Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,458,110 C→A 100% T578T (ACC→ACA gspD → general secretory pathway component, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,458,1100CA100.0% 1743.1 / NA 450T578T (ACC→ACAgspDgeneral secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (216/234);  total (216/234)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 458 total aligned reads displayed.

TACTATTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACCGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGT  >  NC_000913/3457963‑3458256
                                                                                                                                                   |                                                                                                                                                  
tACTATTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGa                                                                                                                                                  <  1:2788020/150‑1 (MQ=255)
tACTATTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGa                                                                                                                                                  <  1:2413830/150‑1 (MQ=255)
  cTATTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAgc                                                                                                                                                <  2:4581688/150‑1 (MQ=255)
   tATTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAgcg                                                                                                                                               <  1:1668034/150‑1 (MQ=255)
     ttCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAgcgcg                                                                                                                                             >  1:3016473/1‑150 (MQ=255)
     ttCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAgcgcg                                                                                                                                             <  1:2527713/150‑1 (MQ=255)
     ttCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAgcgcg                                                                                                                                             <  2:2477095/150‑1 (MQ=255)
      tCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAgcgcgc                                                                                                                                            <  1:5124561/150‑1 (MQ=255)
      tCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAgcgcgc                                                                                                                                            <  2:6088405/150‑1 (MQ=255)
       cAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCt                                                                                                                                           >  2:7157622/1‑150 (MQ=255)
       cAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCt                                                                                                                                           <  2:6427950/150‑1 (MQ=255)
        aaaaCGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTa                                                                                                                                          >  1:6244183/1‑150 (MQ=255)
          aaCGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTaaa                                                                                                                                        <  2:6794431/150‑1 (MQ=255)
           aCGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAAc                                                                                                                                       <  2:2890796/150‑1 (MQ=255)
           aCGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAAc                                                                                                                                       <  1:4887769/150‑1 (MQ=255)
           aCGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAAc                                                                                                                                       <  1:3437015/150‑1 (MQ=255)
               cGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCaa                                                                                                                                   <  1:2661402/150‑1 (MQ=255)
               cGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCaa                                                                                                                                   >  1:6344501/1‑150 (MQ=255)
               cGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCaa                                                                                                                                   >  1:3684121/1‑150 (MQ=255)
                gTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAAc                                                                                                                                  <  2:6262868/150‑1 (MQ=255)
                 tGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAAcc                                                                                                                                 <  2:4109279/150‑1 (MQ=255)
                  gCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTCTACCTCCACAGAGCGCGCTAAACGCAACCt                                                                                                                                >  2:3205880/1‑150 (MQ=255)
                   cTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTg                                                                                                                               >  1:1451885/1‑150 (MQ=255)
                    tGGTCAACACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATAGTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGa                                                                                                                              <  2:6631058/150‑1 (MQ=255)
                    tGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGa                                                                                                                              >  1:4658977/1‑150 (MQ=255)
                      gTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATg                                                                                                                            >  1:5776719/1‑150 (MQ=255)
                      gTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATg                                                                                                                            >  1:6761332/1‑150 (MQ=255)
                      gTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATg                                                                                                                            >  1:920081/1‑150 (MQ=255)
                         aaaaCCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTa                                                                                                                         <  1:1133005/150‑1 (MQ=255)
                          aaaCCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTAt                                                                                                                        >  1:2152155/1‑150 (MQ=255)
                              cGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTAt                                                                                                                    >  2:5207814/1‑150 (MQ=255)
                                gTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTAtcc                                                                                                                  <  2:2819904/150‑1 (MQ=255)
                                gTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTAtcc                                                                                                                  <  1:6837840/150‑1 (MQ=255)
                                  gAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTAtccgt                                                                                                                <  2:2140312/150‑1 (MQ=255)
                                  gAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTAtccgt                                                                                                                <  2:1331983/150‑1 (MQ=255)
                                           gtCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGAtt                                                                                                       <  2:5019360/150‑1 (MQ=255)
                                            tCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTa                                                                                                      >  2:2176138/1‑150 (MQ=255)
                                               tGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATcc                                                                                                   >  1:5352369/1‑150 (MQ=255)
                                               tGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATcc                                                                                                   >  2:4252068/1‑150 (MQ=255)
                                                gggCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCg                                                                                                  >  1:5997851/1‑150 (MQ=255)
                                                   cGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGa                                                                                               <  2:5895819/150‑1 (MQ=255)
                                                      aTTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTCTCCGTCCGACGATTATCCGTGACga                                                                                            >  1:4596324/1‑150 (MQ=255)
                                                        tGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACgatg                                                                                          <  1:142251/150‑1 (MQ=255)
                                                             gatgatTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGtt                                                                                     >  2:2006282/1‑150 (MQ=255)
                                                             gatgatTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGtt                                                                                     <  1:5563506/150‑1 (MQ=255)
                                                              atgatTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGttt                                                                                    <  2:2990070/150‑1 (MQ=255)
                                                                gatTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTAt                                                                                  <  2:5173817/150‑1 (MQ=255)
                                                                gatTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTAt                                                                                  >  1:3223030/1‑150 (MQ=255)
                                                                  tttttCTTAAGATGATGTGTCATTGGTTCCTCTGCTTGGAGATTTTCCTTTAGTGGGTCAACTCTTCCGCTATACCTCCACAGCGAGCGCTATACGCATCCTGATGGTTTTTCTCAGTCCGACGATTATCGGTTACGATTATGTTTATCg                                                                                <  2:766556/150‑1 (MQ=255)
                                                                  tttttCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCg                                                                                >  1:6962416/1‑150 (MQ=255)
                                                                  tttttCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCg                                                                                <  2:2284067/150‑1 (MQ=255)
                                                                    tttCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCt                                                                              <  1:4011230/150‑1 (MQ=255)
                                                                    tttCTAAAGAGCAAGTGACAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCt                                                                              <  2:4229544/150‑1 (MQ=255)
                                                                     ttCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTc                                                                             <  2:822828/150‑1 (MQ=255)
                                                                        tAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACt                                                                          <  1:7137257/150‑1 (MQ=255)
                                                                              gCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCaaa                                                                    >  1:1539861/1‑150 (MQ=255)
                                                                                 aGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAaga                                                                 <  1:7068114/150‑1 (MQ=255)
                                                                                   tgtCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAgaga                                                               <  1:3927181/150‑1 (MQ=255)
                                                                                   tgtCAAAGGTTCCTCTGCTTGGCGATATTCCTTGAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAgaga                                                               <  1:5695197/150‑1 (MQ=255)
                                                                                      cAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAAt                                                            <  2:3893343/150‑1 (MQ=255)
                                                                                       aaaGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATa                                                           >  2:1811933/1‑150 (MQ=255)
                                                                                        aaGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATac                                                          >  1:4378661/1‑150 (MQ=255)
                                                                                          ggTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGATCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATacac                                                        <  2:3764167/150‑1 (MQ=255)
                                                                                          ggTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATacac                                                        <  1:378386/150‑1 (MQ=255)
                                                                                            ttCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACAccc                                                      <  2:5720431/150‑1 (MQ=255)
                                                                                             tCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCg                                                     >  1:6152107/1‑150 (MQ=255)
                                                                                               ctctGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGtt                                                   >  1:2000474/1‑150 (MQ=255)
                                                                                                    cTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGt                                              <  2:3372132/150‑1 (MQ=255)
                                                                                                     ttGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTc                                             >  2:265468/1‑150 (MQ=255)
                                                                                                       ggCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAg                                           >  2:1249906/1‑150 (MQ=255)
                                                                                                         cGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGa                                         <  2:846735/150‑1 (MQ=255)
                                                                                                         cGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGa                                         <  2:3539113/150‑1 (MQ=255)
                                                                                                         cGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGa                                         <  1:3603472/150‑1 (MQ=255)
                                                                                                          gATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAg                                        >  2:3415066/1‑150 (MQ=255)
                                                                                                           atatTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGTTGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGc                                       <  1:4291767/150‑1 (MQ=255)
                                                                                                                  tttAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAacaaca                                >  1:1578313/1‑150 (MQ=255)
                                                                                                                   ttAGTGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAg                               >  2:2368692/1‑150 (MQ=255)
                                                                                                                       tGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGa                           <  1:5207814/150‑1 (MQ=255)
                                                                                                                       tGGGGCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGa                           >  2:5595812/1‑150 (MQ=255)
                                                                                                                           gCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGa                       >  2:3631331/1‑150 (MQ=255)
                                                                                                                           gCAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGa                       >  1:561475/1‑150 (MQ=255)
                                                                                                                            cAACTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGAc                      <  1:4976626/150‑1 (MQ=255)
                                                                                                                              aCTCTTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACgg                    <  1:5950289/150‑1 (MQ=255)
                                                                                                                                tctTCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGa                  >  1:73895/1‑150 (MQ=255)
                                                                                                                                  ttCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGaaa                <  2:6504096/150‑1 (MQ=255)
                                                                                                                                   tCCGCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAAt               >  1:3144407/1‑150 (MQ=255)
                                                                                                                                      gCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCaa            <  1:1299189/150‑1 (MQ=255)
                                                                                                                                      gCTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCaa            >  1:4474555/1‑150 (MQ=255)
                                                                                                                                       cTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCaaa           <  2:5982264/150‑1 (MQ=255)
                                                                                                                                       cTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCaaa           >  2:2741733/1‑150 (MQ=255)
                                                                                                                                       cTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCaaa           <  1:4932535/150‑1 (MQ=255)
                                                                                                                                       cTATACCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCaaa           >  1:288591/1‑150 (MQ=255)
                                                                                                                                          taCCTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAgc        <  1:6425989/150‑1 (MQ=255)
                                                                                                                                             cTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCt     <  2:1103330/150‑1 (MQ=255)
                                                                                                                                             cTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCt     >  1:1901900/1‑150 (MQ=255)
                                                                                                                                             cTCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCt     >  2:916994/1‑150 (MQ=255)
                                                                                                                                              tCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTg    >  1:858707/1‑150 (MQ=255)
                                                                                                                                              tCCACAGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTg    >  1:4022767/1‑150 (MQ=255)
                                                                                                                                                cacaGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGt  <  2:4819324/150‑1 (MQ=255)
                                                                                                                                                cacaGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGt  >  1:655547/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TACTATTCAAAACGCCGTGCTGGTCAAAACCGGTGAAACGGTGGTCCTGGGCGGATTGCTGGATGATTTTTCTAAAGAGCAAGTGTCAAAGGTTCCTCTGCTTGGCGATATTCCTTTAGTGGGGCAACTCTTCCGCTATACCTCCACCGAGCGCGCTAAACGCAACCTGATGGTATTTATCCGTCCGACGATTATCCGTGACGATGATGTTTATCGCTCACTGTCAAAAGAGAAATACACCCGTTACCGTCAGGAGCAACAACAGCGGATCGACGGGAAATCAAAAGCGCTGGT  >  NC_000913/3457963‑3458256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: