Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,314,684 A→T 7.1% intergenic (+26/+34) ompW → / ← yciE outer membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,314,6840AT 7.1% 343.9 / 11.8 124intergenic (+26/+34)ompW/yciEouter membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (72/43);  new base T (7/2);  total (79/45)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 127 total aligned reads displayed.

ATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTT  >  NC_000913/1314536‑1314833
                                                                                                                                                    |                                                                                                                                                     
aTGTCAGTGGGGTCCATGGATATCGATACCACCGCCAATTATAAGCGGGCCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAatat                                                                                                                                                      <  2:2038058/150‑1 (MQ=255)
  gTCAGTGTGGTACTTGGATATCGATACATACGCAATTTATATGCTGGGCGGTGGACAGCAAGACGATAGCGTAATCCTCGATCCTTGGGTGTTTATGTTCTCTGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATAc                                                                                                                                                    <  2:2002286/150‑1 (MQ=255)
    cAGTGTGGTACATGGATATCGCTACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGCTGTTTATGTTCTCAGCAGGATATCGATTTTAATTCCGCAAAAAAACGACCCCGTAAAAAACgg                                                                                                                                                  >  1:107399/1‑150 (MQ=255)
    cAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACgg                                                                                                                                                  >  2:601558/1‑150 (MQ=255)
     aGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTATTATACggg                                                                                                                                                 >  2:798209/1‑150 (MQ=255)
     aGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACggg                                                                                                                                                 >  2:1249173/1‑150 (MQ=255)
      ttgtgGTACATGGAAATCGATACCACCGCCAATTATAAGCTGGTCGTTGCACAGCAACAAGATAGCGTACGCCTCGATCCGGGGGTGTTATTGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACgggg                                                                                                                                                <  1:1590934/149‑1 (MQ=255)
      gtgtGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACgggg                                                                                                                                                <  1:1095054/150‑1 (MQ=255)
       tgtgGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGt                                                                                                                                               <  1:1202048/150‑1 (MQ=255)
          ggTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCaa                                                                                                                                            >  2:1938327/1‑150 (MQ=255)
          ggTACATGGATATCGAAACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTACGCACAAAAACGACCCAGTAATATACGGGGTCaa                                                                                                                                            >  1:1837233/1‑150 (MQ=255)
           gTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGCCCCCGTAATATACGGGGTCAAt                                                                                                                                           >  2:341689/1‑150 (MQ=255)
           ctACATGGATATCGACACCACCGCCAATTAAAAGCTGGGCGGTGAACAGCAACACCAAAGCGTACGGCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTATATTACGGGGTcgtt                                                                                                                                           <  2:387400/149‑4 (MQ=255)
             aCATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATaa                                                                                                                                         >  1:2102160/1‑150 (MQ=255)
              cATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGTCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAg                                                                                                                                        >  2:1685372/1‑150 (MQ=255)
                    aatCGATACCACCGCAAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCAGTGGGTGGCTATGTTCTCAGCAGGATATCGTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATgg                                                                                                                                 <  2:76489/149‑1 (MQ=255)
                     atCGATACCACCGCCAATTATAAGCTGTGCGGTGCACATCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTGTAAGAAGGATATCTTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATgg                                                                                                                                 <  2:1739190/150‑1 (MQ=255)
                     atCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATgg                                                                                                                                 <  1:695854/150‑1 (MQ=255)
                      tCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGt                                                                                                                                >  2:843429/1‑150 (MQ=255)
                         aTACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGtata                                                                                                                             >  2:393616/1‑150 (MQ=255)
                            ccaccGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAg                                                                                                                          >  1:941470/1‑150 (MQ=255)
                            ccaccGCCAATTATAAGATGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTAAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAg                                                                                                                          >  2:315541/1‑150 (MQ=255)
                             caccGCCAATTATAAGCTGGGTGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAgg                                                                                                                         >  1:1740338/1‑150 (MQ=255)
                              accGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTCCGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAggg                                                                                                                        <  2:96871/150‑1 (MQ=255)
                               ccGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGt                                                                                                                       >  1:511457/1‑150 (MQ=255)
                               ccGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTAAATTCCTCACAAAAACGACCCCGTAATATACTCGGTCAATAAGGACATGGTATAAAGGGt                                                                                                                       >  1:1154155/1‑150 (MQ=255)
                                  ccAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCATCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAATGACATGGTATAAAGGGtttt                                                                                                                    >  2:296066/1‑150 (MQ=255)
                                     aTTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTattatt                                                                                                                 >  2:616813/1‑150 (MQ=255)
                                          aaGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATttcttg                                                                                                            >  2:395219/1‑149 (MQ=255)
                                              tGGGCGGTGCACAGAAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACATAAACGACCCCGTAATATACGTGGTGAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCtt                                                                                                        <  1:47369/150‑1 (MQ=255)
                                               gggCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGACAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTc                                                                                                       >  2:2079594/1‑150 (MQ=255)
                                                  cGGTGCACAGCAACACGATAGCGTACGCCTCGCTCCGTGGGTGTTTATGTTCGCAGCAGGATATCGTTTTTAATTCCGCACAAAAAAGACCCCGTAATATACGGGGGCAATAAGGACATGGTAAAAAGGGGATTATTTCTTCGATTCTAc                                                                                                    >  1:833030/1‑150 (MQ=255)
                                                      gCACAGCAACACGATAGCGGACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCa                                                                                                <  1:185394/150‑1 (MQ=255)
                                                       cacaGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCAt                                                                                               >  2:2102424/1‑150 (MQ=255)
                                                        acaGCAACACGAAAGCGTACGCCTCGATCCGTGGGTGGTTATGTGCTCAGCAGGATATCGTTTTTATTTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTCCGCTTCTACGCCATc                                                                                              <  1:125167/150‑1 (MQ=255)
                                                         caGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATTCGGGGTCAATAATGACATGGTTTAAAGGGTATTATTTCTTCGCTTCTACGCCATCa                                                                                             >  2:141487/1‑150 (MQ=255)
                                                         caGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATcg                                                                                             >  2:236282/1‑149 (MQ=255)
                                                         caGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCa                                                                                             <  1:1738012/150‑1 (MQ=255)
                                                         caGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAAGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCa                                                                                             >  2:338192/1‑150 (MQ=255)
                                                          aGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAg                                                                                            <  2:1541847/150‑1 (MQ=255)
                                                            cAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGACAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCATCTACGCCATAAGtt                                                                                          >  1:1180372/1‑150 (MQ=255)
                                                                acGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCag                                                                                      >  1:1127340/1‑150 (MQ=255)
                                                                acGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCag                                                                                      <  1:721721/150‑1 (MQ=255)
                                                                   aTAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGc                                                                                   <  2:189810/150‑1 (MQ=255)
                                                                        gTACGCCTCGATGCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAAtt                                                                              <  2:1092542/150‑1 (MQ=255)
                                                                         tACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTa                                                                             >  2:1155544/1‑150 (MQ=255)
                                                                         tACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTa                                                                             >  2:1503323/1‑150 (MQ=255)
                                                                            gCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATTTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaaa                                                                          >  1:1840968/1‑150 (MQ=255)
                                                                            gCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGGAATATACGGGGTCAATAAGGACATGGTATAAAGGGTAGTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaaa                                                                          >  2:1960436/1‑150 (MQ=255)
                                                                             ccTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaaaa                                                                         >  1:2101880/1‑150 (MQ=255)
                                                                                 gATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtc                                                                     <  2:68415/150‑1 (MQ=255)
                                                                                 gATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtc                                                                     <  1:328547/150‑1 (MQ=255)
                                                                                           tgtTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGAGAATTAAAAATTTCACAGTTGttt                                                           >  2:2065983/1‑150 (MQ=255)
                                                                                             tttATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGc                                                         <  1:260752/150‑1 (MQ=255)
                                                                                              ttATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCg                                                        >  1:2019461/1‑150 (MQ=255)
                                                                                              ttATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCg                                                        >  2:1608470/1‑150 (MQ=255)
                                                                                              ttATGTTCTCAGAAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGGATCAGAGCGAATTAAAAATTTCTCACTTGTTTGCg                                                        <  2:1885667/150‑1 (MQ=255)
                                                                                               tttgttgtCATCAGTATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGTGTCAATTAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTTAAAATTTCTCAGTTGTTTGCgg                                                       <  2:983925/148‑1 (MQ=255)
                                                                                                 tGTTCTCAGCAGGATATCGTTTTTAATTACGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGAGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGATTTTTGCGGaa                                                     >  1:1308799/1‑150 (MQ=255)
                                                                                                  gTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGATCGAATTAAAAATTTCTCAGTTGTTTGCGGAat                                                    >  2:45988/1‑150 (MQ=255)
                                                                                                  gTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGaaa                                                    >  2:798829/1‑149 (MQ=255)
                                                                                                       cagcagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCt                                               >  1:1472138/1‑150 (MQ=255)
                                                                                                       cagcagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCt                                               >  1:2020226/1‑150 (MQ=255)
                                                                                                       cagcagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCt                                               <  2:1206490/150‑1 (MQ=255)
                                                                                                       cagcagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGAGGTCAATAAGGACATGGTATAAAGTGTATTATTTCTTCGCTTCTAAGCCATCAGTTTCAGAGAGAATTAAAAATTTCTAATTTGTTTGCGAAATATTCt                                               >  2:2050242/1‑150 (MQ=255)
                                                                                                        tgtagGTTATTGTTTTTATTTCCGCAATTAAACGACCCCGTATTATACGGGGTCAATAAGGACATGGTATATAGGGTATTATTTCTTCGCTTCTTCGCCATCAGTTTCAGAGGGATTTAAAAATTTATCAGTTGTTTGCGGAATATTCTg                                              <  2:1180372/147‑1 (MQ=255)
                                                                                                           agGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAAt                                           >  1:332906/1‑150 (MQ=255)
                                                                                                            ggATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTATGAATc                                          >  2:1211615/1‑150 (MQ=255)
                                                                                                              atatCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAg                                        <  1:689454/150‑1 (MQ=255)
                                                                                                               tatCGTTTTTAATTCCGCACAAAAACGACCCCGTAATAAACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGc                                       <  1:1516950/150‑1 (MQ=255)
                                                                                                                atCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGcc                                      >  2:532074/1‑150 (MQ=255)
                                                                                                                atCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGcc                                      <  2:1619059/150‑1 (MQ=255)
                                                                                                                   gTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGAATTATTTCTTCGCTTCAACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGt                                   >  2:544471/1‑150 (MQ=255)
                                                                                                                   gTTTTTAATTCCGCACAAAAACGACCACGTAATATACGGGGTCAATAAGTACATGGTATAAAGGGTATTATTTCTACGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCTGAATATTCTGAATCAGCCatt                                   >  1:94611/1‑148 (MQ=255)
                                                                                                                         aaTTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTGAGGTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCa                             >  1:260586/1‑150 (MQ=255)
                                                                                                                         aaTTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCa                             <  1:1249173/150‑1 (MQ=255)
                                                                                                                          aTTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCAt                            >  2:967873/1‑150 (MQ=255)
                                                                                                                          aTTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCAt                            >  1:507027/1‑150 (MQ=255)
                                                                                                                               gCACAAAAACGACCCCTTAATATACGTGGTAAATAAGGACATGGTATAAAGGGAATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAACTTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCt                       <  1:44252/150‑1 (MQ=255)
                                                                                                                                 acaacaACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCttt                     <  1:1287045/150‑1 (MQ=255)
                                                                                                                                  caAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCtttt                    <  1:1938327/150‑1 (MQ=255)
                                                                                                                                  caAAAACGACCCCGTAATATACGGGGTCAATAAGGACAAGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTACTCAGTTGTTTGAGGAATATTCTTAATCAGCCAGTCATCCATTTGCtttt                    >  1:391471/1‑150 (MQ=255)
                                                                                                                                   cttattgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTc                   >  1:866070/7‑150 (MQ=255)
                                                                                                                                    ttattgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTcc                  >  1:63142/6‑150 (MQ=255)
                                                                                                                                    aaaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTCCGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTcc                  >  2:1912127/1‑150 (MQ=255)
                                                                                                                                    aaaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTcc                  >  1:1274951/1‑150 (MQ=255)
                                                                                                                                     tattgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCt                 >  2:497000/5‑150 (MQ=255)
                                                                                                                                       aCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCa               >  1:1856955/1‑150 (MQ=255)
                                                                                                                                        tgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCAt              >  1:1677113/2‑150 (MQ=255)
                                                                                                                                        tgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTATCAGTTGTTTGCGGAATATTCTGAATCAGCCAGACGGCCATTTGCTTTTCCTCAt              >  2:1412778/2‑150 (MQ=255)
                                                                                                                                           ccccGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTa           <  2:283175/150‑1 (MQ=255)
                                                                                                                                           ccccGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTa           <  2:1067373/150‑1 (MQ=255)
                                                                                                                                            cccGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAATAATTTCTCAGTTGGTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTaa          <  1:1041323/150‑1 (MQ=255)
                                                                                                                                             ccGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTaaa         <  1:616813/150‑1 (MQ=255)
                                                                                                                                             ccGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTaaa         >  1:744613/1‑150 (MQ=255)
                                                                                                                                              cGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAAATTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCAGTTGCTTTTCCTCATTTaaaa        <  2:1111581/150‑1 (MQ=255)
                                                                                                                                                tAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATc      <  2:1740338/150‑1 (MQ=255)
                                                                                                                                                 aaTATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGCATTAAAAATTTCTCAGTTGTTTGCGGAATATTATGAATCAGCCAGTCGGCCATTTGATTTTCCTCATTTAAAATCg     >  1:1210509/1‑150 (MQ=255)
                                                                                                                                                  atatACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGc    <  1:541155/150‑1 (MQ=255)
                                                                                                                                                    ataCGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCtt  <  1:393616/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCTT  >  NC_000913/1314536‑1314833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: