Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 126,075 T→G 6.5% V127V (GTT→GTG aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913126,0750TG 6.5% 301.0 / 3.4 110V127V (GTT→GTGaceFpyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
Reads supporting (aligned to +/- strand):  ref base T (49/51);  new base G (4/5);  total (53/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

GCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGT  >  NC_000913/125932‑126221
                                                                                                                                               |                                                                                                                                                  
gcagcaGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGAt                                                                                                                                              >  2:1297858/1‑150 (MQ=255)
 cagcagACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATa                                                                                                                                             >  1:1317975/1‑150 (MQ=255)
  agcagACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCGGCGCCGAAGTTGAAGTGACTGAAGTGCTGGTGATAGTGGGCGACaa                                                                                                                                            >  1:384904/1‑150 (MQ=25)
   gcagACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGCAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAGGTTGACGTGACCGCAGTCCTGGTGAAAGTTGGCGAGaaa                                                                                                                                           >  1:550255/1‑150 (MQ=255)
   gcagACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATaaa                                                                                                                                           <  2:1321518/150‑1 (MQ=255)
       ccGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTg                                                                                                                                       <  1:607835/149‑1 (MQ=255)
       aCGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTg                                                                                                                                       <  2:1719958/150‑1 (MQ=255)
       aCGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTg                                                                                                                                       <  2:680666/150‑1 (MQ=255)
        cGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGCCCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGa                                                                                                                                      >  1:1169939/1‑150 (MQ=255)
          ccgCACCTGCGCAGGCAGAACAGCAGCAAGAAGCAGCGCCCGCAGCAGCACCACCGGCGGCGGCGGGAAAAGACGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTgctg                                                                                                                                    <  2:384904/148‑4 (MQ=14)
          cTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAg                                                                                                                                    <  2:1323404/150‑1 (MQ=255)
           tGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGc                                                                                                                                   >  2:156847/1‑150 (MQ=255)
           tGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGATAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAGAACAGGAGAAAGATGACCAAAAAGATGAAga                                                                                                                                   >  1:1183144/1‑149 (MQ=255)
             ctccTTCTCAGGCATAAGAGAAGAAAGAATCTTCACCGGCAGCAGCACCAGCCGCTGCGTCGGAAATAGAATTAAACGTTCCGGATATCGGGTGTGACGAAGTTGAACTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTg                                                                                                                                 <  2:1158991/148‑1 (MQ=255)
             cACCTGCTCAGGCAGAAGATAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTg                                                                                                                                 <  2:84184/150‑1 (MQ=255)
             cACCTGCTCAGGCAGAAGAGTAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTg                                                                                                                                 <  1:404973/150‑1 (MQ=255)
              aCCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGCCGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGa                                                                                                                                >  1:1108752/1‑150 (MQ=255)
               ccTGCTCAGGCAGACGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGaa                                                                                                                               <  1:286756/150‑1 (MQ=255)
               ccTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGaa                                                                                                                               <  2:1068691/150‑1 (MQ=255)
                  gCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAATAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTCAAAGTTGGCGATAAAGTTGAAGCTGAACAg                                                                                                                            >  1:1101217/1‑150 (MQ=255)
                    tCAGGCAGACGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc                                                                                                                          <  2:1606181/150‑1 (MQ=255)
                       ggCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCt                                                                                                                       <  1:1160982/150‑1 (MQ=255)
                        gCAGAAGATAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTg                                                                                                                      <  1:804692/150‑1 (MQ=255)
                        gCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTg                                                                                                                      <  1:2038687/150‑1 (MQ=255)
                         cAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGCCGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGa                                                                                                                     >  1:1072986/1‑150 (MQ=255)
                           gaagagaagaAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGTGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATc                                                                                                                   <  1:196781/150‑1 (MQ=255)
                            aagagaagaAATAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCa                                                                                                                  <  2:349423/150‑1 (MQ=255)
                                taagaaagaAGCAGCTCCGGCAGCAGCACCAGCTGCTGCGGGGGCAAAAGACGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGt                                                                                                              <  2:865056/149‑1 (MQ=11)
                                gaagaaAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGAGGAAGTTGAAGTAACCGAAATCCTGGTGAAAGTTGTAGATAAAGTTGAAGCTGAACAGTAGCTGATCACCGt                                                                                                              >  2:1486571/1‑150 (MQ=37)
                                 aagaaagaAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTa                                                                                                             <  1:604321/150‑1 (MQ=255)
                                  agaaagaaGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAg                                                                                                            <  1:748716/150‑1 (MQ=255)
                                  agaaagaaGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAg                                                                                                            >  1:410401/1‑150 (MQ=255)
                                  agaaagaaGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAg                                                                                                            >  1:1312184/1‑150 (MQ=255)
                                   gaaagaaGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAGGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGa                                                                                                           >  1:1595845/1‑150 (MQ=255)
                                     aagaagCAGCTCCGGCAGCAGCAACAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGCCCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg                                                                                                         >  2:854321/1‑150 (MQ=255)
                                       gaagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGc                                                                                                       <  2:1317975/150‑1 (MQ=255)
                                       gaagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGc                                                                                                       <  1:1379282/150‑1 (MQ=255)
                                         agcagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGa                                                                                                     >  1:2097681/1‑150 (MQ=255)
                                          gcagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGAc                                                                                                    <  1:1393249/150‑1 (MQ=255)
                                          gcaccgccGGCTGCCGCAGCAGCGGCTGCGGCGGGAAAAGAAGTTAACGTTCCGGATATCGGCTGTGACGAAGGTGAAGTGACTGAATTTATGGTGAAAGTTGGCGAAAAAGTTGAAGCTGAACAGTCACTGATCACCGTAGAAGGCGAc                                                                                                    <  2:1364705/144‑1 (MQ=14)
                                           cagcTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACa                                                                                                   <  2:1801531/150‑1 (MQ=255)
                                              cTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAgg                                                                                                >  1:1778676/1‑150 (MQ=255)
                                                ccGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt                                                                                              >  1:1198329/1‑150 (MQ=255)
                                                      gcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCTCTGATCACCGTAGAAGGCGACAAGGCTTCTATg                                                                                        >  2:1567912/1‑150 (MQ=255)
                                                      gcagcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATg                                                                                        <  1:1459948/150‑1 (MQ=255)
                                                         gcaCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCGGGGACGAAGTTGAAGTGACCGAAGTGCTGGTGAAAGTTGGCGATAAAGTTGCAGCTGAAGAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGat                                                                                     >  2:596134/1‑149 (MQ=17)
                                                              aGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTcc                                                                                >  2:2036614/1‑150 (MQ=255)
                                                               gCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCg                                                                               >  1:359623/1‑150 (MQ=255)
                                                                 ggCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGc                                                                             <  1:1548062/150‑1 (MQ=255)
                                                                   cTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTc                                                                           <  1:877995/150‑1 (MQ=255)
                                                                    tGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTcc                                                                          >  2:307657/1‑150 (MQ=255)
                                                                       ggcggcAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGtt                                                                       <  1:652350/150‑1 (MQ=255)
                                                                             aaaaGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTgg                                                                 <  2:1865412/150‑1 (MQ=255)
                                                                                  aCGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCg                                                            <  2:548679/150‑1 (MQ=255)
                                                                                    gTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGAGAAAGTTGAAGCTGAACAGTCGCTGATAACCGAACGAGCCGACAAGGCATCTATGCAAGTTCCGGCTGCGTTTGCTGGCAACGTg                                                          >  1:1611135/1‑150 (MQ=18)
                                                                                     ttAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGa                                                         <  1:1470028/150‑1 (MQ=255)
                                                                                      tAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAACTCCTGGTGAAAGTTGGCGAAAAAGTTGCAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTAGCAGGCAGCGTGaa                                                        >  1:1398820/1‑150 (MQ=17)
                                                                                        aagtaccGGACATCGGCTCAGATGAAGTTGAAATGACCGAGATCCTGGTCAAAGTTGGCGAAAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGTCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAag                                                      <  1:1194317/145‑1 (MQ=14)
                                                                                         cGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTGTGCGAGAAAGTTGAAGCTGAACAGTCGCTGATCAACGTAGAAGGCGACAACGCTTCTATGGAAGTTCCGGCGCCGTTTACAGGCGACGTGAAgga                                                     >  1:1363178/1‑150 (MQ=14)
                                                                                          gTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAagag                                                    <  1:650957/150‑1 (MQ=255)
                                                                                          gTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACAGAAATCCTGGTGAAAGTTGACGAAAAAGTTGCAGCAGAAAAGTCGCTCATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCATGCTCCGTTTGCTGGCACCGTGATagag                                                    >  1:1583610/1‑150 (MQ=18)
                                                                                             ccGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATc                                                 <  2:1726345/150‑1 (MQ=255)
                                                                                             ccGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGAGAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCTACGTGAAGGAAATc                                                 >  2:207070/1‑150 (MQ=18)
                                                                                                  tatCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGt                                            <  2:1890601/150‑1 (MQ=255)
                                                                                                    tCGGCAGCGACGAAGTTGAAGGGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGa                                          <  2:1899357/150‑1 (MQ=255)
                                                                                                      ggCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAAc                                        >  2:1430709/1‑150 (MQ=255)
                                                                                                       gCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACg                                       >  2:522743/1‑150 (MQ=255)
                                                                                                       gCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACg                                       <  1:1242913/150‑1 (MQ=255)
                                                                                                         aGCGACGAAGTTGAAGTGACCGAAATCCTGGTTAAATTTGGCTATAATGTTGAAGCGTATCTGTCTCTGTTCACCGTAGAAGGCGTCAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTg                                     <  1:867919/150‑1 (MQ=25)
                                                                                                         aGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTg                                     <  2:835615/150‑1 (MQ=255)
                                                                                                           cgacgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGATCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTggg                                   >  1:112510/1‑150 (MQ=255)
                                                                                                           cgacgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGACGTGAAAGACCTGAAAGTCAACGTtgg                                   >  2:857107/1‑150 (MQ=17)
                                                                                                           cgacgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTggg                                   <  1:2036614/150‑1 (MQ=255)
                                                                                                           cgacgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTggg                                   >  1:545394/1‑150 (MQ=255)
                                                                                                            gacgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGATGAAGCTGAACAGTAGCTGATCACCGTAGAAGGCGACAAGTCTACTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGt                                  >  2:457081/1‑150 (MQ=255)
                                                                                                                aaGTTGAAGTGACCGAAATCCTGCTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACa                              <  1:86520/150‑1 (MQ=255)
                                                                                                                   ttGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAg                           <  2:1072986/150‑1 (MQ=255)
                                                                                                                     gAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAgtg                         >  1:1754456/1‑150 (MQ=255)
                                                                                                                     gAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAACGCATCGATGGAAGGTCCGGCGCCGTTTGCTGGCGGCGTGAACGAGCTGAAAGGGTACGTGGGGGCAAAAgtg                         >  2:1846477/1‑150 (MQ=17)
                                                                                                                     gAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTc                                                                                            <  2:455278/83‑1 (MQ=255)
                                                                                                                      aaGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCAGATTTGAAGGCGACAAGTCTTATATGGAAGATCAGTCTCAGTTTGATGGCACAGTTAAAGAGATAAAAGTGAACGTGGGTGACAAAgtgt                        >  1:1169426/1‑150 (MQ=255)
                                                                                                                      aaGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAgtgt                        <  1:576569/150‑1 (MQ=255)
                                                                                                                       aatcaCCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAag                                                      <  2:452968/115‑1 (MQ=11)
                                                                                                                       aatcaCCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAACGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAag                                                      >  1:452968/5‑119 (MQ=11)
                                                                                                                       aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGACCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTc                       >  2:1232844/1‑150 (MQ=255)
                                                                                                                       aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTc                       >  2:963378/1‑150 (MQ=255)
                                                                                                                       aGTGACCGAAATCCTGGTGAAAGTAGGCGATAAAGTTGAAGCTGAACAGGCGCTGAGCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTc                       <  1:1281909/150‑1 (MQ=255)
                                                                                                                         tcaCCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACg                                       >  2:1790154/3‑132 (MQ=14)
                                                                                                                         tcaCCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACg                                       <  1:1790154/130‑1 (MQ=14)
                                                                                                                         tGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTGTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTa                     >  1:1289291/1‑150 (MQ=255)
                                                                                                                           aCCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTAcc                   <  1:592810/150‑1 (MQ=255)
                                                                                                                                aaTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTc              >  2:1321781/1‑150 (MQ=255)
                                                                                                                                    cTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACAGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGATCAGTTTGATGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGcgg          >  1:2087744/1‑148 (MQ=255)
                                                                                                                                     tGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCTCTGATCACAGTATAAGGCTACAAGCCTTCTATGGAAGTTCCGTCTCCGTTTGCTGGCACCGGGAAAGAGATCAACGTGCACGTGGGTGACGAAGTGTCTGCCGGCTCGcggg         >  2:344474/1‑147 (MQ=255)
                                                                                                                                        tcaaaGTGGGCGACAAAGTTGAAGCGGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCACTGATTa      <  1:877683/148‑1 (MQ=255)
                                                                                                                                        tGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTa      <  2:1988916/150‑1 (MQ=255)
                                                                                                                                         gAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTAt     >  1:892420/1‑150 (MQ=255)
                                                                                                                                           aaGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATgg   <  1:1518942/150‑1 (MQ=255)
                                                                                                                                           aaGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATgg   <  2:1494281/150‑1 (MQ=255)
                                                                                                                                            aGTTGGCGATAAAGTTGAAGCTGATCAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGt  <  2:573452/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
GCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGT  >  NC_000913/125932‑126221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: