Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 380,004 A→C 5.9% intergenic (‑123/+65) frmR ← / ← yaiO regulator protein that represses frmRAB operon/outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913380,0040AC 5.9% 771.3 / 2.3 251intergenic (‑123/+65)frmR/yaiOregulator protein that represses frmRAB operon/outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (204/24);  new base C (5/18);  total (209/42)
Fisher's exact test for biased strand distribution p-value = 5.42e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 266 total aligned reads displayed.

TTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTC  >  NC_000913/379855‑380153
                                                                                                                                                     |                                                                                                                                                       
tttCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACaa                                                                                                                                                         <  1:6892402/150‑1 (MQ=255)
 ttcttcTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACaaa                                                                                                                                                        <  2:4211204/150‑1 (MQ=255)
 ttcttcTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAAGGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACaaa                                                                                                                                                        <  2:2547513/150‑1 (MQ=255)
   cttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAaga                                                                                                                                                      <  1:1299009/150‑1 (MQ=255)
    ttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGGGAATGTGGTCGGAAAACAAcgag                                                                                                                                                     >  1:467010/1‑150 (MQ=255)
       tcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGaa                                                                                                                                                  >  1:1716804/1‑150 (MQ=255)
       tcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGaa                                                                                                                                                  >  1:4216880/1‑150 (MQ=255)
       tcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGaa                                                                                                                                                  >  1:2888523/1‑150 (MQ=255)
         ttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAg                                                                                                                                               >  1:1916916/1‑150 (MQ=255)
         ttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAg                                                                                                                                               >  1:3358339/1‑150 (MQ=255)
            cGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAAAATACTATAGGCGGGAATTCTATATCTCAATGCATACCCCCCTATAGTATAAAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggg                                                                                                                                            >  2:6082092/1‑150 (MQ=255)
                  acgtggCTTTTCTCACCTCATAATCTGCTGGCAATTTACTCTCGGGGGGTATTCTATATTTCAATGCATACCCCCCTATAGTATTTAACCCTGGAGAATAAATTTTCGCCGTTGATTGTGGTGGTAAAACAACGCGGAAAgggggggggg                                                                                                                                      <  2:652469/146‑1 (MQ=255)
                     gggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGACAACACAGAGGACAGGGGGGGGGGCTa                                                                                                                                   >  1:1035188/1‑150 (MQ=255)
                     gggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTa                                                                                                                                   >  1:1035547/1‑150 (MQ=255)
                     gggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTa                                                                                                                                   >  1:6445609/1‑150 (MQ=255)
                      ggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTaa                                                                                                                                  >  1:3289487/1‑150 (MQ=255)
                      ggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCAGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTaa                                                                                                                                  >  2:1462887/1‑150 (MQ=255)
                        cATTTCTAACCTCTTAATTTGCATTCAATATACTATTGGGTGTTATTCTATATGTAAATTCATACCCCCCTTTTTTTTTTAACACTGGAGAATAAAATTTATCCGGTGAATGTGGGCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATc                                                                                                                                <  2:2746697/150‑1 (MQ=255)
                        cATGTCGCACCTCATCATCTCCATGCAATATACGATCGGGGGGTATTCTCTATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTAGCCGGTGAATGTGGTCGGAAAACACAGAGGAAAGGGGGGGGGGCTAATc                                                                                                                                <  2:7043126/150‑1 (MQ=255)
                         aTTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCg                                                                                                                               >  2:6624169/1‑150 (MQ=255)
                           ttCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGc                                                                                                                             >  1:2766920/1‑150 (MQ=255)
                            tCGCACCTCTTCATCTGCATGCAATATACTATAGGGGGGTATTCAATATGTCAATGCATACCCCCATATAGTATATAACACTGGAGAATAAAATTTCTCCGGTGAAGGTGGTGGGAAAACACCGAGGAAAGGGGGGGGGGCTAATCGGCa                                                                                                                            <  2:4046322/150‑1 (MQ=255)
                            tCGCACCTCATCATCTGCATGCTATATACTATCGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTTTATAACACTGGAGAATAAAATTTATCCGGTGAATGGGGTCGGAAAACAACGAGGACAGGGGGGGGGGCTAATCGGCa                                                                                                                            <  2:3727250/150‑1 (MQ=255)
                               cACCTGATCATCTGCCTGCAATTTCAGATTGGGGGGTAGTCTATATGTGAAGGCATACCCCACCAGATAATTTACCACGTTAGTATACCCTTTTTCCGGTGCATGTGGTCGGACAACCAAGACGAAAGGGGGGGGGGCTAATCGGCAggg                                                                                                                         <  2:6761980/150‑1 (MQ=255)
                                aCCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGa                                                                                                                        >  2:1557525/1‑150 (MQ=255)
                                aCCTCATCATCTGCAGGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGACAGGGGGGGGGGCGAATCGGCCgggc                                                                                                                        >  2:5133238/1‑149 (MQ=255)
                                  cTCATCTTCTGCATGCAATATACTATAGGGGGGTTTTCTATATGTAATTGCATACCCCCCTATAGTATTTAACACTGGAGAATAAAATTTATCCGGTGAAGGTGGTCGGAAAACACCGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAg                                                                                                                      <  2:872093/150‑1 (MQ=255)
                                         tcTGCATGCAATAGACTATAGTGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACACAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGcccc                                                                                                              <  2:5285649/150‑1 (MQ=255)
                                          cggcatgcaCTCTACTCCCGGGGGGTATCCTCCCTGCCACGGCATACCCCCCGATCGTATCTAACCCTGGCGAATAAACTTCCCCCGGGGAAGGGGGCCGGACACCCCCGCGGACCGGGGGGGGGGCTAATCGGCAGGGAAGGCCGcccc                                                                                                              <  2:3384666/148‑1 (MQ=255)
                                          cTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAATGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGcccc                                                                                                              >  1:5194610/1‑150 (MQ=255)
                                             catgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGGAGAAAATTTATCCGGTGACTGTGGTCGGAAAACAAAGAGGCAAGGGGGGGGGGCTAAGCGGCGGGGAAGGCCGCCCCGGa                                                                                                           >  2:227928/1‑150 (MQ=255)
                                             catgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGACAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGa                                                                                                           >  2:4530076/1‑150 (MQ=255)
                                              atgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATa                                                                                                         >  1:6031081/1‑150 (MQ=255)
                                                gcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAg                                                                                                        >  1:6647988/1‑150 (MQ=255)
                                                 caATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgc                                                                                                       >  1:3476112/1‑150 (MQ=255)
                                                 caATATACAATAGGGGGGTATTCTAAATCACAATGCATACCCCCCTATAGTATATAACACTCGACAAAAAAAATTACCCGGTGAATGTGGACGGAAACCAAACCCCAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgc                                                                                                       <  2:360552/150‑1 (MQ=255)
                                                  actataCTAGCGGGGGGTATACTATATGTCAATGCATCCCCCCCTATAGGATACAACCCGGGAGAATAAAATTTAGCCGGTGCATGTGGACGGAAAACACAGCGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcg                                                                                                      <  2:6367383/148‑1 (MQ=255)
                                                  aaTATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcg                                                                                                      >  2:4672970/1‑150 (MQ=255)
                                                  aaTATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcg                                                                                                      >  1:5765366/1‑150 (MQ=255)
                                                  aaTATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATAGAACGCTGGAGAGGAAAATTTATCCGGTGAATGTGGTCGGACGACGATGAGGACCGGGGGGGGGGCTAAGCGGCAGGGACGGCCGCCCCGGCTGgcg                                                                                                      >  1:1575817/1‑150 (MQ=255)
                                                    tataCTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAGAACAATGAGGAAAGGGGGGGGGGGGTAATCGGCAGGGAAGGCCGCCCCGGTTAgcgg                                                                                                     >  2:1391389/1‑150 (MQ=255)
                                                    tataCTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcggg                                                                                                    >  2:886024/1‑150 (MQ=255)
                                                    tataCTATAGGGGGGTATTCTAAATGTCACTGCATACCCCCCTATAGTATATAACCCTGGAGAATAAAATTTATCCGGTGAACGTGGGCGGAAACCAAAGCGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcggg                                                                                                    <  2:2882310/150‑1 (MQ=255)
                                                    aataCTATAGGGGTGTATAATCTCTGTCAAGGCAAACCCCCCTATAGCAAATAACCCTGGACAAAAAAATCTCCCCGCTGCCGGGGGCCGGCCCCCAACGCGCAACGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcggg                                                                                                    <  2:3213143/149‑1 (MQ=255)
                                                         tataGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGACGGCCGCCCCGGATAGCGGGCGGCa                                                                                              >  2:5765360/1‑150 (MQ=255)
                                                         tataGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGggcggcc                                                                                              >  1:2174217/1‑149 (MQ=255)
                                                         tataGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATAGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCa                                                                                              >  2:1971125/1‑150 (MQ=255)
                                                              gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACACAGAGGAAAGGGGGGGGGGCGCATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCCGAAgg                                                                                         >  2:6214566/1‑150 (MQ=255)
                                                              gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAgg                                                                                         >  2:3074978/1‑150 (MQ=255)
                                                               ggggCATTCTACCTGGCCAGGCATCCCCCCCTATAGTCTACCCCCCGGGAGCAAAAAATATCTCCGGTGAATGGTGCCGGCAACCAACGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGa                                                                                        <  2:1019726/150‑1 (MQ=255)
                                                                     ttCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCGAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAACAGa                                                                                  >  1:5547407/1‑150 (MQ=255)
                                                                      tCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGaa                                                                                 >  2:12967/1‑150 (MQ=255)
                                                                        tataTGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAtt                                                                               >  1:4858265/1‑150 (MQ=255)
                                                                        tataTGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCCGGTAGGCGGCGGCCggaaggaaccggatt                                                                                >  2:5594984/1‑143 (MQ=255)
                                                                           atGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTcc                                                                            >  2:5026778/1‑150 (MQ=255)
                                                                           atGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTcc                                                                            >  1:5070625/1‑150 (MQ=255)
                                                                           atGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATAGGCAGGGAAGGCCCCCCCGGTTATCGGGCGGCAGAAGGAAACAGTATTTcc                                                                            >  1:1442024/1‑150 (MQ=255)
                                                                                aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGACTCAGAATTTCCAGGTc                                                                       >  1:5727049/1‑150 (MQ=255)
                                                                                aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTc                                                                       >  2:7183264/1‑150 (MQ=255)
                                                                                aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTc                                                                       >  1:1119892/1‑150 (MQ=255)
                                                                                aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTc                                                                       >  1:654155/1‑150 (MQ=255)
                                                                                   gCATACCCCCCTAGAGTATATAACACTGGAGAATAAAATGTATCCGGTGAATGTGGTCGGAAAACAACGCGGACAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGa                                                                    <  2:4701645/150‑1 (MQ=255)
                                                                                     aTACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACg                                                                  >  2:2075495/1‑150 (MQ=255)
                                                                                           cccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc                                                            >  1:4495088/1‑150 (MQ=255)
                                                                                           cccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc                                                            >  2:1503638/1‑150 (MQ=255)
                                                                                           cccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATGTCCAGGTCAGACGGGCTGc                                                            >  2:3869945/1‑150 (MQ=255)
                                                                                             cTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCaa                                                          >  1:3637823/1‑150 (MQ=255)
                                                                                             cTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAg                                                         >  2:4426942/1‑150 (MQ=255)
                                                                                               ataGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGt                                                        >  2:1140266/1‑150 (MQ=255)
                                                                                                 aGTATATAACCCCGCACAATAAAATTCCTCCGGCGAAGGGGGCCGGAAAACAAACAGCAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTg                                                      <  2:6168674/150‑1 (MQ=255)
                                                                                                  gTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc                                                     >  2:4382882/1‑150 (MQ=255)
                                                                                                   tatatcACCCTGGAGAATAACAATTACCCGGTGACCGTGGCCGCAAACCACCCCCGACCGGGGGGGGGGCCAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa                                                    <  2:4085744/150‑1 (MQ=255)
                                                                                                     tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAc                                                 >  1:2392036/1‑150 (MQ=255)
                                                                                                      ataACACTCGAGAATAAAAATAAACCCCTCAATCCCGACCGAAAACAACCCCCAAAGGGGGGGGGGATAATCGGCAGGGAAGGCCACCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAc                                                 <  2:5786052/150‑1 (MQ=255)
                                                                                                        aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCg                                               >  2:3228440/1‑150 (MQ=255)
                                                                                                        aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCg                                               >  2:5074024/1‑150 (MQ=255)
                                                                                                               gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAat                                        >  1:6265454/1‑150 (MQ=255)
                                                                                                                    tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGACTCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCg                                   >  2:5430201/1‑150 (MQ=255)
                                                                                                                    tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCg                                   >  2:1718706/1‑150 (MQ=255)
                                                                                                                      aaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGACTCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGt                                 >  1:2514318/1‑150 (MQ=255)
                                                                                                                       aaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAACGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGtt                                >  2:1112897/1‑150 (MQ=255)
                                                                                                                        aTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTg                               >  2:782464/1‑150 (MQ=255)
                                                                                                                             tCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgtg                          >  1:2945950/1‑150 (MQ=255)
                                                                                                                             tCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAACCAGAATTTCCCGGTCAGACGGGCTGCAAGGTGCAGACCGTTAAAATCATCgggtggggtg                          >  2:3108321/1‑150 (MQ=255)
                                                                                                                               cGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTc                        >  1:2996437/1‑150 (MQ=255)
                                                                                                                               cGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATAGGTTGGGGTGTc                        >  1:2495312/1‑150 (MQ=255)
                                                                                                                                ggTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCg                       >  2:890399/1‑150 (MQ=255)
                                                                                                                                ggTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCg                       >  1:3193130/1‑150 (MQ=255)
                                                                                                                                  tGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTa                     >  1:2194418/1‑150 (MQ=255)
                                                                                                                                          gTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACtt             >  1:5273521/1‑150 (MQ=255)
                                                                                                                                          gTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACtt             >  1:7218000/1‑150 (MQ=255)
                                                                                                                                           tCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACttt            >  2:542811/1‑150 (MQ=255)
                                                                                                                                           tCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACttt            >  1:3061673/1‑150 (MQ=255)
                                                                                                                                              gAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTAcc         >  1:353143/1‑150 (MQ=255)
                                                                                                                                              gAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAATTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTAcc         >  1:4016580/1‑150 (MQ=255)
                                                                                                                                               aaaaCAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCt        >  2:1002611/1‑150 (MQ=255)
                                                                                                                                                 aaCAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGc      >  1:2634102/1‑150 (MQ=255)
                                                                                                                                                   cAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCg    >  1:497001/1‑150 (MQ=255)
                                                                                                                                                    aaaGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGt   >  1:4305049/1‑150 (MQ=255)
                                                                                                                                                     aaGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTc  >  2:3013423/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
TTTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTC  >  NC_000913/379855‑380153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: