Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,504,855 T→G 5.7% intergenic (+72/+80) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,504,8550TG 5.7% 708.9 / 2.9 273intergenic (+72/+80)frlR/yhfSputative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base T (88/162);  new base G (9/14);  total (97/176)
Fisher's exact test for biased strand distribution p-value = 8.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 282 total aligned reads displayed.

GACAAACCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATG  >  NC_000913/3504706‑3505004
                                                                                                                                                     |                                                                                                                                                       
 aCAAACCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGtt                                                                                                                                                        >  1:2915632/1‑150 (MQ=255)
   aaaCCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACTTTcc                                                                                                                                                      >  1:5166526/1‑150 (MQ=255)
    aaCCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTccc                                                                                                                                                     <  2:2415949/150‑1 (MQ=255)
     aCCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTcccc                                                                                                                                                    <  2:2623374/150‑1 (MQ=255)
     aCCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTcccc                                                                                                                                                    >  1:1912830/1‑150 (MQ=255)
      ccGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGTATTTTACATCCCGTACGttggcct                                                                                                                                                   >  2:6155050/1‑145 (MQ=255)
        ggTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTgcccgcc                                                                                                                                                 >  2:6750132/1‑147 (MQ=255)
        ggTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCa                                                                                                                                                 <  1:754525/150‑1 (MQ=255)
        ggTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCa                                                                                                                                                 <  2:675087/150‑1 (MQ=255)
         gTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCacac                                                                                                                                                >  1:1616055/1‑150 (MQ=255)
         gTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAc                                                                                                                                                <  1:1592462/150‑1 (MQ=255)
          tGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTACCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCAcc                                                                                                                                               <  1:6266223/150‑1 (MQ=255)
             aCGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACAcccaa                                                                                                                                            >  2:2701386/1‑148 (MQ=255)
                 ctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAccc                                                                                                                                        <  1:4150448/150‑1 (MQ=255)
                    cGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCccct                                                                                                                                     <  1:4879755/150‑1 (MQ=255)
                    cGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGAGAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCctct                                                                                                                                     >  2:3594293/1‑150 (MQ=255)
                     gAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCtctc                                                                                                                                    <  1:4811032/150‑1 (MQ=255)
                     gAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGGTGAATTTTACATCCCGTACGGTCCCCTCACCCGAACCCtctc                                                                                                                                    >  2:3849180/1‑150 (MQ=255)
                      aaCTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTcc                                                                                                                                   <  1:903468/150‑1 (MQ=255)
                       aCTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTccc                                                                                                                                  <  2:6068599/150‑1 (MQ=255)
                       aCTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTccc                                                                                                                                  <  2:760970/150‑1 (MQ=255)
                          cttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGACCACACCCAAAACCAAACCCCaa                                                                                                                               >  1:3097307/1‑150 (MQ=38)
                           ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                              <  2:1246445/150‑1 (MQ=255)
                           ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCGAACCCACTCCCCcaa                                                                                                                              >  2:3984342/1‑150 (MQ=255)
                               gtcgcgCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTGCATCCCGTACGTTCCCCCCACCCTAACCCTCTCCCCACAgggg                                                                                                                          >  2:4983176/3‑150 (MQ=255)
                                ccGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGc                                                                                                                         <  2:154520/150‑1 (MQ=255)
                                   cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAg                                                                                                                      <  1:4383702/150‑1 (MQ=255)
                                   cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAg                                                                                                                      <  2:5037770/150‑1 (MQ=255)
                                    gcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAgg                                                                                                                     <  1:3627106/150‑1 (MQ=255)
                                    gcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAgg                                                                                                                     <  2:6018759/150‑1 (MQ=39)
                                     ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAggg                                                                                                                    <  2:4713722/150‑1 (MQ=255)
                                     ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAggg                                                                                                                    <  1:598957/150‑1 (MQ=39)
                                     ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCACACCCCAAAGGGGCGAggg                                                                                                                    >  2:2280677/1‑150 (MQ=255)
                                     caaaTCGCAACAACATAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAggg                                                                                                                    <  1:7223832/148‑1 (MQ=38)
                                      cAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAAACCACTACCCCAAGGGGGGAgggg                                                                                                                   >  1:4974904/1‑150 (MQ=38)
                                      cAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCTAACCCTCTCCCCAAAGGGGCGAgggg                                                                                                                   >  1:5447108/1‑150 (MQ=255)
                                                 ccTTAACTATTGATAATAAAAGACATTACCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                      <  2:4628358/150‑1 (MQ=255)
                                                 ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                      <  1:5659219/150‑1 (MQ=255)
                                                 ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                      >  2:5886799/1‑150 (MQ=255)
                                                   ttAACTATTGATAATAAAAGACATTAACCGTAGTCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTTCCCGCACACAAAAACCCACCCCCAACGGGCGAGGGGACAGCTCATTAcc                                                                                                    >  2:2481416/1‑149 (MQ=37)
                                                   ttAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACg                                                                                                    <  1:5038987/150‑1 (MQ=255)
                                                   ttAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAACCCACACCCCAAAGGGGCGAGGGGGCCGGTAAGTACg                                                                                                    >  2:6687863/1‑150 (MQ=38)
                                                     aaCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTAAATCCCGTACGTTACCCACACCCAAACCCACACCCCAAACGGGGGCGGGGGCCGGTCAGTAAGGa                                                                                                  >  1:3414453/1‑150 (MQ=37)
                                                      aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGaa                                                                                                 <  1:6936707/150‑1 (MQ=255)
                                                          ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTa                                                                                             <  2:6126097/150‑1 (MQ=255)
                                                          ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTa                                                                                             <  2:5537982/150‑1 (MQ=255)
                                                            gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATAGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCGCCCCCGAAACCCCAACCCCAACGGGCGGGGGGCCCGGTGCATACGGCATTTAGc                                                                                           >  1:5702520/1‑150 (MQ=25)
                                                             gtaataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTAAATCCCGTACGTTGCCCGCACCCAAAAACCCCCCCCAAAAGGGGGGGGGGGCCGGTGAGTGAGGAATTTATCt                                                                                          >  1:3217844/2‑150 (MQ=25)
                                                             ataataAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCCCACCCAAACCCACTCCCCCAACGGGCGAGGGGGCCCGTCAGTACGGCATTTATCt                                                                                          >  1:3806155/1‑150 (MQ=37)
                                                             ataataAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAACCCACACCCCAAAGGGGCGGGGGGGCCCGTCAGTAAGGGAAttttgt                                                                                           >  1:6515076/1‑147 (MQ=37)
                                                                ataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                       <  1:6095328/150‑1 (MQ=255)
                                                                  aaaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACg                                                                                     <  1:6750616/150‑1 (MQ=255)
                                                                   aaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACgg                                                                                    <  1:1735675/150‑1 (MQ=255)
                                                                     aGACATGAACCGTAGGCCGGATAAGATGCGCCAGCATCACATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                  <  2:4546434/150‑1 (MQ=255)
                                                                          ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACACCCCAAAGGGGCGACGGGACCGTTCCGTACGGAATTTAGCATGACGGTGCTATc                                                                             >  2:6554163/1‑150 (MQ=38)
                                                                          ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCAACCGGCGATGCTGGCGCGTTGAATTTTAAATCCCGTACGTTCCCCACACCCAAAACCACACCCCCAACGGGCGAGGGGGCCGTTCAGTACGGAAATTAGCGTTACGGTGCGAta                                                                             >  1:7213941/1‑149 (MQ=37)
                                                                           tAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATcc                                                                            <  1:4944121/150‑1 (MQ=255)
                                                                           tAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCACACCCAAACCCACAACCCCAACGGGCGGGGGGACCCGTACGTAACGGAATTAGCATTGCGGTGCTAtac                                                                            >  1:4786795/1‑148 (MQ=19)
                                                                             aCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAg                                                                          <  2:3436306/150‑1 (MQ=255)
                                                                                 tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAtt                                                                      <  2:1979879/150‑1 (MQ=255)
                                                                                    gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCCAACCCACAACCCCAAGGGGCGGGGGGACCGGTCAGTGAGGAATTTTGCGTTAACGTGCTATACAggcataaa                                                                   >  2:5557593/1‑143 (MQ=255)
                                                                                    gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACACTAAACCACAACCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCAATACAGCAATaaa                                                                   >  2:4364793/1‑150 (MQ=255)
                                                                                    gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATCCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTaaa                                                                   <  2:1898422/150‑1 (MQ=255)
                                                                                        gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCACTCCCCCAACGGGCGAGGGGCCCGTTCAGTACCGAATTTAGCGTGACGGGGCTATCCCGCAATAAAAAca                                                               >  2:6440115/1‑149 (MQ=255)
                                                                                        gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCACAACCGAACCCGCACCCCAAAGGGGGGAGGGGGCCCGTCAGTACCGCAATTATGTTGTCCGTGCTAAACAGCAATaaaaaaa                                                               >  2:2064504/1‑146 (MQ=255)
                                                                                         aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                              <  1:4792971/150‑1 (MQ=255)
                                                                                         aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                              <  1:1640660/150‑1 (MQ=255)
                                                                                         aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                              <  1:1396524/150‑1 (MQ=255)
                                                                                         aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                              <  1:61683/150‑1 (MQ=255)
                                                                                          tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                             <  2:1747868/150‑1 (MQ=255)
                                                                                          tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                             <  2:1384859/150‑1 (MQ=255)
                                                                                          tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                             <  1:3510358/150‑1 (MQ=255)
                                                                                          tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                             <  2:1912830/150‑1 (MQ=255)
                                                                                          tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                             <  2:5447108/150‑1 (MQ=255)
                                                                                           gtccatcgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGc                                                            <  2:7013918/144‑1 (MQ=255)
                                                                                             gATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCGAAACCTCTACCCCAATGGGCGGGGGGACCGGTCAGTACGGGCTTTATCATTACGGTGCTCTCCAGCATTAAAAACTGGCaa                                                          >  2:5624124/1‑150 (MQ=255)
                                                                                             gATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCAAACCCTAAAACACAACCCAAAAGGGCGAGGGGGCCGTTAAGTACCGAATTTAGCTTGGAGGTTCTATAAAGCATTAAAAACAGGCaa                                                          >  2:6210415/1‑150 (MQ=255)
                                                                                              aTGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCCTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAAt                                                         <  1:5362872/150‑1 (MQ=255)
                                                                                               tGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATa                                                        <  1:701671/150‑1 (MQ=255)
                                                                                                 cgcCAGCATCGCCGGATGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCGAACCCGCTACCCAAAGGGGCGAGGGGCCCGGTCAGTACGGAAATTAGCTTGTCGGTGCTAACCAGCATTAAATACTGGCAATaaa                                                      >  2:6253448/1‑148 (MQ=255)
                                                                                                  gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATActc                                                     <  2:220490/150‑1 (MQ=255)
                                                                                                     aGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCa                                                  <  1:1093768/150‑1 (MQ=255)
                                                                                                     aGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATACAGCATTAAATACTGGCAATACTCTCa                                                  >  2:4141929/1‑150 (MQ=255)
                                                                                                        atcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCGAACCCTCTCCCCACAGGGGCGAGGGGACCGGTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCACTACTCTCACGc                                               >  2:884745/1‑150 (MQ=255)
                                                                                                        atcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCATACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGGTCAGTACGGAAATTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCAccc                                               >  1:3926178/1‑148 (MQ=255)
                                                                                                               ccGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATcc                                        <  1:4671044/150‑1 (MQ=255)
                                                                                                                       gCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAcc                                <  1:229623/150‑1 (MQ=255)
                                                                                                                         tGGCGCGTAGAATTGTACATCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTATCTTGACGGTGCTATCCAGCATTAAATACGGGCAATACTCTCACGCAAAATCCGCAGCACCGt                              <  2:4916727/150‑1 (MQ=255)
                                                                                                                          ggCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCCCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGtc                             <  1:2559113/150‑1 (MQ=255)
                                                                                                                           gcgcGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGtct                            <  2:5702520/150‑1 (MQ=255)
                                                                                                                                 tGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCAc                      <  1:5886799/150‑1 (MQ=255)
                                                                                                                                     ttttACATCCCGTACGTTCCCCTCACCCAAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACCGAAATTAGCTTGACCGTGCTAACCAGCATTAAAAACTGGCAATACACTCACGCAAAATCCGCACCACCCTCACATCAccccg                  >  2:5287672/1‑147 (MQ=37)
                                                                                                                                       ttACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGc                <  1:4606174/150‑1 (MQ=255)
                                                                                                                                             cccGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCg          <  2:5159731/150‑1 (MQ=255)
                                                                                                                                              ccGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCgg         <  2:3217844/150‑1 (MQ=255)
                                                                                                                                              ccGTACGTTCCCCACACCCGAAACCTCTCCCCAAAGGGGCGCGGGGCCCGTTCAGTACGGAATTTAGCATGACGGTGCTATCCAGCATTAAAAACTGGCAATACTCTCACCCAAAATCCGCAGCACCGTCTCTTCACCGCAGCGATTAgg         >  2:2981532/1‑150 (MQ=255)
                                                                                                                                                gTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGt       >  2:4644165/1‑150 (MQ=255)
                                                                                                                                                 tACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGtt      <  1:1208524/150‑1 (MQ=255)
                                                                                                                                                   cGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGATCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTaa    <  1:935590/150‑1 (MQ=255)
                                                                                                                                                    gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt   <  1:6741470/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
GACAAACCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATG  >  NC_000913/3504706‑3505004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: