Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,521,348 A→G 6.4% intergenic (+20/+245) lysV → / ← xapR tRNA‑Lys/transcriptional activator of xapAB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,521,3480AG 6.4% 760.9 / 31.5 283intergenic (+20/+245)lysV/xapRtRNA‑Lys/transcriptional activator of xapAB
Reads supporting (aligned to +/- strand):  ref base A (166/86);  new base G (8/10);  total (174/109)
Fisher's exact test for biased strand distribution p-value = 7.75e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 292 total aligned reads displayed.

CCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCG  >  NC_000913/2521199‑2521497
                                                                                                                                                     |                                                                                                                                                     
ccTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTaaa                                                                                                                                                       >  1:4129116/1‑150 (MQ=255)
 cTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAg                                                                                                                                                      >  2:3011479/1‑150 (MQ=255)
  tCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAgg                                                                                                                                                     >  1:6427344/1‑150 (MQ=255)
    ccTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgcg                                                                                                                                                   >  2:5513408/1‑150 (MQ=255)
    ccTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgcg                                                                                                                                                   >  1:3032835/1‑150 (MQ=255)
    ccTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgcg                                                                                                                                                   >  2:4872825/1‑150 (MQ=255)
    ccTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCGAAAGgcg                                                                                                                                                   >  1:6914322/1‑150 (MQ=255)
          aaGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCttttt                                                                                                                                             >  1:5921400/1‑150 (MQ=255)
          aaGGAGGGGGTCGGCGGTTCGATCCCGTAATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCatttt                                                                                                                                             <  2:4269482/150‑1 (MQ=255)
            ggtgggggTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTTACTTTTAATCATTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTa                                                                                                                                           <  1:5115275/147‑1 (MQ=255)
            ggaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCCCCAAGGTAAAAAAGCGCCCGAAAGGCGCTTTTTTa                                                                                                                                           >  1:469232/1‑150 (MQ=255)
            ggaggGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTa                                                                                                                                           >  1:3345316/1‑150 (MQ=255)
               gggggTGGGCGGTACGATCCCGTCATAACCCACCAGTTCGGTTCTTTAGCTCATTTGGTAGAGCTGTTGACTTTTAATAAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAAAAGCGCCTTTAGGGCGCTTTTTTAcat                                                                                                                                        <  1:3803224/150‑3 (MQ=17)
                ggggTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGGTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTAt                                                                                                                                       >  2:3754011/1‑150 (MQ=255)
                      ggcggTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGa                                                                                                                                 >  2:1410251/1‑150 (MQ=255)
                           ttCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCGATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTc                                                                                                                            >  2:5007057/1‑150 (MQ=255)
                           ttCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTc                                                                                                                            >  1:754069/1‑150 (MQ=255)
                              gATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTTCTATCTGCGATACTCaaa                                                                                                                         <  1:1854420/150‑1 (MQ=255)
                              gATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATATTCGATACTCaaa                                                                                                                         >  1:1549570/1‑150 (MQ=255)
                               aTCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGGACGTCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTTCTATCTGCGATACTCAAAg                                                                                                                        >  1:4361297/1‑150 (MQ=255)
                               aTCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAAAGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAg                                                                                                                        >  1:817925/1‑150 (MQ=255)
                                tCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGa                                                                                                                       >  1:1047182/1‑150 (MQ=255)
                                 cccGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGAt                                                                                                                      <  1:1743820/150‑1 (MQ=255)
                                  ccGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGAtt                                                                                                                     >  2:1633869/1‑150 (MQ=255)
                                  ccGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGAtt                                                                                                                     <  1:5357620/150‑1 (MQ=255)
                                  ccGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACACACCAATGTAAAAAAGCGCCCTAAAGGCGCATTTTTACTATCTGCGATAAGCGAAGAtt                                                                                                                     >  2:4413963/1‑150 (MQ=17)
                                   cgacatcaCCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAACGCGCTTTTTTACTATCTGCGATACTCAAAGATTc                                                                                                                    <  2:275848/147‑1 (MQ=255)
                                    gTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTTGTAGAGCATTTTACTTTTAATAAATTTGTGGCAGGTTCGAATCCTTCACGACCCACCAATGTAAATAATAGCACTAAAGGCGCTTTTTTTATATATGCGATAATCAAATAttag                                                                                                                   >  1:1373142/1‑148 (MQ=17)
                                    gTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCg                                                                                                                   >  2:850234/1‑150 (MQ=255)
                                    gTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCg                                                                                                                   <  1:6542712/150‑1 (MQ=255)
                                     tcatcaCCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGa                                                                                                                  <  1:5024878/150‑1 (MQ=255)
                                     tcatcaCCCACCACTTCGGGTCGTTAGCTCAGGTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGa                                                                                                                  >  1:164970/1‑150 (MQ=255)
                                          acccaccACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTTCTATCTGCGATAATCGAAGATTCGagcatg                                                                                                             >  1:4610211/1‑145 (MQ=255)
                                          acccaccACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTTCAATCTGCGATACTCAAAGATTCGaaaatg                                                                                                             >  1:584485/1‑146 (MQ=255)
                                           cccaccACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAAAATCGCCCGAAATGCGCTTTTGTTCTATCTGCGCTACTCAAAGATTCGAACCTgc                                                                                                            >  1:5522289/1‑150 (MQ=11)
                                           cccaccACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTgc                                                                                                            <  2:7038662/150‑1 (MQ=40)
                                             caccacTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAGCCTgcag                                                                                                          >  2:4442889/1‑150 (MQ=255)
                                             caccacTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTgccg                                                                                                          >  2:2442788/1‑148 (MQ=39)
                                                cacTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGcagcag                                                                                                       <  1:6130557/150‑1 (MQ=37)
                                                 acTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAgg                                                                                                      <  1:3011479/150‑1 (MQ=35)
                                                 acTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAgg                                                                                                      <  2:6447075/150‑1 (MQ=35)
                                                  cgtCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGt                                                                                                     <  2:667882/148‑1 (MQ=18)
                                                  cTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTCCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGt                                                                                                     >  2:3555380/1‑150 (MQ=17)
                                                          cGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagttg                                                                                             <  1:955864/150‑1 (MQ=32)
                                                            ttAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagttgag                                                                                           >  2:6422073/1‑150 (MQ=32)
                                                            ttAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagttgag                                                                                           >  1:3243497/1‑150 (MQ=32)
                                                            ttAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATAATCAAAGATTCGAACCTGCAGCAGGTttgagttgtg                                                                                           >  1:6821249/1‑148 (MQ=17)
                                                              aGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAgcg                                                                                         >  2:6545838/1‑150 (MQ=32)
                                                              aGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAAACTGCAGCAGGTTTGAGTTGAgcg                                                                                         >  2:4122819/1‑150 (MQ=17)
                                                                 tCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAg                                                                                      <  1:5636201/150‑1 (MQ=32)
                                                                        gTAGAGCAGTTGACTTTTAATCAATTTGTCGCAGGTTCGAATACAGCACGACCCACCAATGTAAAAAATCGCCCTAAAGGCGCTTTTTTAATATCTGCGATAATCAAAAATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCATCGAaaca                                                                               >  1:2453790/1‑150 (MQ=11)
                                                                           gagCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACg                                                                            <  2:1829584/150‑1 (MQ=32)
                                                                            agCAGTTGACTTTTGATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACgg                                                                           >  2:6329576/1‑150 (MQ=17)
                                                                             gCAGTTGAATTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAACGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGTGTTGAGCGCAGCGAAACAACGGa                                                                          <  2:6915970/150‑1 (MQ=12)
                                                                                gTTGACTTTTACTCATTTGGTCGCTGGTTCGAAGCCGGCACGCCCCACCACGGTAAACAAGCGCCCTACTGGGGCTTTTTTACTATCTGCGATACTCAAAGAGTCGCACCTGCTGCAGGTTTGAGTTGCGGGCCGCGAAACAACGGCGcc                                                                       <  2:774512/150‑1 (MQ=11)
                                                                                      ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGAAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTcgc                                                                 >  2:7173452/1‑150 (MQ=17)
                                                                                      ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTCCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTcgc                                                                 >  1:4377644/1‑150 (MQ=17)
                                                                                       tttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTcgcg                                                                >  1:6276316/1‑150 (MQ=32)
                                                                                         taataaATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcggc                                                              <  2:314883/150‑1 (MQ=17)
                                                                                         tAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcggc                                                              <  2:3992265/150‑1 (MQ=32)
                                                                                         tAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcggc                                                              <  2:5272004/150‑1 (MQ=32)
                                                                                                ttGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGcc                                                       >  1:1246937/1‑150 (MQ=32)
                                                                                                    tCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCCGAGATTCGAACCGGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGac                                                   >  2:3870266/1‑149 (MQ=12)
                                                                                                       cAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAggg                                                >  1:6040499/1‑150 (MQ=32)
                                                                                                          gTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGgcga                                             >  1:1423380/1‑150 (MQ=32)
                                                                                                             cGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCCAAGATTCGATCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGCCGACGGCCCGAAGGgcgagcg                                          >  2:6956686/1‑150 (MQ=12)
                                                                                                             cGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGgcgagcg                                          <  2:4030698/150‑1 (MQ=32)
                                                                                                               aaTCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGCAGGGCGagcgaa                                        >  1:5681036/1‑150 (MQ=17)
                                                                                                                 tCCGGCACGACCCACCAATGTAAAAAAGCGCCCCAACGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagc                                      <  2:126943/150‑1 (MQ=12)
                                                                                                                   cTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGAGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgcagcga                                    >  1:2493069/1‑150 (MQ=17)
                                                                                                                    tGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTAGAGTTGAGCGCATAGAAACAACTCAGCCGCACGCCGCGACGGCCCGAAGGGCGAGCGAAGCGAg                                   >  1:4089572/1‑150 (MQ=11)
                                                                                                                      cACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGGTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTc                                 >  2:5866175/1‑150 (MQ=17)
                                                                                                                      cACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACATGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTc                                 >  2:2143291/1‑150 (MQ=17)
                                                                                                                         gACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGACACCACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCagc                              >  2:6086528/1‑148 (MQ=12)
                                                                                                                           cccACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCt                            <  2:4747715/150‑1 (MQ=32)
                                                                                                                              cagatagTAAAAAAGCGCCTTTAGGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGGTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCa                         >  2:3177960/7‑150 (MQ=11)
                                                                                                                              accaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCa                         >  2:3620198/1‑150 (MQ=32)
                                                                                                                              accaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCa                         >  2:2565127/1‑150 (MQ=32)
                                                                                                                               ccaATGAAAAAACCCCCCCTAACGGCTCTTTTTTACTATCTGCTATAATAAAAGATTCTAACCTGCAGCTGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCAc                        <  1:2893605/150‑1 (MQ=11)
                                                                                                                                caATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACg                       >  2:1041371/1‑150 (MQ=32)
                                                                                                                                 aaTGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGa                      <  2:2320327/150‑1 (MQ=32)
                                                                                                                                     tAAAAAAGCGCCTTTAGGGCGCTTTTTTAAAATCTGAGAAACACAAAGATTCGAACCTGCAGCAGGTATGAGTTGAGCGCAGCGAAACAAACGAGCCCCGCGCCGCGACGGCCCGAAGGGCGAGCGACGCGAGTAATACAGCACGAcccc                  >  1:4989519/1‑149 (MQ=11)
                                                                                                                                     tAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGACGGGCGAGCGAAGCGAGTCATCCTGCACGACcca                  >  2:1991424/1‑150 (MQ=17)
                                                                                                                                      aaaaacGCGCCCTAACGGCGTTTTTTTAGTTTCGGCGAAACTCAATTATCCGAACCTGCAGCCGGTTTGTGTTGAGGGCCGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccac                 <  1:7064327/150‑1 (MQ=11)
                                                                                                                                      aaaaaaGCGCCTTTAGGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAAAGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccac                 >  1:4483615/1‑150 (MQ=255)
                                                                                                                                      aaaaaaGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccac                 >  2:5153525/1‑150 (MQ=32)
                                                                                                                                      aaaaaaGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccac                 >  2:4758651/1‑150 (MQ=32)
                                                                                                                                       aaaaaGCGCCTTTAGGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGAcccccc                >  2:4556442/1‑150 (MQ=255)
                                                                                                                                       aaaaaGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacc                >  1:7153448/1‑150 (MQ=32)
                                                                                                                                       aaaaaGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCGGCCGCCGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacc                >  2:6985101/1‑150 (MQ=12)
                                                                                                                                         aaaCCGCCCTAACGGCGTTTTTTTACTATCTGCGATACTAAATTTTTCGAACCTGCAGCAGTTTTGTGTTGAGCGCAGCCAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCaa              <  1:3559341/150‑1 (MQ=11)
                                                                                                                                           accgcCCTACAGGCGATTTTTTATTATCTGCTAAACTAAATTATTCTAACCTGCAGCTGTTTTGTGTTGCGCGCAGCCAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATg            <  1:2710348/148‑1 (MQ=11)
                                                                                                                                             cgcCCTACGGGCGCTTTTTTACTTTCTGCGATACTCAATGTTTGGACCCTGCCGCTGGTTTGTGTTGGGCGCGGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGtn          <  1:257374/150‑2 (MQ=11)
                                                                                                                                              gcCCTAAAGGCGCTTTTTTACTATCTGCTATACTCAAAGATTCGAACCTGCAGCAGTTTTGAGTTGAGCGCAGCTAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTaa         <  2:1549570/150‑1 (MQ=12)
                                                                                                                                                ccTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTaaaa       >  2:4185472/1‑150 (MQ=32)
                                                                                                                                                ccTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTaaaa       >  2:2681644/1‑150 (MQ=32)
                                                                                                                                                 cTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTaaaaa      >  2:6058282/1‑150 (MQ=32)
                                                                                                                                                  tAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTaaaaaa     >  1:3963873/1‑150 (MQ=32)
                                                                                                                                                    aaGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAgc   <  2:3208169/150‑1 (MQ=32)
                                                                                                                                                    aaGGCGCTTTTTTAATATATGCGATACTAAAAGATTAGAACCAGCAGCAGGTTTTAGTTGAGCGCAGCGAAACAAACGAGCCGCAAGCGGCGACGGCCCCAAGGGCGCGCGAAGCGAGTCATCCAGCACGACCCACCAAAGTAAAAAAgc   >  1:4865609/1‑150 (MQ=11)
                                                                                                                                                     |                                                                                                                                                     
CCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCG  >  NC_000913/2521199‑2521497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: