Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 464,358 T→G 5.7% intergenic (+50/‑44) ybaV → / → fadM putative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein/long‑chain acyl‑CoA thioesterase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913464,3580TG 5.7% 402.9 / 10.2 134intergenic (+50/‑44)ybaV/fadMputative competence‑suppressing periplasmic helix‑hairpin‑helix DNA‑binding protein/long‑chain acyl‑CoA thioesterase III
Reads supporting (aligned to +/- strand):  ref base T (63/63);  new base G (4/4);  total (67/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.29e-01

Only 100 of 135 total aligned reads displayed.

CGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTGGGATGGGTTG  >  NC_000913/464209‑464506
                                                                                                                                                     |                                                                                                                                                    
cgAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGt                                                                                                                                                      <  1:1250601/150‑1 (MQ=255)
  aaGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTa                                                                                                                                                    <  2:891383/150‑1 (MQ=255)
  aaGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGGGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTa                                                                                                                                                    <  2:507832/150‑1 (MQ=255)
   agagTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATATTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTAt                                                                                                                                                   >  1:1535535/1‑150 (MQ=255)
      gTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAAATAATTTGCATAGTCGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGAc                                                                                                                                                >  1:1681679/1‑150 (MQ=255)
       tACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGAcc                                                                                                                                               <  1:476811/150‑1 (MQ=255)
       tACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGAcc                                                                                                                                               <  2:667367/150‑1 (MQ=255)
          ggTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACctct                                                                                                                                            <  1:1422468/150‑1 (MQ=255)
           gTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAACTGGTATGACctctt                                                                                                                                           <  2:755134/150‑2 (MQ=255)
            tCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGt                                                                                                                                          >  1:1237610/1‑150 (MQ=255)
            tCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATAACTGGTATGACctcttc                                                                                                                                          <  2:775302/150‑3 (MQ=255)
              cGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTAc                                                                                                                                        <  1:1759101/150‑1 (MQ=255)
              cGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTAc                                                                                                                                        >  2:1183332/1‑150 (MQ=255)
               gTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATTACCTCTGTACt                                                                                                                                       >  1:1351317/1‑150 (MQ=255)
               gTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACt                                                                                                                                       >  1:212139/1‑150 (MQ=255)
               gTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACt                                                                                                                                       >  2:719096/1‑150 (MQ=255)
                 ttAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTta                                                                                                                                     <  1:921886/150‑1 (MQ=255)
                    aaaCTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATaa                                                                                                                                  >  2:553684/1‑150 (MQ=255)
                         gtgGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATaacaaca                                                                                                                             >  2:1190152/1‑150 (MQ=255)
                          gggaggaTCTAAAGCAGGTGGCGGGGATGGTCAATTAGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATaacaacaa                                                                                                                            <  2:1008320/149‑1 (MQ=255)
                           ggaggaTCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATaacaacaac                                                                                                                           >  1:1788128/1‑150 (MQ=255)
                           ggaggaTCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGAAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATaacaacaac                                                                                                                           >  1:1880224/1‑150 (MQ=255)
                            gaggaTCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACg                                                                                                                          <  2:1933755/150‑1 (MQ=255)
                             aggaTCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGt                                                                                                                         >  1:1063527/1‑150 (MQ=255)
                              ggggcTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCATGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGACATACGAGTTATGACCTCTGTACTTATAACAACAACGTa                                                                                                                        >  2:303449/5‑150 (MQ=255)
                                aTCTAAAGCAGGTGACGGGGATGGGCAATTCGCTGGTGGTACGTAATCTTGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGACAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAg                                                                                                                      <  1:854433/150‑1 (MQ=255)
                                  cTAAAGCAGGTGCCGGGGAAGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGt                                                                                                                    >  2:723757/1‑150 (MQ=255)
                                       gCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTg                                                                                                               <  2:1769850/150‑1 (MQ=255)
                                       gCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTg                                                                                                               >  1:1633366/1‑150 (MQ=255)
                                             gCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTAt                                                                                                         >  1:1227740/1‑150 (MQ=255)
                                             gCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAAAAACAACGTAAGGTTATTGCGCTAt                                                                                                         >  2:2041229/1‑150 (MQ=255)
                                             gCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACCGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTAt                                                                                                         >  2:1252805/1‑150 (MQ=255)
                                              ccGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGACATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATg                                                                                                        <  2:1839713/150‑1 (MQ=255)
                                               cGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGc                                                                                                       <  2:1726032/150‑1 (MQ=255)
                                               cGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGc                                                                                                       <  1:426881/150‑1 (MQ=255)
                                                ggggATGGTCAATTCGCTGGTGGAACGTAATCTGGCGGTAATAACCCTGTAATTAATTTTCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCa                                                                                                      <  1:1920573/150‑1 (MQ=255)
                                                   gatttgCAATTCTCTGGTGGAACGTAATCTGTCGTTATTAACACTGTAAATAATTTGCATATTTGCAATTTTTTTACAGACTGAAGATGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTTCGCTATGCAAac                                                                                                   <  2:1566388/145‑1 (MQ=255)
                                                     tGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAacac                                                                                                 >  1:1514463/1‑150 (MQ=255)
                                                       ggCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACaa                                                                                               >  2:1979130/1‑150 (MQ=255)
                                                        gCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGCCCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACcaa                                                                                              >  2:470429/1‑150 (MQ=255)
                                                         cAATTCGCTGGTGGAACGTAATCTGGCGGTATTTACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAAt                                                                                             <  2:1917990/150‑1 (MQ=255)
                                                          aaTTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATc                                                                                            <  1:1719325/150‑1 (MQ=255)
                                                          aaTTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAACAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATc                                                                                            <  2:1120018/150‑1 (MQ=255)
                                                              cGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAg                                                                                        >  2:362913/1‑150 (MQ=255)
                                                                cTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGtt                                                                                      <  1:1770653/150‑1 (MQ=255)
                                                                     ggAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTgg                                                                                 >  1:470884/1‑150 (MQ=255)
                                                                      gatagTAATCTGGCGGTATTAACACTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCATTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGa                                                                                <  1:299942/146‑1 (MQ=255)
                                                                        aCGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGata                                                                              <  1:695104/150‑1 (MQ=255)
                                                                             aTCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTTAAGAGGTAATACCAGTTATGACCTCTGTACTTATAACATCAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATatcatc                                                                         <  1:1079004/150‑1 (MQ=255)
                                                                             aTCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATatcatc                                                                         >  2:1958607/1‑150 (MQ=255)
                                                                              tCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCAtct                                                                        <  1:652157/150‑1 (MQ=255)
                                                                                 ggCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGa                                                                     >  2:1682962/1‑150 (MQ=255)
                                                                                   cGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACg                                                                   >  2:544893/1‑150 (MQ=255)
                                                                                   cGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACg                                                                   <  1:1313081/150‑1 (MQ=255)
                                                                                      tATTAAGCCTGTATTTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGttt                                                                <  1:1046700/150‑1 (MQ=255)
                                                                                      tATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGttt                                                                <  2:1445128/150‑1 (MQ=255)
                                                                                       tttAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGATGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACTACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTa                                                               <  2:1777242/149‑1 (MQ=255)
                                                                                       aTTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTa                                                               <  2:1514463/150‑1 (MQ=255)
                                                                                         tAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTAcc                                                             >  2:1981982/1‑150 (MQ=255)
                                                                                           aCCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAg                                                           <  1:1897921/150‑1 (MQ=255)
                                                                                            gccgggtatttatttTGATAGTGGGAATTTTTTGCCAGACTGAAGAGGTCATAACAGTTTTGAACTATGTTATTATAAAAACAACGTAATGTTTTTTGGGGGTGCAAACACAAATCAAAGTTGGGGGTTTTGATATCGGCGTTTTACAGc                                                          >  2:2032756/8‑150 (MQ=255)
                                                                                            cccTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGAGATCATCTCGACGTTTACCAGc                                                          >  2:1851296/1‑150 (MQ=255)
                                                                                               tGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACCGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACg                                                       <  1:1252805/150‑1 (MQ=255)
                                                                                                  aattaattTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACTCAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTca                                                    >  1:955617/1‑150 (MQ=255)
                                                                                                  aattaattTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTca                                                    <  1:809513/150‑1 (MQ=255)
                                                                                                           gCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGccc                                           >  2:2012095/1‑150 (MQ=255)
                                                                                                            cATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCg                                          <  1:1514842/150‑1 (MQ=255)
                                                                                                            cATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGAAAACACAAATCAAAGTTCGTGGATATCATATCGACGTTTACCAGCAAGTCAACACCGCCCg                                          >  2:2044886/1‑150 (MQ=255)
                                                                                                             ttAGTGGCAATTTTTTGCCAGACTGAAGAGGGCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCCTCTCGACGTTTACCAGCACGTCAACAACGCCCGc                                         <  2:2013521/149‑1 (MQ=255)
                                                                                                             aTAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGc                                         <  1:726797/150‑1 (MQ=255)
                                                                                                                gTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTAc                                      >  1:1202895/1‑150 (MQ=255)
                                                                                                                gTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTAc                                      >  1:1830840/1‑150 (MQ=255)
                                                                                                                  ggCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCCACAACGCCCGCTACCt                                    >  2:302909/1‑150 (MQ=255)
                                                                                                                  ggCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCt                                    <  1:204562/150‑1 (MQ=255)
                                                                                                                   gCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCtt                                   >  2:1175883/1‑150 (MQ=255)
                                                                                                                    cAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTg                                  <  1:616260/150‑1 (MQ=255)
                                                                                                                    cAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACAAGACCGTCAACAACGGCCGCTACCtta                                  >  2:478518/1‑149 (MQ=255)
                                                                                                                      aTTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGaa                                >  2:1430725/1‑150 (MQ=255)
                                                                                                                       ttttttGCCAGACTGAAGAGGTCATAGCAGTTATGTCCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGGAAACACAAATCAAAGTTCGTGGAAATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAAt                               <  2:1635158/150‑1 (MQ=255)
                                                                                                                            tGCCAGACTGAATAGTTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTtctc                          <  2:1705917/150‑1 (MQ=255)
                                                                                                                              ccAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCga                        <  1:395962/150‑1 (MQ=255)
                                                                                                                               cAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCgaa                       <  2:1788128/150‑1 (MQ=255)
                                                                                                                                aGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCATACACCAAGCAAAGTTCGAGGAAATCATCACGACGTTAACCAGCACGTCAACAACGCCCGCTACCTGTCAATTCTCgatg                      >  2:93653/1‑148 (MQ=255)
                                                                                                                                aGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCgaag                      >  1:1803639/1‑150 (MQ=255)
                                                                                                                                 aagtacagagGTCATAACTGGTATGACCTCTGTACTTATATCATCAACGTTAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTAGCAGCACGTCAACCACGCCCGCTACATTGAATTTCTCgaaga                     >  1:156065/7‑150 (MQ=255)
                                                                                                                                  aCTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCgaagaa                    <  1:1411159/150‑1 (MQ=255)
                                                                                                                                     gAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTAACAAGCACGTCAAAAACGCCCGCAAACTTGAATTTCTCGAAGAAGcc                 >  1:371606/1‑150 (MQ=255)
                                                                                                                                      cagagGTCATAACTGGTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGccc                >  1:1730474/2‑150 (MQ=255)
                                                                                                                                      cagagGTCATAACTGGTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTAGTGTATATAATCTCGAATTTTAACAGCAAGTCAACAACGACCGCTACCTAGAATTTCTCGAAGAAGccc                >  2:1253182/2‑150 (MQ=255)
                                                                                                                                      aaGAGGTCATACCAGTTATGACGTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGccc                >  1:1834933/1‑150 (MQ=255)
                                                                                                                                        gagGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCTTGGATATCATCTCGACTTTTACCAGCACGTCAACAACGCCCGCTACCTTCAATTTCTCGAAGAAGCCCGc              >  1:323695/1‑150 (MQ=255)
                                                                                                                                        gagGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTACGCAAACACAAATCAACGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGc              <  1:315355/150‑1 (MQ=255)
                                                                                                                                        gagGTCATAACTGGTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGc              >  2:1333876/1‑150 (MQ=255)
                                                                                                                                             cATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTGGGa         >  2:1206249/1‑150 (MQ=255)
                                                                                                                                             cATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTGGGa         <  1:719096/150‑1 (MQ=255)
                                                                                                                                               tACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTGGGATg       <  2:1633366/150‑1 (MQ=255)
                                                                                                                                               tACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTGGGATg       <  1:2093642/150‑1 (MQ=255)
                                                                                                                                                   aGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTGGGATGGGtt   >  2:1593408/1‑150 (MQ=255)
                                                                                                                                                    gTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTGGGATGGGTTg  >  2:798430/1‑150 (MQ=255)
                                                                                                                                                    gTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTGTACCAGCACGTCAACAACGCCCGCTACCTTGAAGTTCTCGAAGAAGCCCGAGGGGATGGGTTg  >  2:82345/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGAAGAGTACGGTCCGTTTAAAACTGTGGAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTATTAACCCTGTAATTAATTTGCATAGTGGCAATTTTTTGCCAGACTGAAGAGGTCATACCAGTTATGACCTCTGTACTTATAACAACAACGTAAGGTTATTGCGCTATGCAAACACAAATCAAAGTTCGTGGATATCATCTCGACGTTTACCAGCACGTCAACAACGCCCGCTACCTTGAATTTCTCGAAGAAGCCCGCTGGGATGGGTTG  >  NC_000913/464209‑464506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: