Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 756,150 C→T 100% Q82* (CAG→TAG)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,1500CT97.2% 464.1 / 0.7 141Q82* (CAG→TAG) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base T (59/77);  total (62/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 141 total aligned reads displayed.

TGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAG  >  NC_000913/756003‑756293
                                                                                                                                                   |                                                                                                                                               
tgtgCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAg                                                                                                                                               <  1:858846/150‑1 (MQ=255)
 gtgCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACCCCGTGAAAGGGTCGGACTATATCGGTGACCAgg                                                                                                                                              >  2:595393/1‑150 (MQ=255)
     gcggctCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGAcgc                                                                                                                                          <  2:1500923/147‑1 (MQ=255)
      ctactctctAAAGTCTTCCCGACCCGTTCCCATTCCGTTTCTGCGCAAGGCGGCATTACCGTTTCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGAGTAGGAcgcg                                                                                                                                         >  2:231225/4‑150 (MQ=255)
        gctCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGAt                                                                                                                                       >  1:945042/1‑150 (MQ=255)
        gctCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGAt                                                                                                                                       >  2:1632743/1‑150 (MQ=255)
        gctCTCTAAAGTCTTCCCGACCCGGTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGAt                                                                                                                                       <  2:1652090/150‑1 (MQ=255)
         ctctctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTTCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGACGATAACTGGGAATTGCATATGTACGACACCGTGAAAGGGGCGGACTATATCGGTGACTAGGACGCGAtt                                                                                                                                      <  1:591352/150‑1 (MQ=255)
         ctctctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGAtt                                                                                                                                      <  1:1716802/150‑1 (MQ=255)
            tctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGaa                                                                                                                                   >  2:607751/1‑150 (MQ=255)
            tctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGaa                                                                                                                                   <  1:928882/150‑1 (MQ=255)
            tctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGaa                                                                                                                                   >  1:1170032/1‑150 (MQ=255)
              tAAAGTCTTCCCGACCCGTTCCCATACCGTTGCTGCGCAAGTCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAata                                                                                                                                 <  2:329923/150‑1 (MQ=255)
               aaaGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGAAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAatat                                                                                                                                <  2:363923/150‑1 (MQ=255)
                aaGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAatata                                                                                                                               <  1:30850/150‑1 (MQ=255)
                aaGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCCTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAatata                                                                                                                               >  2:1596233/1‑150 (MQ=255)
                  gTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATAtg                                                                                                                             <  1:1449343/150‑1 (MQ=255)
                  gTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGACATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATAtg                                                                                                                             <  2:327427/150‑1 (MQ=255)
                   tattCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATAtgt                                                                                                                            <  2:1762808/148‑1 (MQ=255)
                            cccGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCg                                                                                                                   >  1:1516068/1‑150 (MQ=255)
                            cccGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACGGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTAGATCGGTGACTAGGACGCGATTGAATATATGTGTAACACCg                                                                                                                   >  2:1121465/1‑150 (MQ=255)
                              cGTTCCCATACAGTTTCTGCGCAAGGCGGCATTACCGTTGCGATGGGTAATACAAATTATGATAAATGGGAAAGCCATATGTACGACTCAGTGAAACTGTCGGACTATATCGGAGACAAGGACGCGAATCAATATATTTGTAAAAcacgc                                                                                                                 >  2:1415891/1‑146 (MQ=255)
                                   ccATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGa                                                                                                            >  2:937082/1‑150 (MQ=255)
                                     aTACCGTTGCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAg                                                                                                          <  2:286482/150‑1 (MQ=255)
                                      tACCGTGTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGTCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGc                                                                                                         <  2:1081544/150‑1 (MQ=255)
                                        ccGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGa                                                                                                       >  2:51265/1‑150 (MQ=255)
                                         ggTTTCAGCGCAAGGCGGCAATACGGGAGCGCGGGGTAATACCCATGAAGATTACGGGGAATGGCATATGTACGACACCGTGAAAGGGGCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGAt                                                                                                      <  1:1025674/149‑1 (MQ=255)
                                            ttCTGCGCAAGGCGGCTTTACCGTTGCGCTGGGTAATACCCATGAATATAACTGGGAATGGCATATGTACGACACCGTGAAATGGTCTGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCt                                                                                                   <  2:9799/150‑1 (MQ=255)
                                             tCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTg                                                                                                  <  2:1176964/150‑1 (MQ=255)
                                             tCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTg                                                                                                  <  2:1920602/150‑1 (MQ=255)
                                              cTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGATAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTgg                                                                                                 <  2:522122/150‑1 (MQ=255)
                                                 cgcAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTATAACCGGGCCGGAAGCGATTCTGGAAc                                                                                              <  1:136428/150‑1 (MQ=255)
                                                 cgcAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAAc                                                                                              <  1:1662708/150‑1 (MQ=255)
                                                 cgcAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAAc                                                                                              <  2:1279901/150‑1 (MQ=255)
                                                    aaGGCTGCATTACCGTTGCGCTGTGTAATACCCATGAAGATAACTGTGAATGGCATATGTACGACACCGTTAAAGGGTCGGACTATATAGGTGACTTGGACGCGATTGAATATATTTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCg                                                                                           <  1:1248082/150‑1 (MQ=255)
                                                     aGGCGGCATTACCGTTGCGCTTGGTAATACCCATGAAGATAACTGGGAATGGCATATCTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGa                                                                                          <  2:839429/150‑1 (MQ=255)
                                                     aGGCGGCATTACCGGTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGa                                                                                          <  2:1462224/150‑1 (MQ=255)
                                                      ggcggcATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGaa                                                                                         >  2:220336/1‑150 (MQ=255)
                                                       gcggcATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAac                                                                                        >  2:2045811/1‑150 (MQ=255)
                                                        cggcATTACCGTTTAGCTTGGTAATACCCATGTAGATAACTGGGAATGGCATATGTACGACAACTTGAAAGGGTCGGACTATATCTGTGTCTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAaca                                                                                       <  1:1314703/150‑1 (MQ=255)
                                                              tACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATGGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATggg                                                                                 <  1:1448333/150‑1 (MQ=255)
                                                               atcGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGCCTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGc                                                                                >  2:1357821/3‑150 (MQ=255)
                                                               aCCGTTTCCCGGGGTAATACCCATGAATATAACTGGGAATGTGATATGGACGAAACCGTGAAATGGTCTGACTATATCGGTGACTAGGACGCGATAGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGc                                                                                <  1:157500/150‑1 (MQ=255)
                                                               aCCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGc                                                                                >  1:1420061/1‑150 (MQ=255)
                                                                ccGTTGCGCTGTGTAATACCCATGAAGATAACTGGGAAGGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGcc                                                                               <  1:2013928/150‑1 (MQ=255)
                                                                ccGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGcc                                                                               <  1:622314/150‑1 (MQ=255)
                                                                  gTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTg                                                                             >  2:1638981/1‑150 (MQ=255)
                                                                   ttGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGc                                                                            <  1:1365236/150‑1 (MQ=255)
                                                                     gcgcTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCg                                                                          <  2:716271/150‑1 (MQ=255)
                                                                         tGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGTGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGTAAGCGATTCTGTAACTCGAACACATGGGCCTGCCGTtct                                                                      <  1:1588978/150‑1 (MQ=255)
                                                                           ggTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTcg                                                                    >  1:1800713/1‑150 (MQ=255)
                                                                           ggTAATACATATGAAGTTAAGTGTGATTTTCATATGTACGACAGCGTGATAGGGGCGGACTATATCGGTGACTAGGACGCTATTGTATATATGTGTATAACCGGGCCGGATGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTcg                                                                    <  1:816769/150‑1 (MQ=255)
                                                                                  cccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCg                                                             >  1:531841/1‑150 (MQ=255)
                                                                                   ccATGAAGATAACTGGGAATGGCATATGAACGACACCGTGAAAGGGGCGGCCTATATCGGTGACTAGGCCGCGATTGAATATATGTGTAAAACCGGCCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCga                                                            <  1:1157888/150‑1 (MQ=255)
                                                                                    cATGAAGATAACAGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACAATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGAATCTGGAACTCGAACACATGGGCCTTCCGTTCTCGCGTCACgat                                                           >  1:900768/1‑150 (MQ=255)
                                                                                        aaGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTAGAACACATGGGCCTGCCGTTCTCGCGTCTCGatgatg                                                       >  1:1100168/1‑150 (MQ=255)
                                                                                         ctatatCTGTGAATTTCATATGTTCGCAACTGTGAAAGGGTTGGACTATATCGGTTTGTAGGAAGAGATTGAATATATGTGTAATTCCGGGCCGGAATCGTTTCTGGACCTCGAACACTTGGGCCTTCCGTTCTCGCGTCTCGATGCTgg                                                      <  2:181658/148‑1 (MQ=255)
                                                                                         aGAAAACTCGGAATGGCATAGCGACGACACCGTGAACGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAACCCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATgg                                                      <  1:1259015/150‑1 (MQ=255)
                                                                                            tAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCg                                                   <  1:1309933/150‑1 (MQ=255)
                                                                                            tAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCg                                                   <  2:669040/150‑1 (MQ=255)
                                                                                            tAACTGGGAATGGCATATGTACGACACCGTGAAAGGGGCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAAGCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCg                                                   <  1:177339/150‑1 (MQ=255)
                                                                                             aaCTGGGAATGGCATATGTACGACAACGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCTTTCTCGCGTCGCGATGATGGTCGt                                                  >  2:1307237/1‑150 (MQ=255)
                                                                                              aCTGGGAATGGCATATGTACGACACCGTGAACGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGTAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGGCGta                                                 <  2:2000903/150‑1 (MQ=255)
                                                                                                tGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatc                                               >  1:2074124/1‑150 (MQ=255)
                                                                                                 gggAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGACCACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatct                                              >  2:1506565/1‑150 (MQ=255)
                                                                                                 gggAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatct                                              >  2:128353/1‑150 (MQ=255)
                                                                                                    aaTGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatctatc                                           <  1:715256/150‑1 (MQ=255)
                                                                                                       ggCATATGTACGACACCGTGAATGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAAc                                        <  2:658525/150‑1 (MQ=255)
                                                                                                        gCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACg                                       >  2:554740/1‑150 (MQ=255)
                                                                                                         cATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGt                                      >  1:1733074/1‑150 (MQ=255)
                                                                                                          atatGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTc                                     <  1:1030355/150‑1 (MQ=255)
                                                                                                             tGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGt                                  >  2:803070/1‑150 (MQ=255)
                                                                                                             tGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGt                                  >  1:1239410/1‑150 (MQ=255)
                                                                                                             tGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGt                                  >  1:1127021/1‑150 (MQ=255)
                                                                                                              ttACGACAGCGTGTAAGGGTCGGACTATTTGGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGAGGGTCGTATCTATCAACGTCCGtt                                 <  1:380823/149‑1 (MQ=255)
                                                                                                              gTACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAAAATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCTTATCTATCAACGTCCGtt                                 >  1:1030027/1‑150 (MQ=255)
                                                                                                               tACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGttt                                <  1:1722507/150‑1 (MQ=255)
                                                                                                               tACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGttt                                >  1:443605/1‑150 (MQ=255)
                                                                                                               tACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGGTCTCGCGGCTCGATGATGGTCGTATCTATCAACGTCCGttt                                >  2:1346373/1‑150 (MQ=255)
                                                                                                               tACGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGACGGAAGCGATTCAGGAACTCGAACACATGGTCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGACCgata                                >  2:380823/1‑147 (MQ=255)
                                                                                                               tACGACACCGTGAAAGGGTCGGACTATATCGGGGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGttt                                <  1:861355/150‑1 (MQ=255)
                                                                                                                 cGACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGGTTgg                              >  1:1535448/1‑150 (MQ=255)
                                                                                                                  gACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGc                             <  1:1164350/150‑1 (MQ=255)
                                                                                                                  gACACCGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGc                             <  1:51265/150‑1 (MQ=255)
                                                                                                                      ccGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTc                         >  1:192850/1‑150 (MQ=255)
                                                                                                                      ccGTGAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGATTGGCGGTc                         >  1:1574000/1‑150 (MQ=255)
                                                                                                                          gAAAGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGGAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGGTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTc                     <  1:882818/150‑1 (MQ=255)
                                                                                                                          gAAAGCGGCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAATCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTc                     <  2:1594536/150‑1 (MQ=255)
                                                                                                                            aaGGGTCGGACTAAATCGGGGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTGCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGGCGa                   <  1:749306/150‑1 (MQ=255)
                                                                                                                             aGGGTCGGACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTATGGCGGTCAGTCGaa                  >  2:2053273/1‑150 (MQ=255)
                                                                                                                              gggTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGaaa                 <  1:1887754/150‑1 (MQ=255)
                                                                                                                                   ggACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGTGGTCAGTCGAAAAACtt            >  2:1211491/1‑150 (MQ=255)
                                                                                                                                    gACTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTc           >  1:1965598/1‑150 (MQ=255)
                                                                                                                                    gACTATATCGGGGACTAGGACGCTATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTc           <  2:2006544/150‑1 (MQ=255)
                                                                                                                                      gtataTCGCTGACTAGGACGCGATTGAATATATGTGTAACACCGGGCCGGAAGCGATTCTGTATCTCGAACACATGGGCCTGCCGGTCTCGCGTCTCGATGAGGGTCGTATCTATCAACGTCCCTTTGGCGGTCAGTCGAAAAACTTcgg         <  1:87232/149‑1 (MQ=255)
                                                                                                                                      cTATATCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACACGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcgg         <  2:1398043/150‑1 (MQ=255)
                                                                                                                                      cTATATCGGGGACTAGGCCGCGATTGAATATATGTGTAAACCCGGGCCGGAAGCGCATCTGGAACTCGACCACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGGCGTATCTATCAACGTCCGGTTGGCGGTCAGTCGAAAAACTTcgg         <  1:1121465/150‑1 (MQ=255)
                                                                                                                                        atatCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcg       >  1:1552790/1‑150 (MQ=255)
                                                                                                                                           tCGGTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGgcggcg    <  1:1065410/150‑1 (MQ=255)
                                                                                                                                             ggTGACTAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAg  >  2:1067660/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAG  >  NC_000913/756003‑756293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: