Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 114,506 G→T 5.2% intergenic (+19/+16) guaC → / ← hofC GMP reductase/assembly protein in type IV pilin biogenesis, transmembrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913114,5060GT 5.2% 426.1 / 8.4 142intergenic (+19/+16)guaC/hofCGMP reductase/assembly protein in type IV pilin biogenesis, transmembrane protein
Reads supporting (aligned to +/- strand):  ref base G (67/66);  new base T (4/5);  total (71/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

CGCGAGATATTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGCCAGCG  >  NC_000913/114357‑114655
                                                                                                                                                     |                                                                                                                                                     
cgcgAGATATTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTgg                                                                                                                                                       <  2:609099/150‑1 (MQ=255)
   gagaTATTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGc                                                                                                                                                    >  2:1730006/1‑150 (MQ=255)
    agaTATTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGTGCa                                                                                                                                                   >  1:1284963/1‑150 (MQ=255)
    agaTATTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCa                                                                                                                                                   <  2:1375494/150‑1 (MQ=255)
    agaTATTTTGGGCGGCCTGCGTTCAGCTTGTACATAAGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCa                                                                                                                                                   <  1:2042852/150‑1 (MQ=255)
      atatTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGCGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAac                                                                                                                                                 <  2:167084/150‑1 (MQ=255)
        atTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAacac                                                                                                                                               >  2:1858183/1‑150 (MQ=255)
          tttGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGc                                                                                                                                             <  2:67855/150‑1 (MQ=255)
           ttGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACTCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGcc                                                                                                                                            <  2:1075311/150‑1 (MQ=255)
            tGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCCACAAACTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCa                                                                                                                                           >  1:453710/1‑150 (MQ=255)
            tGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATATTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCa                                                                                                                                           <  2:865353/150‑1 (MQ=255)
             gggCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGTTGCTCCACGCCAg                                                                                                                                          <  2:1095014/150‑1 (MQ=255)
                 ggcCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGAAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGtt                                                                                                                                      <  1:716737/150‑1 (MQ=255)
                   ccTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTat                                                                                                                                    >  2:1951415/1‑150 (MQ=255)
                     tGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTatcc                                                                                                                                  >  1:1848930/1‑150 (MQ=255)
                      gCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTatccc                                                                                                                                 >  2:1797492/1‑150 (MQ=255)
                      gCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTatccc                                                                                                                                 >  2:1936035/1‑150 (MQ=255)
                        gTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGAGGAGCAACACGCCAGCGTTatccaat                                                                                                                               >  2:1274432/1‑150 (MQ=255)
                         ttCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTatcccatc                                                                                                                              <  1:1971532/150‑1 (MQ=255)
                          tCAGCTTGTACATACGTTGGGGCCTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTatcccatcc                                                                                                                             <  1:1129810/150‑1 (MQ=255)
                              cTTGTACATACGTTGGGGCTTCACGCCTGAAAGCGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGATATCCCATCCCACt                                                                                                                         >  1:1812495/1‑150 (MQ=255)
                               ttGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTc                                                                                                                        <  2:127246/150‑1 (MQ=255)
                               ttGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTc                                                                                                                        <  2:1686845/150‑1 (MQ=255)
                                tGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTca                                                                                                                       >  2:675128/1‑150 (MQ=255)
                                  tACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCGCGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTcatc                                                                                                                     <  2:2085779/150‑1 (MQ=255)
                                    cATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGATCAACACGCCAGAGTTATCCCATCCCACAcatcgc                                                                                                                   >  1:207717/1‑150 (MQ=255)
                                        cGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGTGTGGAGCAACACGCCAGCGTTATCCCATCCCACTcatcgcatcg                                                                                                               >  2:990784/1‑150 (MQ=255)
                                        cGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCCTAATCCCATCCCACTcctcgcatcc                                                                                                               >  1:99008/1‑149 (MQ=255)
                                          ttGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGcc                                                                                                             <  2:941447/150‑1 (MQ=255)
                                             gggCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAACACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGAGTGGAGCAACACGCCAGCGTTAACCCATCCAACTCATCGCATCGCCTaa                                                                                                          >  1:1357518/1‑150 (MQ=255)
                                               gCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAAt                                                                                                        <  2:1974992/150‑1 (MQ=255)
                                                cTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATg                                                                                                       <  1:224740/150‑1 (MQ=255)
                                                cTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATg                                                                                                       <  2:335144/150‑1 (MQ=255)
                                                     cGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGaaaa                                                                                                  >  2:953673/1‑150 (MQ=255)
                                                       ccTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAAtt                                                                                                <  2:1660292/150‑1 (MQ=255)
                                                        cTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGGGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTg                                                                                               <  2:1226049/150‑1 (MQ=255)
                                                          gAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCCGCGTTATCCCAGCCCACTCATCGCATCGCCTAAATGGAAAATTGGc                                                                                             >  1:655424/1‑150 (MQ=255)
                                                            aaGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAg                                                                                           <  2:2102753/150‑1 (MQ=255)
                                                            aaGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAg                                                                                           <  2:494779/150‑1 (MQ=255)
                                                                gCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCCACAACCTGTAATCTCCCAACGCTGGCGTGGAGCACCACGCCCGCGTTATCCCATCCCACGCATCGCAACGCCGAAATGGAAAATTGGCAGAGAc                                                                                       >  1:171968/1‑150 (MQ=255)
                                                                gCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATAc                                                                                       >  2:1180844/1‑150 (MQ=255)
                                                                   gACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACAtt                                                                                    >  1:1630673/1‑150 (MQ=255)
                                                                       aaGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGccc                                                                                >  1:1721553/1‑149 (MQ=255)
                                                                       aaGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGcca                                                                                >  2:770742/1‑150 (MQ=255)
                                                                            caccacGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGGCCGCGTTATCCGCTACCACTCATCGCATCGCCTAAATGGATAATTGGCAGCTGCATTGccgccacc                                                                           >  2:1509927/1‑150 (MQ=255)
                                                                             accacGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCATCAACAACCTGAAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCAACCCACTCATCGCATCTCCTAAATTTAATATTGGCAGATACATTGccaccacca                                                                          <  1:1562588/150‑1 (MQ=255)
                                                                                      aTTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCaa                                                                 >  2:540926/1‑150 (MQ=255)
                                                                                      aTTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTAGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCaa                                                                 >  2:1487128/1‑150 (MQ=255)
                                                                                        tCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCaata                                                               <  1:1397546/150‑1 (MQ=255)
                                                                                        tCGTGTGCAGGAACAAGAAAACCGCATCATCAACAACCAGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCaata                                                               <  2:203090/150‑1 (MQ=255)
                                                                                           tgtgCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGTGTGGAGCAACACGCCAGCGTTATCCCATCCCCCTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAAtt                                                            >  2:1851756/1‑150 (MQ=255)
                                                                                            gtgCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATtc                                                           <  2:325198/150‑1 (MQ=255)
                                                                                             tgCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATtcc                                                          >  2:2040313/1‑150 (MQ=255)
                                                                                              gCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCCCCACCAGCGTACCAATAATtcct                                                         >  1:2074009/1‑150 (MQ=255)
                                                                                               cAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATtcctc                                                        >  2:1550168/1‑150 (MQ=255)
                                                                                                 gggaCAAGAACACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCCTCCCCCTCAGCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTGCCCATAATTCCGccc                                                      >  1:1456493/4‑150 (MQ=255)
                                                                                                 ggAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGCGCAACACGCCAGCGTTATCCCATCCCACTCATCGCCTCGCCTAAATGGAAAATTGGCAGATACATTGCCACCGCCCGCGTCCCAATAATTCCGccc                                                      >  1:1265283/1‑150 (MQ=255)
                                                                                                 ggAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGGACCAATAATTCCTccc                                                      >  1:2095354/1‑150 (MQ=255)
                                                                                                     cAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTa                                                  >  1:499204/1‑150 (MQ=255)
                                                                                                       aGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGACACCACCAGCGTACCAATAATTCCTCCCGTTATg                                                >  1:577173/1‑150 (MQ=255)
                                                                                                          aaaCTGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATc                                             <  1:836555/150‑1 (MQ=255)
                                                                                                           acccGCATCTTCAACAACCTGAAATCTCCCAACGCGGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCa                                            <  2:301236/148‑1 (MQ=255)
                                                                                                           aaCCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCa                                            <  1:1045900/150‑1 (MQ=255)
                                                                                                           aaCCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCa                                            <  1:1684671/150‑1 (MQ=255)
                                                                                                           aaCCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCa                                            <  1:226253/150‑1 (MQ=255)
                                                                                                           aaCCGAATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCAAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCa                                            <  1:269930/150‑1 (MQ=255)
                                                                                                            aCCGCATCTACAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAg                                           <  2:100701/150‑1 (MQ=255)
                                                                                                             ccGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGc                                          <  2:1407301/150‑1 (MQ=255)
                                                                                                             ccGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGc                                          >  2:980340/1‑150 (MQ=255)
                                                                                                             ccGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGc                                          >  2:695478/1‑150 (MQ=255)
                                                                                                                  tCTTCAACACCCTGTAATCTCCCAACCCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCTCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACAAGAGTACCAATAATTCCTCCAGTTATTATCAGCAACGc                                     >  1:1290643/1‑150 (MQ=255)
                                                                                                                  tCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCACCGc                                     >  2:1719568/1‑150 (MQ=255)
                                                                                                                   cTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGcc                                    <  2:1776170/150‑1 (MQ=255)
                                                                                                                   cTTCAACAACCTGTAATCTCCCAACGCGGGCGTGGCGCAACACGCCAGCGTTATCCCATCCCACTCATGGCCTCGCCTAAATGGAAAAGTGGCAGATACATTGCCACCCCCCGCGGCCCAATAATTCCTCCCGGGAGGATCAGCACCGcc                                    >  1:1489557/1‑150 (MQ=255)
                                                                                                                      caacaaCCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCCCCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGt                                 >  1:922422/1‑150 (MQ=255)
                                                                                                                      caacaaCCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATACTTCCTCCCGTTATGATCAGCAACGCCGGt                                 >  2:436753/1‑150 (MQ=255)
                                                                                                                      caacaaCCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGt                                 <  2:747448/150‑1 (MQ=255)
                                                                                                                       aacaacCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGAAACATTGCCACAACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGtt                                >  1:1274761/1‑150 (MQ=255)
                                                                                                                         caacCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTcc                              <  2:1039152/150‑1 (MQ=255)
                                                                                                                           acattaaaTCTCCCAACGCTGGCGTGTAGCAACACTCCAGCTTTATCCCATCCCACTCATCGCATCGCCTAAATTGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAg                            <  1:333390/144‑1 (MQ=255)
                                                                                                                           acCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGTCGGTTCCAg                            <  1:1858183/150‑1 (MQ=255)
                                                                                                                             cTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTa                          <  1:410805/150‑1 (MQ=255)
                                                                                                                                 aaTCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGc                      >  1:1228817/1‑150 (MQ=255)
                                                                                                                                   tctcCCAACGCTGGCGTGGAGCAACACGCAAGCGTTATCCCATCCCACTCATCGCATCGCCTAACGGGAAAATTGGCAGGTACAATGCCCCAACCCGCGTACAAATAATGCCTCCGGTTATGATCAGCAACGCGGGTACAAGAAACGCTg                    >  1:1026343/1‑150 (MQ=255)
                                                                                                                                     tcCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCCCCAGCGTACCAAGAATTCCACCCGGTATGATCAGCAACGACGGTGCCAGTAAGGCTGcc                  >  2:925265/1‑149 (MQ=255)
                                                                                                                                     tcCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCg                  <  2:675485/150‑1 (MQ=255)
                                                                                                                                     tcCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATCGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGTTCAGAAACGCCGGATCCAGTAAGGCTGCg                  >  2:221851/1‑150 (MQ=255)
                                                                                                                                     tcCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACACATCGCAACGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCg                  >  1:1333836/1‑150 (MQ=255)
                                                                                                                                     tcCCAACGCTGGCGGGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCCTCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCg                  <  2:1218925/150‑1 (MQ=255)
                                                                                                                                        cAACGCTGGCGTGGAGCAACACGCCCGCGATATCCCATCCCACTCATCGCATCGACTAAATGGAAAATTGGCAGATACAGTGCCACCCCCAGCGTACCAATAATTCCTCCCGTTATGATCCGCGACGCCGGTTCCTGTAAGGCTGCGAgg               >  1:91125/1‑150 (MQ=255)
                                                                                                                                         aaCGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGt              <  2:99008/150‑1 (MQ=255)
                                                                                                                                          aCGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAACTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGtt             >  1:515448/1‑150 (MQ=255)
                                                                                                                                           cGCTGGCGTGGAGCAACACGCCAGCGATATCCCCTCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATAGCCACCACCAGCGTACCAAAAATTCCTCCCGTTATGATCAGCAACGCCGGATCCAGTAAGGCAGCGAGGTTa            >  2:8430/1‑150 (MQ=255)
                                                                                                                                             cTGGCGTGTTGCTCCACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTAta          >  1:989721/1‑149 (MQ=255)
                                                                                                                                             cTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATc          <  1:1256190/150‑1 (MQ=255)
                                                                                                                                             cTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCAACCCCTAAATGGAAAATTGGCAGATACCTTGCCACCACCAGCGTCCCAATAATTCCTCCCGTTATGATCAGCAACGCCGGGTCCAGGAAGGCAGCGAGGTTATc          >  2:389957/1‑150 (MQ=255)
                                                                                                                                               ggCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCg        <  2:1609910/150‑1 (MQ=255)
                                                                                                                                                   tGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGCCAg    >  2:750411/1‑150 (MQ=255)
                                                                                                                                                    ggAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGCCAgc   >  1:553338/1‑150 (MQ=255)
                                                                                                                                                     gAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGCCAgcg  >  1:1525195/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCGAGATATTTTGGGCGGCCTGCGTTCAGCTTGTACATACGTTGGGGCTTCACGCCTGAAAGAGCTGACCAAGCGCACCACGTTTATTCGTGTGCAGGAACAAGAAAACCGCATCTTCAACAACCTGTAATCTCCCAACGCTGGCGTGGAGCAACACGCCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGCCAGCG  >  NC_000913/114357‑114655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: