Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,470,109 A→G 5.5% intergenic (‑256/+36) chiA ← / ← tufA periplasmic endochitinase/translation elongation factor EF‑Tu 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,470,1090AG 5.5% 1389.3 / 46.3 417intergenic (‑256/+36)chiA/tufAperiplasmic endochitinase/translation elongation factor EF‑Tu 1
Reads supporting (aligned to +/- strand):  ref base A (202/190);  new base G (8/15);  total (211/206)
Fisher's exact test for biased strand distribution p-value = 1.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.87e-01

Only 100 of 426 total aligned reads displayed.

AGAAAAAAGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTAACAACCATTTT  >  NC_000913/3469960‑3470258
                                                                                                                                                     |                                                                                                                                                      
aGAAAAAAGCCTGACACATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGaa                                                                                                                                                        <  1:6316270/150‑1 (MQ=255)
    aaaaGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAgcgc                                                                                                                                                    <  2:841069/150‑1 (MQ=255)
    aaaaGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAgcgc                                                                                                                                                    <  1:5575902/150‑1 (MQ=255)
        gCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCtt                                                                                                                                                >  1:2636685/1‑150 (MQ=255)
         ccTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCttt                                                                                                                                               >  1:3098290/1‑150 (MQ=255)
          cTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCtttt                                                                                                                                              <  2:6442482/150‑1 (MQ=255)
          cTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCtttt                                                                                                                                              <  2:1002900/150‑1 (MQ=255)
          cTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTGAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCTTCGGCGCCCtttt                                                                                                                                              <  2:1152645/150‑1 (MQ=255)
           tGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttc                                                                                                                                             >  1:4422670/1‑150 (MQ=255)
             aCAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCTGCGGCGCCCtttttag                                                                                                                                           >  1:6237113/1‑147 (MQ=255)
              cAAATGACGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCGAAAAGGGCGCTTCGGCGCCCtttttgca                                                                                                                                          <  1:2814710/150‑5 (MQ=255)
              cAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttcaat                                                                                                                                          <  2:103487/150‑1 (MQ=255)
              cAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttcaat                                                                                                                                          <  2:5557508/150‑1 (MQ=255)
               aaaTGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttcaatt                                                                                                                                         >  2:4455911/1‑150 (MQ=255)
               aaaTGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttcaatt                                                                                                                                         <  1:6414564/150‑1 (MQ=255)
                aaTGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTttcaattc                                                                                                                                        >  2:7144921/1‑150 (MQ=255)
                       cATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTa                                                                                                                                 >  1:2447541/1‑150 (MQ=255)
                       cATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTa                                                                                                                                 >  1:2297715/1‑150 (MQ=255)
                          tttAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAATTGGGCGCCGAAGCGCCCTTTTCAATTCAATACTAAtt                                                                                                                              >  1:3593418/1‑150 (MQ=255)
                           ttAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAATCTAATTa                                                                                                                             >  2:5063827/1‑150 (MQ=255)
                               aaaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTATATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACgt                                                                                                                        <  1:6409175/150‑1 (MQ=255)
                               aaaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACgt                                                                                                                        >  2:3737081/1‑150 (MQ=255)
                               aaaCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACgt                                                                                                                        >  2:1217068/1‑150 (MQ=255)
                                 aCAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACgtgt                                                                                                                      <  1:1286020/150‑1 (MQ=255)
                                   aGAAACATTCATATTTAGAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTaa                                                                                                                    >  2:1348701/1‑150 (MQ=255)
                                   aGAAACATTCATATTTAAAATGTTAAATAGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTaa                                                                                                                    >  2:6949710/1‑150 (MQ=255)
                                     aaaCATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAAtt                                                                                                                  >  2:5288695/1‑150 (MQ=255)
                                        cATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGc                                                                                                               >  2:6869057/1‑150 (MQ=255)
                                              tatTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTACACGTTAATTAgttttgaa                                                                                                         <  1:1224720/150‑8 (MQ=255)
                                                tttAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACt                                                                                                       >  1:3911143/1‑150 (MQ=255)
                                                  tAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACttt                                                                                                     >  2:6804161/1‑150 (MQ=255)
                                                   aaaaTGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTa                                                                                                    <  2:420618/150‑1 (MQ=255)
                                                    aaaTGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAg                                                                                                   <  1:5297326/150‑1 (MQ=255)
                                                     aaTGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGc                                                                                                  <  2:1734183/150‑1 (MQ=255)
                                                     aaTGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATCCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGc                                                                                                  <  1:4936813/150‑1 (MQ=255)
                                                       tGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGcaa                                                                                                <  2:2672317/150‑1 (MQ=255)
                                                        gTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGcaac                                                                                               <  1:3359352/150‑1 (MQ=255)
                                                         ttAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGcaaca                                                                                              >  1:4825153/1‑150 (MQ=255)
                                                         ttAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGcaaca                                                                                              >  2:3982432/1‑150 (MQ=255)
                                                         ttAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCAAGAACTTAAGcaaca                                                                                              >  2:5875821/1‑150 (MQ=255)
                                                                gaattgaTATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCcg                                                                                       >  1:5738218/1‑150 (MQ=255)
                                                                 aattgaTATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCcgc                                                                                      >  1:538404/1‑150 (MQ=255)
                                                                          tttAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGTTATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTCATTAGCCCAGAACTTTAGCACCCACGCCCGCGCCACCGGt                                                                             >  2:4997173/1‑150 (MQ=255)
                                                                             aaaTATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTATTTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACg                                                                          <  1:4758722/150‑1 (MQ=255)
                                                                                  tGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGgccgc                                                                     <  2:5757113/150‑1 (MQ=255)
                                                                                  tGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGgccgc                                                                     <  2:3044776/150‑1 (MQ=255)
                                                                                   gAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGgccgcc                                                                    >  1:2345995/1‑150 (MQ=255)
                                                                                    aataatTTTTTCTTTCTGACAGTACGAATAATATATGCCGTAAACAAATGCAAAATGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAAGGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGCACGGCCGCCt                                                                   <  1:300623/150‑1 (MQ=255)
                                                                                    aataatTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCt                                                                   >  2:2357582/1‑150 (MQ=255)
                                                                                      taatTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTc                                                                 >  1:3138442/1‑150 (MQ=255)
                                                                                        atTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCAc                                                               >  1:2879300/1‑150 (MQ=255)
                                                                                         tttATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACg                                                              >  1:330061/1‑150 (MQ=255)
                                                                                          ttATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACgg                                                             <  1:4520294/150‑1 (MQ=255)
                                                                                            aTTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGAt                                                           <  1:1030403/150‑1 (MQ=255)
                                                                                             ttCGTTCTGACAGTACGAATAAGATATTCCGTCAAGAAATGAAATAAGGTCGCAGTAGCGCAATTTTCAATTCATTACTAATTACCGTGTAATTAGCCCAGAACTTTAGCAACAAGGCCCGCGCCTACGGTAGGGCCGACTTCACGGttt                                                          <  2:5996471/150‑1 (MQ=255)
                                                                                             ttCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGAtt                                                          >  1:2048910/1‑150 (MQ=255)
                                                                                              tCGTTCTTACATTACTATTAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTg                                                         <  2:6685830/150‑1 (MQ=255)
                                                                                              tCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTg                                                         <  2:4769690/150‑1 (MQ=255)
                                                                                               cGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGc                                                        <  1:4209323/150‑1 (MQ=255)
                                                                                                  tCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGaa                                                     <  2:2121834/150‑1 (MQ=255)
                                                                                                  tCTGACAGTACGAATAAGAAATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGaa                                                     >  2:5496244/1‑150 (MQ=255)
                                                                                                   cTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGaaa                                                    <  2:4636937/150‑1 (MQ=255)
                                                                                                   cTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGaaa                                                    <  2:4117488/150‑1 (MQ=255)
                                                                                                    tGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAAc                                                   >  1:1783492/1‑150 (MQ=255)
                                                                                                         gTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGa                                              <  1:5022516/150‑1 (MQ=255)
                                                                                                         gTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGa                                              >  2:1686017/1‑150 (MQ=255)
                                                                                                             gAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGACGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCCGAACTTTAGCAACAACGCCCGCGCCAACGGTCCGGCCGCCTTCACGGATTGCGAAACGCAGACcgt                                          >  1:3696407/1‑150 (MQ=255)
                                                                                                             gAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACcgt                                          >  1:7046548/1‑150 (MQ=255)
                                                                                                              aaTAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACcgtc                                         <  2:4809455/150‑1 (MQ=255)
                                                                                                                tAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACcgtcgt                                       >  2:3652148/1‑150 (MQ=255)
                                                                                                                tAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACcgtcgt                                       >  1:5900331/1‑150 (MQ=255)
                                                                                                                 aaGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGACACGCAGACCgtcgtc                                      >  2:5200392/1‑150 (MQ=255)
                                                                                                                    atatGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCAt                                   >  2:1916786/1‑150 (MQ=255)
                                                                                                                     tatGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATc                                  <  1:5070831/150‑1 (MQ=255)
                                                                                                                      atGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATcg                                 >  1:3505539/1‑150 (MQ=255)
                                                                                                                            tCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCCTCGCGAGCg                           >  1:5839224/1‑150 (MQ=255)
                                                                                                                            tCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCg                           <  1:4096813/150‑1 (MQ=255)
                                                                                                                            tCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCAGCGCGATCg                           >  2:1315616/1‑150 (MQ=255)
                                                                                                                             caacaaATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCgg                          <  2:1876059/150‑1 (MQ=255)
                                                                                                                              aacaaATGCAAAAAGGGCGCCGAAGCGCCCTGTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCACCGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGCTCggg                         >  2:627070/1‑150 (MQ=255)
                                                                                                                               acaaATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGt                        >  2:353044/1‑150 (MQ=255)
                                                                                                                                caaAGGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTg                       <  1:6828361/150‑1 (MQ=255)
                                                                                                                                 aaaTGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTgg                      >  2:6170791/1‑150 (MQ=255)
                                                                                                                                  aaTGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGa                     <  2:2456557/150‑1 (MQ=255)
                                                                                                                                      cAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAg                 >  2:7036400/1‑150 (MQ=255)
                                                                                                                                      cAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACAAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATAGCGAACGGGTGGATCAg                 >  1:4329064/1‑150 (MQ=255)
                                                                                                                                       aaaaaGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAgg                <  2:6319400/150‑1 (MQ=255)
                                                                                                                                        aaaaGGGCGCTTCGGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAggg               >  1:6587557/1‑150 (MQ=255)
                                                                                                                                         aaaGGGCGCTTCGGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGACACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGt              >  2:383131/1‑150 (MQ=18)
                                                                                                                                         aaaGGGCGCTTCGGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGt              >  2:5690269/1‑150 (MQ=255)
                                                                                                                                         aaaGGGCGCTTCGGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGt              >  1:1532378/1‑150 (MQ=255)
                                                                                                                                         aaaGGGCGCTTCGGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGt              >  1:748557/1‑150 (MQ=255)
                                                                                                                                           aGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTaa            <  2:4282791/150‑1 (MQ=255)
                                                                                                                                             ggCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCATACCGTCGTCCATCGCGATCGGGTGGATCAGGGTaaca          >  2:3158158/1‑150 (MQ=255)
                                                                                                                                               cgcCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTaacaac        >  2:6581620/1‑150 (MQ=255)
                                                                                                                                                gcCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTAACAAcc       >  1:1608394/1‑150 (MQ=255)
                                                                                                                                                gcCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTAACAAcc       >  2:335955/1‑150 (MQ=255)
                                                                                                                                                  cGAAGCGCCCTTTTCAATTCAAAACTAATTACCGTGTAATTAGCCCAGAACTTTAGCACCCACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTAACAACCAt     <  2:4902253/150‑1 (MQ=255)
                                                                                                                                                    aaGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTAACAACCAttt   <  2:1492409/150‑1 (MQ=255)
                                                                                                                                                    aaGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTAACAACCAttt   <  1:6908808/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
AGAAAAAAGCCTGACAAATGAAGCATTTTAAAAACAGAAACATTCATATTTAAAATGTTAAATTGAATTGATATTTTAAATATGAATAATTTATTCGTTCTGACAGTACGAATAAGATATGCCGTCAACAAATGCAAAAAGGGCGCCGAAGCGCCCTTTTCAATTCAAAACTAATTAACGTGTAATTAGCCCAGAACTTTAGCAACAACGCCCGCGCCAACGGTACGGCCGCCTTCACGGATTGCGAAACGCAGACCGTCGTCCATCGCGATCGGGTGGATCAGGGTAACAACCATTTT  >  NC_000913/3469960‑3470258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: