Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 909,610 G→T 7.0% T480T (ACC→ACA poxB ← pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913909,6100GT 7.0% 292.0 / 4.3 112T480T (ACC→ACApoxBpyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding
Reads supporting (aligned to +/- strand):  ref base G (57/45);  new base T (8/2);  total (65/47)
Fisher's exact test for biased strand distribution p-value = 1.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

CTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAACCGCCATCGCCGCA  >  NC_000913/909462‑909759
                                                                                                                                                    |                                                                                                                                                     
cttTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCgg                                                                                                                                                      <  1:1839783/150‑1 (MQ=255)
     gCGACCACCACCTCCACCAACCCCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACAGGGCTACCCGTTATGCCGCACGCTTCGGCAATGCCGGCAAAGATTGTGTCGTGTTGTTCGGTGCCg                                                                                                                                                 >  2:1528626/1‑150 (MQ=255)
      cGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCgt                                                                                                                                                >  1:1677347/1‑150 (MQ=255)
       gACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCgtc                                                                                                                                               >  1:2066941/1‑150 (MQ=255)
       gACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCgtc                                                                                                                                               <  2:277891/150‑1 (MQ=255)
          caccacATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCTGTGCCgtcagt                                                                                                                                            <  1:1984537/150‑1 (MQ=255)
          caccacATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCgtcagt                                                                                                                                            <  1:1768499/150‑1 (MQ=255)
           accacATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCgtcagtc                                                                                                                                           >  1:1165250/1‑150 (MQ=255)
           accacAACCACCAACACCGTACCGTCGAAGGAGAAACCCAGTACCACGGCTACATCAAATACCGACGCATATACTACACGGATACCCGTAATGCCACACGCTTCGGCAATGCGCGCAAACTAAGTGTCCTGTAGTTCGGTGACgtcactc                                                                                                                                           >  2:1515467/1‑150 (MQ=255)
            ccacATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGCCGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCgtcagtca                                                                                                                                          >  1:609095/1‑150 (MQ=255)
               caTCCACCAACACCGGCCCGTCGATGGAGAAGGCGCGTGGCAGGGCTTCATCAACTTCCGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTGCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAAt                                                                                                                                       <  1:1909744/150‑1 (MQ=255)
               caTCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCaaaa                                                                                                                                       >  1:891074/1‑149 (MQ=255)
               caTCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAAt                                                                                                                                       >  2:632613/1‑150 (MQ=255)
               caTCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAAt                                                                                                                                       <  2:1936027/150‑1 (MQ=255)
               caTCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACACGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCTGTGGCGTCAGTCAAAt                                                                                                                                       >  2:1492538/1‑150 (MQ=255)
                 tCCACCACCACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTATACACGGATACCCGTAATGCCGCACTCTTCGGCAATGCTGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAAtcg                                                                                                                                     >  2:1136658/1‑148 (MQ=255)
                   cagcaACACCGGACCGTCGATGGAGACGGCGGGTTGCAGGGGTTCATCAACTTCAGACGGTTTTTCTACACTGATACCCGTAATGGCGAACGATTCGGTAATGCGGGCAATGTTTGTGTCGTGTCGTTCGGTGCCGTCAGTCAAATAGcc                                                                                                                                   <  1:32877/147‑1 (MQ=255)
                   caccaACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGcc                                                                                                                                   <  1:1158890/150‑1 (MQ=255)
                    accaACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGcca                                                                                                                                  >  1:1890312/1‑150 (MQ=255)
                                ccGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCAtc                                                                                                                      >  1:1822151/1‑150 (MQ=255)
                                  gTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCAtctc                                                                                                                    >  1:5049/1‑150 (MQ=255)
                                         gagaAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACACGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGcc                                                                                                             >  2:414467/1‑150 (MQ=255)
                                          agaAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATTCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGACAACAGCATTCATCTCCATCGccc                                                                                                            >  2:332971/1‑149 (MQ=255)
                                          agaAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCca                                                                                                            <  2:152372/150‑1 (MQ=255)
                                           gaAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCcac                                                                                                           <  2:1733532/150‑1 (MQ=255)
                                                cgcgTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGc                                                                                                      >  2:169624/1‑150 (MQ=255)
                                                  cgTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGccc                                                                                                    <  2:2090558/150‑1 (MQ=255)
                                                     tGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGc                                                                                                 <  1:2055801/150‑1 (MQ=255)
                                                     tGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGc                                                                                                 <  1:1222239/150‑1 (MQ=255)
                                                      gCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCa                                                                                                >  1:415986/1‑150 (MQ=255)
                                                      gCAGGGCTTCAACAACTTCAGACGCTTTTTCTACACGGCTACCCGTAATGCCGCACGCTTCGGCCATGCGCGCAAAGTATGTGTCGTGTAGTTCGGGGCCGTCAGTCAAAAAGCCCCCAGCATTCATCTCCATCGCCACAAAGCCCAGcc                                                                                                >  2:213263/1‑149 (MQ=255)
                                                       cAGGGCGTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTGCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCAc                                                                                               <  1:358838/150‑1 (MQ=255)
                                                         gggCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGGCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGc                                                                                             <  1:1086291/150‑1 (MQ=255)
                                                           gCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCtg                                                                                           >  1:835436/1‑150 (MQ=255)
                                                              tcatcaACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCtgttg                                                                                        >  1:100758/1‑150 (MQ=255)
                                                               catcaACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTAAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCtgttgg                                                                                       >  2:1847178/1‑149 (MQ=255)
                                                                atcaACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTgttgtt                                                                                      <  2:869904/150‑1 (MQ=255)
                                                                  caACTTCAGCCGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTaa                                                                                    >  1:445631/1‑150 (MQ=255)
                                                                    aCTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAg                                                                                  <  2:1761567/150‑1 (MQ=255)
                                                                      ttCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCGCAAAGCCCAGCACGCTGTTGTTAAAgac                                                                                <  1:755107/150‑1 (MQ=255)
                                                                      ttCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTGCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAgac                                                                                <  1:1067413/150‑1 (MQ=255)
                                                                        cAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGGGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGGTGGTAAAgccga                                                                              >  2:539878/1‑150 (MQ=255)
                                                                          gACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACa                                                                            >  2:1132211/1‑150 (MQ=255)
                                                                            cGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAAt                                                                          >  1:1091533/1‑150 (MQ=255)
                                                                            cGCTTTTTCTACACGGATACCCGTAATGACGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAAt                                                                          >  1:543701/1‑150 (MQ=255)
                                                                              cTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGGCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAAttt                                                                        <  2:1137750/150‑1 (MQ=255)
                                                                              cTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGATCTCTGACGTCACACAAATATAAAAAAACATTCATCTCCATCGCCACAAAGCCCAGCAGGCTGTTGTTAAAGACGACAAttt                                                                        >  2:1898129/1‑150 (MQ=255)
                                                                              cTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGGCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAAttt                                                                        <  1:203903/150‑1 (MQ=255)
                                                                               tttttCTACACGGATACCCGTAAAGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCGATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAAtttt                                                                       >  1:500030/1‑150 (MQ=255)
                                                                                 tttCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCa                                                                     >  1:887615/1‑150 (MQ=255)
                                                                                   tCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACt                                                                   <  2:449378/150‑1 (MQ=255)
                                                                                   tCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGATCTCTGACGTCACACAAAAATAAAAAAAATTACATCTCCATCGCCCCAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTATCACt                                                                   >  2:1567403/1‑150 (MQ=255)
                                                                                   tCTACACGGATACCCGTAAAGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATACCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTGAAAGACGACAATTTTCAca                                                                   >  1:537803/1‑149 (MQ=255)
                                                                                     tACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTgg                                                                 >  1:858799/1‑150 (MQ=255)
                                                                                      acacGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGc                                                                >  1:1337513/1‑150 (MQ=255)
                                                                                       cacGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCa                                                               <  2:1674495/150‑1 (MQ=255)
                                                                                        acGGATACCCGTAATGCAGCACGCTTCGGCAATGCGGGAAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGATTTCATCTCCATCGCCACAAAGCCCAGCAGGCTGTTGTTAAAGCCGACAATTTTCAATGGCAg                                                              >  1:960671/1‑150 (MQ=255)
                                                                                         cGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGACGTCAGTCAAATAGCAACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGt                                                             >  2:1458129/1‑150 (MQ=255)
                                                                                            aTACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGGCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTc                                                          <  2:1260375/150‑1 (MQ=255)
                                                                                               cccGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGCCGACAATTTTCACTGGCAGTTTCATc                                                       >  1:801659/1‑150 (MQ=255)
                                                                                               cccGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACAAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGGTTCATc                                                       >  1:924460/1‑150 (MQ=255)
                                                                                               cccGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGAGTCGTGTAGTTCTGTGCCGTCATTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATc                                                       <  2:1266929/150‑1 (MQ=255)
                                                                                                      tGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCacta                                                >  1:674591/1‑150 (MQ=255)
                                                                                                       gCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCactac                                               >  1:1296655/1‑150 (MQ=255)
                                                                                                       gCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCactac                                               >  1:53621/1‑150 (MQ=255)
                                                                                                        ccGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCTGTGACGTCACACAAAAATACACCATCTATCATCTACATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCactact                                              >  2:1834043/1‑150 (MQ=255)
                                                                                                          gCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGGGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTga                                            <  2:2095254/150‑1 (MQ=255)
                                                                                                          gCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGATCTCTGACGTCACACAAAAATAAAAAAAAAATAATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTga                                            >  2:655236/1‑150 (MQ=255)
                                                                                                           cACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTgag                                           >  1:1495231/1‑150 (MQ=255)
                                                                                                              gCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAgg                                        <  1:2029462/150‑1 (MQ=255)
                                                                                                                    gCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCg                                  <  1:1096587/150‑1 (MQ=255)
                                                                                                                    gCAATGCGGGAAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATATCCACCAGCTTTCATCTCCATCGCCACAAATCCCCGCACGCAGTTTTTAAAGACGACAATTTTCACTGGCAGTTTAATCAGCACTACTGAGATGAAATCg                                  >  1:1436392/1‑150 (MQ=255)
                                                                                                                      aaTGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGcc                                <  2:2066941/150‑1 (MQ=255)
                                                                                                                      aaTGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGcc                                <  2:1822151/150‑1 (MQ=255)
                                                                                                                       aTGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGccc                               >  1:959382/1‑150 (MQ=255)
                                                                                                                         gCGGGCATAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCAt                             <  2:1703334/150‑1 (MQ=255)
                                                                                                                           gggCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCa                           <  1:427116/150‑1 (MQ=255)
                                                                                                                           gggCAAAGTTTGTGTCGTGTAGATCTCTGACGTCACACAAAAATAAAAAAAAATAAATATCCATAGCCACAAAGCCCCGCACGGTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCAGTAGTGAGAGGCATTCGCCCATCa                           >  2:39817/1‑150 (MQ=255)
                                                                                                                            ggCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCaa                          <  2:448692/150‑1 (MQ=255)
                                                                                                                            ggCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCaa                          <  2:1165250/150‑1 (MQ=255)
                                                                                                                            ggCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCaa                          >  1:642023/1‑150 (MQ=255)
                                                                                                                              cAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACa                        >  1:384302/1‑150 (MQ=255)
                                                                                                                              cAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACa                        <  2:1677347/150‑1 (MQ=255)
                                                                                                                                aaGTTTGTGTCGGGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATg                      <  1:169624/150‑1 (MQ=255)
                                                                                                                                  gTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCt                    >  1:909088/1‑150 (MQ=255)
                                                                                                                                  gTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTATCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCt                    <  2:379713/150‑1 (MQ=255)
                                                                                                                                        gtCGTGTAGTTCTCTGACGTCAGTCAAATAGCCACCAGATTTCATCTCCATCGCCACAAAGCCCAGCCCGCTGTTGTTAAAGCCGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCCACATGCTAAAAcc              >  2:102538/1‑150 (MQ=255)
                                                                                                                                        gtCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGGTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAAcc              <  1:2062224/150‑1 (MQ=255)
                                                                                                                                         tCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAACCg             >  1:2066736/1‑150 (MQ=255)
                                                                                                                                         tCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAACCg             <  2:931483/150‑1 (MQ=255)
                                                                                                                                          cGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAACCGc            <  2:5049/150‑1 (MQ=255)
                                                                                                                                            tgtAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAACCGCCa          >  2:1905848/1‑150 (MQ=255)
                                                                                                                                              tAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAACCGCCATc        <  1:510728/150‑1 (MQ=255)
                                                                                                                                                gTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAACCGCCATcgc      >  2:1950291/1‑150 (MQ=255)
                                                                                                                                                  tCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAACCGCCATcgccg    <  2:609095/150‑1 (MQ=255)
                                                                                                                                                  tCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAACCGCCATcgccg    <  1:2039419/150‑1 (MQ=255)
                                                                                                                                                   cGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAACCGCCATcgccgc   >  2:443424/1‑150 (MQ=255)
                                                                                                                                                   cGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAACCGCCATcgccgc   <  1:932339/150‑1 (MQ=255)
                                                                                                                                                    ggTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCAGTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAACCCGCCAGcgccgcc  >  2:330909/1‑149 (MQ=255)
                                                                                                                                                    ggTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTGTCAGCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTTAAACCGCCATCGCCGca  >  1:391126/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTTTGGCGACCACCACATCCACCAACACCGGACCGTCGATGGAGAAGGCGCGTTGCAGGGCTTCATCAACTTCAGACGCTTTTTCTACACGGATACCCGTAATGCCGCACGCTTCGGCAATGCGGGCAAAGTTTGTGTCGTGTAGTTCGGTGCCGTCAGTCAAATAGCCACCAGCTTTCATCTCCATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAACCGCCATCGCCGCA  >  NC_000913/909462‑909759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: