTCCAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccac             >  2:6696000/1‑150 (MQ=255)
                                                                                                                                      aaaaaaGCGCCTTTAGGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccac             >  2:4466902/1‑150 (MQ=12)
                                                                                                                                      aaaaaaGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccac             >  2:5154025/1‑150 (MQ=32)
                                                                                                                                       aaaaaGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacc            >  2:4567341/1‑150 (MQ=32)
                                                                                                                                       aaaaaGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCGGCCGCCGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacc            >  2:6985101/1‑150 (MQ=12)
                                                                                                                                       aaaaaGCCCCATAACGGCGCTTTTTTACTATCTGCTATACTCAAAGATTCTAACCTGCAGCTGGTTTTAGTTGAGCGCAGCTAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacc            <  1:5092246/150‑1 (MQ=11)
                                                                                                                                             cgcCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTa      >  2:1658187/1‑150 (MQ=32)
                                                                                                                                                ccTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTaaaa   >  2:2681644/1‑150 (MQ=32)
                                                                                                                                                ccTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTaaaa   >  2:4185472/1‑150 (MQ=32)
                                                                                                                                                 cTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTaaaaa  >  2:6058282/1‑150 (MQ=32)
                                                                                                                                                 cTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTaaaaa  >  2:6638539/1‑150 (MQ=32)
                                                                                                                                                 |                                                                                                                                                     
CCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAA  >  NC_000913/2521199‑2521493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: