Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,334,704 G→C 6.0% intergenic (+23/‑205) ispB → / → sfsB octaprenyl diphosphate synthase/malPQ operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,334,7040GC 6.0% 1392.9 / 57.9 393intergenic (+23/‑205)ispB/sfsBoctaprenyl diphosphate synthase/malPQ operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (192/177);  new base C (16/8);  total (208/185)
Fisher's exact test for biased strand distribution p-value = 2.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

Only 100 of 401 total aligned reads displayed.

GACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTGAT  >  NC_000913/3334558‑3334853
                                                                                                                                                  |                                                                                                                                                     
gACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAgcgc                                                                                                                                                    >  2:3860869/1‑150 (MQ=255)
   gcgTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGcgcgcg                                                                                                                                                 <  2:565585/150‑1 (MQ=255)
   gcgTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGcgcgcg                                                                                                                                                 >  1:174276/1‑150 (MQ=255)
    cgTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGTACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCgg                                                                                                                                                >  1:1423768/1‑150 (MQ=255)
       cAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGAt                                                                                                                                             <  1:6472232/150‑1 (MQ=255)
       cAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTGGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGAt                                                                                                                                             <  1:4265971/150‑1 (MQ=255)
         gCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATgg                                                                                                                                           <  1:5211861/150‑1 (MQ=255)
           gTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCCCATCCCGCGCGCTGCGCGGGATgagg                                                                                                                                         <  2:3101506/150‑4 (MQ=255)
            tGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCCCCTCCCGCGCGCTGCGCGGGATgaggg                                                                                                                                        <  2:842517/150‑5 (MQ=255)
            tGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCCCATCCCGCGCGCTGCGCGGGATgaggg                                                                                                                                        <  2:128105/150‑5 (MQ=255)
              ccGAGGAAGAAGCAGACAAAGCCATCTCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCa                                                                                                                                      <  2:2836203/150‑1 (MQ=255)
              ccGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCa                                                                                                                                      <  2:1400188/150‑1 (MQ=255)
              ccGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCa                                                                                                                                      <  2:3208207/150‑1 (MQ=255)
              ccGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCa                                                                                                                                      <  2:2920895/150‑1 (MQ=255)
               cGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAg                                                                                                                                     >  2:1879227/1‑150 (MQ=255)
                gAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGATGGTTCCAGt                                                                                                                                    >  1:872968/1‑150 (MQ=255)
                gAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGt                                                                                                                                    >  1:2781469/1‑150 (MQ=255)
                gAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGt                                                                                                                                    >  1:1340263/1‑150 (MQ=255)
                 aGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTa                                                                                                                                   <  2:5584991/150‑1 (MQ=255)
                   gaagaaGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGATGGTTCCAGTAAg                                                                                                                                 >  2:3675778/1‑150 (MQ=255)
                   gaagaaGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAg                                                                                                                                 >  1:6657415/1‑150 (MQ=255)
                   gaagaaGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAg                                                                                                                                 <  1:2080289/150‑1 (MQ=255)
                      gaagCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTc                                                                                                                              >  1:2350658/1‑150 (MQ=255)
                      gaagCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTc                                                                                                                              >  1:3968767/1‑150 (MQ=255)
                        agCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCa                                                                                                                            <  2:10326/150‑1 (MQ=255)
                         gCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACCCCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGATGGTTCCCGTACGTTCCAt                                                                                                                           >  1:154795/1‑150 (MQ=255)
                         gCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGATGGTTCCAGTAAGTTCCAt                                                                                                                           >  1:3070079/1‑150 (MQ=255)
                         gCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGCTGGTTCCAGTAAGTTCCAt                                                                                                                           >  1:6515192/1‑150 (MQ=255)
                          cAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATa                                                                                                                          >  2:6041341/1‑150 (MQ=255)
                           aGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATaa                                                                                                                         <  2:642092/150‑1 (MQ=255)
                                 aGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAacac                                                                                                                   >  2:3481870/1‑150 (MQ=255)
                                 aGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAacac                                                                                                                   <  1:6289837/150‑1 (MQ=255)
                                    cATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTa                                                                                                                >  1:5908001/1‑150 (MQ=255)
                                    cATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTa                                                                                                                >  2:3791297/1‑150 (MQ=255)
                                        gCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTc                                                                                                                                        <  2:436481/122‑1 (MQ=255)
                                           gCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGct                                                                                                         <  1:4321891/150‑1 (MQ=255)
                                                aCAGGTGCTCCCGGACAACCCTTGGCGAGAAGAACACAACGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCtcta                                                                                                    >  2:3896558/1‑150 (MQ=255)
                                                    gTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAacac                                                                                                <  2:243322/150‑1 (MQ=255)
                                                     tGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTAAGCTCTATAACAcc                                                                                               >  1:1969555/1‑150 (MQ=255)
                                                         cccGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCATCGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGaa                                                                                           <  1:5845439/150‑1 (MQ=255)
                                                         cccGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGaa                                                                                           <  1:5616548/150‑1 (MQ=255)
                                                              acacCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATa                                                                                      <  2:5968491/150‑1 (MQ=255)
                                                               cacCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAg                                                                                     <  2:5731629/150‑1 (MQ=255)
                                                                  cccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGa                                                                                  >  1:5674408/1‑150 (MQ=255)
                                                                   ccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGAt                                                                                 <  1:5816506/150‑1 (MQ=255)
                                                                   ccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGAt                                                                                 >  1:990020/1‑150 (MQ=255)
                                                                   ccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGAt                                                                                 >  1:4423725/1‑150 (MQ=255)
                                                                    cTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATACCACCTGAAAAATAGGGAtg                                                                                <  2:6219056/150‑1 (MQ=255)
                                                                     ttGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGt                                                                                                                                >  1:3142871/1‑101 (MQ=255)
                                                                     ttGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGTGAtgt                                                                               >  2:2729108/1‑150 (MQ=255)
                                                                      tGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGAtgtg                                                                              <  1:1692746/150‑1 (MQ=255)
                                                                         cGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCAt                                                                           <  1:2924072/150‑1 (MQ=255)
                                                                              aGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTta                                                                      <  1:725433/150‑1 (MQ=255)
                                                                               gCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtat                                                                     <  2:6969329/150‑1 (MQ=255)
                                                                                    cATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCACTATAAAACCTGAAAAATAGGGATGTGCATCGTTATATAtt                                                                >  1:3536655/1‑150 (MQ=255)
                                                                                     aTCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTc                                                               <  1:6818679/150‑1 (MQ=255)
                                                                                         gCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGATGGTTCCAGTAAGTTACATAAAAAAACTTATTAAGCTCTATAACACCAGAAAAATAGGGATGTGCATCTTTATATATTCTGaa                                                           >  1:508553/1‑150 (MQ=255)
                                                                                            tCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAatat                                                        >  2:6251635/1‑150 (MQ=255)
                                                                                            tCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCAATAACACCAGAAAAAAAGGGATGTGGATCTTTATATATTATGAatat                                                        >  1:2573337/1‑150 (MQ=255)
                                                                                            tCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGGACCAGAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGCTGTGCATCGTGATATAGTCTGAagat                                                        >  2:5483351/1‑150 (MQ=255)
                                                                                               cgcACATCGCTTTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTca                                                     <  1:4685875/150‑1 (MQ=255)
                                                                                                gcACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTcac                                                    <  1:7096715/150‑1 (MQ=255)
                                                                                                gcACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTcac                                                    >  2:2205820/1‑150 (MQ=255)
                                                                                                    aTCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCCCGCAGCGCGCGGGTTGGTTCCAGTAAATTCCAAAAAAAAAAATATTAAGCTCTATAAAACCAGAAAAATAGGGATGTTCATCTTTATATATTATGAATATTAacacac                                                >  1:2834957/1‑148 (MQ=255)
                                                                                                       gCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCCGGCTGGGTCCAGTAAGGTACAAAAAAAAACGTATTAAGCTCTATAAAACCAGAAAAAAAGGGTTGTGCATCGGTATATATTATGTATATTAacacacgtt                                             >  1:2681756/1‑145 (MQ=255)
                                                                                                           ttCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAg                                         >  2:2994855/1‑150 (MQ=255)
                                                                                                              aaCGCGATCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGGTGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCAGAAAAATAGGGGTGTGCATCTTTATATATTATGAATATTCACACTCTTTACAGGaa                                      >  1:3796378/1‑150 (MQ=255)
                                                                                                              aaCGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGaa                                      <  2:2372592/150‑1 (MQ=255)
                                                                                                                cgcgATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACt                                    >  2:2477635/1‑150 (MQ=255)
                                                                                                                  cgATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGAGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCAATAACACCTGAAAAGAAGGGATGTGCATCTTTATATCTTCTGAATATTCACACTCATTACAGCAACttt                                  >  2:6579224/1‑150 (MQ=255)
                                                                                                                   gATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACtttt                                 >  2:5123809/1‑150 (MQ=255)
                                                                                                                   gATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTAGTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTGTATCTAGTCTGAATATTCACACTCTGTACAGGAACtgtg                                 >  2:6451043/1‑147 (MQ=255)
                                                                                                                    aTCGATAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACttttt                                >  2:930484/1‑150 (MQ=255)
                                                                                                                     tCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTa                               <  1:2168235/150‑1 (MQ=255)
                                                                                                                     tCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTa                               <  2:4247371/150‑1 (MQ=255)
                                                                                                                       gTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCAGCTTTATATATTCTGAATATTCACACTCGTTACAGGAACTGTTTaga                             >  2:6783946/1‑150 (MQ=255)
                                                                                                                        ttAATCCCCTCCCCTCCGCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTagag                            <  2:4801186/150‑1 (MQ=255)
                                                                                                                        ttAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTagag                            <  2:4689328/150‑1 (MQ=255)
                                                                                                                              ccctcccctcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAg                      >  2:3380505/1‑150 (MQ=255)
                                                                                                                               cctcccctcATCCCGCGCAGCGCGGGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTAAGCTCTTTAACACCTGAAAATTAGGTATGTGGATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTTGTGCAATatg                     >  2:4281044/1‑148 (MQ=255)
                                                                                                                                ctcccctcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGc                    >  2:2589120/1‑150 (MQ=255)
                                                                                                                                    cctcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATc                <  2:3692414/150‑1 (MQ=255)
                                                                                                                                     ctcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGTCCATCa               >  2:4918222/1‑150 (MQ=255)
                                                                                                                                       cATCCCGCGCGCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAgg             >  2:5265015/1‑150 (MQ=255)
                                                                                                                                        aTCCCGCGCGCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa            >  2:4573241/1‑150 (MQ=255)
                                                                                                                                        aTCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa            >  1:3424389/1‑150 (MQ=255)
                                                                                                                                        aTCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa            <  2:1144374/150‑1 (MQ=255)
                                                                                                                                        aTCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCAAACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa            <  2:6700946/150‑1 (MQ=255)
                                                                                                                                        aTCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATCCACACTCTTTACAGGAACTTTTTAGAGTAATAGGCCATCAGGa            <  2:1654597/150‑1 (MQ=255)
                                                                                                                                         tCCCGCGCGCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAg           >  1:4103373/1‑150 (MQ=255)
                                                                                                                                         tCCCGCGCGCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAg           >  2:1967861/1‑150 (MQ=255)
                                                                                                                                         tCCCGCGCGCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAg           >  1:1271606/1‑150 (MQ=255)
                                                                                                                                         tCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCACTAGGCCCTCAGGAg           >  2:5942920/1‑150 (MQ=255)
                                                                                                                                         tCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAg           >  1:3778768/1‑150 (MQ=255)
                                                                                                                                         tCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCAGCAGGAg           >  1:5483669/1‑150 (MQ=255)
                                                                                                                                         tCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCAGAAAAATAGGGATGTGCATATTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAg           >  1:1803748/1‑150 (MQ=255)
                                                                                                                                          cccGCGCGCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGt          >  2:4210352/1‑150 (MQ=255)
                                                                                                                                               gcAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGt     <  2:4423725/150‑1 (MQ=255)
                                                                                                                                                cAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTg    <  1:6041341/150‑1 (MQ=255)
                                                                                                                                                 aGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTGa   <  1:1031261/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTGAT  >  NC_000913/3334558‑3334853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: