Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 857,576 T→C 6.0% intergenic (+21/+220) ybiT → / ← ybiU ABC‑F family putative regulatory ATPase/DUF1479 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913857,5760TC 6.0% 322.8 / 6.0 117intergenic (+21/+220)ybiT/ybiUABC‑F family putative regulatory ATPase/DUF1479 family protein
Reads supporting (aligned to +/- strand):  ref base T (51/57);  new base C (4/3);  total (55/62)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

TTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACAAAG  >  NC_000913/857435‑857723
                                                                                                                                             |                                                                                                                                                   
ttCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCgg                                                                                                                                             >  1:1447118/1‑150 (MQ=255)
 tCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCggg                                                                                                                                            >  1:72903/1‑150 (MQ=255)
 tCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCggg                                                                                                                                            <  1:5805/150‑1 (MQ=255)
 tCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCggg                                                                                                                                            >  1:546060/1‑150 (MQ=255)
 tCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCAGTCACCCCGATACTGTCggg                                                                                                                                            >  2:12621/1‑150 (MQ=255)
    cacGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTg                                                                                                                                         >  1:2081733/1‑150 (MQ=255)
      cGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGAc                                                                                                                                       <  2:895474/150‑1 (MQ=255)
      cGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGACAGTATCGGGGTGAc                                                                                                                                       <  2:1566551/150‑1 (MQ=255)
        aCCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGGCGGGGTGAcgg                                                                                                                                     <  2:2022892/150‑1 (MQ=255)
         ccGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAACTCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGAcggc                                                                                                                                    <  1:656420/150‑1 (MQ=255)
            tttgTTCGTAAGCTCCCTGGCGACCCTCTTTTTTTAAATCACCCGGTACGGGGTTATCTATTTTTGCGTTATTTACTAATTTAACCTGCGTATTAATGGGATGGTTTATGTTATTATCCTTCACCCCTATACTGTCGGGGTGACggcggc                                                                                                                                 <  2:448813/147‑1 (MQ=255)
            ggAGTACGTAAGCTCCCTCGCGACCCGCATTCTGGAAATCACCCCGGAACGCGGGATCCACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACggcggc                                                                                                                                 <  1:1263181/149‑1 (MQ=255)
              aGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCACGATACTGTCGGGGTGACGGCGGCtg                                                                                                                               >  2:1885789/1‑150 (MQ=255)
              aGTTCGTAAGCGCCCTGGCGACCCGCATTATGTAAATCACCCCGGAAACCGTTATCGACTTTAGCGGTAATTACGAATAATACCTGCGTAGTAAAGGGATCGAGTAAGTTATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtg                                                                                                                               <  2:1130066/150‑1 (MQ=255)
               gTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtga                                                                                                                              >  1:417989/1‑150 (MQ=255)
                tgcGTAAGCTCCCTGGCGACCCGCATTCTGGAAAGCCCCCCGGAACGCGTGATCGACTTTCGCGGTAATTCCGAATATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtgat                                                                                                                             <  2:345580/148‑1 (MQ=255)
                  cGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtgatga                                                                                                                           <  1:1437054/150‑1 (MQ=255)
                  cGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtgatga                                                                                                                           >  2:1266870/1‑150 (MQ=255)
                    tAAGCTCCCTGGCGACCCGAATTCTGGAAATCACCCCGTAACGCGTGATCGACTTTAGCGTTAATTACGAAGATTACCTGCGTTGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCTATACTGTCGGGGTGACGGCGGCTGATGACa                                                                                                                         <  2:1175630/150‑1 (MQ=255)
                        cTCCCTCGCGACCCGCATTCTGGAAATCACCCCGGAACGCCTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATg                                                                                                                     <  2:49201/150‑1 (MQ=255)
                          cccTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCa                                                                                                                   <  2:1129/150‑1 (MQ=255)
                          cccTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCa                                                                                                                   >  1:511510/1‑150 (MQ=255)
                            cTGGGGACCCGCATTCTGTAAATCACCCGGGAACGCGTGATCGACTTAAGCGGTAATTCCGAAGATTACCTGCGTTGTTATGGGATCGATTAAGTGATTATCCGTCACCCCTTTACTGTCGGGGTGACGGCGGCTGATGACAAATGCaaa                                                                                                                 <  1:400165/150‑1 (MQ=255)
                            cTGGCGACCCGCATTCTGGAAATCCCCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCaaa                                                                                                                 <  1:242706/150‑1 (MQ=255)
                             tGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGAATTTAGCGGTAATTACGAAGATAACCTGCGTAGTAATGGGATCGAGTAAGTGATTATCCGTCACCCCTATACTGTCGGGGTGACGGCGGCTGATGACAAATGCaaaa                                                                                                                <  2:511199/150‑1 (MQ=255)
                                ccaCCCGCATTCTGCAAATCACCCCGGACCCCGTGATCGCCTTTAGCGGTAATTACGACGAATACCGGCGTAGAAAAGGGATCGATTAAGTGATTCTCCGTCACCCCGATACTGGCGGGGTGACGGCGGCTGATGACAAATGCAAAATAg                                                                                                             <  2:1283038/148‑1 (MQ=255)
                                   cccGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGGAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCt                                                                                                          >  1:875835/1‑150 (MQ=255)
                                   cccGCATTCTGGAAATAACCCCGGAACGCGTGAGCGACTTTGGCGGTAATTACTAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTTATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCt                                                                                                          <  1:167074/150‑1 (MQ=255)
                                    ccGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTg                                                                                                         >  2:1115969/1‑150 (MQ=255)
                                           cTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCt                                                                                                  >  1:1252052/1‑150 (MQ=255)
                                            tGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTa                                                                                                 >  2:859590/1‑150 (MQ=255)
                                               aaaTCACCCCGGACCGCGTCATCGACTTTACCGGTAATTACGAATATTACCTGCGTAGTAACGGGATCGAGTAAGTGATTATCCGTCACCCCGACACTGGCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGc                                                                                              <  1:174791/150‑1 (MQ=255)
                                               aaaTCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGc                                                                                              <  1:764406/150‑1 (MQ=255)
                                                  tCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTa                                                                                           >  1:65258/1‑150 (MQ=255)
                                                    aCCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATc                                                                                         >  1:139559/1‑150 (MQ=255)
                                                     ccccGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCa                                                                                        >  2:1398605/1‑150 (MQ=255)
                                                     ccccGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCa                                                                                        >  2:1834129/1‑150 (MQ=255)
                                                     ccccGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGAAGCGCTACGCTTATCa                                                                                        >  2:652948/1‑150 (MQ=255)
                                                        cGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGc                                                                                     <  1:1392507/150‑1 (MQ=255)
                                                         ggAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGcc                                                                                    >  2:189473/1‑150 (MQ=255)
                                                             cgcgTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACg                                                                                >  2:1772984/1‑150 (MQ=255)
                                                              gcgTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGc                                                                               >  1:2103473/1‑150 (MQ=255)
                                                               cgTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCa                                                                              >  2:1523923/1‑150 (MQ=255)
                                                                gTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAg                                                                             >  1:1331511/1‑150 (MQ=255)
                                                                        cTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCa                                                                     <  2:776834/150‑1 (MQ=255)
                                                                          ttcgCGGTAATACCTACGATTACCTGGGTAGAAAAGGGATCGAGAAAGTGATTAACCGCCACCCCGACCCTGGCGGGGGGAGGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAat                                                                   <  1:1644220/147‑1 (MQ=25)
                                                                             gCGGTAATTATGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCCGGCCTACGCAGTACCTGCAatata                                                                >  2:1896804/1‑150 (MQ=255)
                                                                             gCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAatata                                                                >  1:1315724/1‑150 (MQ=255)
                                                                             gCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAatata                                                                >  1:2057516/1‑150 (MQ=255)
                                                                               ggTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATAtt                                                              <  2:1935070/150‑1 (MQ=255)
                                                                                ttAATTACTAATATTACCTGCGTAGTAAAGGCATCGTGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTg                                                             <  1:808590/149‑1 (MQ=255)
                                                                                ttAATTACTAAGTTTACCTGCTTATTAAAGGGATCGTTTAAGTGATTATCCGTCACCCCGATAGTGTCGGGGTGAGGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTg                                                             <  2:750725/149‑1 (MQ=37)
                                                                                gTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTg                                                             <  2:48730/150‑1 (MQ=255)
                                                                                  aaTTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCTATATATTGaa                                                           >  1:330376/1‑150 (MQ=255)
                                                                                    ttACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTAACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATc                                                         <  2:2012094/150‑1 (MQ=255)
                                                                                     tACGCAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGCCGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTTCCTGCAATATATTGAATCt                                                        >  2:93560/1‑150 (MQ=255)
                                                                                     tACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGCTGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATcg                                                        >  2:2048275/1‑149 (MQ=255)
                                                                                      aCGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTg                                                       >  2:2008821/1‑150 (MQ=255)
                                                                                       cGAAGATTACCGGGGTAGTAAGGGGAGCGTGTATGTGATTTTCCTTCACCCCGATAGTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGc                                                      <  2:735331/150‑1 (MQ=37)
                                                                                        caaGTTTCCCTGCGTAGTAACGGGAGCGAGTAAGTTATTACCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCa                                                     <  1:676328/149‑1 (MQ=37)
                                                                                          aGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAgg                                                   <  2:467467/150‑1 (MQ=255)
                                                                                            aTTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGAc                                                 <  2:1338170/150‑1 (MQ=255)
                                                                                            aTTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGAc                                                 <  2:825298/150‑1 (MQ=255)
                                                                                                ccTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACtttt                                             >  2:1162718/1‑149 (MQ=255)
                                                                                                 gtgtgtattaATGGGATCTTTTATGTTATTATCGGACACCCCTTTAGTGGGGGGGTGAGGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGt                                            <  2:712961/146‑1 (MQ=21)
                                                                                                  tGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACTCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTa                                           >  1:1789365/1‑150 (MQ=255)
                                                                                                  tGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTa                                           <  1:1252242/150‑1 (MQ=255)
                                                                                                  tGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGATGATGACAAATGCAAAATATCCTGATGCGCTACTCTTATCAGGCCTACGCAGTACCTGAAATATATTGAATCTGCAGGAATTTGTa                                           >  1:1780949/1‑150 (MQ=255)
                                                                                                    cGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGAATGACTTTTTAgg                                         >  2:1409293/1‑150 (MQ=255)
                                                                                                       tttaATGGGCTCGAGTAAGTGATTTTCCTTCACCCCGATACTGTCGGGGTTACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCg                                      <  2:1886471/148‑1 (MQ=37)
                                                                                                        gtaAAGGTATCTTGTAAGTGATTATCCGTCCCCCCTATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCgg                                     <  2:2081733/150‑1 (MQ=37)
                                                                                                        gtaAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCgg                                     <  1:262804/150‑1 (MQ=255)
                                                                                                        gtaAAGGCTTCGTGTACGTGATTATCCGGCCCCCCTATACTGCCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCgg                                     <  1:971595/150‑1 (MQ=25)
                                                                                                         tacaGGGATCGAGTAAGTGATTATCCGTCTCCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGa                                    <  2:240662/147‑1 (MQ=255)
                                                                                                         aaaaGTGGTCGAGTATGTGATTCTCCGGCTCCCCCATATTTTCGGGGTGACGGCGGCTGATGACAAATGCAAATTAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGa                                    <  2:1032350/149‑1 (MQ=25)
                                                                                                           aaGGCATCGAGTAATTGATTATCCGGCCCCCCGATACTGTCGGGGTGACGGCGGCTGATGACACATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATa                                  <  2:365195/150‑1 (MQ=37)
                                                                                                              ggATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAgg                               >  1:1338737/1‑150 (MQ=255)
                                                                                                              ggAGCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGTCTGATGACAAATGCAAAATAGCCTGTTGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAgg                               <  2:1094265/150‑1 (MQ=255)
                                                                                                                  cGAGTAAGTGATTATCCTTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGtt                           <  2:1075404/150‑1 (MQ=255)
                                                                                                                   gAGTAAGTGATTATCCGTCACCCCGTTACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAAAATCCTGATGCTCTACGCTTATTAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTTGGCCGGATAAGGCGttt                          <  2:1375541/150‑2 (MQ=39)
                                                                                                                       aaGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGAGGCGGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgc                      >  1:588417/1‑150 (MQ=255)
                                                                                                                       aaGTGATTATCCGGCACCCCGATACTGTCGTGGTGACGGCGGCTGATGACAAATGCAAAATCGCCTGATGCGCGACGCTGATCAGGCCTACGCAGAACCTCCAATACATTGAAGCAGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgc                      <  1:96844/150‑1 (MQ=25)
                                                                                                                        aGTGATAATCCGTCACCCCGATACGGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgcc                     <  1:1817669/150‑1 (MQ=255)
                                                                                                                          tGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAcgccgc                   <  1:1090273/150‑1 (MQ=255)
                                                                                                                           gATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCa                  >  1:772263/1‑150 (MQ=255)
                                                                                                                             ttATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATc                <  2:417989/150‑1 (MQ=255)
                                                                                                                             ttATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATc                <  2:787118/150‑1 (MQ=255)
                                                                                                                             ttATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACATGCAATATATTGAATCTGCAGGCCTTTGTAGGCCGGATAAGGCGTTAACGCCGCATc                >  2:81890/1‑150 (MQ=255)
                                                                                                                               aTCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAg              <  1:1398605/150‑1 (MQ=255)
                                                                                                                                tCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCGGCAATATATTGAATCTGCAGGCCTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAgg             >  2:397367/1‑150 (MQ=255)
                                                                                                                                gccGTCACCCCGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACATTGTAGGCCGGATAAGGCGTTAACGCCGCATCAgg             >  2:1954978/2‑150 (MQ=255)
                                                                                                                                 ccGTCACCCCGCCAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGc            >  1:1629643/1‑150 (MQ=255)
                                                                                                                                  cGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCa           >  1:1562131/1‑150 (MQ=255)
                                                                                                                                   gTCACCCCGAAACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGAAGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCAt          <  1:184153/150‑1 (MQ=255)
                                                                                                                                    tCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCAtt         >  1:1416470/1‑150 (MQ=255)
                                                                                                                                     cACCCCGACAGTATCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATAAATTGAATCTGCAGGACTTTGTTGGCCGGATAAGGCGTTAACGCCACATCAGGCATTa        >  2:1030912/1‑150 (MQ=25)
                                                                                                                                       ccccGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAAc      >  1:1200747/1‑150 (MQ=255)
                                                                                                                                       ccccGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAAc      >  2:2060566/1‑150 (MQ=255)
                                                                                                                                        cccGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACa     <  1:1115969/150‑1 (MQ=255)
                                                                                                                                         ccGATATTGTGGGGGTTAGGGCGGCTGATGACAAATGCAAAATAGCCTGATGCTCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACaa    <  2:1513703/150‑1 (MQ=21)
                                                                                                                                             |                                                                                                                                                   
TTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAACGCCGCATCAGGCATTAACAAAG  >  NC_000913/857435‑857723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: