Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,314,682 A→T 7.7% intergenic (+24/+36) ompW → / ← yciE outer membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,314,6820AT 7.7% 336.6 / 12.8 123intergenic (+24/+36)ompW/yciEouter membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (71/42);  new base T (7/3);  total (78/45)
Fisher's exact test for biased strand distribution p-value = 7.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 128 total aligned reads displayed.

AACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGC  >  NC_000913/1314533‑1314831
                                                                                                                                                     |                                                                                                                                                     
aaCATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTaa                                                                                                                                                       <  2:1232399/150‑1 (MQ=255)
  cATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAatt                                                                                                                                                     >  1:1034119/1‑149 (MQ=255)
   aTGTCAGTGGGGTCCATGGATATCGATACCACCGCCAATTATAAGCGGGCCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAatat                                                                                                                                                    <  2:2038058/150‑1 (MQ=255)
     gTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATAc                                                                                                                                                  >  1:1294918/1‑150 (MQ=255)
       cAGTGTGGTACATGGATATCGCTACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGCTGTTTATGTTCTCAGCAGGATATCGATTTTAATTCCGCAAAAAAACGACCCCGTAAAAAACgg                                                                                                                                                >  1:107399/1‑150 (MQ=255)
       cAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACgg                                                                                                                                                >  2:601558/1‑150 (MQ=255)
        aGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTATTATACggg                                                                                                                                               >  2:798209/1‑150 (MQ=255)
        aGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACggg                                                                                                                                               >  2:1249173/1‑150 (MQ=255)
         ttgtgGTACATGGAAATCGATACCACCGCCAATTATAAGCTGGTCGTTGCACAGCAACAAGATAGCGTACGCCTCGATCCGGGGGTGTTATTGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACgggg                                                                                                                                              <  1:1590934/149‑1 (MQ=255)
         gtgtGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACgggg                                                                                                                                              <  1:1095054/150‑1 (MQ=255)
          tgtgGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGt                                                                                                                                             <  1:1202048/150‑1 (MQ=255)
           gtgGTACATGGATATCGATACCACCGCCATTTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGTTGTTTATGTTCTCAGCAGGATATCGTTTTTTATTCCGCACAAAAACGACCCAGTAATATACGGGGta                                                                                                                                            >  1:811631/1‑149 (MQ=255)
             ggTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCaa                                                                                                                                          >  2:1938327/1‑150 (MQ=255)
             ggTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGAACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCAGCACAAAAACGACCCCCTAATATACGGGGTCaa                                                                                                                                          >  1:983925/1‑150 (MQ=255)
             ggTACATGGATATCGAAACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTACGCACAAAAACGACCCAGTAATATACGGGGTCaa                                                                                                                                          >  1:1837233/1‑150 (MQ=255)
              ctACATGGATATCGACACCACCGCCAATTAAAAGCTGGGCGGTGAACAGCAACACCAAAGCGTACGGCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTATATTACGGGGTcgtt                                                                                                                                         <  2:387400/149‑4 (MQ=255)
                aCATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATaa                                                                                                                                       >  1:2102160/1‑150 (MQ=255)
                 cATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGTCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAg                                                                                                                                      >  2:1685372/1‑150 (MQ=255)
                  aTGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTATATTACGGGGTcgtttttg                                                                                                                                     <  1:1229980/150‑8 (MQ=255)
                     gATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACa                                                                                                                                  >  1:68415/1‑150 (MQ=255)
                       aatCGATACCACCGCAAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCAGTGGGTGGCTATGTTCTCAGCAGGATATCGTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATgg                                                                                                                               <  2:76489/149‑1 (MQ=255)
                        atCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATgg                                                                                                                               <  1:695854/150‑1 (MQ=255)
                         tCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGt                                                                                                                              >  2:843429/1‑150 (MQ=255)
                           gATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGAGATCGTTTTTAAATCCGCACAAAAACGACCCCGTAATATACTGGGTCTATAATGACATGGtat                                                                                                                            >  1:2056363/1‑150 (MQ=255)
                            aTACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGtata                                                                                                                           >  2:393616/1‑150 (MQ=255)
                               ccaccGCCAATTATAAGATGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTAAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAg                                                                                                                        >  2:315541/1‑150 (MQ=255)
                                caccGCCAATTATAAGCTGGGTGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAgg                                                                                                                       >  1:1740338/1‑150 (MQ=255)
                                caccGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAgg                                                                                                                       >  2:1141629/1‑150 (MQ=255)
                                 accGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTCCGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAggg                                                                                                                      <  2:96871/150‑1 (MQ=255)
                                  ccGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGt                                                                                                                     >  1:511457/1‑150 (MQ=255)
                                  ccGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTAAATTCCTCACAAAAACGACCCCGTAATATACTCGGTCAATAAGGACATGGTATAAAGGGt                                                                                                                     >  1:1154155/1‑150 (MQ=255)
                                     ccAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCATCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAATGACATGGTATAAAGGGtttt                                                                                                                  >  2:296066/1‑150 (MQ=255)
                                        aTTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTattatt                                                                                                               >  2:616813/1‑150 (MQ=255)
                                             aaGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATttcttg                                                                                                          >  2:395219/1‑149 (MQ=255)
                                                 tGGGCGGTGCACAGAAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACATAAACGACCCCGTAATATACGTGGTGAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCtt                                                                                                      <  1:47369/150‑1 (MQ=255)
                                                  gggCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGACAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTc                                                                                                     >  2:2079594/1‑150 (MQ=255)
                                                     cGGTGCACAGCAACACGATAGCGTACGCCTCGCTCCGTGGGTGTTTATGTTCGCAGCAGGATATCGTTTTTAATTCCGCACAAAAAAGACCCCGTAATATACGGGGGCAATAAGGACATGGTAAAAAGGGGATTATTTCTTCGATTCTAc                                                                                                  >  1:833030/1‑150 (MQ=255)
                                                          cacaGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCAt                                                                                             <  1:511586/150‑1 (MQ=255)
                                                          cacaGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCAt                                                                                             >  2:2102424/1‑150 (MQ=255)
                                                          cacaGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGCATATCGTTTAGAATTCAGCACAAAAAAGACCCCGTAAAATACGGGCTCTAAAAGGACATGGTATAAAGGGGATTATTTCATCGCTTCTACGCCAt                                                                                             >  2:1986407/1‑150 (MQ=255)
                                                           acaGCAACACGAAAGCGTACGCCTCGATCCGTGGGTGGTTATGTGCTCAGCAGGATATCGTTTTTATTTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTCCGCTTCTACGCCATc                                                                                            <  1:125167/150‑1 (MQ=255)
                                                            caGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATTCGGGGTCAATAATGACATGGTTTAAAGGGTATTATTTCTTCGCTTCTACGCCATCa                                                                                           >  2:141487/1‑150 (MQ=255)
                                                            caGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATcg                                                                                           >  2:236282/1‑149 (MQ=255)
                                                            caGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAAGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCa                                                                                           >  2:338192/1‑150 (MQ=255)
                                                               cAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGACAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCATCTACGCCATAAGtt                                                                                        >  1:1180372/1‑150 (MQ=255)
                                                                            tACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTa                                                                           >  2:1155544/1‑150 (MQ=255)
                                                                            tACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTa                                                                           >  2:1503323/1‑150 (MQ=255)
                                                                               gCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATTTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaaa                                                                        >  1:1840968/1‑150 (MQ=255)
                                                                               gCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGGAATATACGGGGTCAATAAGGACATGGTATAAAGGGTAGTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaaa                                                                        >  2:1960436/1‑150 (MQ=255)
                                                                               gCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTACGCACAAAAACGACCCCGTAATATACGGGGTCAAAAAGGACATGGTATAAAGGGTATTTTTTCTTCGCTTCTACGCCCTCAGTTTCAGAGCGAATTaaaa                                                                        >  2:339469/1‑150 (MQ=255)
                                                                                ccTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaaaa                                                                       >  1:2101880/1‑150 (MQ=255)
                                                                                 cTCGATCCGTGGGTGCTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAAt                                                                      <  2:1215160/150‑1 (MQ=255)
                                                                                    gATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtc                                                                   <  1:328547/150‑1 (MQ=255)
                                                                                    gATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtc                                                                   <  2:68415/150‑1 (MQ=255)
                                                                                              tgtTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGAGAATTAAAAATTTCACAGTTGttt                                                         >  2:2065983/1‑150 (MQ=255)
                                                                                                tttATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGc                                                       <  1:260752/150‑1 (MQ=255)
                                                                                                 ttATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCg                                                      >  1:1861124/1‑150 (MQ=255)
                                                                                                 ttATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCg                                                      >  2:1608470/1‑150 (MQ=255)
                                                                                                 ttATGTTCTCAGAAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGGATCAGAGCGAATTAAAAATTTCTCACTTGTTTGCg                                                      <  2:1885667/150‑1 (MQ=255)
                                                                                                 ttAAGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCg                                                      <  2:1529190/150‑1 (MQ=255)
                                                                                                  tttgttgtCATCAGTATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGTGTCAATTAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTTAAAATTTCTCAGTTGTTTGCgg                                                     <  2:983925/148‑1 (MQ=255)
                                                                                                    tGTTCTCAGCAGGATATCGTTTTTAATTACGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGAGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGATTTTTGCGGaa                                                   >  1:1308799/1‑150 (MQ=255)
                                                                                                     gTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGATCGAATTAAAAATTTCTCAGTTGTTTGCGGAat                                                  >  2:45988/1‑150 (MQ=255)
                                                                                                     gTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGaaa                                                  >  2:798829/1‑149 (MQ=255)
                                                                                                     gTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAat                                                  >  2:540917/1‑150 (MQ=255)
                                                                                                          cagcagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCt                                             >  1:1472138/1‑150 (MQ=255)
                                                                                                          cagcagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCt                                             >  1:2020226/1‑150 (MQ=255)
                                                                                                          cagcagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGAGGTCAATAAGGACATGGTATAAAGTGTATTATTTCTTCGCTTCTAAGCCATCAGTTTCAGAGAGAATTAAAAATTTCTAATTTGTTTGCGAAATATTCt                                             >  2:2050242/1‑150 (MQ=255)
                                                                                                           tgtagGTTATTGTTTTTATTTCCGCAATTAAACGACCCCGTATTATACGGGGTCAATAAGGACATGGTATATAGGGTATTATTTCTTCGCTTCTTCGCCATCAGTTTCAGAGGGATTTAAAAATTTATCAGTTGTTTGCGGAATATTCTg                                            <  2:1180372/147‑1 (MQ=255)
                                                                                                              agGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACTGGGTCAATAATGAAATGGTATAAAGAGTATTATTTCTTCGCTTATACCCCAACAGTTTCAGAGCGAAAAAAAAATTTCTAATTTGTTAGCGGAATATTCTGAAt                                         >  1:112954/1‑150 (MQ=255)
                                                                                                              agGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAAt                                         >  1:332906/1‑150 (MQ=255)
                                                                                                               ggATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTATGAATc                                        >  2:1211615/1‑150 (MQ=255)
                                                                                                                 atatCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAg                                      <  1:689454/150‑1 (MQ=255)
                                                                                                                   atCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGcc                                    >  2:532074/1‑150 (MQ=255)
                                                                                                                   atCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGcc                                    <  2:1619059/150‑1 (MQ=255)
                                                                                                                   atCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGcc                                    <  1:315541/150‑1 (MQ=255)
                                                                                                                   atCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGcc                                    >  1:401723/1‑150 (MQ=255)
                                                                                                                      gTTTTTAATTCCGCACAAAAACGACCACGTAATATACGGGGTCAATAAGTACATGGTATAAAGGGTATTATTTCTACGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCTGAATATTCTGAATCAGCCatt                                 >  1:94611/1‑148 (MQ=255)
                                                                                                                        ttttAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCg                               >  2:279468/1‑150 (MQ=255)
                                                                                                                            aaTTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCa                           <  1:1249173/150‑1 (MQ=255)
                                                                                                                            aaTTCCGCACAAAAACGACCCCGTAACATACGGGGTCAATAAGGACATGGTTTAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCa                           >  2:1638948/1‑150 (MQ=255)
                                                                                                                             aTTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCAt                          >  2:967873/1‑150 (MQ=255)
                                                                                                                                  gCACAAAAACGACCCCTTAATATACGTGGTAAATAAGGACATGGTATAAAGGGAATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAACTTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCt                     <  1:44252/150‑1 (MQ=255)
                                                                                                                                    acaacaACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCttt                   <  1:1287045/150‑1 (MQ=255)
                                                                                                                                    acaAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCttt                   >  1:152928/1‑150 (MQ=255)
                                                                                                                                     ccttattgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTGGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCtttt                  >  1:1814747/8‑150 (MQ=255)
                                                                                                                                     caAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCtttt                  <  1:1938327/150‑1 (MQ=255)
                                                                                                                                      cttattgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTc                 >  1:866070/7‑150 (MQ=255)
                                                                                                                                       aaaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTCCGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTcc                >  2:1912127/1‑150 (MQ=255)
                                                                                                                                        tattgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCt               >  2:497000/5‑150 (MQ=255)
                                                                                                                                          aCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCa             >  1:1856955/1‑150 (MQ=255)
                                                                                                                                           tgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCAt            >  1:1677113/2‑150 (MQ=255)
                                                                                                                                              ccccGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTa         <  2:1067373/150‑1 (MQ=255)
                                                                                                                                               cccGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAATAATTTCTCAGTTGGTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTaa        <  1:1041323/150‑1 (MQ=255)
                                                                                                                                                ccGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTaaa       >  1:744613/1‑150 (MQ=255)
                                                                                                                                                ccGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTaaa       <  1:616813/150‑1 (MQ=255)
                                                                                                                                                 cGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAAATTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCAGTTGCTTTTCCTCATTTaaaa      <  2:1111581/150‑1 (MQ=255)
                                                                                                                                                   tAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAAta    >  2:1887653/1‑149 (MQ=255)
                                                                                                                                                   tAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATc    <  2:1740338/150‑1 (MQ=255)
                                                                                                                                                     atatACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGc  <  1:541155/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGC  >  NC_000913/1314533‑1314831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: