BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,287,420	G→C	5.3%	intergenic (‑92/+118)	tyrV ← / ← tyrT	tRNA‑Tyr/tRNA‑Tyr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,287,420	0	G	C	5.3%	309.4 / 6.0	106	intergenic (‑92/+118)	tyrV/tyrT	tRNA‑Tyr/tRNA‑Tyr
Reads supporting (aligned to +/- strand): ref base G (48/51); new base C (4/2); total (52/54)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

Only 100 of 109 total aligned reads displayed.

GTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAG  >  NC_000913/1287275‑1287569

gTCGCTGTCGGCAGCTTTCCGGTCTGCGCCCTTTGGCCGCTCGGGAACCCCCCCAGGGGTAATTCAAATGTTGAGGAATTGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGc                                                                                                                                                   <  1:818279/150‑1 (MQ=38)

    aTGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACg                                                                                                                                               <  2:2085162/150‑1 (MQ=255)

       ccGGCCGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCCCCAGGGGTATTTCAAATTTTGAGGTACTGCTAGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTtc                                                                                                                                            <  1:578079/149‑1 (MQ=39)

       ccGGCAGATTTACAGCCTGCGCCCTTTGGCCGCCCGGGAACCCCACCAGGGGAAATTCAAATCTGGCGGTAATGCTGGAGATGGTGGGGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCACAGGCAACggcc                                                                                                                                            <  1:2039918/149‑4 (MQ=255)

       ccGGCAGATATACAGTCTGCGCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGACGTAATGCTTGAGATGGTGGGGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCACAGGCAACggcc                                                                                                                                            <  1:1941697/149‑4 (MQ=38)

          gCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCATCGGGCCGTTGCCAGTGGCAACGTtctca                                                                                                                                         >  1:1370149/1‑149 (MQ=255)

          gCAGATTTACAGTCTGCTCACTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCAGTTGCCGGTGGCAACGTtctct                                                                                                                                         >  1:1322223/1‑150 (MQ=255)

             gATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCATCACCCTTCGGTCCGTTGCCTGTGGCAACGTTCTCTCGc                                                                                                                                      >  2:601313/1‑150 (MQ=255)

             caTTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGCGGAACTGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGc                                                                                                                                      <  1:820066/149‑1 (MQ=255)

               tttACAGTCTGCTCCCTTTGGCCGCTCGTGAACCCCAGCAGGTGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCtt                                                                                                                                    <  1:1074295/150‑1 (MQ=255)

                ttACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCttt                                                                                                                                   >  1:1984711/1‑150 (MQ=255)

                 tACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTc                                                                                                                                  >  1:350691/1‑150 (MQ=255)

                 tACAGGCTGCTCCCATTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGCGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTc                                                                                                                                  <  2:1162380/150‑1 (MQ=255)

                  aCAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCg                                                                                                                                 >  2:1232125/1‑150 (MQ=255)

                  aCAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCg                                                                                                                                 >  2:2050806/1‑150 (MQ=255)

                     gTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGATTGAGATGGTGGTGGGGGAAGGATTATTAGTCGCTTCGCTCCTCACACTTCGGGCCGTTTCCTCTGGCAACGTTCTCTCGCtttagata                                                                                                                              >  1:1167323/1‑145 (MQ=39)

                       cTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCtcga                                                                                                                            >  1:1272039/1‑150 (MQ=255)

                       cTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCtcga                                                                                                                            <  1:652784/150‑1 (MQ=255)

                            cccTTTGGCCGCTCGGGAACCCCACCAGTGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTAGTCGATTCGCTCCTCACCCTTCGGGCCGTTGCCGGTGTCATCGTTCTCTCGCTTTCTCtcgaatcga                                                                                                                       >  2:331007/1‑150 (MQ=255)

                               tttGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGCTGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAAcc                                                                                                                    <  1:968406/150‑1 (MQ=255)

                                   gCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAg                                                                                                                <  1:514204/150‑1 (MQ=255)

                                    ccGCTCGGGAACCCCACCAGGGGTAATTCAATTTTTGAGGTAATGCATGAGATGGTGGTGGGGGAAGGATTATTAGTCGCTTCGCTCCTCACGCTTCGGTACGTTTCCTGTGTCTTCGTTCTCTCGCTTTATATCGACTCGAACCttttt                                                                                                               >  2:527785/1‑147 (MQ=255)

                                    ccGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGt                                                                                                               >  2:1462005/1‑150 (MQ=255)

                                    ccGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGt                                                                                                               >  2:1567671/1‑150 (MQ=255)

                                    ccGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGACGTTGCCTGTGGCAACGTTCTCTCGCTTTAGCTCGAATCGAACCTTAGt                                                                                                               >  1:1233771/1‑150 (MQ=255)

                                     cGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTc                                                                                                              >  2:642781/1‑150 (MQ=255)

                                         cGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAg                                                                                                          >  1:1157211/1‑150 (MQ=255)

                                         cGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAg                                                                                                          >  1:1104356/1‑150 (MQ=255)

                                           gtaaCCCCAGCAGGGGTAATTCAATTTTTGCGGTAATGCTTGTGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGACAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGt                                                                                                        <  2:1563477/148‑1 (MQ=37)

                                             caCCGCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTtc                                                                                                      <  1:523407/149‑1 (MQ=255)

                                               ccccACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTtctc                                                                                                    <  2:1272039/150‑1 (MQ=255)

                                                 ccaccaGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCAc                                                                                                  <  2:1758522/150‑1 (MQ=255)

                                                   cccaGGGGTAATTCAATTTTTGCGGTAATGATTGAGATGGGGGGGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTCGACTAAGGTtcgattcg                                                                                                <  2:532467/149‑7 (MQ=255)

                                                    ccaGGGGTAATTCATATTTTGCGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTCGACTAAGGTtcgattcga                                                                                               <  1:1654729/150‑8 (MQ=37)

                                                     caGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCtt                                                                                              >  1:1696110/1‑150 (MQ=255)

                                                      agtggTTATTAAAATTTTGAGGTAATTCTGGAGATGGGTGTGGGGGACGTATTATGCGTCGGTTCGCTCCTCACGCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTc                                                                                             <  1:828806/147‑1 (MQ=255)

                                                               tCAAATTTAGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGTGACGTTGCCTGTGGCTACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAg                                                                                    >  1:331333/1‑150 (MQ=255)

                                                                cAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTACCCGATGAGt                                                                                   >  1:1375853/1‑150 (MQ=255)

                                                                cAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCATAGTCGAATGTTCTCACCCTTCCACGATGAGt                                                                                   >  1:675438/1‑150 (MQ=255)

                                                                          ggTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTc                                                                         >  1:1859983/1‑150 (MQ=255)

                                                                            ttttgattGTGATGGTGGTGGGGGAAGGATTATTCTTCGCTTCGCTCCTCTCCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTcac                                                                       <  2:719208/144‑1 (MQ=25)

                                                                            tattGCTTGAGATGGTGGTGGGGGAAGGATTATGCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTTGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTcac                                                                       <  2:372411/147‑1 (MQ=255)

                                                                            tAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTcac                                                                       <  1:1996715/150‑1 (MQ=255)

                                                                             aaTGCATGAGATGGTGGTGGGGGAAGGATTATTCGTAGCTTCGCACCTCACCCTTCGGGCCGTTGCCTCTGGCAACGTTCTCTCGCTTTAGCTCGAATCCAACCTTAGTAGAAGGTTCTAACCATACACAGATGAGTGAAAACTTTcaca                                                                      >  2:697219/1‑150 (MQ=25)

                                                                              aTGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACaa                                                                     <  1:2008276/150‑1 (MQ=255)

                                                                               tGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCATCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACaaa                                                                    >  1:502164/1‑149 (MQ=255)

                                                                                gCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAAtc                                                                   >  2:973826/1‑150 (MQ=255)

                                                                                 cTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAAtct                                                                  >  2:159306/1‑150 (MQ=255)

                                                                                  tagtgaTGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAAtctc                                                                 <  2:1422859/146‑1 (MQ=255)

                                                                                    gagaTGGTGGTGGGGGAAGGATTATTCGTCGCTTCGATCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCAc                                                               >  2:598439/1‑150 (MQ=255)

                                                                                     cgaTGGAGGTGCGTGTAGCTTTATAGGTCGCTTGTCTCCTCACGCGTGGGCACGTTTTCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCAcc                                                              <  1:1718318/149‑1 (MQ=255)

                                                                                        tggtggtggGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAACGTTATCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGaa                                                           >  2:791376/1‑150 (MQ=255)

                                                                                         ggtggtggGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGaat                                                          >  2:1743051/1‑149 (MQ=255)

                                                                                         ggtggtggGGGAAGGATTACTCATCGCTTCGCGCCTCTCCCTTCGGGTCGTTGCCTGTGGCAACGTTCTCTCGCTTGCGCTCTAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCTTACTTTCACAATCTCACCGatg                                                          <  1:271013/150‑3 (MQ=18)

                                                                                            ggtggGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTa                                                       <  2:1984711/150‑1 (MQ=255)

                                                                                            ggggggggAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTa                                                       <  1:1787778/147‑1 (MQ=255)

                                                                                              tggGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAATCTTTCACAATCTCACCGAAGTTAcc                                                     >  2:64593/1‑150 (MQ=255)

                                                                                               gggggAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACca                                                    <  1:809635/150‑1 (MQ=255)

                                                                                                ggggAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACcac                                                   <  1:849933/150‑1 (MQ=255)

                                                                                                 gggAAGGATTATTCGTCGCTTCGCGCCTCCCCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACcaca                                                  <  1:1611409/150‑1 (MQ=255)

                                                                                                     atgATTATTCGTCGATTAGCTCCTCACCCTTCTGGCCGTTTCCGGTGGCAACGTTCTCTCGCTTTCGCTCTAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGc                                              <  1:1774984/148‑1 (MQ=255)

                                                                                                      ggATTATTCGTCGCTTCGCTCCTCACTCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACGCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCt                                             <  2:232876/150‑1 (MQ=255)

                                                                                                      ggATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCt                                             >  2:548242/1‑150 (MQ=255)

                                                                                                        attattCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCtgt                                           <  2:1907237/150‑1 (MQ=255)

                                                                                                          tattCGTCGCTTGGCTCCTCACACTTCGGGCCGTTGCCTGTGGCAACGTTCTCTAGCTTTCGCTAGAATCGAACCTTAGTCTAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTgtgg                                         <  1:1878730/150‑1 (MQ=255)

                                                                                                          tattCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCtgttg                                         >  2:145982/1‑148 (MQ=255)

                                                                                                          tattCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTgtgg                                         <  1:321517/150‑1 (MQ=255)

                                                                                                           attCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTgtggt                                        >  1:1431829/1‑150 (MQ=255)

                                                                                                           attCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTgtggt                                        <  2:350691/150‑1 (MQ=255)

                                                                                                           attCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTgtggt                                        <  1:621769/150‑1 (MQ=255)

                                                                                                           attCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTgtggt                                        >  2:88072/1‑150 (MQ=255)

                                                                                                              cgtcgCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGaa                                     >  2:985944/1‑150 (MQ=255)

                                                                                                               gtcgCTTCGCTCCTCACCCTTCGGGCCGTTGCGTGTGGCAACGTTCTCTCGCTTTCGCTCGTATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCAGATTCTCACCGAAGTTACCACATCGCTGTGGTGAAt                                    <  1:1812939/150‑1 (MQ=255)

                                                                                                                 cgcttcgctCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTa                                  >  2:12562/1‑150 (MQ=255)

                                                                                                                 cgcttcgctCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTa                                  >  1:286364/1‑150 (MQ=255)

                                                                                                                 cgcttcgctCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTa                                  >  1:762878/1‑150 (MQ=255)

                                                                                                                     tcgctCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTAtggt                              <  1:642781/150‑1 (MQ=255)

                                                                                                                      cgctCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCAAAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTAtggtg                             >  2:1863585/1‑150 (MQ=255)

                                                                                                                       tctcctcCCCGATCGGGCCGCTGCCTGCGGCAACGTTCTCTCGCTTGCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTAtggtgg                            <  2:871411/149‑1 (MQ=255)

                                                                                                                       gctCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTAtggtgg                            >  1:1827533/1‑150 (MQ=255)

                                                                                                                       gctCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTAtggtgg                            <  1:1876892/150‑1 (MQ=255)

                                                                                                                         tcctcACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCACACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTAtggtggtg                          >  2:520181/1‑150 (MQ=255)

                                                                                                                           ctcACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGCACCTTAGTCGAAGGTTCTCACCCTGCCCCGCTGAGTGCAGACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAAGTTTGGTGGTggg                        >  1:1496460/1‑150 (MQ=255)

                                                                                                                           ctcACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTggg                        <  1:1092636/150‑1 (MQ=255)

                                                                                                                            tcACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTgggg                       <  1:805791/150‑1 (MQ=255)

                                                                                                                            tcACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTgggg                       <  2:1152092/150‑1 (MQ=255)

                                                                                                                             cACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCATCCCCGATGAGTGCAAACTTTAACAATCTCAACGAAGTTACCACATCTCTGTGGTGAATTATTGTGGTggggg                      >  2:1902685/1‑150 (MQ=255)

                                                                                                                                ccTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCAAATCGCTGTGGTGAATTATGGTGGTGGGGGAAg                   >  1:1918881/1‑150 (MQ=255)

                                                                                                                                  gagagaacGTTGCCACAGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGa                 >  2:1494385/8‑150 (MQ=255)

                                                                                                                                   agagaacGTTGCCACAGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGAt                >  2:515422/7‑150 (MQ=255)

                                                                                                                                      ggCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGATTTATGGTGGTGGGGGAAGGATTCg             <  2:1198677/150‑1 (MQ=255)

                                                                                                                                      ggCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCg             <  1:601313/150‑1 (MQ=255)

                                                                                                                                            tGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCtt       >  1:865510/1‑150 (MQ=255)

                                                                                                                                             gCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTc      >  1:909694/1‑150 (MQ=255)

                                                                                                                                              ccTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCg     >  2:30172/1‑150 (MQ=255)

                                                                                                                                               cTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGa    <  2:1583186/150‑1 (MQ=255)

                                                                                                                                               cTGTGGCAACGAGCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTGTCACCCTTCCCCGAGGAGTGCAAACTTTCACAATCTCACCGAATTAACCACATCGCTGTGGTGAAATATGGTGGTGGGGGAAGGATTCGAACCTTCGa    <  2:1370149/150‑1 (MQ=255)

                                                                                                                                               atgtgGCAACGTTCTCTCGCTTTCGCTAGAATCGAACCTTAGTCGCAGGTTCTCACCCTTCCCCGATGAGTGCAACCTTTCCCAATCTCCCCGAAGTTACCACATAGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGa    <  2:1233771/149‑1 (MQ=255)

                                                                                                                                                tgtgGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGaa   >  1:40793/1‑150 (MQ=255)

                                                                                                                                                 gtgGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAg  <  2:1104356/150‑1 (MQ=255)

GTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAG  >  NC_000913/1287275‑1287569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`